Sequence of pAG414GAL

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Sequence of pAG414GAL

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Sequence of pAG414GAL-ccdB : 7188 BP; 1971 A; 1645 C; 1570 G; 2002 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 60

1 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata 120

61 gaatcctgcc tagcgaacgg acattgaatg tgcgcggagc atagaaaatt actaccttat 120

.......... .......... .......... CEN6_ARS4 .......... ..........

.......... .......... .......... .......... .......... ..........

121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt 180

121 taaaccctta aatgagacac aaataaataa aaatacaaaa cataaaccta aaatctttca 180

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa 240

181 tttatttctt ccatcttctc aatgccttac ttcttttttt ttatttgttt ccaaattttt 240

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata 300

241 taaagttgtt tttcgcatga aatgtatata taaataatct gttcttttcg tctaatttat 300

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct 360

301 ctatatgtaa gctaattgct attcatttta cattttagtg tcctaaaagc acacaccaga 360

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata 420

361 agatgtgtct gttctacttt gttaagccgt aattatggac tctcgtcctt ctcgttctat 420

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact 480

421 tttccatcat aaacaaccgc tagggggatc tcagaaaatg tagaagcctt ttgtttttga 480

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa 540

481 taaaaaagaa attaaagaaa aaaatgaaag ataaaaatta aatatataaa tataattttt 540

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg 600

541 taaatttaat attaataaaa atatcgtgca ctacttttcc tgggtccacc gtgaaaagcc 600

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 660

601 cctttacacg cgccttgggg ataaacaaat aaaaagattt atgtaagttt atacataggc 660

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 720

661 gagtactctg ttattgggac tatttacgaa gttattataa ctttttcctt ctcatactca 720

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 780

721 taagttgtaa aggcacagcg ggaataaggg aaaaaacgcc gtaaaacgga aggacaaaaa 780

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 840

781 cgagtgggtc tttgcgacca ctttcatttt ctacgacttc tagtcaaccc acgtgctcac 840

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 900

841 ccaatgtagc ttgacctaga gttgtcgcca ttctaggaac tctcaaaagc ggggcttctt 900

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 960

901 gcaaaaggtt actactcgtg aaaatttcaa gacgatacac cgcgccataa tagggcataa 960

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1020

961 ctgcggcccg ttctcgttga gccagcggcg tatgtgataa gagtcttact gaaccaactc 1020

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1080

1021 atgagtggtc agtgtctttt cgtagaatgc ctaccgtact gtcattctct taatacgtca 1080

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1140

1081 cgacggtatt ggtactcact attgtgacgc cggttgaatg aagactgttg ctagcctcct 1140

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 1200

1141 ggcttcctcg attggcgaaa aaacgtgttg taccccctag tacattgagc ggaactagca 1200

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 1260

1201 acccttggcc tcgacttact tcggtatggt ttgctgctcg cactgtggtg ctacggacat 1260

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1320

1261 cgttaccgtt gttgcaacgc gtttgataat tgaccgcttg atgaatgaga tcgaagggcc 1320

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1380

1321 gttgttaatt atctgaccta cctccgccta tttcaacgtc ctggtgaaga cgcgagccgg 1380

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1440

1381 gaaggccgac cgaccaaata acgactattt agacctcggc cactcgcacc cagagcgcca 1440

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1500

1441 tagtaacgtc gtgaccccgg tctaccattc gggagggcat agcatcaata gatgtgctgc 1500

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1560

1501 ccctcagtcc gttgatacct acttgcttta tctgtctagc gactctatcc acggagtgac 1560

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1620

1561 taattcgtaa ccattgacag tctggttcaa atgagtatat atgaaatcta actaaatttt 1620

ORF1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1680

1621 gaagtaaaaa ttaaattttc ctagatccac ttctaggaaa aactattaga gtactggttt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1740

