Sequence of p8100

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of p8100

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of p8100: 8100 BP; 1953 A; 1774 C; 1770 G; 2603 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 60

1 ttacgatgat gataatcatc ttaactacgg tggaaaagtc gagcgcgggg tttactttta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 120

61 tatcgatttg tccaataact ggtaaacgct ttacatagat taccagtttg atttagatga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgttcgcaga attgggaatc aactgttata tggaatgaaa cttccagaca ccgtacttta 180

121 gcaagcgtct taacccttag ttgacaatat accttacttt gaaggtctgt ggcatgaaat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gttgcatatt taaaacatgt tgagctacag cattatattc agcaattaag ctctaagcca 240

181 caacgtataa attttgtaca actcgatgtc gtaatataag tcgttaattc gagattcggt 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 300

241 aggcgttttt actggagaat agttttcctc gttaatttcc atgagagatt aggactggac 300

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 360

301 aacctcaaac gaaggccaga ccaagcgaaa cttcgagctt aattttgcgc tataaacttc 360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt 420

361 agaaagcccg aaggagaatt agaaaaacta cgttaggcga aacgaagact gatattatca 420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 480

421 gtcccatttc tggactaaaa actaaatacc agtaagagca aaagacttga caaatttcgt 480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 540

481 aaactccccc taagttactt ataaatactg ctaaggcgtc ataacctgcg ataggtcaga 540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 600

541 tttgtaaaat gataatgggg gagaccgttt tgaagaaaac gttttcggag agcgataaaa 600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 660

601 ccaaaaatag cagcagacca tttgctccca atactatcac aacgagaatg atacggagca 660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 720

661 ttaaggaaaa ccgcaataca tagacgtaat caacttacac cataaggatt tagagttgac 720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 780

721 tacttagaaa gatggacatt attacaacaa ggcaatcaag caaaataatt gcatctaaaa 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 840

781 agaagggttg caggactgac catattactc ggtcaagaat tttagcgtat tccattaagt 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt 900

841 gttactaatt tcaactttaa tttggtagag ttcgggttaa atgatgagca agaccacaaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 960

901 gagcagtccc gttcggaata agtgacttac tcgtcgaaac aatgcaacta aacccattac 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc 1020

961 ttataggcca agaacagttc taatgagaac tacttccagt cggtcggata cgcggaccag 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc 1080

1021 acatgtggca agtagacagg agaaagtttc aaccagtcaa gccaagggaa tactaactgg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1140

1081 cagacgcgga gcaaggccga ttcattgtac ctcgtccagc gcctaaagct gtgttaaata 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1200

1141 gtccgctact atgtttagag gcaacatgaa acaaagcgcg aaccatatta gcgaccccca 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 caaagatgag tgttttagtg tattcttttg cctctttcgt tttaggttgg tgccttcgta 1260

1201 gtttctactc acaaaatcac ataagaaaac ggagaaagca aaatccaacc acggaagcat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct 1320

1261 caccgtaatg cataaaatgg gcaaattacc tttgaaggag tactttttca gaaatcagga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1380

1321 gtttcggaga catcggcaac gatgggagca aggctacgac agaaagcgac gactcccact 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta 1440

1381 gctagggcgt tttcgccgga aattgaggga cgttcggagt cgctggctta tatagccaat 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1500

1441 acgcacccgc taccaacaac agtaacagcc gcgttgatag ccatagttcg acaaattctt 1500

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 1560

1501 taagtggagc tttcgttcga ctatttggct atgttaattt ccgaggaaaa cctcggaaaa 1560

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttttggagat tttcaacgtg aaaaaattat tattcgcaat tcctttagtt gttcctttct 1620

1561 aaaacctcta aaagttgcac ttttttaata ataagcgtta aggaaatcaa caaggaaaga 1620

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 attctcactc cgctgaaact gttgaaagtt gtttagcaaa atcccataca gaaaattcat 1680

1621 taagagtgag gcgactttga caactttcaa caaatcgttt tagggtatgt cttttaagta 1680

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttactaacgt ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggctgtc 1740

1681 aatgattgca gacctttctg ctgttttgaa atctagcaat gcgattgata ctcccgacag 1740

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgtggaatgc tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat 1800

1741 acaccttacg atgtccgcaa catcaaacat gaccactgct ttgagtcaca atgccatgta 1800

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggttcctat tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt 1860

