Sequence of pAG193

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Sequence of pAG193_CORO1C

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Sequence of pAG193 CORO1C 3'UTR : 4109 BP; 1154 A; 863 C; 956 G; 1136 T; 0 other;

BglII(1) .......... .......... .......... .......... ..........

1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 60

1 tctagacgcg tcgtggtacc ggactttatt ggagactttc tccttgaacc aatccatgga 60

.......... .......... .......... .......... .......... ..........

.......... PvuII(80) .......... .......... .......... ..........

61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 120

61 agactccgcc tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc 120

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 180

121 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 180

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 240

181 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 240

.......... ...... SV40_enh SV40_pro ...... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 300

241 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 300

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 360

301 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc 360

........ SV40_pro SV40_pro SV40_origin SV40pro_F_pri ........

.......... .......... .......... .......... .......... ..........

361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 420

361 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt 420

SV40_pro .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... PstI(462) ..........

421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 480

421 tcgaactaag aagactgtgt tgtcagagct tgaattcgac gtcttcaacc agcactccgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 540

481 gacccgtcca ttcatagttc caatgttctg tccaaattcc tctggttatc tttgacccga 540

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 600

541 acagctctgt ctcttctgag aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg 600

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 660

601 tgaaacggaa agagaggtgt ccacaggtga gggtcaagtt aatgtcgaga attccgatct 660

.......... chim_int .......... .......... .......... ..........

.......... .......... NheI(684) BstBI(700) .......... ..........

661 gtacttaata cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa 720

661 catgaattat gctgagtgat atccgatcgg tggtactgaa gctttcaaat actaggtctt 720

.......... T7_pro .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

721 caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt 780

721 gtttcctttg cctactattg accaggcgtc accacccggt ctacatttgt ttacttacaa 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt 840

781 gaactaagta aataattaat aatactaagt ctttttgtac gtcttttacg acaataaaaa 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca 900

841 aatgtaccat tgcgccggag aagaataaat accgctgtac aacacggtgt ataactcggt 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat 960

901 catcgcgcca cataatatgg tctggaataa ccatacccgt ttagtccgtt tagaccatta 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat 1020

961 ccaagaatat ccaatgaact agtaatgttt atagaatgac gtaccaaact tgaagaatta 1020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat 1080

1021 aatggtttct tctagtaaaa acagccggta ctaaccccac gaacaaaccg taaagtaata 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg 1140

1081 tcgatactcg tagttctatt ctagtttcgt tatcaagtgc gactttcaca tcatctacac 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa 1200

1141 taacttagta ccctacttac cggactataa cttcttctat aacgcaacta gtttagactt 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa 1260

1201 cttcctcttt tttaccaaaa cctcttattg aagaagcacc tttggtacaa cggtagtttt 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt caaagagaaa 1320

1261 tagtactctt tcaatcttgg tcttcttaaa cgtcgtatag aacttggtaa gtttctcttt 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt 1380

1321 ccacttcaag cagcaggttg taatagtacc ggagcacttt agggcaatca ttttccacca 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc aagtgatgat 1440

1381 tttggactgc aacatgttta acaatcctta atattacgaa tagatgcacg ttcactacta 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat tgttgaaggt 1500

1441 aatggttttt acaaataact tagcctgggt cctaagaaaa ggttacgata acaacttcca 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa 1560

1501 cggttcttca aaggattatg acttaaacag tttcattttc cagaagtaaa aagcgttctt 1560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat 1620

1561 ctacgtggac tactttaccc ttttatatag tttagcaagc aactcgctca agagttttta 1620

.......... .......... ORF 1 .......... .......... ..........

.... XhoI(1639),SacI(1640) .... .......... .......... ..........

1621 gaacaataat tctaggagct cgagctggtt attggtgtgg tcctagggag ggcggaaagg 1680

1621 cttgttatta agatcctcga gctcgaccaa taaccacacc aggatccctc ccgcctttcc 1680

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gaggcactgc catttggaga cattccattt cagatttgtc aaccagcgat aggccacatt 1740

1681 ctccgtgacg gtaaacctct gtaaggtaaa gtctaaacag ttggtcgcta tccggtgtaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccagtaagaa ctcaatttgt ctcccaaatt tgcagaaaca aaacgtgatt taaaagctga 1800

1741 ggtcattctt gagttaaaca gagggtttaa acgtctttgt tttgcactaa attttcgact 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gctttttatc agaaagcttt tttgatgttt taagtgttat gtgacttgtt gaacttttta 1860

1801 cgaaaaatag tctttcgaaa aaactacaaa attcacaata cactgaacaa cttgaaaaat 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aaaagtgcta cttttaaaat cccagatact ctgaatttta gaaaacaaac taattctgat 1920

1861 ttttcacgat gaaaatttta gggtctatga gacttaaaat cttttgtttg attaagacta 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgtgtcgtgc ccaagtaccc ttttttttta atgaataggg accaatgcca cattgctttt 1980

1921 acacagcacg ggttcatggg aaaaaaaaat tacttatccc tggttacggt gtaacgaaaa 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... SpeI(2027) ..........

