Sequence of p70-S6-kinase

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Sequence of p70-S6-kinase

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Sequence of p70 alpha1 S6 kinase : 6800 BP; 1771 A; 1602 C; 1784 G; 1643 T; 0 other;

.......... .......... StuI(30) .......... .......... ..........

1 ttgtttccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg 60

1 aacaaaggtt cgaaaaacgt tttcggatcc ggaggttttt tcggaggagt gatgaagacc 60

.......... .......... .......... .......... SV40_pro ..........

.......... .......... .......... .......... .......... ..........

61 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca 120

61 ttatcgagtc tccggctccg ccggagccgg agacgtattt atttttttta atcagtcggt 120

.......... ...... SV40pro_F SV40_pro SV40_pro SV4_origin ......

.......... .......... .......... .......... .......... ..........

121 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc 180

121 accccgcctc ttacccgcct tgacccgcct caatccccgc cctacccgcc tcaatccccg 180

.......... . SV40_pro SV40_pro SV4_origin SV40_en . ..........

.......... .......... .......... .......... .......... ..........

181 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag 240

181 ccctgatacc aacgactgat taactctacg tacgaaacgt atgaagacgg acgacccctc 240

....... SV40_pro SV40_pro SV40_en ....... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg 300

241 ggacccctga aaggtgtgga ccaacgactg attaactcta cgtacgaaac gtatgaagac 300

.......... ....... SV40_pro SV40_en ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca caggatccgg 360

301 ggacgacccc tcggacccct gaaaggtgtg ggattgactg tgtgtaaggt gtcctaggcc 360

......... SV40_pro SV40_en pBABE_3_pri ORF2 .........

.......... .......... .......... .......... .......... ..........

361 tcgcgcgaat ttcgagcggt gttccgcggt cctcctcgta tagaaactcg gaccactctg 420

361 agcgcgctta aagctcgcca caaggcgcca ggaggagcat atctttgagc ctggtgagac 420

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agacgaaggc tcgcgtccag gccagcacga aggaggctaa gtgggagggg tagcggtcgt 480

421 tctgcttccg agcgcaggtc cggtcgtgct tcctccgatt caccctcccc atcgccagca 480

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgtccactag ggggtccact cgctccagtg gtgtgaagac acatgtcgcc ctcttcggca 540

481 acaggtgatc ccccaggtga gcgaggtcac cacacttctg tgtacagcgg gagaagccgt 540

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tcaaggaagg tgattggttt ataggtgtag gccacgtgac cgggtgttcc tgaagggggg 600

541 agttccttcc actaaccaaa tatccacatc cggtgcactg gcccacaagg acttcccccc 600

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ctataaaagg gggtgggggc gcgttcgtcc tcactctctt ccgcatcgct gtctgcgagg 660

601 gatattttcc cccacccccg cgcaagcagg agtgagagaa ggcgtagcga cagacgctcc 660

.......... .......... ORF2 .......... .......... synth_int

.......... .......... .......... .......... .......... ..........

661 gccagctgtt gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga 720

661 cggtcgacaa cccgagcgcc aactcctgtt tgagaagcgc cagaaaggtc atgagaacct 720

.......... ........ ORF2 synth_int ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcggaaaccc gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca 780

721 agcctttggg cagccggagg cttgccatga ggcggtggct ccctggactc gctcaggcgt 780

.......... ........ ORF2 synth_int ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 tcgaccggat cggaaaacct ctcgactgtt ggggtgagta ctccctctca aaagcgggca 840

781 agctggccta gccttttgga gagctgacaa ccccactcat gagggagagt tttcgcccgt 840

.......... ........ ORF2 synth_int ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc 900

841 actgaagacg cgattctaac agtcaaaggt ttttgctcct cctaaactat aagtggaccg 900

.......... ........ ORF2 synth_int ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc agaaaagaca atctttttgt 960

901 ggcgccacta cggaaactcc caccggcgca ggtagaccag tcttttctgt tagaaaaaca 960

.......... ........ ORF2 synth_int ........ .......... ..........