1801 cccaaggata acccgaacga tagggacttt tactcccacc accgagactc ccaccgccaa 1860

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctgagggtgg cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta 1920

1861 gactcccacc gccaagactc ccaccgccat gatttggagg actcatgcca ctatgtggat 1920

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttccgggcta tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa 1980

1921 aaggcccgat atgaatatag ttgggagagc tgccgtgaat aggcggacca tgactcgttt 1980

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 accccgctaa tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc 2040

1981 tggggcgatt aggattagga agagaactcc tcagagtcgg agaattatga aagtacaaag 2040

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agaataatag gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc 2100

2041 tcttattatc caaggcttta tccgtccccc gtaattgaca aatatgcccg tgacaatgag 2100

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaggcactga ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt 2160

2101 ttccgtgact ggggcaattt tgaataatgg tcatgtgagg acatagtagt tttcggtaca 2160

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 atgacgctta ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgagg 2220

2161 tactgcgaat gaccttgcca tttaagtctc tgacgcgaaa ggtaagaccg aaattactcc 2220

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atttatttgt ttgtgaatat caaggccaat cgtctgacct gcctcaacct cctgtcaatg 2280

2221 taaataaaca aacacttata gttccggtta gcagactgga cggagttgga ggacagttac 2280

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc tctgagggtg 2340

2281 gaccgccgcc gagaccacca ccaagaccac cgccgagact cccaccaccg agactcccac 2340

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg 2400

2341 cgccaagact cccaccgccg agactccctc cgccaaggcc accaccgaga ccaaggccac 2400

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg 2460

2401 taaaactaat acttttctac cgtttgcgat tattcccccg atactggctt ttacggctac 2460

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg 2520

2461 ttttgcgcga tgtcagactg cgatttccgt ttgaactaag acagcgatga ctaatgccac 2520

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg 2580

2521 gacgatagct accaaagtaa ccactgcaaa ggccggaacg attaccatta ccacgatgac 2580

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt 2640

2581 cactaaaacg accgagatta agggtttacc gagttcagcc actgccacta ttaagtggaa 2640

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt 2700

2641 attacttatt aaaggcagtt ataaatggaa gggagggagt tagccaactt acagcgggaa 2700

.......... .......... ORF 4 .......... .......... ..........

.......... .......... NdeI(2724) .......... .......... ..........

2701 ttgtctttgg cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat 2760

2701 aacagaaacc gcgaccattt ggtatactta aaagataact aacactgttt tatttgaata 2760

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt 2820

2761 aggcaccaca gaaacgcaaa gaaaatatac aacggtggaa atacatacat aaaagatgca 2820

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttgctaacat actgcgtaat aaggagtctt aatcatgcca gttcttttgg gtattccgtt 2880

2821 aacgattgta tgacgcatta ttcctcagaa ttagtacggt caagaaaacc cataaggcaa 2880

.......... ORF 4 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 attattgcgt ttcctcggtt tccttctggt aactttgttc ggctatctgc ttacttttct 2940

2881 taataacgca aaggagccaa aggaagacca ttgaaacaag ccgatagacg aatgaaaaga 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taaaaagggc ttcggtaaga tagctattgc tatttcattg tttcttgctc ttattattgg 3000

2941 atttttcccg aagccattct atcgataacg ataaagtaac aaagaacgag aataataacc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcttaactca attcttgtgg gttatctctc tgatattagc gctcaattac cctctgactt 3060

3001 cgaattgagt taagaacacc caatagagag actataatcg cgagttaatg ggagactgaa 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgttcagggt gttcagttaa ttctcccgtc taatgcgctt ccctgttttt atgttattct 3120

3061 acaagtccca caagtcaatt aagagggcag attacgcgaa gggacaaaaa tacaataaga 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctctgtaaag gctgctattt tcatttttga cgttaaacaa aaaatcgttt cttatttgga 3180

3121 gagacatttc cgacgataaa agtaaaaact gcaatttgtt ttttagcaaa gaataaacct 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttgggataaa taatatggct gtttattttg taactggcaa attaggctct ggaaagacgc 3240

