Sequence of pAG143

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Sequence of pAG143_AK024929

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Sequence of pAG143 AK024929 3'UTR : 4619 BP; 1257 A; 993 C; 1091 G; 1278 T; 0 other;

BglII(1) .......... .......... .......... .......... ..........

1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 60

1 tctagacgcg tcgtggtacc ggactttatt ggagactttc tccttgaacc aatccatgga 60

.......... .......... .......... .......... .......... ..........

.......... PvuII(80) .......... .......... .......... ..........

61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 120

61 agactccgcc tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc 120

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 180

121 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 180

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 240

181 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 240

.......... ...... SV40_enh SV40_pro ...... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 300

241 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 300

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 360

301 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc 360

........ SV40_pro SV40_pro SV40_origin SV40pro_F_pri ........

.......... .......... .......... .......... .......... ..........

361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 420

361 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt 420

SV40_pro .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... PstI(462) ..........

421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 480

421 tcgaactaag aagactgtgt tgtcagagct tgaattcgac gtcttcaacc agcactccgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 540

481 gacccgtcca ttcatagttc caatgttctg tccaaattcc tctggttatc tttgacccga 540

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 600

541 acagctctgt ctcttctgag aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg 600

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 660

601 tgaaacggaa agagaggtgt ccacaggtga gggtcaagtt aatgtcgaga attccgatct 660

.......... chim_int .......... .......... .......... ..........

.......... .......... NheI(684) BstBI(700) .......... ..........

661 gtacttaata cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa 720

661 catgaattat gctgagtgat atccgatcgg tggtactgaa gctttcaaat actaggtctt 720

.......... T7_pro .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

721 caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt 780

721 gtttcctttg cctactattg accaggcgtc accacccggt ctacatttgt ttacttacaa 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt 840

781 gaactaagta aataattaat aatactaagt ctttttgtac gtcttttacg acaataaaaa 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca 900

841 aatgtaccat tgcgccggag aagaataaat accgctgtac aacacggtgt ataactcggt 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat 960

901 catcgcgcca cataatatgg tctggaataa ccatacccgt ttagtccgtt tagaccatta 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat 1020

961 ccaagaatat ccaatgaact agtaatgttt atagaatgac gtaccaaact tgaagaatta 1020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat 1080

1021 aatggtttct tctagtaaaa acagccggta ctaaccccac gaacaaaccg taaagtaata 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg 1140

1081 tcgatactcg tagttctatt ctagtttcgt tatcaagtgc gactttcaca tcatctacac 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa 1200

1141 taacttagta ccctacttac cggactataa cttcttctat aacgcaacta gtttagactt 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa 1260

1201 cttcctcttt tttaccaaaa cctcttattg aagaagcacc tttggtacaa cggtagtttt 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt caaagagaaa 1320

1261 tagtactctt tcaatcttgg tcttcttaaa cgtcgtatag aacttggtaa gtttctcttt 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt 1380

1321 ccacttcaag cagcaggttg taatagtacc ggagcacttt agggcaatca ttttccacca 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc aagtgatgat 1440

1381 tttggactgc aacatgttta acaatcctta atattacgaa tagatgcacg ttcactacta 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat tgttgaaggt 1500

1441 aatggttttt acaaataact tagcctgggt cctaagaaaa ggttacgata acaacttcca 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa 1560

1501 cggttcttca aaggattatg acttaaacag tttcattttc cagaagtaaa aagcgttctt 1560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat 1620

1561 ctacgtggac tactttaccc ttttatatag tttagcaagc aactcgctca agagttttta 1620

.......... .......... ORF 1 .......... .......... ..........

.......... SacI(1640) .......... .......... .......... ..........

