Sequence of pACAGW-ChR2-Venus-AAV

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Sequence of pACAGW-ChR2-Venus-AAV

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Sequence of pACAGW-ChR2-Venus-AAV : 7263 BP; 1511 A; 1908 C; 1868 G; 1694 T; 282 other;

.......... .......... .......... .......... .......... ..........

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggtctaaat 240

....... M13_pUC_rev_pri .... M13_reverse_pri ....... ..........

.......... .......... .......... .......... .......... ..........

241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc 300

241 taattccgga attaatccga cgcgcgagcg agcgagtgac tccggcgggc ccgtttcggg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga 360

301 cccgcagccc gctggaaacc agcgggccgg agtcactcgc tcgctcgcgc gtctctccct 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta 420

361 caccggttga ggtagtgatc cccaaggaac atcaattact aattgggcgg tacgatgaat 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tctacgtagc catgctctag gaagatcgta ccattgacgt caataatgac gtatgttccc 480

421 agatgcatcg gtacgagatc cttctagcat ggtaactgca gttattactg catacaaggg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt acggtaaact 540

481 tatcattgcg gttatccctg aaaggtaact gcagttaccc acctgataaa tgccatttga 540

.......... .......... .......... .......... .......... ..........

.......... .......... NdeI(569) .......... .......... ..........

541 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat 600

541 cgggtgaacc gtcatgtagt tcacatagta tacggttcat gcgggggata actgcagtta 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact 660

601 ctgccattta ccgggcggac cgtaatacgg gtcatgtact ggaataccct gaaaggatga 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc 720

661 accgtcatgt agatgcataa tcagtagcga taatggtacc agctccactc ggggtgcaag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt 780

721 acgaagtgag aggggtagag gggggggagg ggtgggggtt aaaacataaa taaataaaaa 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg 840

781 attaataaaa cacgtcgcta cccccgcccc cccccccccc ccgcgcgcgg tccgccccgc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc 900

841 cccgccccgc tccccgcccc gccccgctcc gcctctccac gccgccgtcg gttagtctcg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag 960

901 ccgcgcgagg ctttcaaagg aaaataccgc tccgccgccg ccgccgccgg gatatttttc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgaagcgcgc ggcgggcggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc 1020

961 gcttcgcgcg ccgcccgccc tcagcgacgc tgcgacggaa gcggggcacg gggcgaggcg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 1080

1021 gcggcggagc gcggcgggcg gggccgagac tgactggcgc aatgagggtg tccactcgcc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 1140

1081 cgccctgccg ggaagaggag gcccgacatt aatcgcgaac caaattactg ccgaacaaag 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ApaI(1184) ..........

1141 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 1200

1141 aaaagacacc gacgcacttt cggaactccc cgaggccctc ccgggaaaca cgcccccctc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cggctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt 1260

1201 gccgagcccc gacaggcgcc cccctgccga cggaagcccc ccctgccccg tcccgcccca 1260

.......... .......... .......... .......... .......... ..........

.......... .......... XbaI(1287) .......... .......... ..........

1261 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct 1320

1261 agccgaagac cgcacactgg ccgccgagat ctcggagacg attggtacaa gtacggaaga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca 1380

1321 agaaaaagga tgtcgaggac ccgttgcacg accaataaca cgacagagta gtaaaaccgt 1380

.......... .......... .......... pCAG_F_pri .......... ..........

......... BamHI(1394) NheI(1406) ......... .......... ..........

1381 aagaattgga tcgggatcca ccggtgctag catggattat ggaggcgccc tgagtgccgt 1440

1381 ttcttaacct agccctaggt ggccacgatc gtacctaata cctccgcggg actcacggca 1440

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

1441 tgggcgcgag ctgctatttg taacgaaccc agtagtcgtc aatggctctg tacttgtgcc 1500

1441 acccgcgctc gacgataaac attgcttggg tcatcagcag ttaccgagac atgaacacgg 1500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgaggaccag tgttactgcg cgggctggat tgagtcgcgt ggcacaaacg gtgcccaaac 1560

1501 actcctggtc acaatgacgc gcccgaccta actcagcgca ccgtgtttgc cacgggtttg 1560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggcgtcgaac gtgctgcaat ggcttgctgc tggcttctcc atcctactgc ttatgtttta 1620

1561 ccgcagcttg cacgacgtta ccgaacgacg accgaagagg taggatgacg aatacaaaat 1620

.......... .......... ORF 2 .......... .......... ..........

