L%LOCUS pALTER-1 5680 bp DNA SYN 29-Aug-2007 DEFINITION pALTER-1 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5680 /organism="pALTER-1" /mol_type="other DNA" misc_feature complement(69..86) /label="Sp6_primer" misc_feature complement(100..118) /label="M13_reverse_primer" misc_feature complement(117..139) /label="M13_pUC_rev_primer" promoter complement(153..182) /label="lac_promoter" misc_feature complement(893..912) /label="pBRrevBam_primer" CDS 541..1731 /label="ORF frame 1" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST*" gene complement(541..1731) /label="tet" /gene="tet" misc_feature 2372..2563 /label="ROP" misc_feature complement(2557..2579) /label="pGEX_3_primer" rep_origin complement(2976..3595) /label="pBR322_origin" gene complement(3750..4606) /label="Ampicillin" /gene="Ampicillin" promoter complement(4648..4676) /label="AmpR_promoter" misc_feature complement(4835..4857) /label="pGEX_3_primer" rep_origin complement(5177..5483) /label="f1_origin" misc_feature complement(5512..5660) /label="lacZ_a" misc_feature 5626..5648 /label="M13_pUC_fwd_primer" misc_feature 5641..5657 /label="M13_forward20_primer" promoter 5664..2 /label="T7_promoter" ORIGIN 1 gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct 61 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg 121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 421 aatcagggga taacgcagga aagaacatga attaattctc atgtttgaca gcttatcatc 481 gattagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt 541 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc 601 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc 661 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca 721 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta 781 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gattctctac 841 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc 901 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc 961 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttacat 1021 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta 1081 atgcaggagt cgcataaggg agagcgccga ccgatgccct tgagagcctt caacccagtc 1141 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt 1201 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc 1261 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc 1321 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt 1381 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc 1441 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg 1501 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc 1561 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc 1621 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc 1681 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc 1741 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga 1801 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca 1861 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg 1921 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg 1981 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag 2041 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag 2101 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa 2161 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc 2221 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat 2281 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga 2341 acagaaattc ccccttacac ggaggcatca agtgaccaaa caggaaaaaa ccgcccttaa 2401 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga 2461 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg 2521 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 2581 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 2641 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 2701 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 2761 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 2821 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 2881 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 2941 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 3001 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 3061 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 3121 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 3181 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 3241 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 3301 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 3361 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 3421 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 3481 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 3541 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 3601 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 3661 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 3721 agtatatatg agtaaacttt ggctgacagt taccaatgct taatcagtga ggcacctatc 3781 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 3841 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 3901 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 3961 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 4021 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgcggcatc gtggtgtcac 4081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 4141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 4201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 4261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 4321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc 4381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 4441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 4501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 4561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 4621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 4681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 4741 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 4801 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 4861 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 4921 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 4981 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 5041 ggcgaaattg taaacgttaa tattttgtta aaattcgcgt taaatatttg ttaaatcagc 5101 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 5161 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 5221 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 5281 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg 5341 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 5401 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 5461 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc 5521 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 5581 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 5641 gtaaaacgac ggccagtgaa ttgtaatacg actcactata //0 pALTER-1 "Times New RomanC0EVSp6_pri"Times New RomanC0 T7_pro"Times New RomanC0dvM13_reverse_pri"Times New RomanCɆ0uM13_pUC_rev_pri"Times New Roman0lac_pro"Times New RomanC0ORF 1"Times New RomanCɆ0tet"Times New RomanC0} pBRrevBam_pri"Times New Roman)0D  ROP"Times New RomanC0   pGEX_3_pri"Times New RomanC0  pBR322_origin"Times New RomanCɆ0 Ampicillin"Times New Roman0(DAmpR_pro"Times New RomanC0 pGEX_3_pri"Times New RomanC09k f1_origin"Times New RomanCɆ0lacZ_a"Times New Roman0M13_pUC_fwd_pri"Times New RomanC0 M13_forward20_pri"Times New Roman* TEcoRI(5),SacI(15),KpnI(21),SmaI(23),BamHI(26),XbaI(32),SalI(38),PstI(48),HindIII(56)"Times New Roman0[ ClaI"Times New Roman0'11f EcoRV(642),NheI(684)"Times New Roman0=J__ EagI(1394),NruI(1429)"Times New Romanr0 MscI"Times New Romanm0q$-&4 AatII"Times New Roman05+!}( test"Times New Roman0 ]|T test"Times New Roman0 <T