Sequence of pALTER-1

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pALTER-1

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pALTER-1 : 5680 BP; 1321 A; 1525 C; 1511 G; 1323 T; 0 other;

EcoRI SacI KpnI,SmaI,BamHI XbaI SalI PstI HindIII

1 gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct 60

1 cccgcttaag ctcgagccat gggcccctag gagatctcag ctggacgtcc gtacgttcga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg 120

61 actcataaga tatcacagtg gatttatcga accgcattag taccagtatc gacaaaggac 120

.......... Sp6_pri .......... ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 180

121 acactttaac aataggcgag tgttaaggtg tgttgtatgc tcggccttcg tatttcacat 180

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 240

181 ttcggacccc acggattact cactcgattg agtgtaatta acgcaacgcg agtgacgggc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 300

241 gaaaggtcag ccctttggac agcacggtcg acgtaattac ttagccggtt gcgcgcccct 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 360

301 ctccgccaaa cgcataaccc gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 420

361 agcaagccga cgccgctcgc catagtcgag tgagtttccg ccattatgcc aataggtgtc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ClaI(479)

421 aatcagggga taacgcagga aagaacatga attaattctc atgtttgaca gcttatcatc 480

421 ttagtcccct attgcgtcct ttcttgtact taattaagag tacaaactgt cgaatagtag 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gattagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt 540

481 ctaatcgaaa ttacgccatc aaatagtgtc aatttaacga ttgcgtcagt ccgtggcaca 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc 600

541 tactttagat tgttacgcga gtagcagtag gagccgtggc agtgggacct acgacatccg 600

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc 660

601 tatccgaacc aatacggcca tgacggcccg gagaacgccc tatagcaggt aaggctgtcg 660

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... NheI(684) .......... .......... ..........

661 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca 720

661 tagcggtcag tgataccgca cgacgatcgc gatatacgca actacgttaa agatacgcgt 720

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta 780

721 gggcaagagc ctcgtgacag gctggcgaaa ccggcggcgg gtcaggacga gcgaagcgat 780

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gattctctac 840

781 gaacctcggt gatagctgat gcgctagtac cgctggtgtg ggcaggacac ctaagagatg 840

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc 900

841 cggcctgcgt agcaccggcc gtagtggccg cggtgtccac gccaacgacc gcggatatag 900

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc 960

901 cggctgtagt ggctacccct tctagcccga gcggtgaagc ccgagtactc gcgaacaaag 960

pBRrevBam_pri ...... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttacat 1020

961 ccgcacccat accaccgtcc ggggcaccgg ccccctgaca acccgcggta gaggaatgta 1020

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta 1080

1021 cgtggtaagg aacgccgccg ccacgagttg ccggagttgg atgatgaccc gacgaaggat 1080

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atgcaggagt cgcataaggg agagcgccga ccgatgccct tgagagcctt caacccagtc 1140

1081 tacgtcctca gcgtattccc tctcgcggct ggctacggga actctcggaa gttgggtcag 1140

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt 1200

1141 tcgaggaagg ccacccgcgc cccgtactga tagcagcggc gtgaatactg acagaagaaa 1200

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc 1260

1201 tagtacgttg agcatcctgt ccacggccgt cgcgagaccc agtaaaagcc gctcctggcg 1260

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc 1320

1261 aaagcgacct cgcgctgcta ctagccggac agcgaacgcc ataagcctta gaacgtgcgg 1320

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt 1380

1321 gagcgagttc ggaagcagtg accagggcgg tggtttgcaa agccgctctt cgtccggtaa 1380

.......... .......... ORF 1 tet .......... .......... ..........

.......... EagI(1394) .......... .......... NruI(1429) ..........

1381 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc 1440

1381 tagcggccgt accgccggct gcgcgacccg atgcagaacg accgcaagcg ctgcgctccg 1440

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg 1500

1441 acctaccgga aggggtaata ctaagaagag cgaaggccgc cgtagcccta cgggcgcaac 1500

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc 1560

1501 gtccggtacg acaggtccgt ccatctactg ctggtagtcc ctgtcgaagt tcctagcgag 1560

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc 1620

1561 cgccgagaat ggtcggattg aagctagtga cctggcgact agcagtgccg ctaaatacgg 1620

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc 1680

1621 cggagccgct cgtgtacctt gcccaaccgt acctaacatc cgcggcggga tatggaacag 1680

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc 1740

1681 acggaggggc gcaacgcagc gccacgtacc tcggcccggt ggagctggac ttaccttcgg 1740

.......... .......... ORF 1 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga 1800

1741 ccgccgtgga gcgattgcct aagtggtgag gttcttaacc tcggttagtt aagaacgcct 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca 1860

1801 cttgacactt acgcgtttgg ttgggaaccg tcttgtatag gtagcgcagg cggtagaggt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... MscI(1901) ..........