3181 aaccctattt attataccga caaataaaac attgaccgtt taatccgaga cctttctgcg 3240

.......... .......... .......... ORF 5 .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcgttagcgt tggtaagatt caggataaaa ttgtagctgg gtgcaaaata gcaactaatc 3300

3241 agcaatcgca accattctaa gtcctatttt aacatcgacc cacgttttat cgttgattag 3300

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttgatttaag gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg cctcgcgttc 3360

3301 aactaaattc cgaagttttg gagggcgttc agccctccaa gcgattttgc ggagcgcaag 3360

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttagaatacc ggataagcct tctatatctg atttgcttgc tattgggcgc ggtaatgatt 3420

3361 aatcttatgg cctattcgga agatatagac taaacgaacg ataacccgcg ccattactaa 3420

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctacgatga aaataaaaac ggcttgcttg ttctcgatga gtgcggtact tggtttaata 3480

3421 ggatgctact tttatttttg ccgaacgaac aagagctact cacgccatga accaaattat 3480

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cccgttcttg gaatgataag gaaagacagc cgattattga ttggtttcta catgctcgta 3540

3481 gggcaagaac cttactattc ctttctgtcg gctaataact aaccaaagat gtacgagcat 3540

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aattaggatg ggatattatt tttcttgttc aggacttatc tattgttgat aaacaggcgc 3600

3541 ttaatcctac cctataataa aaagaacaag tcctgaatag ataacaacta tttgtccgcg 3600

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gttctgcatt agctgaacat gttgtttatt gtcgtcgtct ggacagaatt actttacctt 3660

3601 caagacgtaa tcgacttgta caacaaataa cagcagcaga cctgtcttaa tgaaatggaa 3660

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttgtcggtac tttatattct cttattactg gctcgaaaat gcctctgcct aaattacatg 3720

3661 aacagccatg aaatataaga gaataatgac cgagctttta cggagacgga tttaatgtac 3720

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttggcgttgt taaatatggc gattctcaat taagccctac tgttgagcgt tggctttata 3780

3721 aaccgcaaca atttataccg ctaagagtta attcgggatg acaactcgca accgaaatat 3780

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctggtaagaa tttgtataac gcatatgata ctaaacaggc tttttctagt aattatgatt 3840

3781 gaccattctt aaacatattg cgtatactat gatttgtccg aaaaagatca ttaatactaa 3840

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ccggtgttta ttcttattta acgccttatt tatcacacgg tcggtatttc aaaccattaa 3900

3841 ggccacaaat aagaataaat tgcggaataa atagtgtgcc agccataaag tttggtaatt 3900

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atttaggtca gaagatgaaa ttaactaaaa tatatttgaa aaagttttct cgcgttcttt 3960

3901 taaatccagt cttctacttt aattgatttt atataaactt tttcaaaaga gcgcaagaaa 3960

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtcttgcgat tggatttgca tcagcattta catatagtta tataacccaa cctaagccgg 4020

3961 cagaacgcta acctaaacgt agtcgtaaat gtatatcaat atattgggtt ggattcggcc 4020

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aggttaaaaa ggtagtctct cagacctatg attttgataa attcactatt gactcttctc 4080

4021 tccaattttt ccatcagaga gtctggatac taaaactatt taagtgataa ctgagaagag 4080

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... PacI(4136)

4081 agcgtcttaa tctaagctat cgctatgttt tcaaggattc taagggaaaa ttaattaata 4140

4081 tcgcagaatt agattcgata gcgatacaaa agttcctaag attccctttt aattaattat 4140

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcgacgattt acagaagcaa ggttattcac tcacatatat tgatttatgt actgtttcca 4200

4141 cgctgctaaa tgtcttcgtt ccaataagtg agtgtatata actaaataca tgacaaaggt 4200

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttaaaaaagg taattcaaat gaaattgtta aatgtaatta attttgtttt cttgatgttt 4260

4201 aattttttcc attaagttta ctttaacaat ttacattaat taaaacaaaa gaactacaaa 4260

.......... .......... ORF 5 ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtttcatcat cttcttttgc tcaggtaatt gaaatgaata attcgcctct gcgcgatttt 4320

4261 caaagtagta gaagaaaacg agtccattaa ctttacttat taagcggaga cgcgctaaaa 4320

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gtaacttggt attcaaagca atcaggcgaa tccgttattg tttctcccga tgtaaaaggt 4380