.......... .......... .......... MscI(995) .......... ..........

961 tgtcaagctt gaggtgtggc aggcttgaga tctggccata cacttgagtg acaatgacat 1020

961 acagttcgaa ctccacaccg tccgaactct agaccggtat gtgaactcac tgttactgta 1020

.......... ........ ORF2 synth_int ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcagg cgagcctgaa 1080

1021 ggtgaaacgg aaagagaggt gtccacaggt gagggtccag gttgacgtcc gctcggactt 1080

.......... ........ ORF2 synth_int ........ .......... ..........

NotI(1085) .......... .......... .......... ApaI(1130) ..........

1081 ttcgcggccg ccaccatgta cccatacgat gttccagatt acgctgggcc catgaggcga 1140

1081 aagcgccggc ggtggtacat gggtatgcta caaggtctaa tgcgacccgg gtactccgct 1140

.......... ...... ORF2 Orf frame 1 HA HA ...... ..........

.......... .......... .......... .......... .......... ..........

1141 cgacggaggc gggacggctt ttacccagcg cctgacttcc gacacaggga agctgaggac 1200

1141 gctgcctccg ccctgccgaa aatgggtcgc ggactgaagg ctgtgtccct tcgactcctg 1200

.......... ....... ORF2 Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atggcaggag tgtttgacat agacctggac cagccagagg atgcaggctc tgaggatgag 1260

1201 taccgtcctc acaaactgta tctggacctg gtcggtctcc tacgtccgag actcctactc 1260

.......... ....... ORF2 Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctggaggagg ggggtcagtt aaatgaaagc atggaccatg ggggagttgg accatatgaa 1320

1261 gacctcctcc ccccagtcaa tttactttcg tacctggtac cccctcaacc tggtatactt 1320

.......... ....... ORF2 Orf frame 1 ....... .......... ..........

.......... .......... .......... SpeI(1360) .......... ..........

1321 cttggcatgg aacattgtga gaaatttgaa atctcagaaa ctagtgtgaa cagagggcca 1380

1321 gaaccgtacc ttgtaacact ctttaaactt tagagtcttt gatcacactt gtctcccggt 1380

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gaaaaaatca gaccagaatg ttttgagcta cttcgggtac ttggtaaagg gggctatgga 1440

1381 cttttttagt ctggtcttac aaaactcgat gaagcccatg aaccatttcc cccgatacct 1440

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaggtttttc aagtacgaaa agtaacagga gcaaatactg ggaagatatt tgccatgaag 1500

1441 ttccaaaaag ttcatgcttt tcattgtcct cgtttatgac ccttctataa acggtacttc 1500

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtgcttaaaa aggcaatgat agtaagaaat gctaaagata cagctcatac aaaagcagag 1560

1501 cacgaatttt tccgttacta tcattcttta cgatttctat gtcgagtatg ttttcgtctc 1560

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cggaatattc tggaggaagt aaagcatccc ttcattgtgg atttaattta tgcctttcag 1620

1561 gccttataag acctccttca tttcgtaggg aagtaacacc taaattaaat acggaaagtc 1620

.......... ......... Orf frame 1 ......... .......... ..........

AgeI(1621) .......... .......... .......... .......... ..........