.......... .......... ......... BglII(1659) ......... ..........

1621 cgcctaccaa acatggaagt caacctgcgg ctgggaggag atctatgtgt gcgctatcga 1680

1621 gcggatggtt tgtaccttca gttggacgcc gaccctcctc tagatacaca cgcgatagct 1680

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gatggtcaag gtgattctcg agttcttctt cgagtttaag aacccgtcca tgctgtatct 1740

1681 ctaccagttc cactaagagc tcaagaagaa gctcaaattc ttgggcaggt acgacataga 1740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agccacaggc caccgcgtcc agtggttgcg ttacgccgag tggcttctca cctgcccggt 1800

1741 tcggtgtccg gtggcgcagg tcaccaacgc aatgcggctc accgaagagt ggacgggcca 1800

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cattctcatt cacctgtcaa acctgacggg cttgtccaac gactacagca ggcgcaccat 1860

1801 gtaagagtaa gtggacagtt tggactgccc gaacaggttg ctgatgtcgt ccgcgtggta 1860

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gggtctgctt gtgtctgata ttggcacaat tgtgtggggc gccacttccg ccatggccac 1920

1861 cccagacgaa cacagactat aaccgtgtta acacaccccg cggtgaaggc ggtaccggtg 1920

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cggatacgtc aaggtcatct tcttctgcct gggtctgtgt tatggtgcta acacgttctt 1980

1921 gcctatgcag ttccagtaga agaagacgga cccagacaca ataccacgat tgtgcaagaa 1980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcacgctgcc aaggcctaca tcgagggtta ccacaccgtg ccgaagggcc ggtgtcgcca 2040

1981 agtgcgacgg ttccggatgt agctcccaat ggtgtggcac ggcttcccgg ccacagcggt 2040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggtggtgact ggcatggctt ggctcttctt cgtatcatgg ggtatgttcc ccatcctgtt 2100

2041 ccaccactga ccgtaccgaa ccgagaagaa gcatagtacc ccatacaagg ggtaggacaa 2100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 catcctcggc cccgagggct tcggcgtcct gagcgtgtac ggctccaccg tcggccacac 2160

2101 gtaggagccg gggctcccga agccgcagga ctcgcacatg ccgaggtggc agccggtgtg 2160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 catcattgac ctgatgtcga agaactgctg gggtctgctc ggccactacc tgcgcgtgct 2220

2161 gtagtaactg gactacagct tcttgacgac cccagacgag ccggtgatgg acgcgcacga 2220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gatccacgag catatcctca tccacggcga cattcgcaag accaccaaat tgaacattgg 2280

2221 ctaggtgctc gtataggagt aggtgccgct gtaagcgttc tggtggttta acttgtaacc 2280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggcactgag attgaggtcg agacgctggt ggaggacgag gccgaggctg gcgcggtcaa 2340

2281 accgtgactc taactccagc tctgcgacca cctcctgctc cggctccgac cgcgccagtt 2340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caagggcacc ggcaagccac cggtcgccac catggtgagc aagggcgagg a gctgttcac 2400

2341 gttcccgtgg ccgttcggtg gccagcggtg gtaccactcg ttcccgctcc tcgacaagtg 2400

.......... .......... ORF 2 .......... ECFP ..........

.......... .......... .......... .......... .......... ..........