1621 gaacaataat tctaggagct ccgatgccct ctttttctca ggatagcaga taacctgctt 1680

1621 cttgttatta agatcctcga ggctacggga gaaaaagagt cctatcgtct attggacgaa 1680

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgaaagaggg cttaattctg tgggtcctaa attttctcct ttctctctct ctttctgtgt 1740

1681 actttctccc gaattaagac acccaggatt taaaagagga aagagagaga gaaagacaca 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtgtgtgttg ggaaaatggc aagtttccaa taccagcttt ggaggaacga ttacgttttc 1800

1741 cacacacaac ccttttaccg ttcaaaggtt atggtcgaaa cctccttgct aatgcaaaag 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cctccaattt caagtccgaa agaccagagc cctcattcca aagcccccca cccagatgga 1860

1801 ggaggttaaa gttcaggctt tctggtctcg ggagtaaggt ttcggggggt gggtctacct 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ApaI(1899) .......... ..........

1861 ttttttcgtt tcatttgtca tccgtcccat gggagggccc catgtctcct cagaacccat 1920

1861 aaaaaagcaa agtaaacagt aggcagggta ccctcccggg gtacagagga gtcttgggta 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cctggaggca gcaggtcggg tagagtgagt ttggcctgct catgacctcc acccctgaga 1980

1921 ggacctccgt cgtccagccc atctcactca aaccggacga gtactggagg tggggactct 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttgtgaacaa ggatgtctgg ggcgatgctg agaatgtttt tgaagctgct cccagatgac 2040

1981 aacacttgtt cctacagacc ccgctacgac tcttacaaaa acttcgacga gggtctactg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gctgatgatc acaccagatt gagtgctgcg atcgccttga gtccaacctc tgcataaacg 2100

2041 cgactactag tgtggtctaa ctcacgacgc tagcggaact caggttggag acgtatttgc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aggttctcat aaacaagttc actctaccct aagctaagtc tatgtgagca aacccacttc 2160

2101 tccaagagta tttgttcaag tgagatggga ttcgattcag atacactcgt ttgggtgaag 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 atcctttgta cctggagacc tggttacact aacctgatac tgacctgttc atgtagctgg 2220

2161 taggaaacat ggacctctgg accaatgtga ttggactatg actggacaag tacatcgacc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aatggtgtgt ttcatgcagt gtggaccaag caatggcatg gggtgtgtgt gtgtgtgtgt 2280

2221 ttaccacaca aagtacgtca cacctggttc gttaccgtac cccacacaca cacacacaca 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gtgtgtctgt gtgtgtgtgt ttgtgtatgc gttcacactt gtgtgtgtat atgtgcatgt 2340

2281 cacacagaca cacacacaca aacacatacg caagtgtgaa cacacacata tacacgtaca 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agatgctgca taaatgattt ttgatgtcaa agacaaacac attccattgt tttaaatatt 2400

2341 tctacgacgt atttactaaa aactacagtt tctgtttgtg taaggtaaca aaatttataa 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctattatgta aacaatacgc agagggacca tatctactct tgtcatatta tttgtgatgg 2460

2401 gataatacat ttgttatgcg tctccctggt atagatgaga acagtataat aaacactacc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 taaaacatgc atttgcaata aattaagctt tctgggaagg caagcagtat tggagccaaa 2520

2461 attttgtacg taaacgttat ttaattcgaa agacccttcc gttcgtcata acctcggttt 2520

.......... .......... .......... .......... .......... ..........

.... SpeI(2537) XbaI(2545) NotI(2552),EagI(2552) .... ..........

2521 cgactgtctc ggaacaacta gtgttctaga gcggccgctt cgagcagaca tgataagata 2580

2521 gctgacagag ccttgttgat cacaagatct cgccggcgaa gctcgtctgt actattctat 2580

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2581 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 2640

2581 gtaactactc aaacctgttt ggtgttgatc ttacgtcact ttttttacga aataaacact 2640

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 2700

2641 ttaaacacta cgataacgaa ataaacattg gtaatattcg acgttatttg ttcaattgtt 2700

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 2760

2701 gttgttaacg taagtaaaat acaaagtcca agtccccctc cacaccctcc aaaaaatttc 2760

.......... .......... .......... .......... .......... ..........

