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    Plasmid map of pAC-Kan-alphaGal4

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    View the sequence with features on line
    Gene/insert name: RNAP alpha-Gal4
    Insert size (bp): 900
    Species of gene(s): Other
    Vector backbone: pACLU-Kan (Search Vector Database)
    Type of vector: Bacterial expression
    Backbone size (bp): 4506
    5' Sequencing primer: AGA GCC TGA TAA AAA CGG TTA GCG (List of Sequencing Primers)
    Bacteria resistance: Kanamycin
    High or low copy: Low Copy
    Grow in standard E. coli @ 37C: No
    Please specify bacterial strain for growth and growth condition: Needs strain with lacIq
    Sequence: View sequence
    Author's Map: View map
    Plasmid Provided In: XL1 Blue
    Principal Investigator: Keith Joung
    Comments: Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.
    This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.

    image of pAC-Kan-alphaGal4

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