Sequence of mini-Tn7-Tel

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of mini-Tn7-Tel

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of mini-Tn7-Tel: 5029 BP; 1024 A; 1441 C; 1408 G; 1156 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 60

1 gcggtaagcg gtaagtccga cgcgttgaca acccttcccg ctagccacgc ccggagaagc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ctattacgcc agctagagga ccagccgcgt aacctggcaa aatcggttac ggttgagtaa 120

61 gataatgcgg tcgatctcct ggtcggcgca ttggaccgtt ttagccaatg ccaactcatt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 taaatggatg ccctgcgtaa gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa 180

121 atttacctac gggacgcatt cgcccacacc cgcctgttat ttcagaattt gacttgtttt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca 240

181 atctagattt gatactgtta tttcagaatt tgatctgtct tatcaacatt tgactttagt 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca 300

241 caggtcaata cgacactttt tcgtatgacc tgaaaacaat accgatttcg tttgagaagt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggggttcg aggtcgacgg 360

301 aaaagacttc acgtttaacg ggcagcataa tttctccccg caccccaagc tccagctgcc 360

.......... .......... .......... .......... ..... pBluescriptKS_primer

ClaI(364) NheI(371) .......... .......... .......... ..........

361 tatcgataag ctagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac 420

361 atagctattc gatcgaatta atcgactcga acctgaggac aactatctag gtcattactg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 480

421 gagtcttgag gtagacctaa acaagtcttg cgagccaacg gcggcccgca aaaaataacc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgagaatcca agctagactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt 540

481 actcttaggt tcgatctgac gctactcacc gtcccgcccc gcattaaaaa aattccgtca 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg 600

541 ataaccacgg gaatttgcgg accccattac tgagagatcg aactccgtag tttattttgc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 660

601 tttccgagtc agctttctga cccggaaagc aaaatagaca acaaacagcc acttgcgaga 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cctgagtagg acaaatccgc cgctaggagc ttgcggcccg gacgatcgaa ttggggatct 720

661 ggactcatcc tgtttaggcg gcgatcctcg aacgccgggc ctgctagctt aacccctaga 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt 780

721 acttcaagga taaggcttca aggataagag atctttcata tccttgaagt ctcgcgaaaa 780

.......... .......... FRT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gaagctgatg tgcttaaaaa cttactcaat ggaattagct cgagcagaaa gtcaaaagcc 840

781 cttcgactac acgaattttt gaatgagtta ccttaatcga gctcgtcttt cagttttcgg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tccgaccgga ggcttttgac ttgaggggga tcgatccctt atggctctgc acccggctcc 900

841 aggctggcct ccgaaaactg aactccccct agctagggaa taccgagacg tgggccgagg 900

.......... .......... .......... .......... telB ..........

.......... .......... .......... .......... .......... ..........

901 atcaccaaca ggtcgcgcac gcgcttcact cggttgcgga tcgacactgc cagcccaaca 960

901 tagtggttgt ccagcgcgtg cgcgaagtga gccaacgcct agctgtgacg gtcgggttgt 960

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aagccggttg ccgccgccgc caggatcgcg ccgatgatgc cggccacacc ggccatcgcc 1020

961 ttcggccaac ggcggcggcg gtcctagcgc ggctactacg gccggtgtgg ccggtagcgg 1020

.......... .......... telB .......... .......... ..........

SexAI(1023) ........ .......... .......... .......... ..........

1021 caccaggtcg ccgccttccg gttccattcc tgctggtact gcttcgcaat gctggacctc 1080

1021 gtggtccagc ggcggaaggc caaggtaagg acgaccatga cgaagcgtta cgacctggag 1080

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggctcaccat aggctgaccg ctcgatggcg tatgccgctt ctccccttgg cgtaaaaccc 1140

1081 ccgagtggta tccgactggc gagctaccgc atacggcgaa gaggggaacc gcattttggg 1140

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agcgccgcag gcggcattgc catgctgccc gccgctttcc cgaccacgac gcgcgcacca 1200

1141 tcgcggcgtc cgccgtaacg gtacgacggg cggcgaaagg gctggtgctg cgcgcgtggt 1200

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggcttgcggt ccagaccttc ggccacggcg agctgcgcaa ggacataatc agccgccgac 1260

