Sequence of pAG303GAL-ccdB

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Sequence of pAG303GAL-ccdB

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Sequence of pAG303GAL-ccdB : 6843 BP; 1845 A; 1540 C; 1626 G; 1832 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

1 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 60

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

61 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

121 aaccgcccac agccccgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga 240

181 tggtattaag gcaaaattct cgaaccactc gcgatcctca gtgacggtcc atagcaaact 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt 300

241 tgtgccgtaa tcagtccctt cagtattgtg tcaggaaagg gcgttaaaag aaaaagataa 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt 360

301 tgagaaccgg aggagatcat gtgagatata aaaaaatacg gagccattac taaaagtaaa 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa 420

361 aaaaaaaagg tggatcgcct actgagaaaa aaaaagaatc gctaaccgta atagtgtatt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca 480

421 acttaatatg taatatattt cattacacta aagaagcttc ttatatgatt ttttactcgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa 540

481 ccgttctatt tgcttccgtt tctactgtct cgtctttcgg gatcatttcg cataatgttt 540

.......... .......... .......... .......... HIS3 ORF2 ..........

.......... .......... .......... .......... .......... ..........

541 tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc 600

541 actttggttc taagtctaac gctagagaaa tttcccacca ggggatcgct atctcgtgag 600

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat 660

601 ctagaagggt ctttttctcc gtcttcgtca tcgtcttgtc cggtgtgtta gcgttcacta 660

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc 720

661 attgcaggtg tgtccatatc ccaaagacct ggtatactat gtacgagacc ggttcgtaag 720

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac 780

721 gccgaccagc gattagcaac tcacgtaacc actgaatgtg tatctgctgg tagtgtggtg 780

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt 840

781 acttctgacg ccctaacgag agccagttcg aaaatttctc cgggatgacc gcgcacctca 840

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga 900

841 tttttccaaa cctagtccta aacgcggaaa cctactccgt gaaaggtctc gccaccatct 900

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga 960

901 agaaagcttg tccggcatgc gtcaacagct tgaaccaaac gtttccctct ttcatcctct 960

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... NheI(1007) ..........

961 tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac 1020

961 agagagaacg ctctactagg gcgtaaaaga actttcgaaa cgtctccgat cgtcttaatg 1020

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... BclI(1051) .......... ..........

1021 cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa 1080

1021 ggaggtgcaa ctaacagacg ctccgttctt actagtagtg gcatcactct cacgcaagtt 1080

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc 1140

1081 ccgagaacgc caacggtatt ctcttcggtg gagcgggtta ccatggttgc tacaagggag 1140

.......... .......... HIS3 ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata 1200

1141 gtggtttcca caagaataca tcactgtggc taataaattt cgacgtcgta tgctatatat 1200

HIS3 ORF2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata 1260

1201 atgtacacat atatacatat ggatacttac agtcattcat acatatgctt gtcatactat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc 1320

1261 gacttctact gttccattac gtagtaagat atgcacagta agacttgctc cgcgcgaaag 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt 1380

1321 gaaaaaagaa aaacgaaaaa gaaaaaaaaa gagaacttga gctgcctagt atacgccaca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1440

1381 ctttatggcg tgtctacgca ttcctctttt atggcgtagt cctttaacat ttgcaattat 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1500

1441 aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag taaaaaattg gttatccggc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1560

1501 tttagccgtt ttagggaata tttagttttc ttatctggct ctatcccaac tcacaacaag 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1620

1561 gtcaaacctt gttctcaggt gataatttct tgcacctgag gttgcagttt cccgcttttt 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1680

1621 ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg gattagttca aaaaacccca 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1740

1681 gctccacggc atttcgtgat ttagccttgg gatttccctc gggggctaaa tctcgaactg 1740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1800

1741 cccctttcgg ccgcttgcac cgctctttcc ttcccttctt tcgctttcct cgcccgcgat 1800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1860

1801 cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg gtgtgggcgg cgcgaattac 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1920

1861 gcggcgatgt cccgcgcagc gcggtaagcg gtaagtccga cgcgttgaca acccttcccg 1920

.......... .......... .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc 1980

1921 ctagccacgc ccggagaagc gataatgcgg tcgaccgctt cccccctaca cgacgttccg 1980

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 2040

1981 ctaattcaac ccattgcggt cccaaaaggg tcagtgctgc aacattttgc tgccggtcac 2040

.......... .......... lacZ_a M13_pUC_f .......... M13_f20

.......... .......... .......... SacI(2077) .......... ..........