1681 tagggaattg cactcaaaag caaggtgact cgcagtctgg ggcatctttt ctagtttcct 1740

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1800

1741 agaagaactc taggaaaaaa agacgcgcat tagacgacga acgtttgttt ttttggtggc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1860

1801 gatggtcgcc accaaacaaa cggcctagtt ctcgatggtt gagaaaaagg cttccattga 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1920

1861 ccgaagtcgt ctcgcgtcta tggtttatga caggaagatc acatcggcat caatccggtg 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1980

1921 gtgaagttct tgagacatcg tggcggatgt atggagcgag acgattagga caatggtcac 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2040

1981 cgacgacggt caccgctatt cagcacagaa tggcccaacc tgagttctgc tatcaatggc 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2100

2041 ctattccgcg tcgccagccc gacttgcccc ccaagcacgt gtgtcgggtc gaacctcgct 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2160

2101 tgctggatgt ggcttgactc tatggatgtc gcactcgata ctctttcgcg gtgcgaaggg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2220

2161 cttccctctt tccgcctgtc cataggccat tcgccgtccc agccttgtcc tctcgcgtgc 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2280

2221 tccctcgaag gtcccccttt gcggaccata gaaatatcag gacagcccaa agcggtggag 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2340

2281 actgaactcg cagctaaaaa cactacgagc agtccccccg cctcggatac ctttttgcgg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2400

2341 tcgttgcgcc ggaaaaatgc caaggaccgg aaaacgaccg gaaaacgagt gtacaagaaa 2400

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2460

2401 ggacgcaata ggggactaag acacctattg gcataatggc ggaaactcac tcgactatgg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2520

2461 cgagcggcgt cggcttgctg gctcgcgtcg ctcagtcact cgctccttcg ccttctcgcg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2580

2521 ggttatgcgt ttggcggaga ggggcgcgca accggctaag taattacgtc gaccgtgctg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact 2640

2581 tccaaagggc tgacctttcg cccgtcactc gcgttgcgtt aattacactc aatggagtga 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg 2700

2641 gtaatccgtg gggtccgaaa tgtgaaatac gaaggccgag gatacaacac accttaacac 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 2760

2701 tcgcctattg ttaaagtgtg tcctttgtcg atactggtac taatgcggtt cgcgcgttaa 2760

......... M13_pUC_rev ......... M13_rev .......... ..........

.......... .......... .......... SacI(2792) .......... ..........

2761 aaccctcact aaagggaaca aaagctggag ctctagtacg gattagaagc cgccgagcgg 2820

2761 ttgggagtga tttcccttgt tttcgacctc gagatcatgc ctaatcttcg gcggctcgcc 2820

T3_pro .......... .......... .......... GAL1_pro ..........

.......... .......... .......... .......... .......... ..........

2821 gcgacagccc tccgacggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc 2880

2821 cgctgtcggg aggctgcctt ctgagaggag gcacgcagga gcagaagtgg ccagcgcaag 2880

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta 2940

2881 gactttgcgt ctacacggag cgcggcgtga cgaggcttgt tatttctaag atgttatgat 2940

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcttttatgg ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatt 3000

2941 cgaaaatacc aatacttctc ctttttaacc gtcattggac cggggtgttt ggaagtttaa 3000

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aacgaatcaa attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg 3060

3001 ttgcttagtt taattgttgg tatcctacta ttacgctaat caaaaaatcg gaataaagac 3060

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gggtaattaa tcagcgaagc gatgattttt gatctattaa cagatatata aatggaaaag 3120

3061 cccattaatt agtcgcttcg ctactaaaaa ctagataatt gtctatatat ttaccttttc 3120

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctgcataacc actttaacta atactttcaa cattttcagt ttgtattact tcttattcaa 3180

3121 gacgtattgg tgaaattgat tatgaaagtt gtaaaagtca aacataatga agaataagtt 3180

.......... .......... GAL1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atgtcataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa c gtcaaggag 3240

3181 tacagtattt tcatagttgt tttttaacaa ttatatggag atatgaaatt gcagttcctc 3240

.......... .......... GAL1_pro ......... GAL1_primer .........

