cl/cl/*ID EU626136; SV 1; circular; other DNA; STD; SYN; 5029 BP. AC EU626136; DT 24-AUG-2008 (Rel. 96, Created) DT 24-AUG-2008 (Rel. 96, Last updated, Version 1) DE Vector mini-Tn7-Tel, complete sequence. KW . OS Vector mini-Tn7-Tel OC other sequences; artificial sequences; vectors. RN [1] RP 1-5029 RA Kang Y., Barrett A.R., Son M.S.; RT "Genetic systems for single copy analysis based on non-antibiotic RT selectable marker in Burkholderia spp."; RL Unpublished. RN [2] RP 1-5029 RA Kang Y., Barrett A.R., Son M.S.; RT ; RL Submitted (06-APR-2008) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Hawaii at Manoa, 2538 McCarthy Mall Snyder Hall RL Rm 310, Honolulu, HI 96822, USA FH Key Location/Qualifiers FT source 1..5029 FT /organism="Vector mini-Tn7-Tel" FT /mol_type="other DNA" FT /db_xref="taxon:554284" FT repeat_region 146..344 FT /mobile_element="integron" FT /note="Tn7R" FT terminator 394..487 FT /note="T0 terminator" FT terminator 585..681 FT /note="T1 terminator" FT misc_recomb 722..769 FT /note="FRT; Flp recombination target" FT terminator 830..861 FT /note="TonB terminator" FT gene complement(879..1832) FT /gene="telB" FT CDS complement(879..1832) FT /codon_start=1 FT /transl_table=11 FT /gene="telB" FT /product="TelB" FT /note="confers tellurite resistance" FT /protein_id="ACH42103.1" FT /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV FT TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF FT TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP FT SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG FT SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG FT AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP" FT gene complement(1829..2965) FT /gene="telA" FT CDS complement(1829..2965) FT /codon_start=1 FT /transl_table=11 FT /gene="telA" FT /product="TelA" FT /note="confers tellurite resistance" FT /protein_id="ACH42104.1" FT /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE FT VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN FT RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA FT VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD FT LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL FT ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ FT EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV" FT gene complement(2983..3756) FT /gene="kilA" FT CDS complement(2983..3756) FT /codon_start=1 FT /transl_table=11 FT /gene="kilA" FT /product="KilA" FT /note="confers tellurite resistance" FT /protein_id="ACH42105.1" FT /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL FT MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL FT LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF FT DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA FT LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ" FT promoter complement(3796..3837) FT /note="Burkholderia cenocepacia rpsL promoter; PCS12" FT misc_recomb 3877..3924 FT /note="FRT; Flp recombination target" FT repeat_region 4037..4202 FT /mobile_element="integron" FT /note="Tn7L" FT rep_origin 4366..4741 FT /note="R6K-gamma origin of replication" SQ Sequence 5029 BP; 1024 A; 1441 C; 1408 G; 1156 T; 0 other; cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 60 ctattacgcc agctagagga ccagccgcgt aacctggcaa aatcggttac ggttgagtaa 120 taaatggatg ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa 180 tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca 240 gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca 300 ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggggttcg aggtcgacgg 360 tatcgataag ctagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac 420 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 480 tgagaatcca agctagactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt 540 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg 600 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 660 cctgagtagg acaaatccgc cgctaggagc ttgcggcccg gacgatcgaa ttggggatct 720 tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt 780 gaagctgatg tgcttaaaaa cttactcaat ggaattagct cgagcagaaa gtcaaaagcc 840 tccgaccgga ggcttttgac ttgaggggga tcgatccctt atggctctgc acccggctcc 900 atcaccaaca ggtcgcgcac gcgcttcact cggttgcgga tcgacactgc cagcccaaca 960 aagccggttg ccgccgccgc caggatcgcg ccgatgatgc cggccacacc ggccatcgcc 1020 caccaggtcg ccgccttccg gttccattcc tgctggtact gcttcgcaat gctggacctc 1080 ggctcaccat aggctgaccg ctcgatggcg tatgccgctt ctccccttgg cgtaaaaccc 1140 agcgccgcag gcggcattgc catgctgccc gccgctttcc cgaccacgac gcgcgcacca 1200 ggcttgcggt ccagaccttc ggccacggcg agctgcgcaa ggacataatc agccgccgac 1260 ttggctccac gcgcctcgat cagctcttgc actcgcgcga aatccttggc ctccacggcc 1320 gccatgaatc gcgcacgcgg cgaaggctcc gcagggccgg cgtcgtgatc gccgccgaga 1380 atgcccttca ccaagttcga cgacacgaaa atcatgctga cggctatcac catcatgcag 1440 acggatcgca cgaacccgca gaactcaccc ccgaacacga gcacggcacc cgcgaccact 1500 atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg 1560 acggccgaag tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg 1620 tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg 1680 ctgatcgccc atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgccgcg 1740 atgaggaagc gggtgccccg cttcttcatc ttcgcgcctc gggcctccag gccgcctacc 1800 tgggcgaaaa catcggtgtt tgtggcattc atacggactc ctgttgggcc agctcgcgca 1860 cgggctggcg ggtcagcttg gcttgaagat cgccacgcat tgcggcgatc tgcttctcgg 1920 catccttgcg cttctgcacg ccttcctgct ggatgcgaat aacgtcctcg acggtcttga 1980 tgagcgtcgt ctgaacctgc ttgagcgtgt ccacgtcgat caccaggcgt tggttctcct 2040 tcgccgtctc gacggacgtg cgatgcagca gggccgcatt gcgcttcatc aggtcgttgg 2100 tggtgtcgtc gatggccgtg gccagttcga cggcgttctt ctgctcgttg aggctcaagg 2160 ccagcatgaa ttgccgcttc cacgccggca cggtgatttc gcggatggtg tggaatttat 2220 cgaccagcat ctggttgttg gcctggatca tgcggatggt cggcaggctc tgcatggccg 2280 aatgttgcaa ggcgatcagg tcgccgatgc gcttgtccag gttggcaacc atcgcatcga 2340 ggtcggccag ctcctgcacg cggccagggt cgttcccgac attgccgcgc agaccctcgg 2400 cctgctcgcg cagctcggca aggcggacct tgccggccgc gatgtggacg ccaagaaggc 2460 ggtgttcctc gcgcacggct gcgaacattt cgtcgagcga ggcattgcgc tgcgcgatgc 2520 cttgctgggt ggtctgcact tcgctgacca ggtgttcgat ctgctcgcgg gtcgtgtcga 2580 agcgcgccat gaagcccgtc gaacggacgc ggaagcggtc gatcagcggg ccaatcaggg 2640 gcaggcggga acggttgtcg gacaaagggc cgacgttcag ggaacgggcc ttggcgacaa 2700 cctgggtcag tttctcgcct gcttcgtcca ggtcgctgtt gcgcacctgg tccagcaggc 2760 tatcggcgta gcgggacgtg tgctcggcca cgtcgcggcc gaactcggca acggtctgcg 2820 gactgccgac ctcgatccgc tgcgcgaccg catggacttc cggcacgtcg ctttcctgca 2880 agcccagctc gcgcagggtt gccggggtca tgtcgaaggc gacgataggg gccttggcgt 2940 cgtgcgtcgt tttcagtgcg ttcatagggt tctcccgccg