1621 accggtggaa aactctacct catccttgag tatctcagtg gaggagaact atttatgcag 1680

1621 tggccacctt ttgagatgga gtaggaactc atagagtcac ctcctcttga taaatacgtc 1680

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttagaaagag aggggatatt catggaagat acagcttgct tttacttggc tgaaatctcc 1740

1681 aatctttctc tcccctataa gtaccttcta tgtcgaacga aaatgaaccg actttagagg 1740

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 atggctttgg ggcatttaca tcaaaagggg attatctaca gagacctgaa gccggagaac 1800

1741 taccgaaacc ccgtaaatgt agttttcccc taatagatgt ctctggactt cggcctcttg 1800

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atcatgctta atcaccaagg tcacgtgaag ctgacagact ttggactatg caaagaatct 1860

1801 tagtacgaat tagtggttcc agtgcacttc gactgtctga aacctgatac gtttcttaga 1860

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 attcatgatg gaacagtcac gcacacattt tgtggaacaa tagaatacat ggcccctgaa 1920

1861 taagtactac cttgtcagtg cgtgtgtaaa acaccttgtt atcttatgta ccggggactt 1920

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atcttgatga gaagcggcca caaccgtgct gtggattggt ggagtctggg agcattaatg 1980

1921 tagaactact cttcgccggt gttggcacga cacctaacca cctcagaccc tcgtaattac 1980

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tatgacatgc tgactggagc acctccattc actggggaga atagaaagaa gacaattgac 2040

1981 atactgtacg actgacctcg tggaggtaag tgacccctct tatctttctt ctgttaactg 2040

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... XhoI(2090) ..........

2041 aaaatcctca aatgtaaact taatttgcct ccctacctca cacaagaagc tcgagatctg 2100

2041 ttttaggagt ttacatttga attaaacgga gggatggagt gtgttcttcg agctctagac 2100

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cttaaaaagc tgctgaagag aaatgctgct tctcgtcttg gagctggccc tggggatgct 2160

2101 gaatttttcg acgacttctc tttacgacga agagcagaac ctcgaccggg acccctacga 2160

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggagaagtcc aagcgcatcc attttttaga cacattaact gggaagagct tttggctcgg 2220

2161 cctcttcagg ttcgcgtagg taaaaaatct gtgtaattga cccttctcga aaaccgagcc 2220

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aaggtggagc gcccctttaa gcctctgttg caatctgaag aggatgtgag tcagtttgat 2280

2221 ttccacctcg cggggaaatt cggagacaac gttagacttc tcctacactc agtcaaacta 2280

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcaaagttta ctcgtcagac acctgttgac agccccgatg actcaactct cagtgaaagt 2340

2281 agtttcaaat gagcagtctg tggacaactg tcggggctac tgagttgaga gtcactttca 2340

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gccaaccagg tctttctggg ttttacatat gtggctccat ctgtacttga aagtgtgaaa 2400

2341 cggttggtcc agaaagaccc aaaatgtata caccgaggta gacatgaact ttcacacttt 2400

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaaaagtttt cttttgaacc aaaaatccga tcgcctcgaa gatttattgg tagcccacga 2460

2401 cttttcaaaa gaaaacttgg tttttaggct agcggagctt ctaaataacc atcgggtgct 2460

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 acgcctgtca gcccagtcaa attctctcct ggggatttct ggggacgagg tgcttcagcc 2520

2461 tgcggacagt cgggtcagtt taagagagga cccctaaaga cccctgctcc acgaagtcgg 2520

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agcacagcaa atcctcagac acctgtggaa tacccaatgg aaacaagtgg aatagagcag 2580

2521 tcgtgtcgtt taggagtctg tggacacctt atgggttacc tttgttcacc ttatctcgtc 2580

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atggatgtga caacgagcgg ggaagcttca gcgccacttc caatccgaca gcccaactct 2640

2581 tacctacact gttgctcgcc ccttcgaagt cgcggtgaag gttaggctgt cgggttgaga 2640

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gggccataca aaaaacaagc ttttcctatg atctccaaac ggccagagca cctgcgtatg 2700

2641 cccggtatgt tttttgttcg aaaaggatac tagaggtttg ccggtctcgt ggacgcatac 2700

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aatctatgat gaaacaatgc ttttattaat gcaaatgcaa aaaggaaaca aatcctggaa 2760

