U0U03LOCUS pAG414GAL-ccdB 7188 bp DNA SYN 25-Aug-2007 DEFINITION pAG414GAL-ccdB ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7188 /organism="pAG414GAL-ccdB" /mol_type="other DNA" misc_feature complement(65..583) /label="CEN6_ARS4" promoter 645..673 /label="AmpR_promoter" CDS 715..1575 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 715..1575 /label="Ampicillin" /gene="Ampicillin" rep_origin 1730..2349 /label="pBR322_origin" misc_feature 2701..2723 /label="M13_pUC_rev_primer" misc_feature 2722..2740 /label="M13_reverse_primer" promoter 2757..2776 /label="T3_promoter" promoter 2798..3248 /label="GAL1_promoter" misc_feature 3211..3234 /label="GAL1_primer" misc_feature 3255..3271 /label="pBluescriptSK_primer" misc_feature 3279..3403 /label="attR1" misc_feature complement(3303..3403) /label="attR2" promoter 3428..3451 /label="lac_promoter" CDS 3512..4171 /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 2" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene 3512..4171 /label="CAT/CamR" /gene="CAT/CamR" misc_feature 4516..4821 /label="ccdB" misc_feature 4862..4962 /label="attR2" misc_feature complement(4862..4969) /label="attR1" misc_feature complement(5020..5036) /label="pBluescriptKS_primer" misc_feature complement(5054..5072) /label="CYC1_primer" misc_feature complement(5054..5072) /label="pYESTrp_rev_primer" terminator 5045..5284 /label="CYC1_terminator" promoter complement(5304..5322) /label="T7_promoter" misc_feature complement(5332..5348) /label="M13_forward20_primer" misc_feature complement(5341..5363) /label="M13_pUC_fwd_primer" misc_feature 5341..5484 /label="lacZ_a" rep_origin 5510..5816 /label="f1_origin" CDS complement(6047..6721) /label="TRP1" /gene="TRP1" /note="ORF frame 2" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" gene complement(6047..6721) /label="TRP1" /gene="TRP1" misc_feature 7138..7160 /label="pGEX_3_primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata 121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt 181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa 241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata 301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct 361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata 421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact 481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa 541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg 601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact 2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg 2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 2761 aaccctcact aaagggaaca aaagctggag ctctagtacg gattagaagc cgccgagcgg 2821 gcgacagccc tccgacggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc 2881 ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta 2941 gcttttatgg ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatt 3001 aacgaatcaa attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg 3061 gggtaattaa tcagcgaagc gatgattttt gatctattaa cagatatata aatggaaaag 3121 ctgcataacc actttaacta atactttcaa cattttcagt ttgtattact tcttattcaa 3181 atgtcataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag 3241 aaaaaacccc ggattctaga actagtggat cccccatcac aagtttgtac aaaaaagctg 3301 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac 3361 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac 3421 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc 3481 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac 3541 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca 3601 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa 3661 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca 3721 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc 3781 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca 3841 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa 3901 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg 3961 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt 4021 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 4081 ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 4141 gtactgcgat gagtggcagg gcggggcgta aacgccgcgt ggatccggct tactaaaagc 4201 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat 4261 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt 4321 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa 4381 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc 4441 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga 4501 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 4561 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc 4621 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 4681 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 4741 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 4801 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga 4861 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat 4921 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa 4981 agtggtgatg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagtca 5041 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa 5101 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag 5161 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg 5221 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa 5281 tttgcggccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 5341 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 5401 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 5461 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc 5521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 5581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 5641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 5701 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 5761 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 5821 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 5881 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 5941 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 6001 ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat 6061 ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc 6121 ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca 6181 gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca acccagtcag 6241 aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca aacaagggaa 6301 taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct tttggaaata 6361 cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac gactcatctc 6421 catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca tcctccttag 6481 gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt ttatatgctt 6541 ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt ctattgggca 6601 cacatataat acccagcaag tcagcatcgg aatctagagc acattctgcg gcctctgtgc 6661 tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta ataacagaca 6721 tactccaagc tgcctttgtg tgcttaatca cgtatactca cgtgctcaat agtcaccaat 6781 gccctccctc ttggccctct ccttttcttt tttcgaccga attaattctt aatcggcaaa 6841 aaaagaaaag ctccggatca agattgtacg taaggtgaca agctattttt caataaagaa 6901 tatcttccac tactgccatc tggcgtcata actgcaaagt acacatatat tacgatgctg 6961 tctattaaat gcttcctata ttatatatat agtaatgtcg tttatggtgc actctcagta 7021 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 7081 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 7141 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga //pAG414GAL-ccdB "Times New Roman*U0CAG CEN6_ARS4"Times New RomanU0CAmpR_pro"Times New RomanU0CɆ'ORF1"Times New Roman(U0' Ampicillin"Times New RomanU0C-  pBR322_origin"Times New RomanU0C  M13_pUC_rev"Times New RomanU0CɆ M13_rev"Times New RomanU0 T3_pro"Times New RomanU0C GAL1_pro"Times New RomanU0C  GAL1_primer"Times New RomanU0CɆ  pBluesSK_pri"Times New RomanU0 K attR1"Times New RomanU0C K attR2"Times New RomanU0Cd {  lac_promoter"Times New RomanU0CɆ K Orf frame 2"Times New RomanU0 KCAT/CamR"Times New RomanU0CccdB"Times New RomanU0CbattR2"Times New RomanU0CɆiattR1"Times New Roman(U0 pBluesKS_pri"Times New RomanU0C CYC1_primer"Times New RomanU0C pYESTrp_rev"Times New RomanU0CɆCYC1_terminator"Times New Roman*U0T7"Times New RomanU0CM13_f20"Times New RomanU0C M13_pUC_f"Times New RomanU0CɆllacZ_a"Times New Roman)U0 f1_origin"Times New RomanU0CAORF2"Times New RomanU0CATRP1"Times New RomanU0CɆ pGEX_3_primer"Times New Roman U0AgeI(2868), SacI(2792)"Times New Roman4 =IUU< U0SpeI"Times New Roman $7JJY U0NotI"Times New RomanM +?QQ` U0NcoI"Times New Roman ZR1JC*CjR U0SmaI"Times New Roman  U0ClaI(5018), XhoI(5032)"Times New Roman5'e U0KpnI"Times New RomanVXYYR` U0test"Times New Roman {~ssg U0test"Times New Roman. )  Xwg U0test"Times New Roman VTJ4#`; U0test"Times New RomancUA6c6