Sequence of pAG147

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Sequence of pAG147_BC072452

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Sequence of pAG147 BC072452 3'UTR : 4452 BP; 1250 A; 939 C; 1029 G; 1234 T; 0 other;

BglII(1) .......... .......... .......... .......... ..........

1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 60

1 tctagacgcg tcgtggtacc ggactttatt ggagactttc tccttgaacc aatccatgga 60

.......... .......... .......... .......... .......... ..........

.......... PvuII(80) .......... .......... .......... ..........

61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 120

61 agactccgcc tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc 120

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 180

121 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 180

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 240

181 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 240

.......... ...... SV40_enh SV40_pro ...... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 300

241 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 300

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 360

301 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc 360

........ SV40_pro SV40_pro SV40_origin SV40pro_F_pri ........

.......... .......... .......... .......... StuI(403) ..........

361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 420

361 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt 420

SV40_pro .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... PstI(462) ..........

421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 480

421 tcgaactaag aagactgtgt tgtcagagct tgaattcgac gtcttcaacc agcactccgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 540

481 gacccgtcca ttcatagttc caatgttctg tccaaattcc tctggttatc tttgacccga 540

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 600

541 acagctctgt ctcttctgag aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg 600

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 660

601 tgaaacggaa agagaggtgt ccacaggtga gggtcaagtt aatgtcgaga attccgatct 660

.......... chim_int .......... .......... .......... ..........

.......... .......... NheI(684) BstBI(700) .......... ..........

661 gtacttaata cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa 720

661 catgaattat gctgagtgat atccgatcgg tggtactgaa gctttcaaat actaggtctt 720

.......... T7_pro .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

721 caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt 780

721 gtttcctttg cctactattg accaggcgtc accacccggt ctacatttgt ttacttacaa 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt 840

781 gaactaagta aataattaat aatactaagt ctttttgtac gtcttttacg acaataaaaa 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca 900

841 aatgtaccat tgcgccggag aagaataaat accgctgtac aacacggtgt ataactcggt 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat 960

901 catcgcgcca cataatatgg tctggaataa ccatacccgt ttagtccgtt tagaccatta 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat 1020

961 ccaagaatat ccaatgaact agtaatgttt atagaatgac gtaccaaact tgaagaatta 1020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat 1080

1021 aatggtttct tctagtaaaa acagccggta ctaaccccac gaacaaaccg taaagtaata 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg 1140

1081 tcgatactcg tagttctatt ctagtttcgt tatcaagtgc gactttcaca tcatctacac 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa 1200

1141 taacttagta ccctacttac cggactataa cttcttctat aacgcaacta gtttagactt 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa 1260

1201 cttcctcttt tttaccaaaa cctcttattg aagaagcacc tttggtacaa cggtagtttt 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt caaagagaaa 1320

1261 tagtactctt tcaatcttgg tcttcttaaa cgtcgtatag aacttggtaa gtttctcttt 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt 1380

1321 ccacttcaag cagcaggttg taatagtacc ggagcacttt agggcaatca ttttccacca 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc aagtgatgat 1440

1381 tttggactgc aacatgttta acaatcctta atattacgaa tagatgcacg ttcactacta 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat tgttgaaggt 1500

1441 aatggttttt acaaataact tagcctgggt cctaagaaaa ggttacgata acaacttcca 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa 1560

1501 cggttcttca aaggattatg acttaaacag tttcattttc cagaagtaaa aagcgttctt 1560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat 1620

1561 ctacgtggac tactttaccc ttttatatag tttagcaagc aactcgctca agagttttta 1620

.......... .......... ORF 1 .......... .......... ..........

.......... SacI(1640) .......... .......... .......... ..........

1621 gaacaataat tctaggagct ctcctattcg ccaaatgaag ggcagtgccc cgcacgtaag 1680

1621 cttgttatta agatcctcga gaggataagc ggtttacttc ccgtcacggg gcgtgcattc 1680

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttggaatgat ggacctgtgt tcagagactt aacagaacca acaagcaaaa caagtgagaa 1740

1681 aaccttacta cctggacaca agtctctgaa ttgtcttggt tgttcgtttt gttcactctt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 caggaaaaag gaagaggaca ctggaatcaa ttcttgagag ttgcactact tggtttttct 1800

1741 gtcctttttc cttctcctgt gaccttagtt aagaactctc aacgtgatga accaaaaaga 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tccattccaa gtttcgtggg acccagagcc ttttttcttt taaaagctaa aaaacaagtg 1860

1801 aggtaaggtt caaagcaccc tgggtctcgg aaaaaagaaa attttcgatt ttttgttcac 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tttaattcct ctttttgtta tctgttagat aattgagatc acctagaaat gcgtttaatc 1920

1861 aaattaagga gaaaaacaat agacaatcta ttaactctag tggatcttta cgcaaattag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgttcactca ctgtaaattt tgaggaccca gaattgtctt gtttaattta tactttcacc 1980

1921 acaagtgagt gacatttaaa actcctgggt cttaacagaa caaattaaat atgaaagtgg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cctgttgcag ttaacaccag agaaggaacg tgaatgtcga gcacagccac tacccttgtt 2040

1981 ggacaacgtc aattgtggtc tcttccttgc acttacagct cgtgtcggtg atgggaacaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggcacttaat ttagaaatag ggtgagaagt ttaaaagccc atcttgattt tattttcatt 2100

2041 ccgtgaatta aatctttatc ccactcttca aattttcggg tagaactaaa ataaaagtaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccttttggtt ctctgtgtaa taatagcagg ctacatagtg acattccagt tccaagaagg 2160

2101 ggaaaaccaa gagacacatt attatcgtcc gatgtatcac tgtaaggtca aggttcttcc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tacatcctgt ccattcatta attgctttga ttactaggag ggtttctgtt cagttttgtt 2220

2161 atgtaggaca ggtaagtaat taacgaaact aatgatcctc ccaaagacaa gtcaaaacaa 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tttaaatgtc ttgctgatct agttctttca gatggaataa ccttccagtc ccttagagag 2280

2221 aaatttacag aacgactaga tcaagaaagt ctaccttatt ggaaggtcag ggaatctctc 2280

.......... .......... .......... .......... .......... ..........