4321 cattgaacca taagtttcgt tagtccgctt aggcaataac aaagagggct acattttcca 4380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 actgttactg tatattcatc tgacgttaaa cctgaaaatc tacgcaattt ctttatttct 4440

4381 tgacaatgac atataagtag actgcaattt ggacttttag atgcgttaaa gaaataaaga 4440

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gttttacgtg caaataattt tgatatggta ggttctaacc cttccattat tcagaagtat 4500

4441 caaaatgcac gtttattaaa actataccat ccaagattgg gaaggtaata agtcttcata 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aatccaaaca atcaggatta tattgatgaa ttgccatcat ctgataatca ggaatatgat 4560

4501 ttaggtttgt tagtcctaat ataactactt aacggtagta gactattagt ccttatacta 4560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gataattccg ctccttctgg tggtttcttt gttccgcaaa atgataatgt tactcaaact 4620

4561 ctattaaggc gaggaagacc accaaagaaa caaggcgttt tactattaca atgagtttga 4620

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tttaaaatta ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt gtttgtaaag 4680

4621 aaattttaat tattgcaagc ccgtttccta aattatgctc aacagcttaa caaacatttc 4680

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tctaatactt ctaaatcctc aaatgtatta tctattgacg gctctaatct attagttgtt 4740

4681 agattatgaa gatttaggag tttacataat agataactgc cgagattaga taatcaacaa 4740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agtgctccta aagatatttt agataacctt cctcaattcc tttcaactgt tgatttgcca 4800

4741 tcacgaggat ttctataaaa tctattggaa ggagttaagg aaagttgaca actaaacggt 4800

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 actgaccaga tattgattga gggtttgata tttgaggttc agcaaggtga tgctttagat 4860

4801 tgactggtct ataactaact cccaaactat aaactccaag tcgttccact acgaaatcta 4860

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttttcatttg ctgctggctc tcagcgtggc actgttgcag gcggtgttaa tactgaccgc 4920

4861 aaaagtaaac gacgaccgag agtcgcaccg tgacaacgtc cgccacaatt atgactggcg 4920

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ctcacctctg ttttatcttc tgctggtggt tcgttcggta tttttaatgg cgatgtttta 4980

4921 gagtggagac aaaatagaag acgaccacca agcaagccat aaaaattacc gctacaaaat 4980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gggctatcag ttcgcgcatt aaagactaat agccattcaa aaatattgtc tgtgccacgt 5040

4981 cccgatagtc aagcgcgtaa tttctgatta tcggtaagtt tttataacag acacggtgca 5040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 attcttacgc tttcaggtca gaagggttct atctctgttg gccagaatgt cccttttatt 5100

5041 taagaatgcg aaagtccagt cttcccaaga tagagacaac cggtcttaca gggaaaataa 5100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 actggtcgtg tgactggtga atctgccaat gtaaataatc catttcagac gattgagcgt 5160

5101 tgaccagcac actgaccact tagacggtta catttattag gtaaagtctg ctaactcgca 5160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt 5220

5161 gttttacatc cataaaggta ctcgcaaaaa ggacaacgtt accgaccgcc attataacaa 5220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt 5280

5221 gacctataat ggtcgttccg gctatcaaac tcaagaagat gagtccgttc actacaataa 5280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 actaatcaaa gaagtattgc tacaacggtt aatttgcgtg atggacagac tcttttactc 5340

5281 tgattagttt cttcataacg atgttgccaa ttaaacgcac tacctgtctg agaaaatgag 5340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt cctgtctaaa 5400

5341 ccaccggagt gactaatatt tttgtgaaga gtcctaagac cgcatggcaa ggacagattt 5400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga aagcacgtta 5460

5401 tagggaaatt agccggagga caaatcgagg gcgagactaa gattgctcct ttcgtgcaat 5460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 5520

5461 atgcacgagc agtttcgttg gtatcatgcg cgggacatcg ccgcgtaatt cgcgccgccc 5520

.......... ORF 2 .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

5521 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 5580

5521 acaccaccaa tgcgcgtcgc actggcgatg tgaacggtcg cgggatcgcg ggcgaggaaa 5580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 5640

5581 gcgaaagaag ggaaggaaag agcggtgcaa gcggccgaaa ggggcagttc gagatttagc 5640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 5700