.......... .......... SpeI(3261) .......... .......... ..........

3241 aaaaaacccc ggattctaga actagtggat cccccatcac aagtttgtac aaaaaagctg 3300

3241 ttttttgggg cctaagatct tgatcaccta gggggtagtg ttcaaacatg ttttttcgac 3300

.......... ......... pBluesSK_pri ......... attR1 ..........

.......... .......... .......... .......... .......... ..........

3301 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac 3360

3301 ttgctctttg cattttacta tatttatagt tatataattt aatctaaaac gtattttttg 3360

.......... .......... attR1 attR2 .......... ..........

.......... .......... .......... .......... NotI(3405) ..........

3361 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac 3420

3361 tctgatgtat tatgacattt tgtgttgtat aggtcagtga taccgccggc gtaatccgtg 3420

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc 3480

3421 gggtccgaaa tgtgaaatac gaaggccgag catattacac acctaaaact caatcctagg 3480

......... lac_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac 3540

3481 cagctctaaa agtcctcgat tccttcgatt ttacctcttt ttttagtgac ctatatggtg 3540

.......... .......... .......... ..... Orf frame 2 CAT/CamR .....

.......... .......... .......... .......... .......... ..........

3541 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca 3600

3541 gcaactatat agggttaccg tagcatttct tgtaaaactc cgtaaagtca gtcaacgagt 3600

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa 3660

3601 tacatggata ttggtctggc aagtcgacct ataatgccgg aaaaatttct ggcatttctt 3660

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca 3720

3661 tttattcgtg ttcaaaatag gccggaaata agtgtaagaa cgggcggact acttacgagt 3720

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc 3780

3721 aggccttaag gcataccgtt actttctgcc actcgaccac tataccctat cacaagtggg 3780

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca 3840

3781 aacaatgtgg caaaaggtac tcgtttgact ttgcaaaagt agcgagacct cacttatggt 3840

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa 3900

3841 gctgctaaag gccgtcaaag atgtgtatat aagcgttcta caccgcacaa tgccactttt 3900

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg 3960

3901 ggaccggata aagggatttc ccaaataact cttatacaaa aagcagagtc ggttagggac 3960

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt 4020

3961 ccactcaaag tggtcaaaac taaatttgca ccggttatac ctgttgaaga agcgggggca 4020

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

NcoI(4026) .......... .......... .......... .......... ..........

4021 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 4080

4021 aaagtggtac ccgtttataa tatgcgttcc gctgttccac gactacggcg accgctaagt 4080

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 4140

4081 ccaagtagta cggcagacac taccgaaggt acagccgtct tacgaattac ttaatgttgt 4140

.......... ..... Orf frame 2 CAT/CamR ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtactgcgat gagtggcagg gcggggcgta aacgccgcgt ggatccggct tactaaaagc 4200

4141 catgacgcta ctcaccgtcc cgccccgcat ttgcggcgca cctaggccga atgattttcg 4200

..... Orf frame 2 CAT/CamR ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat 4260

4201 gtctattgtc atacgcataa acgcgcgact aaaaacgcca tattcttata tatgactata 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt 4320

4261 catatgggct tcatacagtt tttctccata cgatacttcg tcgcataatg tcactgtcaa 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa 4380

4321 ctgtcgctgt cgatagtcaa cgagttccgt atatactaca gttatagagg ccagaccatt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc 4440

4381 cgtgttggta cgtcttactt cgggcagcag acgcacggct tgcgaccttt cgccttttag 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga 4500

4441 tccttcccta ccgactccag cgggccaaat aactttactt gccgagaaaa cgactgctct 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 4560

4501 tgtccccgac cactttacgt caaattccaa atgtggatat tttctctctc ggcaatagca 4560

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... SmaI(4599) .......... ..........

