qR=qR=!$ID FJ477110; SV 1; circular; other DNA; STD; SYN; 4163 BP. AC FJ477110; DT 30-DEC-2008 (Rel. 98, Created) DT 30-DEC-2008 (Rel. 98, Last updated, Version 1) DE Reporter vector p6851, complete sequence. KW . OS Reporter vector p6851 OC other sequences; artificial sequences; vectors. RN [1] RP 1-4163 RA Cambray G., Mazel D.; RT "plasmid p6851, integron cassette excision reporter, positively RT selectable"; RL Unpublished. RN [2] RP 1-4163 RA Cambray G., Mazel D.; RT ; RL Submitted (19-NOV-2008) to the EMBL/GenBank/DDBJ databases. RL Plasticite du genome bacterien, Institut Pasteur, 25 rue du Dr Roux, Paris RL 75724, France FH Key Location/Qualifiers FT source 1..4163 FT /organism="Reporter vector p6851" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:585036" FT gene 1..93 FT /gene="lacZalpha" FT CDS 1..93 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZalpha" FT /product="b-galactosidase Nterm" FT /protein_id="ACL01110.1" FT /translation="MITNSVITIHSSRRRFAARLNSSVRWDPLP" FT misc_recomb 20..73 FT /gene="lacZalpha" FT /note="attC aadA7" FT misc_feature 71..1125 FT /note="IntI mediated recombination deletes sequence and FT allows translation of aac6'-Ib, which confers selectable FT resistance to tobramycin" FT gene complement(99..758) FT /gene="cat" FT CDS complement(99..758) FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="Cat" FT /note="chloramphenicol acetyl transferase" FT /protein_id="ACL01111.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILTRLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT misc_recomb 1006..1685 FT /note="attC VCR" FT gene 1167..1685 FT /gene="aac(6')-Ib*" FT CDS 1167..1685 FT /codon_start=1 FT /transl_table=11 FT /gene="aac(6')-Ib*" FT /product="Aac(6')-Ib*" FT /protein_id="ACL01112.1" FT /translation="MTEHDLAMLYEWLNRSHIVEWWGGEEARPTLADVQEQYLPSVLAQ FT ESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQLLANASQLGKGLGTK FT LVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTPDGPAVYMVQTRQ FT AFERTRSDA" FT gene complement(1944..2738) FT /gene="aac(6')-Ib*" FT CDS complement(1944..2738) FT /codon_start=1 FT /transl_table=11 FT /gene="aac(6')-Ib*" FT /product="Aac(6')-Ib*" FT /protein_id="ACL01113.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" SQ Sequence 4163 BP; 994 A; 1113 C; 1079 G; 977 T; 0 other; atgattacga attcggttat aacaattcat tcaagccgac gccgcttcgc ggcgcggctt 60 aattcaagcg ttagatggga tccactgcct taaaaaaatt acgccccgcc ctgccactca 120 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 180 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 240 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 300 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 360 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 420 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 480 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga 540 aattccggat gagcattcat caggcgggta agaatgtgaa taaaggccgg ataaaacttg 600 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 660 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 720 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 780 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 840 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg 900 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt 960 tattcgagcc cgcctaatga gcgggctttt ttttagatct ggttataaca aacgcctcaa 1020 gagggactgt caacgcgtgg cgtttccagt cccattgagc cgcggtggtt gctgttgttg 1080 tgtttgagtt tagtggtagt gcgttgtcag ccccttaggc gggcgttatt ctgaccaaca 1140 gcaacgattc cgtcacactg cgcctcatga ctgagcatga ccttgcgatg ctctatgagt 1200 ggctaaatcg atctcatatc gtcgagtggt ggggcggaga agaagcacgc ccgacacttg 1260 ctgacgtaca ggaacagtac ttgccaagcg ttttagcgca agagtccgtc actccataca 1320 ttgcaatgct gaatggagag ccgattgggt atgcccagtc gtacgttgct cttggaagcg 1380 gggacggatg gtgggaagaa gaaaccgatc caggagtacg cggaatagac cagttactgg 1440 cgaatgcatc acaactgggc aaaggcttgg gaaccaagct ggttcgagct ctggttgagt 1500 tgctgttcaa tgatcccgag gtcaccaaga tccaaacgga cccgtcgccg agcaacttgc 1560 gagcgatccg atgctacgag aaagcggggt ttgagaggca aggtaccgta accaccccag 1620 atggtccagc cgtgtacatg gttcaaacac gccaggcatt cgagcgaaca cgcagtgatg 1680 cctaataaca tatgggatgt tcggtaagct tgtccggcgg tgcttttgcc gttacgcacc 1740 accccgtcag tagctgaaca ggagggacag ggggtgggcg aagaactcca gcatgagatc 1800 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc 1860 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt 1920 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg 1980 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg 2040 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca 2100 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc 2160 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatccg cgccttgagc 2220 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg 2280 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg 2340 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat 2400 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat 2460 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc 2520 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgga gttcattcag ggcaccggac 2580 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca 2640 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg 2700 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc 2760 tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag 2820 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg 2880 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc 2940 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc 3000 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc 3060 ttgcgccctg agtgcttgcg gcagcgtgaa gctgtgccta gaaatatttt atctgattaa 3120 taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa 3180 aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct ttgaaccgag 3240 gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg 3300 cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc cagtggtgct 3360 tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3420 gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg 3480 agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac cggtaaaccg 3540 aaaggcagga acaggagagc gcacgaggga gccgccaggg gaaacgcctg gtatctttat 3600 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg 3660 gggcggagcc tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct 3720 tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc 3780 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat 3840 tctgctgacg caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc 3900 ctcatcagtg ccaacatagt aagccagtat atacactccg ctagcgccca atacgcaaac 3960 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 4020 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 4080 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 4140 ttcacacagg aaacagctat gac 4163 Cp6851"Times New Roman qR='EcoRI(10),BamHI(79),ScaI(128),NcoI(240) ArialOCrh^l ^ qR=PvuII ArialC(088j qR=-BglII(996),MluI(1034),Bsu36I(1115),ClaI(1209) Arial CQR[RdQd9i qR=ScaI(1279),SacI(1491) ArialCDMVV qR=#KpnI(1607),NdeI(1691),HindIII(1707) ArialC%%6I qR=NcoI Arial~CO;kQ^`*`^l qR=-PvuII(2504),EagI(2700),BclI(2765),BglII(2770) Arial Cj qR=PvuII Arial0 CN qR=NheI(3941),PvuII(3998) ArialeC~upd|qR=CC] lacZalpha"Times New RomanqR=Cccat"Times New RomanqR=CɆCtrpA_terminator"Times New RomanqR=CɆC aac(6')-Ib*"Times New RomanqR=CCe} ORF 2"Times New RomanqR=CC  aac(6')-Ib*"Times New RomanqR=C < NEOKAN_promoter"Times New RomanqR=CC lac_promoter"Times New RomanqR=CC 6M13_pUC_rev_primer"Times New Roman qR=test"Times New RomanC[q[gN]AN]