Sequence of p079

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Sequence of p079_pPNT6

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Sequence of p079 pPNT6 : 7501 BP; 1625 A; 2090 C; 2041 G; 1745 T; 0 other;

NotI(2) SalI(13) .......... KpnI(36) .......... ..........

1 gcggccgcac gcgtcgacag cgtttaaacg aggtaccagt ggcgcgccgg cataacttcg 60

1 cgccggcgtg cgcagctgtc gcaaatttgc tccatggtca ccgcgcggcc gtattgaagc 60

.......... .......... .......... .......... .......... loxP

.......... .......... .......... .......... .......... ..........

61 tatagcatac attatacgaa gttataggcc tgcaggtcaa ttctaccggg taggggaggc 120

61 atatcgtatg taatatgctt caatatccgg acgtccagtt aagatggccc atcccctccg 120

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctggcac ttggcgctac 180

121 cgaaaagggt tccgtcagac ctcgtacgcg aaatcgtcgg ggcgaccgtg aaccgcgatg 180

.......... .......... .......... MSCV_r_pri .......... ..........

.......... .......... .......... .......... .......... ..........

181 acaagtggcc tctggcctcg cacacattcc acatccaccg gtagcgccaa ccggctccgt 240

181 tgttcaccgg agaccggagc gtgtgtaagg tgtaggtggc catcgcggtt ggccgaggca 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg 300

241 agaaaccacc ggggaagcgc ggtggaagat gaggagggga tcagtccttc aagggggggc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg 360

301 ggggcgtcga gcgcagcacg tcctgcactg tttaccttca tcgtgcagag tgatcagagc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat 420

361 acgtctacct gtcgtggcga ctcgttacct tcgcccatcc ggaaaccccg tcgccggtta 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg 480

421 tcgtcgaaac gaggaagcga aagacccgag tctccgaccc ttccccaccc aggcccccgc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggctcagggg cgggctcagg ggcggggcgg gcgcgaaggt cctcccgagg cccggcattc 540

481 ccgagtcccc gcccgagtcc ccgccccgcc cgcgcttcca ggagggctcc gggccgtaag 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tcgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct 600

541 agcgtgcgaa gttttcgcgt gcagacggcg cgacaagagg agaaggagta gaggcccgga 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ttcgacctgc agccaatatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc 660

601 aagctggacg tcggttatac cctagccggt aacttgttct acctaacgtg cgtccaagag 660

.......... .......... ........ NeoR/KanR ORF3 ........ ..........

.......... .......... .......... .......... .......... ..........

661 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 720

661 gccggcgaac ccacctctcc gataagccga tactgacccg tgttgtctgt tagccgacga 720

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 780

721 gactacggcg gcacaaggcc gacagtcgcg tccccgcggg ccaagaaaaa cagttctggc 780

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 840

781 tggacaggcc acgggactta cttgacgtcc tgctccgtcg cgccgatagc accgaccggt 840

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 900

841 gctgcccgca aggaacgcgt cgacacgagc tgcaacagtg acttcgccct tccctgaccg 900

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 960

901 acgataaccc gcttcacggc cccgtcctag aggacagtag agtggaacga ggacggctct 960

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 1020

961 ttcataggta gtaccgacta cgttacgccg ccgacgtatg cgaactaggc cgatggacgg 1020

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 1080

1021 gtaagctggt ggttcgcttt gtagcgtagc tcgctcgtgc atgagcctac cttcggccag 1080

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 1140

1081 aacagctagt cctactagac ctgcttctcg tagtccccga gcgcggtcgg cttgacaagc 1140

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat ggcgatgcct 1200

1141 ggtccgagtt ccgcgcgtac gggctgccgc tactagagca gcactgggta ccgctacgga 1200

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 1260

1201 cgaacggctt atagtaccac cttttaccgg cgaaaagacc taagtagctg acaccggccg 1260

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 1320

1261 acccacaccg cctggcgata gtcctgtatc gcaaccgatg ggcactataa cgacttctcg 1320

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 1380

1321 aaccgccgct tacccgactg gcgaaggagc acgaaatgcc atagcggcga gggctaagcg 1380

.......... ........ NeoR/KanR ORF3 ........ .......... ..........

.......... .......... .......... .......... ClaI(1427) ..........

