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An Experimental Research on Different Detection Conditions

Between MTT and CCK-8 £¨1.School of Physics and Telecommunication Engineering, South China Normal University,Guangzhou510006,Guangdong,China;2.The Key Laboratory of Biomedical Information Engineering of Ministry of Education,School of Life Science and Technology , Xi¡¯an Jiaotong University, Xi¡¯an 710049,Shann xi,China £©


Abstract£ºThe different detection conditions based on HL60 between MTT and CCK-8 were studied. The best test time is 4h, the best test wavelength is 490nm for CCK-8. The linearity of CCK-8 between OD and cell viability was better than those of MTT. CCK-8 was a kind of cell viability assay reagent with a higher sensitivity and a better reproducibility.

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