Sequence of pAED4

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Sequence of pAED4

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Sequence of pAED4 : 3322 BP; 882 A; 805 C; 841 G; 794 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ggggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc 60

1 cccctagagc tagggcgctt taattatgct gagtgatatc cctctggtgt tgccaaaggg 60

.......... .......... .......... T7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctagaaata attttgttta actttaagaa ggagatatac atatggacat attgtcgtta 120

61 agatctttat taaaacaaat tgaaattctt cctctatatg tatacctgta taacagcaat 120

.......... ....... T7_transl_en_RBS ....... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac 180

121 cttgcgccga tgttaattat gtattggaat acatagtatg tgtatgctaa atccactgtg 180

.......... .......... .......... .......... .......... Sp6_pri

XhoI PvuII(196),HindIII(200) PstI(216) BamHI(230) ......

181 tatagaactc gagcagctga agcttgcatg cctgcaggtc gactctagag gatccccggg 240

181 atatcttgag ctcgtcgact tcgaacgtac ggacgtccag ctgagatctc ctaggggccc 240

.......... .......... .......... .......... .......... ..........

KpnI(243) SacI(249) EcoRI(251) ClaI(258) .......... ..........

241 taccgagctc gaattcatcg atgatatcag atccggctgc taacaaagcc cgaaaggaag 300

241 atggctcgag cttaagtagc tactatagtc taggccgacg attgtttcgg gctttccttc 300

.......... .......... .......... .......... T7_term ..........

.......... .......... .......... .......... .......... ..........

301 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac 360

301 gactcaaccg acgacggtgg cgactcgtta ttgatcgtat tggggaaccc cggagatttg 360

.......... ... T7_term T7_Terminal_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatcggat atcacaggac 420

361 cccagaactc cccaaaaaac gactttcctc cttgatatag gcctagccta tagtgtcctg 420

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac 480

421 cccacaccag cggtactagc gcatcagcta tcaccgaggt tcatcgcttc gctcgtcctg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat 540

481 acccgccgcc ggtttcgcca gcctgtcacg aggctcttgc ccacgcgtat ctttaacgta 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caacgcatat agcgctagag tcgacctgca ttaatgaatc ggccaacgcg cggggagagg 600

541 gttgcgtata tcgcgatctc agctggacgt aattacttag ccggttgcgc gcccctctcc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 660

601 gccaaacgca taacccgcga gaaggcgaag gagcgagtga ctgagcgacg cgagccagca 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 720

661 agccgacgcc gctcgccata gtcgagtgag tttccgccat tatgccaata ggtgtcttag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 780

721 tcccctattg cgtcctttct tgtacactcg ttttccggtc gttttccggt ccttggcatt 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 840

781 tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg ggactgctcg tagtgttttt 840

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 900

841 agctgcgagt tcagtctcca ccgctttggg ctgtcctgat atttctatgg tccgcaaagg 900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 960

901 gggaccttcg agggagcacg cgagaggaca aggctgggac ggcgaatggc ctatggacag 960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag 1020

961 gcggaaagag ggaagccctt cgcaccgcga aagagttacg agtgcgacat ccatagagtc 1020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 1080

1021 aagccacatc cagcaagcga ggttcgaccc gacacacgtg cttggggggc aagtcgggct 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 1140

1081 ggcgacgcgg aataggccat tgatagcaga actcaggttg ggccattctg tgctgaatag 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 1200

1141 cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc tccatacatc cgccacgatg 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 1260

1201 tctcaagaac ttcaccaccg gattgatgcc gatgtgatct tcctgtcata aaccatagac 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 1320

1261 gcgagacgac ttcggtcaat ggaagccttt ttctcaacca tcgagaacta ggccgtttgt 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 1380

1321 ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc gtctaatgcg cgtctttttt 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 1440

1381 tcctagagtt cttctaggaa actagaaaag atgccccaga ctgcgagtca ccttgctttt 1440

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 1500

1441 gagtgcaatt ccctaaaacc agtactctaa tagtttttcc tagaagtgga tctaggaaaa 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 1560