1861 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg 1920

1861 cgtcggcgtg cgccgcgtag agcccgtcgc aacccaggac cggtgcccac gcgtactagc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg 1980

1921 acgaggacag caactcctgg gccgatccga ccgccccaac ggaatgacca atcgtcttac 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag 2040

1981 ttagtggcta tgcgctcgct tgcacttcgc tgacgacgac gttttgcaga cgctggactc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag 2100

2041 gttgttgtac ttaccagaag ccaaaggcac aaagcatttc agacctttgc gccttcagtc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa 2160

2101 gcgggacgtg gtaatacaag gcctagacgt agcgtcctac gacgaccgat gggacacctt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc 2220

2161 gtggatgtag acataattgc ttcgcgaccg taactgggac tcactaaaaa gagaccaggg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat 2280

2221 cggcgtaggt atggcggtca acaaatggga gtgttgcaag gtcattggcc cgtacaagta 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga 2340

2281 gtagtcattg ggcatagcac tcgtaggaga gagcaaagta gccatagtaa tgggggtact 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acagaaattc ccccttacac ggaggcatca agtgaccaaa caggaaaaaa ccgcccttaa 2400

2341 tgtctttaag ggggaatgtg cctccgtagt tcactggttt gtcctttttt ggcgggaatt 2400

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2401 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga 2460

2401 gtaccgggcg aaatagtctt cggtctgtaa ttgcgaagac ctctttgagt tgctcgacct 2460

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg 2520

2461 gcgcctactt gtccgtctgt agacacttag cgaagtgctg gtgcgactac tcgaaatggc 2520

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 2580

2521 gtcgacggag cgcgcaaagc cactactgcc acttttggag actgtgtacg tcgagggcct 2580

.......... .......... ROP .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2581 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 2640

2581 ctgccagtgt cgaacagaca ttcgcctacg gccctcgtct gttcgggcag tcccgcgcag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 2700

2641 tcgcccacaa ccgcccacag ccccgcgtcg gtactgggtc agtgcatcgc tatcgcctca 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 2760

2701 catatgaccg aattgatacg ccgtagtctc gtctaacatg actctcacgt ggtatacgcc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 2820

2761 acactttatg gcgtgtctac gcattcctct tttatggcgt agtccgcgag aaggcgaagg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 2880

2821 agcgagtgac tgagcgacgc gagccagcaa gccgacgccg ctcgccatag tcgagtgagt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 2940

2881 ttccgccatt atgccaatag gtgtcttagt cccctattgc gtcctttctt gtacactcgt 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 3000

2941 tttccggtcg ttttccggtc cttggcattt ttccggcgca acgaccgcaa aaaggtatcc 3000

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3001 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 3060

3001 gaggcggggg gactgctcgt agtgttttta gctgcgagtt cagtctccac cgctttgggc 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 3120

3061 tgtcctgata tttctatggt ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 3180

3121 ggctgggacg gcgaatggcc tatggacagg cggaaagagg gaagcccttc gcaccgcgaa 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 3240

3181 agagtatcga gtgcgacatc catagagtca agccacatcc agcaagcgag gttcgacccg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 3300

3241 acacacgtgc ttggggggca agtcgggctg gcgacgcgga ataggccatt gatagcagaa 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 3360

3301 ctcaggttgg gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 3420

3361 tcgtctcgct ccatacatcc gccacgatgt ctcaagaact tcaccaccgg attgatgccg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 3480

3421 atgtgatctt cctgtcataa accatagacg cgagacgact tcggtcaatg gaagcctttt 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 3540

3481 tctcaaccat cgagaactag gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 3600

3541 acgttcgtcg tctaatgcgc gtcttttttt cctagagttc ttctaggaaa ctagaaaaga 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 3660

3601 tgccccagac tgcgagtcac cttgcttttg agtgcaattc cctaaaacca gtactctaat 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 3720

3661 agtttttcct agaagtggat ctaggaaaat ttaattttta cttcaaaatt tagttagatt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agtatatatg agtaaacttt ggctgacagt taccaatgct taatcagtga ggcacctatc 3780

3721 tcatatatac tcatttgaaa ccgactgtca atggttacga attagtcact ccgtggatag 3780

.......... .......... .......... .......... Ampicillin ..........