.......... ......... ClaI(2790) BamHI(2797) ......... ..........

2761 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccaggtggca cttttcgggg 2820

2761 gttcattttg gagatgttta caccatttta gctattccta ggtccaccgt gaaaagcccc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 2880

2821 tttacacgcg ccttggggat aaacaaataa aaagatttat gtaagtttat acataggcga 2880

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

2881 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 2940

2881 gtactctgtt attgggacta tttacgaagt tattataact ttttccttct catactcata 2940

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 3000

2941 agttgtaaag gcacagcggg aataagggaa aaaacgccgt aaaacggaag gacaaaaacg 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 3060

3001 agtgggtctt tgcgaccact ttcattttct acgacttcta gtcaacccac gtgctcaccc 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 3120

3061 aatgtagctt gacctagagt tgtcgccatt ctaggaactc tcaaaagcgg ggcttcttgc 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 3180

3121 aaaaggttac tactcgtgaa aatttcaaga cgatacaccg cgccataata gggcataact 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 3240

3181 gcggcccgtt ctcgttgagc cagcggcgta tgtgataaga gtcttactga accaactcat 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 3300

3241 gagtggtcag tgtcttttcg tagaatgcct accgtactgt cattctctta atacgtcacg 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 3360

3301 acggtattgg tactcactat tgtgacgccg gttgaatgaa gactgttgct agcctcctgg 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 3420

3361 cttcctcgat tggcgaaaaa acgtgttgta ccccctagta cattgagcgg aactagcaac 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 3480

3421 ccttggcctc gacttacttc ggtatggttt gctgctcgca ctgtggtgct acggacatcg 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 3540

3481 ttaccgttgt tgcaacgcgt ttgataattg accgcttgat gaatgagatc gaagggccgt 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 3600

3541 tgttaattat ctgacctacc tccgcctatt tcaacgtcct ggtgaagacg cgagccggga 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 3660

3601 aggccgaccg accaaataac gactatttag acctcggcca ctcgcaccca gagcgccata 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 3720

3661 gtaacgtcgt gaccccggtc taccattcgg gagggcatag catcaataga tgtgctgccc 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 3780

3721 ctcagtccgt tgatacctac ttgctttatc tgtctagcga ctctatccac ggagtgacta 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 3840

3781 attcgtaacc attgacagtc tggttcaaat gagtatatat gaaatctaac taaattttga 3840

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 3900

3841 agtaaaaatt aaattttcct agatccactt ctaggaaaaa ctattagagt actggtttta 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 3960

3901 gggaattgca ctcaaaagca aggtgactcg cagtctgggg catcttttct agtttcctag 3960

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3961 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4020

3961 aagaactcta ggaaaaaaag acgcgcatta gacgacgaac gtttgttttt ttggtggcga 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4080

4021 tggtcgccac caaacaaacg gcctagttct cgatggttga gaaaaaggct tccattgacc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 4140

4081 gaagtcgtct cgcgtctatg gtttatgaca agaagatcac atcggcatca atccggtggt 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4200

4141 gaagttcttg agacatcgtg gcggatgtat ggagcgagac gattaggaca atggtcaccg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4260

4201 acgacggtca ccgctattca gcacagaatg gcccaacctg agttctgcta tcaatggcct 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4320

4261 attccgcgtc gccagcccga cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4380

4321 ctggatgtgg cttgactcta tggatgtcgc actcgatact ctttcgcggt gcgaagggct 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4440

4381 tccctctttc cgcctgtcca taggccattc gccgtcccag ccttgtcctc tcgcgtgctc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4500

4441 cctcgaaggt ccccctttgc ggaccataga aatatcagga cagcccaaag cggtggagac 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4560

4501 tgaactcgca gctaaaaaca ctacgagcag tccccccgcc tcggatacct ttttgcggtc 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tggctcgac. 4619

4561 gttgcgccgg aaaaatgcca aggaccggaa aacgaccgga aaacgagtgt accgagctg. 4619

.......... .......... .......... .......... .......... ..........