2401 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 2460

2401 gccccaccac gggtaggacc agctcgacct gccgctgcat ttgccggtgt tcaagtcgca 2460

.......... .... ORF 2 ECFP EGFP_N_pri .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac 2520

2461 caggccgctc ccgctcccgc tacggtggat gccgttcgac tgggacttcg actagacgtg 2520

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... PstI(2580)

2521 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca 2580

2521 gtggccgttc gacgggcacg ggaccgggtg ggagcactgg tgggacccga tgccggacgt 2580

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 2640

2581 cacgaagcgg gcgatggggc tggtgtactt cgtcgtgctg aagaagttca ggcggtacgg 2640

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 2700

2641 gcttccgatg caggtcctcg cgtggtagaa gaagttcctg ctgccgttga tgttctgggc 2700

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 2760

2701 gcggctccac ttcaagctcc cgctgtggga ccacttggcg tagctcgact tcccgtagct 2760

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 2820

2761 gaagttcctc ctgccgttgt aggaccccgt gttcgacctc atgttgatgt tgtcggtgtt 2820

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca 2880

2821 gcagatatag tggcggctgt tcgtcttctt gccgtagttc cggttgaagt tctaggcggt 2880

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 2940

2881 gttgtagctc ctgccgccgc acgtcgagcg gctggtgatg gtcgtcttgt gggggtagcc 2940

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa 3000

2941 gctgccgggg cacgacgacg ggctgttggt gatggactcg atggtcaggt tcgactcgtt 3000

.......... .......... ORF 2 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 3060

3001 tctggggttg ctcttcgcgc tagtgtacca ggacgacctc aagcactggc ggcggcccta 3060

.......... .......... ORF 2 ECFP EGFP_C_pri .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cactctcggc atggacgagc tgtacaagta aagcggcccc ggactcgagg ccgcaggtaa 3120

3061 gtgagagccg tacctgctcg acatgttcat ttcgccgggg cctgagctcc ggcgtccatt 3120

.......... ORF 2 ECFP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtatcaaggt tacaagacag gtttaaggag accacataga aactgggctt gtcgagacag 3180

3121 catagttcca atgttctgtc caaattcctc tggtgtatct ttgacccgaa cagctctgtc 3180

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt 3240

3181 tcttctgaga acgcaaagac tatccgtgga taaccagaat gactgtaggt gaaacggaaa 3240

.......... .......... chim_int .......... .......... ..........

SacI,NruI,SpeI BstBI,EcoRI EcoRV90),HindIII(3294)

3241 ctctccacag gtgtcgagtg gagctcgcga ctagtcgatt cgaattcgat atcaagctta 3300

3241 gagaggtgtc cacagctcac ctcgagcgct gatcagctaa gcttaagcta tagttcgaat 3300

chim_int .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 3360

3301 agctattagt tggagaccta atgttttaaa cactttctaa ctgaccataa gaattgatac 3360

.......... .......... .......... WPRE .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 3420

3361 aacgaggaaa atgcgataca cctatgcgac gaaattacgg aaacatagta cgataacgaa 3420

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 3480

3421 gggcataccg aaagtaaaag aggaggaaca tatttaggac caacgacaga gaaatactcc 3480

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 3540

3481 tcaacaccgg gcaacagtcc gttgcaccgc accacacgtg acacaaacga ctgcgttggg 3540

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 3600

3541 ggtgaccaac cccgtaacgg tggtggacag tcgaggaaag gccctgaaag cgaaaggggg 3600

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 3660

3601 agggataacg gtgccgcctt gagtagcggc ggacggaacg ggcgacgacc tgtccccgag 3660

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc 3720

3661 ccgacaaccc gtgactgtta aggcaccaca acagcccctt tagtagcagg aaaggaaccg 3720

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 3780

3721 acgagcggac acaacggtgg acctaagacg cgccctgcag gaagacgatg cagggaagcc 3780

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 3840

3781 gggagttagg tcgcctggaa ggaagggcgc cggacgacgg ccgagacgcc ggagaaggcg 3840

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat 3900

3841 cagaagcgga agcgggagtc tgctcagcct agagggaaac ccggcggagg ggcgtagcta 3900

.......... .......... WPRE .......... .......... ..........

SalI(3904) NotI(3915),EagI(3915) ........ .......... ..........

3901 accgtcgacc cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac 3960

3901 tggcagctgg gcccgccggc gaagctcgtc tgtactattc tatgtaacta ctcaaacctg 3960

.......... .......... .......... ... SV40_PA_term EBV_rev_pri ...