4561 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc 4620

4561 gacaaacacc tacatgtctc actataataa ctgtgcgggc ccgctgccta ccactagggg 4620

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 4680

4621 gaccggtcac gtgcagacga cagtctattt cagagggcac ttgaaatggg ccaccacgta 4680

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 4740

4681 tagcccctac tttcgaccgc gtactactgg tggctatacc ggtcacacgg ccagaggcaa 4740

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 4800

4741 tagccccttc ttcaccgact agagtcggtg gcgcttttac tgtagttttt gcggtaattg 4800

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga 4860

4801 gactacaaga ccccttatat ttacagtccg agggaatatg tgtcggtcag acgtccagct 4860

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat 4920

4861 ggtatcactg acctatacaa cacaaaatgt cataatacat cagacaaaaa atacgtttta 4920

ccdB .......... ......... attR2 attR1 ......... ..........

.......... .......... .......... .......... .......... ..........

4921 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa 4980

4921 gattaaatta tataactata aatatagtaa aatgcaaaga gcaagtcgaa agaacatgtt 4980

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... ClaI(5018) .......... XhoI(5032)

4981 agtggtgatg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagtca 5040

4981 tcaccactac ccgacgtcct taagctatag ttcgaatagc tatggcagct ggagctcagt 5040

.......... .......... .......... ........ pBluesKS_pri CYC1_primer

.......... .......... .......... .......... .......... ..........

5041 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa 5100

5041 acattaatca atacagtgcg aatgtaagtg cgggaggggg gtgtaggcga gattggcttt 5100

........ CYC1_primer pYESTrp_rev ..... CYC1_terminator .......

.......... .......... .......... .......... .......... ..........

5101 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag 5160

5101 tccttcctca atctgttgga cttcagatcc agggataaat aaaaaaatat caatacaatc 5160

.......... ....... CYC1_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg 5220

5161 ataattcttg caataaatat aaagtttaaa aagaaaaaaa agacatgtct gcgcacatgc 5220

.......... ....... CYC1_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa 5280

5221 gtacattgta atatgacttt tggaacgaac tcttccaaaa ccctgcgagc ttccgaaatt 5280

.......... ....... CYC1_terminator ....... .......... ..........

.......... KpnI(5294) .......... .......... .......... ..........

5281 tttgcggccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 5340

5281 aaacgccggc catgggttaa gcgggatatc actcagcata atgcgcgcga gtgaccggca 5340

.......... .......... .......... T7 ...... M13_f20 M13_pUC_f

.......... .......... .......... .......... .......... ..........

5341 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 5400

5341 gcaaaatgtt gcagcactga cccttttggg accgcaatgg gttgaattag cggaacgtcg 5400

.......... M13_pUC_f lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 5460

5401 tgtaggggga aagcggtcga ccgcattatc gcttctccgg gcgtggctag cgggaagggt 5460

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc 5520

5461 tgtcaacgcg tcggacttac cgcttaccgc gctgcgcggg acatcgccgc gtaattcgcg 5520

.......... lacZ_a .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

5521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 5580

5521 ccgcccacac caccaatgcg cgtcgcactg gcgatgtgaa cggtcgcggg atcgcgggcg 5580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 5640

5581 aggaaagcga aagaagggaa ggaaagagcg gtgcaagcgg ccgaaagggg cagttcgaga 5640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 5700

5641 tttagccccc gagggaaatc ccaaggctaa atcacgaaat gccgtggagc tggggttttt 5700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 5760

5701 tgaactaatc ccactaccaa gtgcatcacc cggtagcggg actatctgcc aaaaagcggg 5760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 5820

5761 aaactgcaac ctcaggtgca agaaattatc acctgagaac aaggtttgac cttgttgtga 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 5880

5821 gttgggatag agccagataa gaaaactaaa tattccctaa aacggctaaa gccggataac 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 5940

5881 caatttttta ctcgactaaa ttgtttttaa attgcgctta aaattgtttt ataattgcaa 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 6000

5941 atgttaaagg actacgccat aaaagaggaa tgcgtagaca cgccataaag tgtggcgtat 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat 6060

6001 ccgttcacgt gtttgttatg aatttattta tgatgagtca ttattggata aagaatcgta 6060

.......... .......... .......... .......... .......... ORF2 TRP1

.......... .......... .......... .......... .......... ..........