2041 aattgtaata cgactcacta tagggcgaat tggagctcta gtacggatta gaagccgccg 2100

2041 ttaacattat gctgagtgat atcccgctta acctcgagat catgcctaat cttcggcggc 2100

.......... T7_pro .......... .......... .......... GAL1_pro

.......... .......... .......... .......... .......... AgeI(2153)

2101 agcgggcgac agccctccga cggaagactc tcctccgtgc gtcctcgtct tcaccggtcg 2160

2101 tcgcccgctg tcgggaggct gccttctgag aggaggcacg caggagcaga agtggccagc 2160

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgttcctgaa acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa gattctacaa 2220

2161 gcaaggactt tgcgtctaca cggagcgcgg cgtgacgagg cttgttattt ctaagatgtt 2220

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tactagcttt tatggttatg aagaggaaaa attggcagta acctggcccc acaaaccttc 2280

2221 atgatcgaaa ataccaatac ttctcctttt taaccgtcat tggaccgggg tgtttggaag 2280

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaattaacga atcaaattaa caaccatagg atgataatgc gattagtttt ttagccttat 2340

2281 tttaattgct tagtttaatt gttggtatcc tactattacg ctaatcaaaa aatcggaata 2340

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttctggggta attaatcagc gaagcgatga tttttgatct attaacagat atataaatgg 2400

2341 aagaccccat taattagtcg cttcgctact aaaaactaga taattgtcta tatatttacc 2400

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aaaagctgca taaccacttt aactaatact ttcaacattt tcagtttgta ttacttctta 2460

2401 ttttcgacgt attggtgaaa ttgattatga aagttgtaaa agtcaaacat aatgaagaat 2460

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttcaaatgtc ataaaagtat caacaaaaaa ttgttaatat acctctatac t ttaacgtca 2520

2461 aagtttacag tattttcata gttgtttttt aacaattata tggagatatg aaattgcagt 2520

.......... .......... GAL1_pro .......... GAL1_pri ..........

.......... XbaI(2540) SpeI(2546) .......... .......... ..........

2521 aggagaaaaa accccggatt ctagaactag tggatccccc atcacaagtt tgtacaaaaa 2580

2521 tcctcttttt tggggcctaa gatcttgatc acctaggggg tagtgttcaa acatgttttt 2580

GAL1_pro ......... pBluesSK_pri ......... .......... attR1

.......... .......... .......... .......... .......... ..........

2581 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa 2640

2581 tcgacttgct ctttgcattt tactatattt atagttatat aatttaatct aaaacgtatt 2640

.......... .......... attR1 attR2 .......... ..........

.......... .......... .......... .......... NotI(2690) ..........

2641 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta 2700

2641 ttttgtctga tgtattatga cattttgtgt tgtataggtc agtgataccg ccggcgtaat 2700

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag 2760

2701 ccgtggggtc cgaaatgtga aatacgaagg ccgagcatat tacacaccta aaactcaatc 2760

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat 2820

2761 ctaggcagct ctaaaagtcc tcgattcctt cgattttacc tcttttttta gtgacctata 2820

.......... .......... .......... ........ ORF1 CAT/CamR ........

.......... .......... .......... .......... .......... ..........

2821 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt 2880

2821 tggtggcaac tatatagggt taccgtagca tttcttgtaa aactccgtaa agtcagtcaa 2880

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta 2940

2881 cgagttacat ggatattggt ctggcaagtc gacctataat gccggaaaaa tttctggcat 2940

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat 3000

2941 ttctttttat tcgtgttcaa aataggccgg aaataagtgt aagaacgggc ggactactta 3000

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt 3060

3001 cgagtaggcc ttaaggcata ccgttacttt ctgccactcg accactatac cctatcacaa 3060

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa 3120

3061 gtgggaacaa tgtggcaaaa ggtactcgtt tgactttgca aaagtagcga gacctcactt 3120

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt 3180

3121 atggtgctgc taaaggccgt caaagatgtg tatataagcg ttctacaccg cacaatgcca 3180

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat 3240

3181 cttttggacc ggataaaggg atttcccaaa taactcttat acaaaaagca gagtcggtta 3240

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc 3300

3241 gggacccact caaagtggtc aaaactaaat ttgcaccggt tatacctgtt gaagaagcgg 3300

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... NcoI(3311) .......... .......... .......... ..........