2701 ttagatacta ctttgttacg aaaataatta cgtttacgtt tttcctttgt ttaggacctt 2760

Orf frame 1 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gggcatgtgt gaggatcctg caagataaaa atgagaaaat ggcagtctct aaagagtcag 2820

2761 cccgtacaca ctcctaggac gttctatttt tactctttta ccgtcagaga tttctcagtc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgtcattacc tggaatgctt tcgatggagg aaaaaataaa catggatttt taaaaaaatc 2880

2821 acagtaatgg accttacgaa agctacctcc ttttttattt gtacctaaaa atttttttag 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gaattcgggg gggggggggg gggggacagc tcagggctgc gatttcgcgc caaacttgac 2940

2881 cttaagcccc cccccccccc ccccctgtcg agtcccgacg ctaaagcgcg gtttgaactg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggcaatccta gcgtgaaggc tggtaggatt ttatccccgc tgccatcatg gttcgaccat 3000

2941 ccgttaggat cgcacttccg accatcctaa aataggggcg acggtagtac caagctggta 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgaactgcat cgtcgccgtg tcccaaaata tggggattgg caagaacgga gacctaccct 3060

3001 acttgacgta gcagcggcac agggttttat acccctaacc gttcttgcct ctggatggga 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggcctccgct caggaacgag ttcaagtact tccaaagaat gaccacaacc tcttcagtgg 3120

3061 ccggaggcga gtccttgctc aagttcatga aggtttctta ctggtgttgg agaagtcacc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aaggtaaaca gaatctggtg attatgggta ggaaaacctg gttctccatt cctgagaaga 3180

3121 ttccatttgt cttagaccac taatacccat ccttttggac caagaggtaa ggactcttct 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atcgaccttt aaaggacaga attaatatag ttctcagtag agaactcaaa gaaccaccac 3240

3181 tagctggaaa tttcctgtct taattatatc aagagtcatc tcttgagttt cttggtggtg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... ......... AflII(3278) ......... ..........

3241 gaggagctca ttttcttgcc aaaagtttgg atgatgcctt aagacttatt gaacaaccgg 3300

3241 ctcctcgagt aaaagaacgg ttttcaaacc tactacggaa ttctgaataa cttgttggcc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aattggcaag taaagtagac atggtttgga tagtcggagg cagttctgtt taccaggaag 3360

3301 ttaaccgttc atttcatctg taccaaacct atcagcctcc gtcaagacaa atggtccttc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ccatgaatca accaggccac ctcagactct ttgtgacaag gatcatgcag tgaatttgaa 3420

3361 ggtacttagt tggtccggtg gagtctgaga aacactgttc ctagtacgtc acttaaactt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agtgacacgt ttttcccaga aattgatttg gggaaatata aacttctccc agaataccca 3480

3421 tcactgtgca aaaagggtct ttaactaaac ccctttatat ttgaagaggg tcttatgggt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggcgtcctct ctgaggtcca ggaggaaaaa ggcatcaagt ataagtttga agtctacgag 3540

3481 ccgcaggaga gactccaggt cctccttttt ccgtagttca tattcaaact tcagatgctc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aagaaagact aacaggaaga tgctttcaag ttctctgctc ccctcctaaa gctatgcatt 3600

3541 ttctttctga ttgtccttct acgaaagttc aagagacgag gggaggattt cgatacgtaa 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttttataaga ccatgggact tttgctggct ttagatcata atcagccata ccacatttgt 3660

3601 aaaatattct ggtaccctga aaacgaccga aatctagtat tagtcggtat ggtgtaaaca 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 3720

3661 tctccaaaat gaacgaaatt ttttggaggg tgtggagggg gacttggact ttgtatttta 3720

.......... .......... .......... .......... .......... ..........

.......... HpaI(3739) .......... .......... .......... ..........