6061 ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc 6120

6061 aaaactgctt taaacgataa aacaatctca gaaaatgtgg taaacagagg tgtggaggcg 6120

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca 6180

6121 aatgtagttg tggttattgc ggtaaattag attcgcgtag tggttgtaaa agaccgcagt 6180

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca acccagtcag 6240

6181 caggtggtcg attgtatttt acattcgaaa gccccgagag aacggaaggt tgggtcagtc 6240

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca aacaagggaa 6300

6241 tttagctcaa ggttaggttt tcaagtggac agggtggacg aagacttagt ttgttccctt 6300

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct tttggaaata 6360

6301 atttgcttac tccaaagaca cttcgacgtg actcatcata caacgtcaga aaacctttat 6360

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac gactcatctc 6420

6361 gctcagaaaa ttattgaccg tttggctcct tgagaaccat aagaacggtg ctgagtagag 6420

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca tcctccttag 6480

6421 gtacgtcaac ctgctatagt tacggcatta gtaactggtc tcggttttgt aggaggaatc 6480

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt ttatatgctt 6540

6481 caactaatgc tttgtgcggt tggttcataa agcctcacgg acttgataaa aatatacgaa 6540

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt ctattgggca 6600

6541 aatgttctga actttaaaag gaacgttatt ggcccagtta acaagagaaa gataacccgt 6600

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 cacatataat acccagcaag tcagcatcgg aatctagagc acattctgcg gcctctgtgc 6660

6601 gtgtatatta tgggtcgttc agtcgtagcc ttagatctcg tgtaagacgc cggagacacg 6660

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta ataacagaca 6720

6661 agacgttcgg cgtttgaaag tggttacctg gtcttgatgg acactttaat tattgtctgt 6720

.......... .......... ORF2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tactccaagc tgcctttgtg tgcttaatca cgtatactca cgtgctcaat agtcaccaat 6780

6721 atgaggttcg acggaaacac acgaattagt gcatatgagt gcacgagtta tcagtggtta 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gccctccctc ttggccctct ccttttcttt tttcgaccga attaattctt aatcggcaaa 6840

6781 cgggagggag aaccgggaga ggaaaagaaa aaagctggct taattaagaa ttagccgttt 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 aaaagaaaag ctccggatca agattgtacg taaggtgaca agctattttt caataaagaa 6900

6841 ttttcttttc gaggcctagt tctaacatgc attccactgt tcgataaaaa gttatttctt 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tatcttccac tactgccatc tggcgtcata actgcaaagt acacatatat tacgatgctg 6960

6901 atagaaggtg atgacggtag accgcagtat tgacgtttca tgtgtatata atgctacgac 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tctattaaat gcttcctata ttatatatat agtaatgtcg tttatggtgc actctcagta 7020

6961 agataattta cgaaggatat aatatatata tcattacagc aaataccacg tgagagtcat 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 7080

7021 gttagacgag actacggcgt atcaattcgg tcggggctgt gggcggttgt gggcgactgc 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 7140

7081 gcgggactgc ccgaacagac gagggccgta ggcgaatgtc tgttcgacac tggcagaggc 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga.. .......... 7188

7141 cctcgacgta cacagtctcc aaaagtggca gtagtggctt tgcgcgct.. .......... 7188

pGEX_3_primer ...... .......... .......... .......... ..........