3301 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg 3360

3301 gggcaaaagt ggtacccgtt tataatatgc gttccgctgt tccacgacta cggcgaccgc 3360

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta 3420

3361 taagtccaag tagtacggca gacactaccg aaggtacagc cgtcttacga attacttaat 3420

.......... ........ ORF1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc cgcgtggatc cggcttacta 3480

3421 gttgtcatga cgctactcac cgtcccgccc cgcatttgcg gcgcacctag gccgaatgat 3480

........ ORF1 CAT/CamR ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact 3540

3481 tttcggtcta ttgtcatacg cataaacgcg cgactaaaaa cgccatattc ttatatatga 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga 3600

3541 ctatacatat gggcttcata cagtttttct ccatacgata cttcgtcgca taatgtcact 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct 3660

3601 gtcaactgtc gctgtcgata gtcaacgagt tccgtatata ctacagttat agaggccaga 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga 3720

3661 ccattcgtgt tggtacgtct tacttcgggc agcagacgca cggcttgcga cctttcgcct 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga 3780

3721 tttagtcctt ccctaccgac tccagcgggc caaataactt tacttgccga gaaaacgact 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt 3840

3781 gctcttgtcc ccgaccactt tacgtcaaat tccaaatgtg gatattttct ctctcggcaa 3840

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... .......... SmaI(3884) ..........

3841 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga 3900

3841 tagcagacaa acacctacat gtctcactat aataactgtg cgggcccgct gcctaccact 3900

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 3960

3901 agggggaccg gtcacgtgca gacgacagtc tatttcagag ggcacttgaa atgggccacc 3960

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 4020

3961 acgtatagcc cctactttcg accgcgtact actggtggct ataccggtca cacggccaga 4020

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 4080

4021 ggcaatagcc ccttcttcac cgactagagt cggtggcgct tttactgtag tttttgcggt 4080

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag 4140

4081 aattggacta caagacccct tatatttaca gtccgaggga atatgtgtcg gtcagacgtc 4140

.......... ccdB .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc 4200

4141 cagctggtat cactgaccta tacaacacaa aatgtcataa tacatcagac aaaaaatacg 4200

.......... .......... ......... attR2 attR1 ......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg 4260

4201 ttttagatta aattatataa ctataaatat agtaaaatgc aaagagcaag tcgaaagaac 4260

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... ......... EcoRV(4292) ClaI(4303) XhoI(4317)

4261 tacaaagtgg tgatgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg 4320

4261 atgtttcacc actacccgac gtccttaagc tatagttcga atagctatgg cagctggagc 4320

.......... .......... .......... .......... ......... pBluesKS_pri

.......... .......... .......... .......... .......... ..........

4321 agtcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac 4380

4321 tcagtacatt aatcaataca gtgcgaatgt aagtgcggga ggggggtgta ggcgagattg 4380

.......... ... CYC1_pri pYESTrp_rev_pri CYC1_term ... ..........

.......... .......... .......... .......... .......... ..........

4381 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat 4440

4381 gcttttcctt cctcaatctg ttggacttca gatccaggga taaataaaaa aatatcaata 4440

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt 4500

4441 caatcataat tcttgcaata aatataaagt ttaaaaagaa aaaaaagaca tgtctgcgca 4500

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc 4560

4501 catgcgtaca ttgtaatatg acttttggaa cgaactcttc caaaaccctg cgagcttccg 4560

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tttaatttgc ggccggtacc cagcttttgt tccctttagt gagggttaat tccgagcttg 4620

4561 aaattaaacg ccggccatgg gtcgaaaaca agggaaatca ctcccaatta aggctcgaac 4620

CYC1_term .......... .......... .......... T3_pro ..........

.......... .......... .......... .......... .......... ..........