1381 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aggggatcga tccgtcctgt 1440

1381 tcgcgtagcg gaagatagcg gaagaactgc tcaagaagac tcccctagct aggcaggaca 1440

.......... NeoR/KanR ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aagtctgcag aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt 1500

1441 ttcagacgtc tttaactact agataatttg ttatttctac aggtgatttt accttcaaaa 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat 1560

1501 aggacagtat gaaacaattc ttcccactct tgtctcatgg atgtaaaact taccttccta 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tggagctacg ggggtggggg tggggtggga ttagataaat gcctgctctt tactgaaggc 1620

1561 acctcgatgc ccccaccccc accccaccct aatctattta cggacgagaa atgacttccg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tctttactat tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa 1680

1621 agaaatgata acgaaatact attacaaagt atcaacctat agtattaaat ttgttcgttt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 accaaattaa gggccagctc attcctccca ctcatgatct atagatctat agatctctcg 1740

1681 tggtttaatt cccggtcgag taaggagggt gagtactaga tatctagata tctagagagc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa 1800

1741 accctagtaa caaaaagaga actaagggtg aaacaccaag attcatgaca ccaaaggttt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat 1860

1801 acacagtcaa agtatcggac ttcttgctct agtcgtcgga gacaaggtgt atgtgaagta 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tctcagtatt gttttgccaa gttctaattc catcagaagc tgactctaga ggcctataac 1920

1861 agagtcataa caaaacggtt caagattaag gtagtcttcg actgagatct ccggatattg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... ......... BamHI(1955) ......... ..........

1921 ttcgtatagc atacattata cgaagttatg cgcaggatcc ccggttaacg ccttaattaa 1980

1921 aagcatatcg tatgtaatat gcttcaatac gcgtcctagg ggccaattgc ggaattaatt 1980

.......... loxP .......... .......... .......... ..........

XhoI(1984) NheI(1993) ......... EcoRI(2014) ......... ..........

1981 ctcctcgagc atgctagcgt cgatttaaat gcggaattct accgggtagg ggaggcgctt 2040

1981 gaggagctcg tacgatcgca gctaaattta cgccttaaga tggcccatcc cctccgcgaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tggcacttgg cgctacacaa 2100

2041 aagggttccg tcagacctcg tacgcgaaat cgtcggggcg accgtgaacc gcgatgtgtt 2100

.......... .......... MSCV_r_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtggcctctg gcctcgcaca cattccacat ccaccggtag cgccaaccgg ctccgttctt 2160

2101 caccggagac cggagcgtgt gtaaggtgta ggtggccatc gcggttggcc gaggcaagaa 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc 2220

2161 accaccgggg aagcgcggtg gaagatgagg aggggatcag tccttcaagg gggggcgggg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca 2280

2221 cgtcgagcgc agcacgtcct gcactgttta ccttcatcgt gcagagtgat cagagcacgt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca 2340

2281 ctacctgtcg tggcgactcg ttaccttcgc ccatccggaa accccgtcgc cggttatcgt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct 2400

2341 cgaaacgagg aagcgaaaga cccgagtctc cgacccttcc ccacccaggc ccccgcccga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 caggggcggg ctcaggggcg gggcgggcgc gaaggtcctc ccgaggcccg gcattctcgc 2460

2401 gtccccgccc gagtccccgc cccgcccgcg cttccaggag ggctccgggc cgtaagagcg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg 2520

2461 tgcgaagttt tcgcgtgcag acggcgcgac aagaggagaa ggagtagagg cccggaaagc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acctgcagcg acccgcttaa cagcgtcaac agcgtgccgc agatcttggt ggcgtgaaac 2580

2521 tggacgtcgc tgggcgaatt gtcgcagttg tcgcacggcg tctagaacca ccgcactttg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcccgcacct cttcggccag cgccttgtag aagcgcgtat ggcttcgtac ccctgccatc 2640

2581 agggcgtgga gaagccggtc gcggaacatc ttcgcgcata ccgaagcatg gggacggtag 2640

.......... .......... .......... .......... ORF3 ..........

.......... .......... .......... .......... .......... ..........

2641 aacacgcgtc tgcgttcgac caggctgcgc gttctcgcgg ccataacaac cgacgtacgg 2700

2641 ttgtgcgcag acgcaagctg gtccgacgcg caagagcgcc ggtattgttg gctgcatgcc 2700

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgttgcgccc tcgccggcaa caaaaagcca cggaagtccg cctggagcag aaaatgccca 2760

2701 gcaacgcggg agcggccgtt gtttttcggt gccttcaggc ggacctcgtc ttttacgggt 2760

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgctactgcg ggtttatata gacggtcccc acgggatggg gaaaaccacc accacgcaac 2820

2761 gcgatgacgc ccaaatatat ctgccagggg tgccctaccc cttttggtgg tggtgcgttg 2820

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgctggtggc cctgggttcg cgcgacgata tcgtctacgt acccgagccg atgacttact 2880

2821 acgaccaccg ggacccaagc gcgctgctat agcagatgca tgggctcggc tactgaatga 2880

.......... .......... ORF3 .......... .......... ..........

