Sequence of p6851

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Sequence of p6851

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Sequence of p6851: 4163 BP; 994 A; 1113 C; 1079 G; 977 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atgattacga attcggttat aacaattcat tcaagccgac gccgcttcgc ggcgcggctt 60

1 tactaatgct taagccaata ttgttaagta agttcggctg cggcgaagcg ccgcgccgaa 60

.......... .......... lacZalpha .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aattcaagcg ttagatggga tccactgcct taaaaaaatt acgccccgcc ctgccactca 120

61 ttaagttcgc aatctaccct aggtgacgga atttttttaa tgcggggcgg gacggtgagt 120

.......... lacZalpha .......... .......... cat ..........

.......... .......... .......... .......... .......... ..........

121 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 180

121 agcgtcatga caacattaag taattcgtaa gacggctgta ccttcggtag tgtttgccgt 180

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... NcoI(240)

181 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 240

181 actacttgga cttagcggtc gccgtagtcg tggaacagcg gaacgcatat tataaacggg 240

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 300

241 taccactttt gcccccgctt cttcaacagg tataaccggt gcaaatttag ttttgaccac 300

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 360

301 tttgagtggg tccctaaccg actctgcttt ttgtataaga gttatttggg aaatcccttt 360

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 420

361 atccggtcca aaagtggcat tgtgcggtgt agaacgctta tatacacatc tttgacggcc 420

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 480

421 tttagcagca ccataagtga ggtctcgcta cttttgcaaa gtcaaacgag taccttttgc 480

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga 540

481 cacattgttc ccacttgtga tagggtatag tggtcgagtg gcagaaagta acggtatgct 540

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aattccggat gagcattcat caggcgggta agaatgtgaa taaaggccgg ataaaacttg 600

541 ttaaggccta ctcgtaagta gtccgcccat tcttacactt atttccggcc tattttgaac 600

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... PvuII(645) ..........

601 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 660

601 acgaataaaa agaaatgcca gaaatttttc cggcattata ggtcgacttg ccagaccaat 660

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 720

661 atccatgtaa ctcgttgact gactttacgg agttttacaa gaaatgctac ggtaacccta 720

.......... .......... cat .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 780

721 tatagttgcc accatatagg tcactaaaaa aagaggtaaa atcgaaggaa tcgaggactt 780

.......... cat .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 840

781 ttagagctat tgagtttttt atgcgggcca tcactagaat aaagtaatac cactttcaac 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg 900

841 cttggagaat gcacggctag ttgcagagta aaagcggttt tcaaccgggt cccgaagggc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt 960

901 catagttgtc cctgtggtcc taaataaata agacgcttca ctagaaggca gtgtccataa 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tattcgagcc cgcctaatga gcgggctttt ttttagatct ggttataaca aacgcctcaa 1020

961 ataagctcgg gcggattact cgcccgaaaa aaaatctaga ccaatattgt ttgcggagtt 1020

....... trpA_terminator ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gagggactgt caacgcgtgg cgtttccagt cccattgagc cgcggtggtt gctgttgttg 1080

1021 ctccctgaca gttgcgcacc gcaaaggtca gggtaactcg gcgccaccaa cgacaacaac 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgtttgagtt tagtggtagt gcgttgtcag ccccttaggc gggcgttatt ctgaccaaca 1140

1081 acaaactcaa atcaccatca cgcaacagtc ggggaatccg cccgcaataa gactggttgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gcaacgattc cgtcacactg cgcctcatga ctgagcatga ccttgcgatg ctctatgagt 1200

1141 cgttgctaag gcagtgtgac gcggagtact gactcgtact ggaacgctac gagatactca 1200

.......... .......... .......... ......... aac(6')-Ib* .........

ClaI(1209) .......... .......... .......... .......... ..........

1201 ggctaaatcg atctcatatc gtcgagtggt ggggcggaga agaagcacgc ccgacacttg 1260

1201 ccgatttagc tagagtatag cagctcacca ccccgcctct tcttcgtgcg ggctgtgaac 1260

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctgacgtaca ggaacagtac ttgccaagcg ttttagcgca agagtccgtc actccataca 1320

1261 gactgcatgt ccttgtcatg aacggttcgc aaaatcgcgt tctcaggcag tgaggtatgt 1320

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttgcaatgct gaatggagag ccgattgggt atgcccagtc gtacgttgct cttggaagcg 1380

1321 aacgttacga cttacctctc ggctaaccca tacgggtcag catgcaacga gaaccttcgc 1380

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gggacggatg gtgggaagaa gaaaccgatc caggagtacg cggaatagac cagttactgg 1440