1981 tatatttctt tcttttttaa tgttgccaaa accaaaagta gctttgacta gtgttctaga 2040

1981 atataaagaa agaaaaaatt acaacggttt tggttttcat cgaaactgat cacaagatct 2040

.......... .......... .......... .......... .......... ..........

NotI(2042),EagI(2042) ........ .......... .......... ..........

2041 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag 2100

2041 cgccggcgaa gctcgtctgt actattctat gtaactactc aaacctgttt ggtgttgatc 2100

.......... .......... ........ SV40_PA_term EBV_rev_pri ........

.......... .......... .......... .......... .......... ..........

2101 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 2160

2101 ttacgtcact ttttttacga aataaacact ttaaacacta cgataacgaa ataaacattg 2160

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 2220

2161 gtaatattcg acgttatttg ttcaattgtt gttgttaacg taagtaaaat acaaagtcca 2220

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat 2280

2221 agtccccctc cacaccctcc aaaaaatttc gttcattttg gagatgttta caccatttta 2280

.......... .......... .......... .......... .......... ..........

BamHI(2287) ........ .......... .......... .......... ..........

2281 cgataaggat ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 2340

2281 gctattccta ggtccaccgt gaaaagcccc tttacacgcg ccttggggat aaacaaataa 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 2400

2341 aaagatttat gtaagtttat acataggcga gtactctgtt attgggacta tttacgaagt 2400

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 2460

2401 tattataact ttttccttct catactcata agttgtaaag gcacagcggg aataagggaa 2460

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 2520

2461 aaaacgccgt aaaacggaag gacaaaaacg agtgggtctt tgcgaccact ttcattttct 2520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 2580

2521 acgacttcta gtcaacccac gtgctcaccc aatgtagctt gacctagagt tgtcgccatt 2580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 2640

2581 ctaggaactc tcaaaagcgg ggcttcttgc aaaaggttac tactcgtgaa aatttcaaga 2640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 2700

2641 cgatacaccg cgccataata gggcataact gcggcccgtt ctcgttgagc cagcggcgta 2700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 2760

2701 tgtgataaga gtcttactga accaactcat gagtggtcag tgtcttttcg tagaatgcct 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 2820

2761 accgtactgt cattctctta atacgtcacg acggtattgg tactcactat tgtgacgccg 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 2880

2821 gttgaatgaa gactgttgct agcctcctgg cttcctcgat tggcgaaaaa acgtgttgta 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 2940

2881 ccccctagta cattgagcgg aactagcaac ccttggcctc gacttacttc ggtatggttt 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 3000

2941 gctgctcgca ctgtggtgct acggacatcg ttaccgttgt tgcaacgcgt ttgataattg 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 3060

3001 accgcttgat gaatgagatc gaagggccgt tgttaattat ctgacctacc tccgcctatt 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 3120

3061 tcaacgtcct ggtgaagacg cgagccggga aggccgaccg accaaataac gactatttag 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 3180

3121 acctcggcca ctcgcaccca gagcgccata gtaacgtcgt gaccccggtc taccattcgg 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 3240

3181 gagggcatag catcaataga tgtgctgccc ctcagtccgt tgatacctac ttgctttatc 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 3300

3241 tgtctagcga ctctatccac ggagtgacta attcgtaacc attgacagtc tggttcaaat 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 3360

3301 gagtatatat gaaatctaac taaattttga agtaaaaatt aaattttcct agatccactt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 3420

3361 ctaggaaaaa ctattagagt actggtttta gggaattgca ctcaaaagca aggtgactcg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 3480

3421 cagtctgggg catcttttct agtttcctag aagaactcta ggaaaaaaag acgcgcatta 3480

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3481 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 3540

3481 gacgacgaac gtttgttttt ttggtggcga tggtcgccac caaacaaacg gcctagttct 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 3600

3541 cgatggttga gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg gtttatgaca 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 3660

3601 agaagatcac atcggcatca atccggtggt gaagttcttg agacatcgtg gcggatgtat 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 3720

3661 ggagcgagac gattaggaca atggtcaccg acgacggtca ccgctattca gcacagaatg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 3780

3721 gcccaacctg agttctgcta tcaatggcct attccgcgtc gccagcccga cttgcccccc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 3840

3781 aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg cttgactcta tggatgtcgc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 3900

3841 actcgatact ctttcgcggt gcgaagggct tccctctttc cgcctgtcca taggccattc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 3960

3901 gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc ggaccataga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 4020

3961 aatatcagga cagcccaaag cggtggagac tgaactcgca gctaaaaaca ctacgagcag 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 4080

4021 tccccccgcc tcggatacct ttttgcggtc gttgcgccgg aaaaatgcca aggaccggaa 4080

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttgctggcct tttgctcaca tggctcgac. .......... .......... .......... 4109

4081 aacgaccgga aaacgagtgt accgagctg. .......... .......... .......... 4109

.......... .......... .......... .......... .......... ..........