Sequence of pAG184

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Sequence of pAG184_NEK6

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Sequence of pAG184 NEK6 3'UTR : 4096 BP; 1129 A; 892 C; 984 G; 1091 T; 0 other;

BglII(1) .......... .......... .......... .......... KpnI(58)

1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 60

1 tctagacgcg tcgtggtacc ggactttatt ggagactttc tccttgaacc aatccatgga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 120

61 agactccgcc tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc 120

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 180

121 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 180

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 240

181 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 240

.......... ...... SV40_enh SV40_pro ...... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 300

241 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 300

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 360

301 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc 360

........ SV40_pro SV40_pro SV40_origin SV40pro_F_pri ........

.......... .......... .......... .......... StuI(403) ..........

361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 420

361 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt 420

SV40_pro .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... PstI(462) ..........

421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 480

421 tcgaactaag aagactgtgt tgtcagagct tgaattcgac gtcttcaacc agcactccgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 540

481 gacccgtcca ttcatagttc caatgttctg tccaaattcc tctggttatc tttgacccga 540

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 600

541 acagctctgt ctcttctgag aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg 600

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 660

601 tgaaacggaa agagaggtgt ccacaggtga gggtcaagtt aatgtcgaga attccgatct 660

.......... chim_int .......... .......... .......... ..........

.......... .......... NheI(684) BstBI(700) .......... ..........

661 gtacttaata cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa 720

661 catgaattat gctgagtgat atccgatcgg tggtactgaa gctttcaaat actaggtctt 720

.......... T7_pro .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

721 caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt 780

721 gtttcctttg cctactattg accaggcgtc accacccggt ctacatttgt ttacttacaa 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt 840

781 gaactaagta aataattaat aatactaagt ctttttgtac gtcttttacg acaataaaaa 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca 900

841 aatgtaccat tgcgccggag aagaataaat accgctgtac aacacggtgt ataactcggt 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat 960

901 catcgcgcca cataatatgg tctggaataa ccatacccgt ttagtccgtt tagaccatta 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat 1020

961 ccaagaatat ccaatgaact agtaatgttt atagaatgac gtaccaaact tgaagaatta 1020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat 1080

1021 aatggtttct tctagtaaaa acagccggta ctaaccccac gaacaaaccg taaagtaata 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg 1140

1081 tcgatactcg tagttctatt ctagtttcgt tatcaagtgc gactttcaca tcatctacac 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa 1200

1141 taacttagta ccctacttac cggactataa cttcttctat aacgcaacta gtttagactt 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa 1260

1201 cttcctcttt tttaccaaaa cctcttattg aagaagcacc tttggtacaa cggtagtttt 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt caaagagaaa 1320

1261 tagtactctt tcaatcttgg tcttcttaaa cgtcgtatag aacttggtaa gtttctcttt 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt 1380

1321 ccacttcaag cagcaggttg taatagtacc ggagcacttt agggcaatca ttttccacca 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc aagtgatgat 1440

1381 tttggactgc aacatgttta acaatcctta atattacgaa tagatgcacg ttcactacta 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat tgttgaaggt 1500

1441 aatggttttt acaaataact tagcctgggt cctaagaaaa ggttacgata acaacttcca 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa 1560

1501 cggttcttca aaggattatg acttaaacag tttcattttc cagaagtaaa aagcgttctt 1560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat 1620

1561 ctacgtggac tactttaccc ttttatatag tttagcaagc aactcgctca agagttttta 1620

.......... .......... ORF 1 .......... .......... ..........

.......... SacI(1640) .......... .......... .......... ..........

1621 gaacaataat tctaggagct cgcgtggatg caccgtgcct tatcaaagcc agcaccactt 1680

1621 cttgttatta agatcctcga gcgcacctac gtggcacgga atagtttcgg tcgtggtgaa 1680

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgccttactt gagtcgtctt ctcttcgagt ggccacctgg tagcctagaa cagctaagac 1740

1681 acggaatgaa ctcagcagaa gagaagctca ccggtggacc atcggatctt gtcgattctg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cacagggttc agcaggttcc ccaaaaggct gcccagcctt acagcagatg ctgaaggcag 1800

1741 gtgtcccaag tcgtccaagg ggttttccga cgggtcggaa tgtcgtctac gacttccgtc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agcagctgag ggaggggcgc tggccacatg tcactgatgg tcagattcca aagtcctttc 1860

1801 tcgtcgactc cctccccgcg accggtgtac agtgactacc agtctaaggt ttcaggaaag 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tttatactgt tgtggacaat ctcagctggg tcaataaggg caggtggttc agcgagccac 1920

1861 aaatatgaca acacctgtta gagtcgaccc agttattccc gtccaccaag tcgctcggtg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggcagccccc tgtatctgga ttgtaatgtg aatctttagg gtaattcctc cagtgacctg 1980

1921 ccgtcggggg acatagacct aacattacac ttagaaatcc cattaaggag gtcactggac 1980

.......... .......... .......... .......... .......... ..........