1381 ccctgcctac cacccttctt ctttggctag gtcctcatgc gccttatctg gtcaatgacc 1440

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... SacI(1491)

1441 cgaatgcatc acaactgggc aaaggcttgg gaaccaagct ggttcgagct ctggttgagt 1500

1441 gcttacgtag tgttgacccg tttccgaacc cttggttcga ccaagctcga gaccaactca 1500

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgctgttcaa tgatcccgag gtcaccaaga tccaaacgga cccgtcgccg agcaacttgc 1560

1501 acgacaagtt actagggctc cagtggttct aggtttgcct gggcagcggc tcgttgaacg 1560

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gagcgatccg atgctacgag aaagcggggt ttgagaggca aggtaccgta accaccccag 1620

1561 ctcgctaggc tacgatgctc tttcgcccca aactctccgt tccatggcat tggtggggtc 1620

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atggtccagc cgtgtacatg gttcaaacac gccaggcatt cgagcgaaca cgcagtgatg 1680

1621 taccaggtcg gcacatgtac caagtttgtg cggtccgtaa gctcgcttgt gcgtcactac 1680

.......... ......... aac(6')-Ib* ......... .......... ..........

........ NdeI(1691) HindIII(1707) ........ .......... ..........

1681 cctaataaca tatgggatgt tcggtaagct tgtccggcgg tgcttttgcc gttacgcacc 1740

1681 ggattattgt ataccctaca agccattcga acaggccgcc acgaaaacgg caatgcgtgg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 accccgtcag tagctgaaca ggagggacag ggggtgggcg aagaactcca gcatgagatc 1800

1741 tggggcagtc atcgacttgt cctccctgtc ccccacccgc ttcttgaggt cgtactctag 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc 1860

1801 gggcgcgacc tcctagtagg tcggccgcag ggccttttgc taaggcttcg ggttggaaag 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt 1920

1861 tatcttccgc cgccacctta gctttagagc actaccgtcc aacccgcagc gaaccagcca 1920

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

1921 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg 1980

1921 gtaaagcttg gggtctcagg gcgagtcttc ttgagcagtt cttccgctat cttccgctac 1980

.......... .......... ORF 2 ......... aac(6')-Ib* .........

.......... .......... .......... .......... .......... ..........

1981 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg 2040

1981 gcgacgctta gccctcgccg ctatggcatt tcgtgctcct tcgccagtcg ggtaagcggc 2040

.......... ...... ORF 2 aac(6')-Ib* ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca 2100

2041 ggttcgagaa gtcgttatag tgcccatcgg ttgcgataca ggactatcgc caggcggtgt 2100

.......... ...... ORF 2 aac(6')-Ib* ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc 2160

2101 gggtcggccg gtgtcagcta cttaggtctt ttcgccggta aaaggtggta ctataagccg 2160

.......... ...... ORF 2 aac(6')-Ib* ...... .......... ..........

.......... NcoI(2174) .......... .......... .......... ..........

2161 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatccg cgccttgagc 2220

2161 ttcgtccgta gcggtaccca gtgctgctct aggagcggca gcccgtaggc gcggaactcg 2220

.......... ...... ORF 2 aac(6')-Ib* ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg 2280

2221 gaccgcttgt caagccgacc gcgctcgggg actacgagaa gcaggtctag taggactagc 2280

.......... ...... ORF 2 aac(6')-Ib* ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg 2340

2281 tgttctggcc gaaggtaggc tcatgcacga gcgagctacg ctacaaagcg aaccaccagc 2340

.......... ...... ORF 2 aac(6')-Ib* ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat 2400

2341 ttacccgtcc atcggcctag ttcgcatacg tcggcggcgt aacgtagtcg gtactaccta 2400

.......... ...... ORF 2 aac(6')-Ib* ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat 2460

2401 tgaaagagcc gtcctcgttc cactctactg tcctctagga cggggccgtg aagcgggtta 2460

......... ORF 2 aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc 2520

2461 tcgtcggtca gggaagggcg aagtcactgt tgcagctcgt gtcgacgcgt tccttgcggg 2520

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgga gttcattcag ggcaccggac 2580

2521 cagcaccggt cggtgctatc ggcgcgacgg agcagaacct caagtaagtc ccgtggcctg 2580

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca 2640

2581 tccagccaga actgtttttc ttggcccgcg gggacgcgac tgtcggcctt gtgccgccgt 2640

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg 2700

2641 agtctcgtcg gctaacagac aacacgggtc agtatcggct tatcggagag gtgggttcgc 2700

.......... ......... aac(6')-Ib* ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc 2760

2701 cggcctcttg gacgcacgtt aggtagaaca agttagtacg ctttgctagg agtaggacag 2760

......... aac(6')-Ib* ......... .......... .......... ..........

