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    Plasmid map of pAG143_AK024929

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    pAG143 AK024929 3'UTR
    Gene/insert name: N8 3'UTR
    Insert size (bp): 900
    GenBank/Entrez ID of insert: AK024929
    Species of gene(s): H. sapiens (human)
    Vector backbone: pIS2 (Search Vector Database)
    Backbone manufacturer: pIS2 derived from pRL-SV40 (Promega)
    Type of vector: Mammalian expression,Luciferase
    Backbone size (bp): 4619
    Cloning site 5': SacI
    Site destroyed during cloning: No
    Cloning site 3': SpeI
    Site destroyed during cloning: No
    5' Sequencing primer: EBV rev primer (GTGGTTTGTCCAAACTCATC) (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a
    Principal Investigator: David Bartel
    Comments: AK024929 3'UTR in renilla luciferase reporter plasmid. Wild-type microRNA recognition sites (seed matches): 1834-1839 and 2380-2385.
    Article: The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Farh KK et al. (Science. 2005 Dec 16. 310(5755):1817-21. Pubmed)

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