3721 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 3780

3721 cttacgttaa caacaacaat tgaacaaata acgtcgaata ttaccaatgt ttatttcgtt 3780

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

3781 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt g tggtttgtc 3840

3781 atcgtagtgt ttaaagtgtt tatttcgtaa aaaaagtgac gtaagatcaa caccaaacag 3840

.......... ......... SV40_PA_term ....... EBV_rev_primer ........

.......... .......... .......... .......... .......... ..........

3841 caaactcatc aatgtatctt atcatgtctg gatccccggc caacggtctg gtgacccggc 3900

3841 gtttgagtag ttacatagaa tagtacagac ctaggggccg gttgccagac cactgggccg 3900

EBV_rev_primer SV40_PA_term .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgcgagagct cggtgtacct gagacgcgag taagcccttg agtcaaagac gtagtcgttg 3960

3901 acgctctcga gccacatgga ctctgcgctc attcgggaac tcagtttctg catcagcaac 3960

.......... .......... .......... .......... .......... ..........

.......... ......... EcoRV(3983) ......... .......... ..........

3961 ctaagtccgc accaggtact gatatcccac caaaaagtgc ggcggcggct ggcggtagag 4020

3961 gattcaggcg tggtccatga ctatagggtg gtttttcacg ccgccgccga ccgccatctc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... BclI(4076)

4021 gggccagcgt agggtggccg gggctccggg ggcgaggtct tccaacataa ggcgatgatc 4080

4021 cccggtcgca tcccaccggc cccgaggccc ccgctccaga aggttgtatt ccgctactag 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atcgatgcta gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg 4140

4081 tagctacgat ctggcacgtt ttcctctcgg acattcgccc gtgagaaggc accagaccac 4140

.......... .......... .......... .......... .......... ..........

.......... .......... ......... BstBI(4179) ......... ..........

4141 gataaattcg caagggtatc atggcggacg accggggttc gaaccccgga tccggccgtc 4200

4141 ctatttaagc gttcccatag taccgcctgc tggccccaag cttggggcct aggccggcag 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgccgtgatc catccggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac 4260

4201 gcggcactag gtaggccaat ggcgggcgca cagcttgggt ccacacgctg cagtctgttg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NheI(4307) ..........

4261 gggggagcgc tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg 4320

4261 ccccctcgcg aggaaaaccg aaggaaggtc cgcgccgccg acgacgcgat cgaaaaaacc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cgagctcgaa ttaattctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 4380

4321 gctcgagctt aattaagacg taattactta gccggttgcg cgcccctctc cgccaaacgc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 4440

4381 ataacccgcg agaaggcgaa ggagcgagtg actgagcgac gcgagccagc aagccgacgc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcgagcggta tcgactcact caaaggcggt aatacggtta tccacagaat caggggataa 4500

4441 cgctcgccat agctgagtga gtttccgcca ttatgccaat aggtgtctta gtcccctatt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 4560

4501 gcgtcctttc ttgtacactc gttttccggt cgttttccgg tccttggcat ttttccggcg 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 4620

4561 caaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga 4620

.......... .......... ......... pBR3_origin ......... ..........

.......... .......... .......... .......... .......... ..........

4621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 4680

4621 gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt 4680

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 4740

4681 cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag 4740

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt 4800

4741 agggaagccc ttcgcaccgc gaaagagtta cgagtgcgac atccatagag tcaagccaca 4800

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 4860

4801 tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc 4860

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 4920

4861 ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc 4920

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 4980

4921 gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga 4980

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 5040

4981 acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag acgcgagacg 5040

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 5100

5041 acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc 5100

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctggtagcgg tggttttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 5160

5101 gaccatcgcc accaaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga 5160

.......... ......... pBR3_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 5220

5161 gttcttctag gaaactagaa aagatgcccc agactgcgag tcaccttgct tttgagtgca 5220

pBR3_origin ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 5280

5221 attccctaaa accagtactc taatagtttt tcctagaagt ggatctagga aaatttaatt 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5340