tgttattggt tgatgccttc 3000 caggctctgc gaaaggctcc gcatgagcgc ctggtgagct ttggccgcct cggcgaccat 3060 tgccggattc atgttcttgg tggtgatgag cgcgagggtg tgctgacgcc agacgggcac 3120 caggacggat gccgtttcag agaagcggtc cagcatgtcc acggcctgcg cccgcgtgag 3180 cttcatctga gtgacgctca tttcatggga cgccatgagg gttgccaggt tggcgagctt 3240 gcgcgcgaag cgttcgcgcg gcttgtcgaa ctcgatcacg ccggccttgg ccgcgccggc 3300 ctcggggttc tcgtccagga actcgcgccc ggcttgaatg taggctctga gccggtctac 3360 ctcggcctca tgcgtattga gcatgtcatc caaggcgcgc aacgtgtccc gcacgcgctg 3420 cgctacgccc tcggcttcgt ccagcaactg gtcgagcgtc ttgcgggcga cctgatacct 3480 cacctggcgt tcaacctcac ggccaagcat cttctcgaac caggtaggct tttccgcgat 3540 cttgcggggg tccgcgtcgg ccagcttcgc cacgatctgg ctgattttgt cggccagcgc 3600 ggcaactgcg ccgtgctcca tcagattcga cagctcgttg agggaatccg ccccgtcgat 3660 gccggccccg tactcgccaa tcgtcgccgg cgacgcgaag agggcgggca aaacctcccc 3720 cttcaatcgc gccatgttca cgctttgttc ttccatggta tatctccttc ttaaagttaa 3780 acaaaattat tcggaaccca gcatgatatt ccggaaatac caactaagtc aacggctgat 3840 atccattgct gttgacaaag ggaatcaggg gatcttgaag ttcctattcc gaagttccta 3900 ttctctagaa agtataggaa cttcagagcg cttttgaagc taattcgatc atgcatgagc 3960 tcactagtgg atcccccggg ctgcaggaat tcctcgagaa gcttgggccc ggtacctcgc 4020 gaaggccttg caggccaacc agataagtga aatctagttc caaactattt tgtcattttt 4080 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct 4140 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca 4200 cagcggggca tttttcttcc tgttatgttt gggcgctgca ttaatgaatc ggccaacgcg 4260 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gacccgctgc 4320 gctcggtcgt tcggctgcgg cgagcggtat caggggattc cttaatgtca gccgttaagt 4380 gttcctgtgt cactgaaaat tgctttgaga ggctctaagg gcttctcagt gcgttacatc 4440 cctggcttgt tgtccacaac cgttaaacct taaaagctag ctttaaaagc cttatatatt 4500 cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa 4560 ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt acgttagcca 4620 tgagagctta gtacgttagc catgagggtt tagttcgtta aacatgagag cttagtacgt 4680 taaacatgag agcttagtac gtgaaacatg agagcttagt acgtactatc aacaggttga 4740 actgcatcga tgaggagctt atcggccagc ctcgcagagc aggattcccg ttgagcaccg 4800 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac 4860 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc 4920 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc 4980 cgaagcaggg ttatgcagcg gaaagtatac cttaaggaat cgataccca 5029  mini-Tn7-Tel"Times New Roman cl/(BglII(183),SalI(354),ClaI(364),NheI(371) Arialbxof6*f cl/4XbaI(750),XhoI(820),ClaI(872),SfiI(1010),SexAI(1023) ArialhQYaa cl/,SfiI(1316),EagI(1317),EagI(1563),SexAI(1616) Arial%kYuY~Y~Aq cl/"EagI(2435),SexAI(2548),SexAI(2746) Arial }.}8DDh cl/EagI Arial 7!3*/33H? cl/SfiI(3297),SexAI(3520) Arial z cl/͵NcoI(3754),EcoRV(3841),XbaI(3905),SacI(3962),SpeI(3964),BamHI(3970),XmaI(3976),SmaI(3978),PstI(3986),EcoRI(3988),XhoI(3994),HindIII(4000),ApaI(4010),KpnI(4016),NruI(4020),SfiI(4032) Arial Sk cl/NheI(4477),ClaI(4748) Arial}q cl/ClaI ArialcYOhCOcl/\lpBluescriptKS_primer"Times New Romancl/CFRT"Times New Romancl/Co(telB"Times New Romancl/eORF 1"Times New Romancl/CɆ% telA"Times New Roman*cl/C ORF 3"Times New Romancl/C@ ORF 4"Times New Romancl/C kilA"Times New Romancl/C ORF 2"Times New Romancl/CT7_transl_en_RBS"Times New Romancl/C%TFRT"Times New Romancl/CvpUni_fwd_primer"Times New Romancl/CɆ+oriT_RP4"Times New Roman