1201 ccgaacgcca ggtctggaag ccggtgccgc tcgacgcgtt cctgtattag tcggcggctg 1260

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttggctccac gcgcctcgat cagctcttgc actcgcgcga aatccttggc ctccacggcc 1320

1261 aaccgaggtg cgcggagcta gtcgagaacg tgagcgcgct ttaggaaccg gaggtgccgg 1320

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gccatgaatc gcgcacgcgg cgaaggctcc gcagggccgg cgtcgtgatc gccgccgaga 1380

1321 cggtacttag cgcgtgcgcc gcttccgagg cgtcccggcc gcagcactag cggcggctct 1380

.......... .......... telB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atgcccttca ccaagttcga cgacacgaaa atcatgctga cggctatcac catcatgcag 1440

1381 tacgggaagt ggttcaagct gctgtgcttt tagtacgact gccgatagtg gtagtacgtc 1440

.......... .......... telB ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acggatcgca cgaacccgca gaactcaccc ccgaacacga gcacggcacc cgcgaccact 1500

1441 tgcctagcgt gcttgggcgt cttgagtggg ggcttgtgct cgtgccgtgg gcgctggtga 1500

.......... .......... telB ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg 1560

1501 tacggttctt acgggttcca tttttaacgg ccggggcggt acttcaggca cttacggggc 1560

.......... .......... telB ORF 1 .......... .......... ..........

EagI(1563) .......... .......... .......... ......... SexAI(1616)

1561 acggccgaag tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg 1620

1561 tgccggcttc acttcccgtc cggcggtggg tccggcggcg ggagtgacgg gccgtggacc 1620

.......... .......... telB ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg 1680

1621 agcgacttac agctacggtc gtggacgccg tgcagttacg aaggcccgca gcgcgagccc 1680

.......... .......... telB ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctgatcgccc atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgccgcg 1740

1681 gactagcggg tagggcaatg acggggctag ggccgttacc gttcctgacg gtcgcggcgc 1740

.......... .......... telB ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 atgaggaagc gggtgccccg cttcttcatc ttcgcgcctc gggcctccag gccgcctacc 1800

1741 tactccttcg cccacggggc gaagaagtag aagcgcggag cccggaggtc cggcggatgg 1800

.......... .......... telB ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgggcgaaaa catcggtgtt tgtggcattc atacggactc ctgttgggcc agctcgcgca 1860

1801 acccgctttt gtagccacaa acaccgtaag tatgcctgag gacaacccgg tcgagcgcgt 1860

.......... telB ORF 1 .......... telA ..........

.......... .......... .......... .......... .......... ..........

1861 cgggctggcg ggtcagcttg gcttgaagat cgccacgcat tgcggcgatc tgcttctcgg 1920

1861 gcccgaccgc ccagtcgaac cgaacttcta gcggtgcgta acgccgctag acgaagagcc 1920

.......... .......... ORF 1 telA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 catccttgcg cttctgcacg ccttcctgct ggatgcgaat aacgtcctcg acggtcttga 1980

1921 gtaggaacgc gaagacgtgc ggaaggacga cctacgctta ttgcaggagc tgccagaact 1980

.......... .......... ORF 1 telA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgagcgtcgt ctgaacctgc ttgagcgtgt ccacgtcgat caccaggcgt tggttctcct 2040

1981 actcgcagca gacttggacg aactcgcaca ggtgcagcta gtggtccgca accaagagga 2040

.......... .......... telA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcgccgtctc gacggacgtg cgatgcagca gggccgcatt gcgcttcatc aggtcgttgg 2100

2041 agcggcagag ctgcctgcac gctacgtcgt cccggcgtaa cgcgaagtag tccagcaacc 2100

.......... .......... telA .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

2101 tggtgtcgtc gatggccgtg gccagttcga cggcgttctt ctgctcgttg aggctcaagg 2160

2101 accacagcag ctaccggcac cggtcaagct gccgcaagaa gacgagcaac tccgagttcc 2160

.......... .......... telA ORF 3 ORF 4 .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ccagcatgaa ttgccgcttc cacgccggca cggtgatttc gcggatggtg tggaatttat 2220