.......... .......... .......... .......... .......... ..........

3961 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg 4020

3961 tttggtgttg atcttacgtc acttttttta cgaaataaac actttaaaca ctacgataac 4020

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... HpaI(4058) .......... ..........

4021 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt 4080

4021 gaaataaaca ttggtaatat tcgacgttat ttgttcaatt gttgttgtta acgtaagtaa 4080

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca 4140

4081 aatacaaagt ccaagtcccc ctctacaccc tccaaaaaat ttcgttcatt ttggagatgt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa gtagcatggc 4200

4141 ttacaccatt ttagctattc ctagaaggat ctcgtaccga tgcatctatt catcgtaccg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc 4260

4201 cccaattagt aattgatgtt ccttggggat cactacctca accggtgagg gagagacgcg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 4320

4261 cgagcgagcg agtgactccg gcccgctggt ttccagcggg ctgcgggccc gaaacgggcc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcggcctcag tgagcgagcg agcgcgcagc cttaattaac ctaattcact ggccgtcgtt 4380

4321 cgccggagtc actcgctcgc tcgcgcgtcg gaattaattg gattaagtga ccggcagcaa 4380

.......... .......... .......... .......... ... lacZ_a M13_forward20_pri

.......... .......... .......... .......... .......... ..........

4381 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 4440

4381 aatgttgcag cactgaccct tttgggaccg caatgggttg aattagcgga acgtcgtgta 4440

M13_pUC_fwd_pri .... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 4500

4441 gggggaaagc ggtcgaccgc attatcgctt ctccgggcgt ggctagcggg aagggttgtc 4500

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt 4560

4501 aacgcgtcgg acttaccgct taccctgcgc gggacatcgc cgcgtaattc gcgccgccca 4560

.......... lacZ_a .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

4561 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 4620

4561 caccaccaat gcgcgtcgca ctggcgatgt gaacggtcgc gggatcgcgg gcgaggaaag 4620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 4680

4621 cgaaagaagg gaaggaaaga gcggtgcaag cggccgaaag gggcagttcg agatttagcc 4680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 4740

4681 cccgagggaa atcccaaggc taaatcacga aatgccgtgg agctggggtt ttttgaacta 4740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 4800

4741 atcccactac caagtgcatc acccggtagc gggactatct gccaaaaagc gggaaactgc 4800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 4860

4801 aacctcaggt gcaagaaatt atcacctgag aacaaggttt gaccttgttg tgagttggga 4860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 4920

4861 tagagccaga taagaaaact aaatattccc taaaacggct aaagccggat aaccaatttt 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 4980

4921 ttactcgact aaattgtttt taaattgcgc ttaaaattgt tttataattg cgaatgttaa 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 5040

4981 atccaccgtg aaaagcccct ttacacgcgc cttggggata aacaaataaa aagatttatg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 5100

5041 taagtttata cataggcgag tactctgtta ttgggactat ttacgaagtt attataactt 5100

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 5160

5101 tttccttctc atactcataa gttgtaaagg cacagcggga ataagggaaa aaacgccgta 5160

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

5161 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 5220

5161 aaacggaagg acaaaaacga gtgggtcttt gcgaccactt tcattttcta cgacttctag 5220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 5280

5221 tcaacccacg tgctcaccca atgtagcttg acctagagtt gtcgccattc taggaactct 5280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 5340

5281 caaaagcggg gcttcttgca aaaggttact actcgtgaaa atttcaagac gatacaccgc 5340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 5400

5341 gccataatag ggcataactg cggcccgttc tcgttgagcc agcggcgtat gtgataagag 5400

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 5460

5401 tcttactgaa ccaactcatg agtggtcagt gtcttttcgt agaatgccta ccgtactgtc 5460

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 5520

5461 attctcttaa tacgtcacga cggtattggt actcactatt gtgacgccgg ttgaatgaag 5520

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 5580

5521 actgttgcta gcctcctggc ttcctcgatt ggcgaaaaaa cgtgttgtac cccctagtac 5580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 5640

5581 attgagcgga actagcaacc cttggcctcg acttacttcg gtatggtttg ctgctcgcac 5640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 5700