BclI(2765),BglII(2770) ........ .......... .......... ..........

2761 tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag 2820

2761 agaactagtc tagaactagg ggacgcggta gtctaggaac cgccgttctt tcggtaggtc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... PvuII(2864) .........

2821 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg 2880

2821 aaatgaaacg tcccgaaggg ttggaatggt ctcccgcggg gtcgaccgtt aaggccaagc 2880

.......... .......... ....... NEOKAN_promoter ....... ..........

.......... .......... .......... .......... .......... ..........

2881 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc 2940

2881 gaacgacagg tattttggcg ggtcagatcg atagcggtac attcgggtga cgttcgatgg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc 3000

2941 acgaaagaga aacgcgaacg caaaagggaa caggtctatc gggtcatcga ctgtaagtag 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc 3060

3001 gccccagtcg tggcaaagac gcctgaccga aagatgcaca aggcgaagga aatcgtcggg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttgcgccctg agtgcttgcg gcagcgtgaa gctgtgccta gaaatatttt atctgattaa 3120

3061 aacgcgggac tcacgaacgc cgtcgcactt cgacacggat ctttataaaa tagactaatt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa 3180

3121 attctactag aagaactcta gcaaaaccag acgcgcatta gagaacgaga cttttgcttt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct ttgaaccgag 3240

3181 tttggcggaa cgtcccgcca aaaagcttcc aagagactcg atggttgaga aacttggctc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg 3300

3241 cattgaccga acctcctcgc gtcagtggtt ttgaacagga aagtcaaatc ggaattggcc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc cagtggtgct 3360

3301 gcgtactgaa gttctgattg aggagattta gttaatggtc accgacgacg gtcaccacga 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3420

3361 aaacgtacag aaaggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg 3480

3421 ctgacttgcc ccccaagcac gtatgtcagg tcgaacctcg cttgacggat gggccttgac 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac cggtaaaccg 3540

3481 tcacagtccg caccttactc tgtttgcgcc ggtattgtcg ccttactgtg gccatttggc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aaaggcagga acaggagagc gcacgaggga gccgccaggg gaaacgcctg gtatctttat 3600

3541 tttccgtcct tgtcctctcg cgtgctccct cggcggtccc ctttgcggac catagaaata 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg 3660

3601 tcaggacagc ccaaagcggt ggtgactaaa ctcgcagtct aaagcactac gaacagtccc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gggcggagcc tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct 3720

3661 cccgcctcgg ataccttttt gccgaaacgg cgccgggaga gtgaagggac aattcataga 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc 3780

3721 aggaccgtag aaggtccttt agaggcgggg caagcattcg gtaaaggcga gcggcgtcag 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat 3840

3781 cttgctggct cgcatcgctc agtcactcgc tccttcgcct tatataggac atagtgtata 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tctgctgacg caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc 3900

3841 agacgactgc gtggccacgt cggaaaaaag aggacggtgt acttcgtgaa gtgactgtgg 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctcatcagtg ccaacatagt aagccagtat atacactccg ctagcgccca atacgcaaac 3960

3901 gagtagtcac ggttgtatca ttcggtcata tatgtgaggc gatcgcgggt tatgcgtttg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... ......... PvuII(3998) ......... ..........

3961 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 4020

3961 gcggagaggg gcgcgcaacc ggctaagtaa ttacgtcgac cgtgctgtcc aaagggctga 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 4080

4021 cctttcgccc gtcactcgcg ttgcgttaat tacactcaat cgagtgagta atccgtgggg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 4140

4081 tccgaaatgt gaaatacgaa ggccgagcat acaacacacc ttaacactcg cctattgtta 4140

......... lac_promoter ......... .......... ...... M13_pUC_rev_primer

.......... .......... .......... .......... .......... ..........

4141 ttcacacagg aaacagctat gac....... .......... .......... .......... 4163

4141 aagtgtgtcc tttgtcgata ctg....... .......... .......... .......... 4163

M13_pUC_rev_primer .. .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-4163 Reference author: Cambray G., Mazel D.; Reference title: "plasmid p6851, integron cassette excision reporter, positively Reference title: selectable"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-4163 Reference author: Cambray G., Mazel D.; Reference title: ; Reference location: Submitted (19-NOV-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Plasticite du genome bacterien, Institut Pasteur, 25 rue du Dr Roux, Paris Reference location: 75724, France