.......... .......... .......... .......... .......... ..........

3781 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 3840

3781 agtcgctaga cagataaagc aagtaggtat caacggactg aggggcagca catctattga 3840

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 3900

3841 tgctatgccc tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg 3900

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 3960

3901 agtggccgag gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca 3960

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 4020

3961 ccaggacgtt gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat 4020

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgcggcatc gtggtgtcac 4080

4021 tcatcaagcg gtcaattatc aaacgcgttg caacaacggt aacgccgtag caccacagtg 4080

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 4140

4081 cgagcagcaa accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta 4140

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 4200

4141 ctagggggta caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt 4200

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 4260

4201 cattcaaccg gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac 4260

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 4320

4261 agtacggtag gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc 4320

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc 4380

4321 ttatcacata cgccgctggc tcaacgagaa cgggccgcag ttgtgcccta ttatggcgcg 4380

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 4440

4381 gtgtatcgtc ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga 4440

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 4500

4441 gttcctagaa tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta 4500

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 4560

4501 gaagtcgtag aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac 4560

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 4620

4561 ggcgtttttt cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag 4620

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 4680

4621 ttataataac ttcgtaaata gtcccaataa cagagtactc gcctatgtat aaacttacat 4680

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 4740

4681 aaatcttttt atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac ggtggactgc 4740

.......... .......... .......... .......... .......... ..........

AatII(4741) ........ .......... .......... .......... ..........

4741 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 4800

4741 agattctttg gtaataatag tactgtaatt ggatattttt atccgcatag tgctccggga 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 4860

4801 aagcagagcg cgcaaagcca ctactgccac ttttggagac tgtgtacgtc gagggcctct 4860

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

4861 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 4920

4861 gccagtgtcg aacagacatt cgcctacggc cctcgtctgt tcgggcagtc ccgcgcagtc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 4980

4921 gcccacaacc gcccacagcc ccgaccgaat tgatacgccg tagtctcgtc taacatgact 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 5040

4981 ctcacgtggt atacgccaca ctttatggcg tgtctacgca ttcctctttt atggcgtagt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ggcgaaattg taaacgttaa tattttgtta aaattcgcgt taaatatttg ttaaatcagc 5100

5041 ccgctttaac atttgcaatt ataaaacaat tttaagcgca atttataaac aatttagtcg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 5160

5101 agtaaaaaat tggttatccg gctttagccg ttttagggaa tatttagttt tcttatctgg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 5220

5161 ctctatccca actcacaaca aggtcaaacc ttgttctcag gtgataattt cttgcacctg 5220

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 5280

5221 aggttgcagt ttcccgcttt ttggcagata gtcccgctac cgggtgatgc acttggtagt 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg 5340

5281 gggtttagtt caaaaaacgc cagctccacg gcatttcgag atttagcctt gggatttccc 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 5400

5341 tcgggggcta aatctcgaac tgcccctttc ggccgcttgc accgctcttt ccttcccttc 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 5460

5401 tttcgctttc ctcgcccgcg atcccgcgac cgttcacatc gccagtgcga cgcgcattgg 5460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc 5520

5461 tggtgtgggc ggcgcgaatt acgcggcgat gtcccgcgca ggtaagcggt aagtccgacg 5520

.......... f1_origin .......... .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

5521 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 5580

5521 cgttgacaac ccttcccgct agccacgccc ggagaagcga taatgcggtc gaccgctttc 5580

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 5640

5581 cccctacacg acgttccgct aattcaaccc attgcggtcc caaaagggtc agtgctgcaa 5640

.......... .......... lacZ_a .......... ....... M13_pUC_fwd_pri

.......... .......... .......... .......... .......... ..........

5641 gtaaaacgac ggccagtgaa ttgtaatacg actcactata .......... .......... 5680

5641 cattttgctg ccggtcactt aacattatgc tgagtgatat .......... .......... 5680

lacZ_a M13_forward20_pri ...... .......... .......... ..........