4621 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 4680

4621 cgcattagta ccagtatcga caaaggacac actttaacaa taggcgagtg ttaaggtgtg 4680

.......... M13_r .......... M13_pUC_r .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc 4740

4681 ttgtatcctc ggccttcgta tttcacattt cggaccccac ggattactca ctccattgag 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 4800

4741 tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc ctttggacag cacggtcgac 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 4860

4801 gtaattactt agccggttgc gcgcccctct ccgccaaacg cataacccgc gagaaggcga 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 4920

4861 aggagcgagt gactgagcga cgcgagccag caagccgacg ccgctcgcca tagtcgagtg 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 4980

4921 agtttccgcc attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 5040

4981 cgttttccgg tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta 5040

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5041 aggctcggcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 5100

5041 tccgagccgg ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccgacaggac tataaagata ccaggcgttc ccccctggaa gctccctcgt gcgctctcct 5160

5101 ggctgtcctg atatttctat ggtccgcaag gggggacctt cgagggagca cgcgagagga 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 5220

5161 caaggctggg acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 5280

5221 gaaagagtta cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 5340

5281 ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 5400

5341 gaactcaggt tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 5460

5401 taatcgtctc gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 5520

5461 ccgatgtgat cttcctgtca taaaccatag acgcgagacg acttcggtca atggaagcct 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 5580

5521 ttttctcaac catcgagaac taggccgttt gtttggtggc gaccatcgcc accaaaaaaa 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 5640

5581 caaacgttcg tcgtctaatg cgcgtctttt tttcctagag ttcttctagg aaactagaaa 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 5700

5641 agatgcccca gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa ccagtactct 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 5760

5701 aatagttttt cctagaagtg gatctaggaa aatttaattt ttacttcaaa atttagttag 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 5820

5761 atttcatata tactcatttg aaccagactg tcaatggtta cgaattagtc actccgtgga 5820

.......... .......... .......... ....... ORF3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

5821 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactgcccgt cgtgtagata 5880

5821 tagagtcgct agacagataa agcaagtagg tatcaacgga ctgacgggca gcacatctat 5880

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 5940

5881 tgatgctatg ccctcccgaa tggtagaccg gggtcacgac gttactatgg cgctctgggt 5940

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 6000

5941 gcgagtggcc gaggtctaaa tagtcgttat ttggtcggtc ggccttcccg gctcgcgtct 6000

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 6060

6001 tcaccaggac gttgaaatag gcggaggtag gtcagataat taacaacggc ccttcgatct 6060

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 6120

6061 cattcatcaa gcggtcaatt atcaaacgcg ttgcaacaac ggtaacgatg tccgtagcac 6120

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 6180

6121 cacagtgcga gcagcaaacc ataccgaagt aagtcgaggc caagggttgc tagttccgct 6180

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gttacatgat cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt 6240

6181 caatgtacta gggggtacaa cacttttttt cgccaatcga ggaagccagg aggctagcaa 6240

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 6300

6241 cagtcttcat tcaaccggcg tcacaatagt gagtaccaat accgtcgtga cgtattaaga 6300

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 6360

6301 gaatgacagt acggtaggca ttctacgaaa agacactgac cactcatgag ttggttcagt 6360

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 6420

6361 aagactctta tcacatacgc cgctggctca acgagaacgg gccgcagtta tgccctatta 6420

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 6480

6421 tggcgcggtg tatcgtcttg aaattttcac gagtagtaac cttttgcaag aagccccgct 6480

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 6540

6481 tttgagagtt cctagaatgg cgacaactct aggtcaagct acattgggtg agcacgtggg 6540

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 6600

6541 ttgactagaa gtcgtagaaa atgaaagtgg tcgcaaagac ccactcgttt ttgtccttcc 6600

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 6660

6601 gttttacggc gttttttccc ttattcccgc tgtgccttta caacttatga gtatgagaag 6660

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 6720

6661 gaaaaagtta taataacttc gtaaatagtc ccaataacag agtactcgcc tatgtataaa 6720

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

6721 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 6780

6721 cttacataaa tctttttatt tgtttatccc caaggcgcgt gtaaaggggc ttttcacggt 6780

.......... .......... .......... .......... .......... ..........

AatII(6788) ........ .......... .......... .......... ..........

6781 cctgacgtct tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 6840

6781 ggactgcaga ataatagtac tgtaattgga tatttttatc cgcatagtgc tccgggaaag 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gtc....... .......... .......... .......... .......... .......... 6843

6841 cag....... .......... .......... .......... .......... .......... 6843

.......... .......... .......... .......... .......... ..........