2161 ggtcgtactt aacggcgaag gtgcggccgt gccactaaag cgcctaccac accttaaata 2220

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgaccagcat ctggttgttg gcctggatca tgcggatggt cggcaggctc tgcatggccg 2280

2221 gctggtcgta gaccaacaac cggacctagt acgcctacca gccgtccgag acgtaccggc 2280

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aatgttgcaa ggcgatcagg tcgccgatgc gcttgtccag gttggcaacc atcgcatcga 2340

2281 ttacaacgtt ccgctagtcc agcggctacg cgaacaggtc caaccgttgg tagcgtagct 2340

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggtcggccag ctcctgcacg cggccagggt cgttcccgac attgccgcgc agaccctcgg 2400

2341 ccagccggtc gaggacgtgc gccggtccca gcaagggctg taacggcgcg tctgggagcc 2400

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cctgctcgcg cagctcggca aggcggacct tgccggccgc gatgtggacg ccaagaaggc 2460

2401 ggacgagcgc gtcgagccgt tccgcctgga acggccggcg ctacacctgc ggttcttccg 2460

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggtgttcctc gcgcacggct gcgaacattt cgtcgagcga ggcattgcgc tgcgcgatgc 2520

2461 ccacaaggag cgcgtgccga cgcttgtaaa gcagctcgct ccgtaacgcg acgcgctacg 2520

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cttgctgggt ggtctgcact tcgctgacca ggtgttcgat ctgctcgcgg gtcgtgtcga 2580

2521 gaacgaccca ccagacgtga agcgactggt ccacaagcta gacgagcgcc cagcacagct 2580

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agcgcgccat gaagcccgtc gaacggacgc ggaagcggtc gatcagcggg ccaatcaggg 2640

2581 tcgcgcggta cttcgggcag cttgcctgcg ccttcgccag ctagtcgccc ggttagtccc 2640

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcaggcggga acggttgtcg gacaaagggc cgacgttcag ggaacgggcc ttggcgacaa 2700

2641 cgtccgccct tgccaacagc ctgtttcccg gctgcaagtc ccttgcccgg aaccgctgtt 2700

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... ......... SexAI(2746) .........

2701 cctgggtcag tttctcgcct gcttcgtcca ggtcgctgtt gcgcacctgg tccagcaggc 2760

2701 ggacccagtc aaagagcgga cgaagcaggt ccagcgacaa cgcgtggacc aggtcgtccg 2760

.......... ....... telA ORF 3 ORF 4 ....... .......... ..........

.......... .......... .......... EagI(2797) .......... ..........

2761 tatcggcgta gcgggacgtg tgctcggcca cgtcgcggcc gaactcggca acggtctgcg 2820

2761 atagccgcat cgccctgcac acgagccggt gcagcgccgg cttgagccgt tgccagacgc 2820

ORF 4 .......... telA ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gactgccgac ctcgatccgc tgcgcgaccg catggacttc cggcacgtcg ctttcctgca 2880

2821 ctgacggctg gagctaggcg acgcgctggc gtacctgaag gccgtgcagc gaaaggacgt 2880

.......... .......... telA ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agcccagctc gcgcagggtt gccggggtca tgtcgaaggc gacgataggg gccttggcgt 2940

2881 tcgggtcgag cgcgtcccaa cggccccagt acagcttccg ctgctatccc cggaaccgca 2940

.......... .......... telA ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgtgcgtcgt tttcagtgcg ttcatagggt tctcccgccg tgttattggt tgatgccttc 3000

2941 gcacgcagca aaagtcacgc aagtatccca agagggcggc acaataacca actacggaag 3000

.......... telA ORF 3 .......... .......... kilA

.......... .......... .......... .......... .......... ..........