SpeI(2285) .......... .......... .......... .......... ..........

2281 tggaactagt ccatataacc cagcttcagt agcaaaagta gaagccgcca catcttttca 2340

2281 accttgatca ggtatattgg gtcgaagtca tcgttttcat cttcggcggt gtagaaaagt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .... XbaI(2378) NotI(2385),EagI(2385) ....

2341 tttctccaag aggagagtgg ggaaggttcg ctagtgttct agagcggccg cttcgagcag 2400

2341 aaagaggttc tcctctcacc ccttccaagc gatcacaaga tctcgccggc gaagctcgtc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 2460

2401 tgtactattc tatgtaacta ctcaaacctg tttggtgttg atcttacgtc acttttttta 2460

.......... ......... EBV_r SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

2461 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 2520

2461 cgaaataaac actttaaaca ctacgataac gaaataaaca ttggtaatat tcgacgttat 2520

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 2580

2521 ttgttcaatt gttgttgtta acgtaagtaa aatacaaagt ccaagtcccc ctccacaccc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .... ClaI(2623) BamHI(2630) ....

2581 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccaggtg 2640

2581 tccaaaaaat ttcgttcatt ttggagatgt ttacaccatt ttagctattc ctaggtccac 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 2700

2641 cgtgaaaagc ccctttacac gcgccttggg gataaacaaa taaaaagatt tatgtaagtt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 2760

2701 tatacatagg cgagtactct gttattggga ctatttacga agttattata actttttcct 2760

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 2820

2761 tctcatactc ataagttgta aaggcacagc gggaataagg gaaaaaacgc cgtaaaacgg 2820

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 2880

2821 aaggacaaaa acgagtgggt ctttgcgacc actttcattt tctacgactt ctagtcaacc 2880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 2940

2881 cacgtgctca cccaatgtag cttgacctag agttgtcgcc attctaggaa ctctcaaaag 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 3000

2941 cggggcttct tgcaaaaggt tactactcgt gaaaatttca agacgataca ccgcgccata 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 3060

3001 atagggcata actgcggccc gttctcgttg agccagcggc gtatgtgata agagtcttac 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 3120

3061 tgaaccaact catgagtggt cagtgtcttt tcgtagaatg cctaccgtac tgtcattctc 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 3180

3121 ttaatacgtc acgacggtat tggtactcac tattgtgacg ccggttgaat gaagactgtt 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 3240

3181 gctagcctcc tggcttcctc gattggcgaa aaaacgtgtt gtacccccta gtacattgag 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 3300

3241 cggaactagc aacccttggc ctcgacttac ttcggtatgg tttgctgctc gcactgtggt 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 3360

3301 gctacggaca tcgttaccgt tgttgcaacg cgtttgataa ttgaccgctt gatgaatgag 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 3420

3361 atcgaagggc cgttgttaat tatctgacct acctccgcct atttcaacgt cctggtgaag 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 3480

3421 acgcgagccg ggaaggccga ccgaccaaat aacgactatt tagacctcgg ccactcgcac 3480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 3540

3481 ccagagcgcc atagtaacgt cgtgaccccg gtctaccatt cgggagggca tagcatcaat 3540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 3600

3541 agatgtgctg cccctcagtc cgttgatacc tacttgcttt atctgtctag cgactctatc 3600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 3660

3601 cacggagtga ctaattcgta accattgaca gtctggttca aatgagtata tatgaaatct 3660

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 3720

3661 aactaaattt tgaagtaaaa attaaatttt cctagatcca cttctaggaa aaactattag 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 3780

3721 agtactggtt ttagggaatt gcactcaaaa gcaaggtgac tcgcagtctg gggcatcttt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 3840

3781 tctagtttcc tagaagaact ctaggaaaaa aagacgcgca ttagacgacg aacgtttgtt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 3900

3841 tttttggtgg cgatggtcgc caccaaacaa acggcctagt tctcgatggt tgagaaaaag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 3960

3901 gcttccattg accgaagtcg tctcgcgtct atggtttatg acaagaagat cacatcggca 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 4020

3961 tcaatccggt ggtgaagttc ttgagacatc gtggcggatg tatggagcga gacgattagg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 4080

4021 acaatggtca ccgacgacgg tcaccgctat tcagcacaga atggcccaac ctgagttctg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 4140

4081 ctatcaatgg cctattccgc gtcgccagcc cgacttgccc cccaagcacg tgtgtcgggt 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 4200

4141 cgaacctcgc ttgctggatg tggcttgact ctatggatgt cgcactcgat actctttcgc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 4260

4201 ggtgcgaagg gcttccctct ttccgcctgt ccataggcca ttcgccgtcc cagccttgtc 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 4320

4261 ctctcgcgtg ctccctcgaa ggtccccctt tgcggaccat agaaatatca ggacagccca 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 4380

4321 aagcggtgga gactgaactc gcagctaaaa acactacgag cagtcccccc gcctcggata 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 4440

4381 cctttttgcg gtcgttgcgc cggaaaaatg ccaaggaccg gaaaacgacc ggaaaacgag 4440

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 acatggctcg ac........ .......... .......... .......... .......... 4452

4441 tgtaccgagc tg........ .......... .......... .......... .......... 4452

.......... .......... .......... .......... .......... ..........