.......... ......... SpeI(2014) XbaI(2022) NotI(2029),EagI(2029)

1981 tcaaggctta tgctaacagg agacttgcag gagactagtg ttctagagcg gccgcttcga 2040

1981 agttccgaat acgattgtcc tctgaacgtc ctctgatcac aagatctcgc cggcgaagct 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa 2100

2041 cgtctgtact attctatgta actactcaaa cctgtttggt gttgatctta cgtcactttt 2100

.......... .......... ... SV40_PA_term EBV_rev_pri ... ..........

.......... .......... .......... .......... .......... ..........

2101 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc 2160

2101 tttacgaaat aaacacttta aacactacga taacgaaata aacattggta atattcgacg 2160

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg 2220

2161 ttatttgttc aattgttgtt gttaacgtaa gtaaaataca aagtccaagt ccccctccac 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... ClaI(2267) BamHI(2274)

2221 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca 2280

2221 accctccaaa aaatttcgtt cattttggag atgtttacac cattttagct attcctaggt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 2340

2281 ccaccgtgaa aagccccttt acacgcgcct tggggataaa caaataaaaa gatttatgta 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 2400

2341 agtttataca taggcgagta ctctgttatt gggactattt acgaagttat tataactttt 2400

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 2460

2401 tccttctcat actcataagt tgtaaaggca cagcgggaat aagggaaaaa acgccgtaaa 2460

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2461 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 2520

2461 acggaaggac aaaaacgagt gggtctttgc gaccactttc attttctacg acttctagtc 2520

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 2580

2521 aacccacgtg ctcacccaat gtagcttgac ctagagttgt cgccattcta ggaactctca 2580

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 2640

2581 aaagcggggc ttcttgcaaa aggttactac tcgtgaaaat ttcaagacga tacaccgcgc 2640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 2700

2641 cataataggg cataactgcg gcccgttctc gttgagccag cggcgtatgt gataagagtc 2700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 2760

2701 ttactgaacc aactcatgag tggtcagtgt cttttcgtag aatgcctacc gtactgtcat 2760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 2820

2761 tctcttaata cgtcacgacg gtattggtac tcactattgt gacgccggtt gaatgaagac 2820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 2880

2821 tgttgctagc ctcctggctt cctcgattgg cgaaaaaacg tgttgtaccc cctagtacat 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 2940

2881 tgagcggaac tagcaaccct tggcctcgac ttacttcggt atggtttgct gctcgcactg 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 3000

2941 tggtgctacg gacatcgtta ccgttgttgc aacgcgtttg ataattgacc gcttgatgaa 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 3060

3001 tgagatcgaa gggccgttgt taattatctg acctacctcc gcctatttca acgtcctggt 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 3120

3061 gaagacgcga gccgggaagg ccgaccgacc aaataacgac tatttagacc tcggccactc 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 3180

3121 gcacccagag cgccatagta acgtcgtgac cccggtctac cattcgggag ggcatagcat 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 3240

3181 caatagatgt gctgcccctc agtccgttga tacctacttg ctttatctgt ctagcgactc 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 3300

3241 tatccacgga gtgactaatt cgtaaccatt gacagtctgg ttcaaatgag tatatatgaa 3300

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 3360

3301 atctaactaa attttgaagt aaaaattaaa ttttcctaga tccacttcta ggaaaaacta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 3420

3361 ttagagtact ggttttaggg aattgcactc aaaagcaagg tgactcgcag tctggggcat 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 3480

3421 cttttctagt ttcctagaag aactctagga aaaaaagacg cgcattagac gacgaacgtt 3480

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 3540

3481 tgtttttttg gtggcgatgg tcgccaccaa acaaacggcc tagttctcga tggttgagaa 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 3600

3541 aaaggcttcc attgaccgaa gtcgtctcgc gtctatggtt tatgacaaga agatcacatc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 3660

3601 ggcatcaatc cggtggtgaa gttcttgaga catcgtggcg gatgtatgga gcgagacgat 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 3720

3661 taggacaatg gtcaccgacg acggtcaccg ctattcagca cagaatggcc caacctgagt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 3780

3721 tctgctatca atggcctatt ccgcgtcgcc agcccgactt gccccccaag cacgtgtgtc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 3840

3781 gggtcgaacc tcgcttgctg gatgtggctt gactctatgg atgtcgcact cgatactctt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 3900

3841 tcgcggtgcg aagggcttcc ctctttccgc ctgtccatag gccattcgcc gtcccagcct 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 3960

3901 tgtcctctcg cgtgctccct cgaaggtccc cctttgcgga ccatagaaat atcaggacag 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 4020

3961 cccaaagcgg tggagactga actcgcagct aaaaacacta cgagcagtcc ccccgcctcg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 4080

4021 gatacctttt tgcggtcgtt gcgccggaaa aatgccaagg accggaaaac gaccggaaaa 4080

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gctcacatgg ctcgac.... .......... .......... .......... .......... 4096

4081 cgagtgtacc gagctg.... .......... .......... .......... .......... 4096

.......... .......... .......... .......... .......... ..........