3001 caggctctgc gaaaggctcc gcatgagcgc ctggtgagct ttggccgcct cggcgaccat 3060

3001 gtccgagacg ctttccgagg cgtactcgcg gaccactcga aaccggcgga gccgctggta 3060

.......... ORF 3 kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgccggattc atgttcttgg tggtgatgag cgcgagggtg tgctgacgcc agacgggcac 3120

3061 acggcctaag tacaagaacc accactactc gcgctcccac acgactgcgg tctgcccgtg 3120

.......... .......... kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 caggacggat gccgtttcag agaagcggtc cagcatgtcc acggcctgcg cccgcgtgag 3180

3121 gtcctgccta cggcaaagtc tcttcgccag gtcgtacagg tgccggacgc gggcgcactc 3180

.......... .......... kilA .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cttcatctga gtgacgctca tttcatggga cgccatgagg gttgccaggt tggcgagctt 3240

3181 gaagtagact cactgcgagt aaagtaccct gcggtactcc caacggtcca accgctcgaa 3240

.......... .......... kilA .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

3241 gcgcgcgaag cgttcgcgcg gcttgtcgaa ctcgatcacg ccggccttgg ccgcgccggc 3300

3241 cgcgcgcttc gcaagcgcgc cgaacagctt gagctagtgc ggccggaacc ggcgcggccg 3300

.......... .......... kilA ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctcggggttc tcgtccagga actcgcgccc ggcttgaatg taggctctga gccggtctac 3360

3301 gagccccaag agcaggtcct tgagcgcggg ccgaacttac atccgagact cggccagatg 3360

.......... .......... kilA ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctcggcctca tgcgtattga gcatgtcatc caaggcgcgc aacgtgtccc gcacgcgctg 3420

3361 gagccggagt acgcataact cgtacagtag gttccgcgcg ttgcacaggg cgtgcgcgac 3420

.......... .......... kilA ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgctacgccc tcggcttcgt ccagcaactg gtcgagcgtc ttgcgggcga cctgatacct 3480

3421 gcgatgcggg agccgaagca ggtcgttgac cagctcgcag aacgcccgct ggactatgga 3480

.......... .......... kilA ORF 2 .......... .......... ..........

.......... .......... ......... SexAI(3520) ......... ..........

3481 cacctggcgt tcaacctcac ggccaagcat cttctcgaac caggtaggct tttccgcgat 3540

3481 gtggaccgca agttggagtg ccggttcgta gaagagcttg gtccatccga aaaggcgcta 3540

.......... .......... kilA ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cttgcggggg tccgcgtcgg ccagcttcgc cacgatctgg ctgattttgt cggccagcgc 3600

3541 gaacgccccc aggcgcagcc ggtcgaagcg gtgctagacc gactaaaaca gccggtcgcg 3600

.......... .......... kilA ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggcaactgcg ccgtgctcca tcagattcga cagctcgttg agggaatccg ccccgtcgat 3660

3601 ccgttgacgc ggcacgaggt agtctaagct gtcgagcaac tcccttaggc ggggcagcta 3660

.......... .......... kilA ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gccggccccg tactcgccaa tcgtcgccgg cgacgcgaag agggcgggca aaacctcccc 3720

3661 cggccggggc atgagcggtt agcagcggcc gctgcgcttc tcccgcccgt tttggagggg 3720

.......... .......... kilA ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cttcaatcgc gccatgttca cgctttgttc ttccatggta tatctccttc ttaaagttaa 3780

3721 gaagttagcg cggtacaagt gcgaaacaag aaggtaccat atagaggaag aatttcaatt 3780

.......... kilA ORF 2 .......... ....... T7_transl_en_RBS

.......... .......... .......... .......... .......... ..........

3781 acaaaattat tcggaaccca gcatgatatt ccggaaatac caactaagtc aacggctgat 3840

3781 tgttttaata agccttgggt cgtactataa ggcctttatg gttgattcag ttgccgacta 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atccattgct gttgacaaag ggaatcaggg gatcttgaag ttcctattcc gaagttccta 3900

3841 taggtaacga caactgtttc ccttagtccc ctagaacttc aaggataagg cttcaaggat 3900

.......... .......... .......... .......... FRT ..........

.......... .......... .......... .......... .......... ..........

3901 ttctctagaa agtataggaa cttcagagcg cttttgaagc taattcgatc atgcatgagc 3960

3901 aagagatctt tcatatcctt gaagtctcgc gaaaacttcg attaagctag tacgtactcg 3960

.......... FRT .......... .......... .......... ..........