5641 ccccgaggga aatcccaagg ctaaatcacg aaatgccgtg gagctggggt tttttgaact 5700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 5760

5701 aaacccacta ccaagtgcat cacccggtag cgggactatc tgccaaaaag cgggaaactg 5760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 5820

5761 caacctcagg tgcaagaaat tatcacctga gaacaaggtt tgaccttgtt gtgagttggg 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa 5880

5821 atagagcccg ataagaaaac taaatattcc ctaaaacggc taaagccttg gtggtagttt 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 5940

5881 gtcctaaaag cggacgaccc cgtttggtcg cacctggcga acgacgttga gagagtcccg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 6000

5941 gtccgccact tcccgttagt cgacaacggg cagagtgacc acttttcttt ttggtgggac 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 6060

6001 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 6120

6061 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattac actcaatcga 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 6180

6121 gtgagtaatc cgtggggtcc gaaatgtgaa atacgaaggc cgagcataca acacacctta 6180

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

6181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct 6240

6181 acactcgcct attgttaaag tgtgtccttt gtcgatactg gtactaatgc ttaagctcga 6240

...... M13_pUC_rev_primer .. M13_reverse_primer ...... ..........

SacI XmaI(6247),KpnI(6247),SmaI(6249),BamHI(6252) ......

6241 cggtacccgg ggatcctcaa ctgtgaggag gctcacggac gcgaagaaca ggcacgcgtg 6300

6241 gccatgggcc cctaggagtt gacactcctc cgagtgcctg cgcttcttgt ccgtgcgcac 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ctggcagaaa cccccggtat gaccgtgaaa acggcccgcc gcattctggc cgcagcacca 6360

6301 gaccgtcttt gggggccata ctggcacttt tgccgggcgg cgtaagaccg gcgtcgtggt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cagagtgcac aggcgcgcag tgacactgcg ctggatcgtc tgatgcaggg ggcaccggca 6420

6361 gtctcacgtg tccgcgcgtc actgtgacgc gacctagcag actacgtccc ccgtggccgt 6420

.......... .......... .......... .......... .......... ..........

.......... PstI(6433) .......... HpaI(6457) .......... ..........

6421 ccgctggctg caggtaaccc ggcatctgat gccgttaacg atttgctgaa cacaccagtg 6480

6421 ggcgaccgac gtccattggg ccgtagacta cggcaattgc taaacgactt gtgtggtcac 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 taagggatgt ttatgacgag caaagaaacc tttacccatt accagccgca gggcaacagt 6540

6481 attccctaca aatactgctc gtttctttgg aaatgggtaa tggtcggcgt cccgttgtca 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gacccggctc ataccgcaac cgcgcccggc ggattgagtg cgaaagcgcc tgcaatgacc 6600

6541 ctgggccgag tatggcgttg gcgcgggccg cctaactcac gctttcgcgg acgttactgg 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ccgctgatgc tggacacctc cagccgtaag ctggttgcgt gggatggcac caccgacggt 6660

6601 ggcgactacg acctgtggag gtcggcattc gaccaacgca ccctaccgtg gtggctgcca 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gctgccgttg gcattcttgc ggttgctgct gaccagacca gcaccacgct gacgttctac 6720

6661 cgacggcaac cgtaagaacg ccaacgacga ctggtctggt cgtggtgcga ctgcaagatg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 aagtccggca cgttccgtta tgaggatgtg ctctggccgg aggctgccag cgacgagacg 6780

6721 ttcaggccgt gcaaggcaat actcctacac gagaccggcc tccgacggtc gctgctctgc 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aaaaaacgga ccgcgtttgc cggaacggca atcagcatcg tttaacttta cccttcatca 6840

6781 ttttttgcct ggcgcaaacg gccttgccgt tagtcgtagc aaattgaaat gggaagtagt 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ctaaaggccg cctgtgcggc tttttttacg ggattttttt atgtcgatgt acacaaccgc 6900

6841 gatttccggc ggacacgccg aaaaaaatgc cctaaaaaaa tacagctaca tgtgttggcg 6900

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

6901 ccaactgctg gcggcaaatg agcagaaatt taagtttgat ccgctgtttc tgcgtctctt 6960

6901 ggttgacgac cgccgtttac tcgtctttaa attcaaacta ggcgacaaag acgcagagaa 6960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tttccgtgag agctatccct tcaccacgga gaaagtctat ctctcacaaa ttccgggact 7020