.......... .......... NruI(2910) .......... .......... ..........

2881 ggcgggtgtt gggggcttcc gagacaatcg cgaacatcta caccacacaa caccgcctcg 2940

2881 ccgcccacaa cccccgaagg ctctgttagc gcttgtagat gtggtgtgtt gtggcggagc 2940

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 accagggtga gatatcggcc ggggacgcgg cggtggtaat gacaagcgcc cagataacaa 3000

2941 tggtcccact ctatagccgg cccctgcgcc gccaccatta ctgttcgcgg gtctattgtt 3000

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgggcatgcc ttatgccgtg accgacgccg ttctggctcc tcatatcggg ggggaggctg 3060

3001 acccgtacgg aatacggcac tggctgcggc aagaccgagg agtatagccc cccctccgac 3060

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggagctcaca tgccccgccc ccggccctca ccctcatctt cgaccgccat cccatcgccg 3120

3061 cctcgagtgt acggggcggg ggccgggagt gggagtagaa gctggcggta gggtagcggc 3120

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccctcctgtg ctacccggcc gcgcgatacc ttatgggcag catgaccccc caggccgtgc 3180

3121 gggaggacac gatgggccgg cgcgctatgg aatacccgtc gtactggggg gtccggcacg 3180

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tggcgttcgt ggccctcatc ccgccgacct tgcccggcac aaacatcgtg ttgggggccc 3240

3181 accgcaagca ccgggagtag ggcggctgga acgggccgtg tttgtagcac aacccccggg 3240

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttccggagga cagacacatc gaccgcctgg ccaaacgcca gcgccccggc gagcggcttg 3300

3241 aaggcctcct gtctgtgtag ctggcggacc ggtttgcggt cgcggggccg ctcgccgaac 3300

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 acctggctat gctggccgcg attcgccgcg tttatgggct gcttgccaat acggtgcggt 3360

3301 tggaccgata cgaccggcgc taagcggcgc aaatacccga cgaacggtta tgccacgcca 3360

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atctgcaggg cggcgggtcg tggcgggagg attggggaca gctttcgggg gcggccgtgc 3420

3361 tagacgtccc gccgcccagc accgccctcc taacccctgt cgaaagcccc cgccggcacg 3420

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgccccaggg tgccgagccc cagagcaacg cgggcccacg accccatatc ggggacacgt 3480

3421 gcggggtccc acggctcggg gtctcgttgc gcccgggtgc tggggtatag cccctgtgca 3480

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tatttaccct gtttcgggcc cccgagttgc tggcccccaa cggcgacctg tataacgtgt 3540

3481 ataaatggga caaagcccgg gggctcaacg accgggggtt gccgctggac atattgcaca 3540

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttgcctgggc tttggacgtc ttggccaaac gcctccgtcc catgcatgtc tttatcctgg 3600

3541 aacggacccg aaacctgcag aaccggtttg cggaggcagg gtacgtacag aaataggacc 3600

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 attacgacca atcgcccgcc ggctgccggg acgccctgct gcaacttacc tccgggatgg 3660

3601 taatgctggt tagcgggcgg ccgacggccc tgcgggacga cgttgaatgg aggccctacc 3660

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tccagaccca cgtcaccacc ccaggctcca taccgacgat ctgcgacctg gcgcgcacgt 3720

3661 aggtctgggt gcagtggtgg ggtccgaggt atggctgcta gacgctggac cgcgcgtgca 3720

.......... .......... ORF3 .......... .......... ..........

SmaI(3726) .......... .......... .......... .......... ..........