1501 tttaattttt acttcaaaat ttagttagat ttcatatata ctcatttgaa ccagactgtc 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 1620

1561 aatggttacg aattagtcac tccgtggata gagtcgctag acagataaag caagtaggta 1620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 1680

1621 tcaacggact gaggggcagc acatctattg atgctatgcc ctcccgaatg gtagaccggg 1680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 1740

1681 gtcacgacgt tactatggcg ctctgggtgc gagtggccga ggtctaaata gtcgttattt 1740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 1800

1741 ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt tgaaataggc ggaggtaggt 1800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 1860

1801 cagataatta acaacggccc ttcgatctca ttcatcaagc ggtcaattat caaacgcgtt 1860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 1920

1861 gcaacaacgg taacgatgtc cgtagcacca cagtgcgagc agcaaaccat accgaagtaa 1920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 1980

1921 gtcgaggcca agggttgcta gttccgctca atgtactagg gggtacaaca cgttttttcg 1980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 2040

1981 ccaatcgagg aagccaggag gctagcaaca gtcttcattc aaccggcgtc acaatagtga 2040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 2100

2041 gtaccaatac cgtcgtgacg tattaagaga atgacagtac ggtaggcatt ctacgaaaag 2100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 2160

2101 acactgacca ctcatgagtt ggttcagtaa gactcttatc acatacgccg ctggctcaac 2160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 2220

2161 gagaacgggc cgcagttatg ccctattatg gcgcggtgta tcgtcttgaa attttcacga 2220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 2280

2221 gtagtaacct tttgcaagaa gccccgcttt tgagagttcc tagaatggcg acaactctag 2280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 2340

2281 gtcaagctac attgggtgag cacgtgggtt gactagaagt cgtagaaaat gaaagtggtc 2340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 2400

2341 gcaaagaccc actcgttttt gtccttccgt tttacggcgt tttttccctt attcccgctg 2400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 2460

2401 tgcctttaca acttatgagt atgagaagga aaaagttata ataacttcgt aaatagtccc 2460

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 2520

2461 aataacagag tactcgccta tgtataaact tacataaatc tttttatttg tttatcccca 2520

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... ......... AatII(2556) ......... ..........

2521 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 2580

2521 aggcgcgtgt aaaggggctt ttcacggtgg actgcagatt ctttggtaat aatagtactg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 2640

2581 taattggata tttttatccg catagtgctc cgggaaagca gagcgcgcaa agccactact 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 2700

2641 gccacttttg gagactgtgt acgtcgaggg cctctgccag tgtcgaacag acattcgcct 2700

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 2760

2701 acggccctcg tctgttcggg cagtcccgcg cagtcgccca caaccgccca cagccccgac 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcttaactat gcggcatcag agcagattgt actgagagtg cagtacccac cccggttgat 2820

2761 cgaattgata cgccgtagtc tcgtctaaca tgactctcac gtcatgggtg gggccaacta 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaaacgtt 2880

2821 ttagtctttt cggggttttt gtccttctaa catattcgtt tataaattta acatttgcaa 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 2940

2881 ttataaaaca attttaagcg caatttaaaa acaatttagt cgagtaaaaa attggttatc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 3000

2941 cggctttagc cgttttaggg aatatttagt tttcttatct ggctctatcc caactcacaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 3060

3001 caaggtcaaa ccttgttctc aggtgataat ttcttgcacc tgaggttgca gtttcccgct 3060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 3120

3061 ttttggcaga tagtcccgct accgggtgat gcacttggta gtgggattag ttcaaaaaac 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 3180

3121 cccagctcca cggcatttcg tgatttagcc ttgggatttc cctcgggggc taaatctcga 3180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 3240

3181 actgcccctt tcggccgctt gcaccgctct ttccttccct tctttcgctt tcctcgcccg 3240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 3300

3241 cgatcccgcg accgttcaca tcgccagtgc gacgcgcatt ggtggtgtgg gcggcgcgaa 3300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aatgcgccgc tacagggcgc gt........ .......... .......... .......... 3322

3301 ttacgcggcg atgtcccgcg ca........ .......... .......... .......... 3322

.......... .......... .......... .......... .......... ..........