SacI,SpeI BamHI XmaI,SmaI PstI,EcoRI XhoI HindIII ApaI

3961 tcactagtgg atcccccggg ctgcaggaat tcctcgagaa gcttgggccc ggtacctcgc 4020

3961 agtgatcacc tagggggccc gacgtcctta aggagctctt cgaacccggg ccatggagcg 4020

.......... .......... .......... .......... .......... ..........

.......... SfiI(4032) .......... .......... .......... ..........

4021 gaaggccttg caggccaacc agataagtga aatctagttc caaactattt tgtcattttt 4080

4021 cttccggaac gtccggttgg tctattcact ttagatcaag gtttgataaa acagtaaaaa 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct 4140

4081 ttaaaagcat aatcgaatgc tgcgatgtgg gtcaagggta gataaaacag tgagaaggga 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca 4200

4141 tttattagga atttttgagg taaaggtggg gagggtcaag ggttgataaa acaggcgggt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagcggggca tttttcttcc tgttatgttt gggcgctgca ttaatgaatc ggccaacgcg 4260

4201 gtcgccccgt aaaaagaagg acaatacaaa cccgcgacgt aattacttag ccggttgcgc 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gacccgctgc 4320

4261 gcccctctcc gccaaacgca taacccgcga gaaggcgaag gagcgagtga ctgggcgacg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gctcggtcgt tcggctgcgg cgagcggtat caggggattc cttaatgtca gccgttaagt 4380

4321 cgagccagca agccgacgcc gctcgccata gtcccctaag gaattacagt cggcaattca 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gttcctgtgt cactgaaaat tgctttgaga ggctctaagg gcttctcagt gcgttacatc 4440

4381 caaggacaca gtgactttta acgaaactct ccgagattcc cgaagagtca cgcaatgtag 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cctggcttgt tgtccacaac cgttaaacct taaaagctag ctttaaaagc cttatatatt 4500

4441 ggaccgaaca acaggtgttg gcaatttgga attttcgatc gaaattttcg gaatatataa 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa 4560

4501 gaaaaaaaag aatattttga attttggaat ctccgataaa ttcaacgact aaatataatt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt acgttagcca 4620

4561 aaaataacaa gtttgtactc tcgaatcatg cactttgtac tctcgaatca tgcaatcggt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgagagctta gtacgttagc catgagggtt tagttcgtta aacatgagag cttagtacgt 4680

4621 actctcgaat catgcaatcg gtactcccaa atcaagcaat ttgtactctc gaatcatgca 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 taaacatgag agcttagtac gtgaaacatg agagcttagt acgtactatc aacaggttga 4740

4681 atttgtactc tcgaatcatg cactttgtac tctcgaatca tgcatgatag ttgtccaact 4740

.......... .......... .......... .......... ....... pUni_fwd_primer

ClaI(4748) .......... .......... .......... .......... ..........

4741 actgcatcga tgaggagctt atcggccagc ctcgcagagc aggattcccg ttgagcaccg 4800

4741 tgacgtagct actcctcgaa tagccggtcg gagcgtctcg tcctaagggc aactcgtggc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac 4860

4801 ggtccacgct tattccctgt cacttcttcc ttgtgggcga gcgcccaccc ggatgaagtg 4860

.......... .......... .......... oriT_RP4 .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc 4920

4861 gataggacgg gccgactgcg gcaacctatg tggttccttt cagatgtgct tgggaaaccg 4920

.......... .......... oriT_RP4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc 4980

4921 ttttaggaca tatagcacgc tttttcctac ctatatggct tttttagcga tattactggg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(5021) ..........

4981 cgaagcaggg ttatgcagcg gaaagtatac cttaaggaat cgataccca. .......... 5029

4981 gcttcgtccc aatacgtcgc ctttcatatg gaattcctta gctatgggt. .......... 5029

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-5029 Reference author: Kang Y., Barrett A.R., Son M.S.; Reference title: "Genetic systems for single copy analysis based on non-antibiotic Reference title: selectable marker in Burkholderia spp."; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-5029 Reference author: Kang Y., Barrett A.R., Son M.S.; Reference title: ; Reference location: Submitted (06-APR-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Microbiology, University of Hawaii at Manoa, 2538 McCarthy Mall Snyder Hall Reference location: Rm 310, Honolulu, HI 96822, USA