3721 ttgcccggga gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 3780

3721 aacgggccct ctaccccctc cgattgactt tgtgccttcc tctgttatgg ccttccttgg 3780

.......... ORF3 .......... TK_PA_term .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca 3840

3781 gcgcgatact gccgttattt ttctgtctta ttttgcgtgc ccacaaccca gcaaacaagt 3840

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 3900

3841 atttgcgccc caagccaggg tcccgaccgt gagacagcta tggggtggct ctggggtaac 3900

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggaccaatac gcccgcgttt cttccttttc cccaccccaa cccccaagtt cgggtgaagg 3960

3901 cctggttatg cgggcgcaaa gaaggaaaag gggtggggtt gggggttcaa gcccacttcc 3960

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cccagggctc gcagccaacg tcggggcggc aagccctgcc atagccacgg gccccgtggg 4020

3961 gggtcccgag cgtcggttgc agccccgccg ttcgggacgg tatcggtgcc cggggcaccc 4020

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc 4080

4021 aatccctgcc ccagggggta ccccttacca aataccaagc acccccaata ataaaacccg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt tttggatggc 4140

4081 caacgcaccc cagtccaggt gctgacctga ctcgtctgtc tgggtaccaa aaacctaccg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac 4200

4141 gacccgtacc tggcgtacat gaccgcgctg tgcttgtggc ccgcagacac cgacggtttg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acccccgacc cccaaaaacc accgcgcgga tttctggcgc cgccggacga actaaacctg 4260

4201 tgggggctgg gggtttttgg tggcgcgcct aaagaccgcg gcggcctgct tgatttggac 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 actacggcat ctctgcccct tcttcgctgg tacgaggagc gcttttgttt tgtattggtc 4320

4261 tgatgccgta gagacgggga agaagcgacc atgctcctcg cgaaaacaaa acataaccag 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 accacggccg agtttccgcg ggaccccggc caggacctgc agaaattgat gatctattaa 4380

4321 tggtgccggc tcaaaggcgc cctggggccg gtcctggacg tctttaacta ctagataatt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga 4440

4381 tgttatttct acaggtgatt ttaccttcaa aaaggacagt atgaaacaat tcttcccact 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg 4500

4441 cttgtctcat ggatgtaaaa cttaccttcc taacctcgat gcccccaccc ccaccccacc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gattagataa atgcctgctc tttactgaag gctctttact attgctttat gataatgttt 4560

4501 ctaatctatt tacggacgag aaatgacttc cgagaaatga taacgaaata ctattacaaa 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc 4620

4561 gtatcaacct atagtattaa atttgttcgt tttggtttaa ttcccggtcg agtaaggagg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc 4680

4621 gtgagtacta gatatctaga tatctagaga gcaccctagt aacaaaaaga gaactaaggg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga 4740

4681 tgaaacacca agattcatga caccaaaggt ttacacagtc aaagtatcgg acttcttgct 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat 4800

4741 ctagtcgtcg gagacaaggt gtatgtgaag taagagtcat aacaaaacgg ttcaagatta 4800

.......... .......... .......... .......... .......... ..........

HindIII(4809) ...... .......... .......... .......... ..........

4801 tccatcagaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 4860

4801 aggtagtctt cgaaccgtga ccggcagcaa aatgttgcag cactgaccct tttgggaccg 4860

.......... ......... M13_f20_pri M13_pUC_f lacZ_a .........

.......... .......... .......... .......... .......... ..........

4861 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 4920

4861 caatgggttg aattagcgga acgtcgtgta gggggaaagc ggtcgaccgc attatcgctt 4920

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg 4980

4921 ctccgggcgt ggctagcggg aagggttgtc aacgcgtcgg acttaccgct taccgcggac 4980

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... NdeI(5026) ..........

4981 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc 5040

4981 tacgccataa aagaggaatg cgtagacacg ccataaagtg tggcgtatac cacgtgagag 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 5100

5041 tcatgttaga cgagactacg gcgtatcaat tcggtcgggg ctgtgggcgg ttgtgggcga 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 5160

5101 ctgcgcggga ctgcccgaac agacgagggc cgtaggcgaa tgtctgttcg acactggcag 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 5220

5161 aggccctcga cgtacacagt ctccaaaagt ggcagtagtg gctttgcgcg ctctgctttc 5220

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 5280

5221 ccggagcact atgcggataa aaatatccaa ttacagtact attattacca aagaatctgc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 5340

5281 agtccaccgt gaaaagcccc tttacacgcg ccttggggat aaacaaataa aaagatttat 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 5400

5341 gtaagtttat acataggcga gtactctgtt attgggacta tttacgaagt tattataact 5400

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 5460

5401 ttttccttct catactcata agttgtaaag gcacagcggg aataagggaa aaaacgccgt 5460

.......... .......... ....... ORF1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