6961 aaaggcactc tcgataggga agtggtgcct ctttcagata gagagtgttt aaggccctga 7020

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ggtaaacatg gcgctgtacg tttcgccgat tgtttccggt gaggttatcc gttcccgtgg 7080

7021 ccatttgtac cgcgacatgc aaagcggcta acaaaggcca ctccaatagg caagggcacc 7080

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cggctccacc tctgaattta cgccgggata tgtcaagccg aagcatgaag taagcttggc 7140

7081 gccgaggtgg agacttaaat gcggccctat acagttcggc ttcgtacttc attcgaaccg 7140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 7200

7141 tgaccggcag caaaatgttg cagcactgac ccttttggga ccgcaatggg ttgaattagc 7200

M13_forward20_primer ORF 3 M13_pUC_fwd_primer ...... ..........

.......... .......... .......... .......... .......... ..........

7201 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 7260

7201 ggaacgtcgt gtagggggaa agcggtcgac cgcattatcg cttctccggg cgtggctagc 7260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc 7320

7261 gggaagggtt gtcaacgcgt cggacttacc gcttaccgcg aaacggacca aaggccgtgg 7320

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt 7380

7321 tcttcgccac ggcctttcga ccgacctcac gctagaagga ctccggctat gacagcagca 7380

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc 7440

7381 ggggagtttg accgtctacg tgccaatgct acgcgggtag atgtggttgc actggatagg 7440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac 7500

7441 gtaatgccag ttaggcggca aacaagggtg cctcttaggc tgcccaacaa tgagcgagtg 7500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt 7560

7501 taaattacaa ctactttcga ccgatgtcct tccggtctgc gcttaataaa aactaccgca 7560

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 tcctattggt taaaaaatga gctgatttaa caaaaattta atgcgaattt taacaaaata 7620

7561 aggataacca attttttact cgactaaatt gtttttaaat tacgcttaaa attgttttat 7620

ORF 3 .......... .......... .......... .......... ..........

.......... SwaI(7637) .......... .......... .......... ..........

7621 ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga 7680

7621 aattgcaaat gttaaattta taaacgaata tgttagaagg acaaaaaccc cgaaaagact 7680

.......... .......... .......... .......... .......... ..........

.......... NdeI(7698) .......... .......... .......... ..........

7681 ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt catcgattct 7740

7681 aatagttggc cccatgtata ctaactgtac gatcaaaatg ctaatggcaa gtagctaaga 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... BglII(7786) .........

7741 cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagatct ctcaaaaata 7800

7741 gaacaaacga ggtctgagag tccgttactg gactatcgga aacatctaga gagtttttat 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 gctaccctct ccggcattaa tttatcagct agaacggttg aatatcatat tgatggtgat 7860

7801 cgatgggaga ggccgtaatt aaatagtcga tcttgccaac ttatagtata actaccacta 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ttgactgtct ccggcctttc tcaccctttt gaatctttac ctacacatta ctcaggcatt 7920

7861 aactgacaga ggccggaaag agtgggaaaa cttagaaatg gatgtgtaat gagtccgtaa 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 gcatttaaaa tatatgaggg ttctaaaaat ttttatcctt gcgttgaaat aaaggcttct 7980

7921 cgtaaatttt atatactccc aagattttta aaaataggaa cgcaacttta tttccgaaga 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 cccgcaaaag tattacaggg tcataatgtt tttggtacaa ccgatttagc tttatgctct 8040

7981 gggcgttttc ataatgtccc agtattacaa aaaccatgtt ggctaaatcg aaatacgaga 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gaggctttat tgcttaattt tgctaattct ttgccttgcc tgtatgattt attggatgtt 8100

8041 ctccgaaata acgaattaaa acgattaaga aacggaacgg acatactaaa taacctacaa 8100

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-8100 Reference author: Douglas S.M., Dietz H., Liedl T., Hogberg B., Graf F., Shih W.M.; Reference title: "Self-assembly of DNA into nanoscale three-dimensional shapes"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-8100 Reference author: Su X.; Reference title: ; Reference location: Submitted (16-DEC-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Cancer Biology, Dana Farber Cancer Institute, 44 Binney St, Boston, MA Reference location: 02115, USA