5281 tttacttcaa aatttagtta gatttcatat atactcattt gaaccagact gtcaatggtt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5400

5341 acgaattagt cactccgtgg atagagtcgc tagacagata aagcaagtag gtatcaacgg 5400

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5460

5401 actgaggggc agcacatcta ttgatgctat gccctcccga atggtagacc ggggtcacga 5460

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5520

5461 cgttactatg gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt 5520

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5580

5521 cggccttccc ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta ggtcagataa 5580

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5640

5581 ttaacaacgg cccttcgatc tcattcatca agcggtcaat tatcaaacgc gttgcaacaa 5640

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5700

5641 cggtaacgat gtccgtagca ccacagtgcg agcagcaaac cataccgaag taagtcgagg 5700

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ggttcccaac gatctaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 5760

5701 ccaagggttg ctagattccg ctcaatgtac tagggggtac aacacgtttt ttcgccaatc 5760

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 5820

5761 gaggaagcca ggaggctagc aacagtcttc attcaaccgg cgtcacaata gtgagtacca 5820

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 5880

5821 ataccgtcgt gacgtattaa gagaatgaca gtacggtagg cattctacga aaagacactg 5880

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 5940

5881 accactcatg agttggttca gtaagactct tatcacatac gccgctggct caacgagaac 5940

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 6000

5941 gggccgcagt tatgccctat tatggcgcgg tgtatcgtct tgaaattttc acgagtagta 6000

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 6060

6001 accttttgca agaagccccg cttttgagag ttcctagaat ggcgacaact ctaggtcaag 6060

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 6120

6061 ctacattggg tgagcacgtg ggttgactag aagtcgtaga aaatgaaagt ggtcgcaaag 6120

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 6180

6121 acccactcgt ttttgtcctt ccgttttacg gcgttttttc ccttattccc gctgtgcctt 6180

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 6240

6181 tacaacttat gagtatgaga aggaaaaagt tataataact tcgtaaatag tcccaataac 6240

Ampicillin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tctcatgagc ggatacatat ttgaattgta tttagaaaaa taaacaaata ggggttccgc 6300

6241 agagtactcg cctatgtata aacttaacat aaatcttttt atttgtttat ccccaaggcg 6300

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 6360

6301 cgtgtaaagg ggcttttcac ggtggactgc agattctttg gtaataatag tactgtaatt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 6420

6361 ggatattttt atccgcatag tgctccggga aagcagagcg cgcaaagcca ctactgccac 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 6480

6421 ttttggagac tgtgtacgtc gagggcctct gccagtgtcg aacagacatt cgcctacggc 6480

........ pGEX_3_primer ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ggagcagaca accccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 6540

6481 cctcgtctgt tggggcagtc ccgcgcagtc gcccacaacc gcccacagcc ccgaccgaat 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 6600

6541 tgatacgccg tagtctcgtc taacatgact ctcacgtggt atacgccaca ctttatggcg 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NarI(6632) .......... ..........

6601 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 6660

6601 tgtctacgca ttcctctttt atggcgtagt ccgcggtaag cggtaagtcc gacgcgttga 6660

.......... .......... .......... .......... .......... lacZ

.......... .......... .......... .......... .......... ..........

6661 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 6720

6661 caacccttcc cgctagccac gcccggagaa gcgataatgc ggtcgaccgc tttcccccta 6720

.......... .......... lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 6780

6721 cacgacgttc cgctaattca acccattgcg gtcccaaaag ggtcagtgct gcaacatttt 6780

.......... .......... lacZ .......... M13_pUC_f ..........

.......... .......... .......... .......... .......... ..........

6781 cgacggccag tgccaagctt .......... .......... .......... .......... 6800

6781 gctgccggtc acggttcgaa .......... .......... .......... .......... 6800

lacZ M13_f20 M13_pUC_f ........ .......... .......... ..........