5461 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 5520

5461 aaaacggaag gacaaaaacg agtgggtctt tgcgaccact ttcattttct acgacttcta 5520

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 5580

5521 gtcaacccac gtgctcaccc aatgtagctt gacctagagt tgtcgccatt ctaggaactc 5580

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 5640

5581 tcaaaagcgg ggcttcttgc aaaaggttac tactcgtgaa aatttcaaga cgatacaccg 5640

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 5700

5641 cgccataata gggcataact gcggcccgtt ctcgttgagc cagcggcgta tgtgataaga 5700

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 5760

5701 gtcttactga accaactcat gagtggtcag tgtcttttcg tagaatgcct accgtactgt 5760

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 5820

5761 cattctctta atacgtcacg acggtattgg tactcactat tgtgacgccg gttgaatgaa 5820

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 5880

5821 gactgttgct agcctcctgg cttcctcgat tggcgaaaaa acgtgttgta ccccctagta 5880

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 5940

5881 cattgagcgg aactagcaac ccttggcctc gacttacttc ggtatggttt gctgctcgca 5940

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 6000

5941 ctgtggtgct acggacatcg ttaccgttgt tgcaacgcgt ttgataattg accgcttgat 6000

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 6060

6001 gaatgagatc gaagggccgt tgttaattat ctgacctacc tccgcctatt tcaacgtcct 6060

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 6120

6061 ggtgaagacg cgagccggga aggccgaccg accaaataac gactatttag acctcggcca 6120

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 6180

6121 ctcgcaccca gagcgccata gtaacgtcgt gaccccggtc taccattcgg gagggcatag 6180

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 6240

6181 catcaataga tgtgctgccc ctcagtccgt tgatacctac ttgctttatc tgtctagcga 6240

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 6300

6241 ctctatccac ggagtgacta attcgtaacc attgacagtc tggttcaaat gagtatatat 6300

....... ORF1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 6360

6301 gaaatctaac taaattttga agtaaaaatt aaattttcct agatccactt ctaggaaaaa 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 6420

6361 ctattagagt actggtttta gggaattgca ctcaaaagca aggtgactcg cagtctgggg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 6480

6421 catcttttct agtttcctag aagaactcta ggaaaaaaag acgcgcatta gacgacgaac 6480

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

6481 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 6540

6481 gtttgttttt ttggtggcga tggtcgccac caaacaaacg gcctagttct cgatggttga 6540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 6600

6541 gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg gtttatgaca ggaagatcac 6600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 6660

6601 atcggcatca atccggtggt gaagttcttg agacatcgtg gcggatgtat ggagcgagac 6660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 6720

6661 gattaggaca atggtcaccg acgacggtca ccgctattca gcacagaatg gcccaacctg 6720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 6780

6721 agttctgcta tcaatggcct attccgcgtc gccagcccga cttgcccccc aagcacgtgt 6780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 6840

6781 gtcgggtcga acctcgcttg ctggatgtgg cttgactcta tggatgtcgc actcgatact 6840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 6900

6841 ctttcgcggt gcgaagggct tccctctttc cgcctgtcca taggccattc gccgtcccag 6900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 6960

6901 ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc ggaccataga aatatcagga 6960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 7020

6961 cagcccaaag cggtggagac tgaactcgca gctaaaaaca ctacgagcag tccccccgcc 7020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7021 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 7080

7021 tcggatacct ttttgcggtc gttgcgccgg aaaaatgcca aggaccggaa aacgaccgga 7080

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 7140

7081 aaacgagtgt acaagaaagg acgcaatagg ggactaagac acctattggc ataatggcgg 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 7200

7141 aaactcactc gactatggcg agcggcgtcg gcttgctggc tcgcgtcgct cagtcactcg 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 7260

7201 ctccttcgcc ttctcgcggg ttatgcgttt ggcggagagg ggcgcgcaac cggctaagta 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 7320

7261 attacgtcga ccgtgctgtc caaagggctg acctttcgcc cgtcactcgc gttgcgttaa 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 7380

7321 ttacactcaa tcgagtgagt aatccgtggg gtccgaaatg tgaaatacga aggccgagca 7380

.......... .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

7381 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat 7440

7381 tacaacacac cttaacactc gcctattgtt aaagtgtgtc ctttgtcgat actggtacta 7440

.......... .......... M13_pUC_r .......... M13_r ..........

.......... .......... .......... .......... .......... ..........

7441 tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt 7500

7441 atgcggttcg agctttaatt gggagtgatt tcccttgttt tcgacctcga ggtggcgcca 7500

.......... .......... T3_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 g......... .......... .......... .......... .......... .......... 7501

7501 c......... .......... .......... .......... .......... .......... 7501

.......... .......... .......... .......... .......... ..........