5641 tgtggtgcta cggacatcgt taccgttgtt gcaacgcgtt tgataattga ccgcttgatg 5700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 5760

5701 aatgagatcg aagggccgtt gttaattatc tgacctacct ccgcctattt caacgtcctg 5760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 5820

5761 gtgaagacgc gagccgggaa ggccgaccga ccaaataacg actatttaga cctcggccac 5820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 5880

5821 tcgcacccag agcgccatag taacgtcgtg accccggtct accattcggg agggcatagc 5880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 5940

5881 atcaatagat gtgctgcccc tcagtccgtt gatacctact tgctttatct gtctagcgac 5940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 6000

5941 tctatccacg gagtgactaa ttcgtaacca ttgacagtct ggttcaaatg agtatatatg 6000

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 6060

6001 aaatctaact aaattttgaa gtaaaaatta aattttccta gatccacttc taggaaaaac 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 6120

6061 tattagagta ctggttttag ggaattgcac tcaaaagcaa ggtgactcgc agtctggggc 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 6180

6121 atcttttcta gtttcctaga agaactctag gaaaaaaaga cgcgcattag acgacgaacg 6180

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

6181 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 6240

6181 tttgtttttt tggtggcgat ggtcgccacc aaacaaacgg cctagttctc gatggttgag 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 6300

6241 aaaaaggctt ccattgaccg aagtcgtctc gcgtctatgg tttatgacaa gaagatcaca 6300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 6360

6301 tcggcatcaa tccggtggtg aagttcttga gacatcgtgg cggatgtatg gagcgagacg 6360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6361 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 6420

6361 attaggacaa tggtcaccga cgacggtcac cgctattcag cacagaatgg cccaacctga 6420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6421 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 6480

6421 gttctgctat caatggccta ttccgcgtcg ccagcccgac ttgcccccca agcacgtgtg 6480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6481 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 6540

6481 tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat ggatgtcgca ctcgatactc 6540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 6600

6541 tttcgcggtg cgaagggctt ccctctttcc gcctgtccat aggccattcg ccgtcccagc 6600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 6660

6601 cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg gaccatagaa atatcaggac 6660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 6720

6661 agcccaaagc ggtggagact gaactcgcag ctaaaaacac tacgagcagt ccccccgcct 6720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 6780

6721 cggatacctt tttgcggtcg ttgcgccgga aaaatgccaa ggaccggaaa acgaccggaa 6780

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 6840

6781 aacgagtgta caagaaagga cgcaataggg gactaagaca cctattggca taatggcgga 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 6900

6841 aactcactcg actatggcga gcggcgtcgg cttgctggct cgcgtcgctc agtcactcgc 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 aggaagcgga aghelpblas tnblastpro gramsfindr egionsofsi milaritybe 6960

6901 tccttcgcct tcdelpvlts anvltsapyo cytksfinhy ecionsofsi kiltyiarve 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tweenbiolo gicalseque ncesblastn searchesnc bisdnadata basefordna 7020

6961 aweenviolo cigtlseqae ngesvltsan setygdesng vishnthtat vtsefoyhnt 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 sequencess imilartoyo ursequence helpmorese quenceopti onsviewthe 7080

7021 seqaengess ikiltyaoro ayseqaenge delpkoyese qaengeopai onsbiewade 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 sequencein differentf ormatsfind restrictio nsitesview openreadin 7140

7081 seqaengein hiffeyenaf oyktasfinh yesayigaio nsiaesbiew openyethin 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 gframetran slationsan dmorehelpf ultipclick ontheanaly zesequence 7200

7141 cfytkeaytn sltaionstn hkoyedelpf alaipgligm onadetntlr zeseqaenge 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 buttontose erestricti onsitesvie worfsetcad dgenessequ encingresu 7260

7201 vaaaonaose eyesayigai onsiaesbie woyfseagth hcenesseqa engincyesa 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 lts....... .......... .......... .......... .......... .......... 7263

7261 las....... .......... .......... .......... .......... .......... 7263

.......... .......... .......... .......... .......... ..........