||X%LOCUS pALTER-Control 5676 bp DNA SYN 12-Sep-2007 DEFINITION pALTER-Control ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5676 /organism="pALTER-Control" /mol_type="other DNA" misc_feature complement(65..82) /label="Sp6_primer" misc_feature complement(96..114) /label="M13_reverse_primer" misc_feature complement(113..135) /label="M13_pUC_rev_primer" promoter complement(149..178) /label="lac_promoter" misc_feature complement(889..908) /label="pBRrevBam_primer" CDS 537..1727 /label="ORF frame 3" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST*" gene complement(537..1727) /label="tet" /gene="tet" misc_feature 2368..2559 /label="ROP" misc_feature complement(2553..2575) /label="pGEX_3_primer" rep_origin complement(2972..3591) /label="pBR322_origin" gene complement(3746..4602) /label="Ampicillin" /gene="Ampicillin" promoter complement(4644..4672) /label="AmpR_promoter" misc_feature complement(4831..4853) /label="pGEX_3_primer" rep_origin complement(5173..5479) /label="f1_origin" misc_feature complement(5508..5656) /label="lacZ_a" misc_feature 5622..5644 /label="M13_pUC_fwd_primer" misc_feature 5637..5653 /label="M13_forward20_primer" promoter 5660..2 /label="T7_promoter" ORIGIN 1 GGGCGAATTC GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCGGCATG CAAGCTTGAG 61 TATTCTATAG TGTCACCTAA ATAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 121 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 181 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 241 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 301 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 361 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 421 AGGGGATAAC GCAGGAAAGA ACATGAATTA ATTCTCATGT TTGACAGCTT ATCATCGATT 481 AGCTTTAATG CGGTAGTTTA TCACAGTTAA ATTGCTAACG CAGTCAGGCA CCGTGTATGA 541 AATCTAACAA TGCGCTCATC GTCATCCTCG GCACCGTCAC CCTGGATGCT GTAGGCATAG 601 GCTTGGTTAT GCCGGTACTG CCGGGCCTCT TGCGGGATAT CGTCCATTCC GACAGCATCG 661 CCAGTCACTA TGGCGTGCTG CTAGCGCTAT ATGCGTTGAT GCAATTTCTA TGCGCACCCG 721 TTCTCGGAGC ACTGTCCGAC CGCTTTGGCC GCCGCCCAGT CCTGCTCGCT TCGCTACTTG 781 GAGCCACTAT CGACTACGCG ATCATGGCGA CCACACCCGT CCTGTGGATT CTCTACGCCG 841 GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG 901 ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG 961 TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTACATGCAC 1021 CATTCCTTGC GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC 1081 AGGAGTCGCA TAAGGGAGAG CGCCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT 1141 CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA 1201 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTGGGTCAT TTTCGGCGAG GACCGCTTTC 1261 GCTGGAGCGC GACGATGATC GGCCTGTCGC TTGCGGTATT CGGAATCTTG CACGCCCTCG 1321 CTCAAGCCTT CGTCACTGGT CCCGCCACCA AACGTTTCGG CGAGAAGCAG GCCATTATCG 1381 CCGGCATGGC GGCCGACGCG CTGGGCTACG TCTTGCTGGC GTTCGCGACG CGAGGCTGGA 1441 TGGCCTTCCC CATTATGATT CTTCTCGCTT CCGGCGGCAT CGGGATGCCC GCGTTGCAGG 1501 CCATGCTGTC CAGGCAGGTA GATGACGACC ATCAGGGACA GCTTCAAGGA TCGCTCGCGG 1561 CTCTTACCAG CCTAACTTCG ATCACTGGAC CGCTGATCGT CACGGCGATT TATGCCGCCT 1621 CGGCGAGCAC ATGGAACGGG TTGGCATGGA TTGTAGGCGC CGCCCTATAC CTTGTCTGCC 1681 TCCCCGCGTT GCGTCGCGGT GCATGGAGCC GGGCCACCTC GACCTGAATG GAAGCCGGCG 1741 GCACCTCGCT AACGGATTCA CCACTCCAAG AATTGGAGCC AATCAATTCT TGCGGAGAAC 1801 TGTGAATGCG CAAACCAACC CTTGGCAGAA CATATCCATC GCGTCCGCCA TCTCCAGCAG 1861 CCGCACGCGG CGCATCTCGG GCAGCGTTGG GTCCTGGCCA CGGGTGCGCA TGATCGTGCT 1921 CCTGTCGTTG AGGACCCGGC TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC AGAATGAATC 1981 ACCGATACGC GAGCGAACGT GAAGCGACTG CTGCTGCAAA ACGTCTGCGA CCTGAGCAAC 2041 AACATGAATG GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG GAAACGCGGA AGTCAGCGCC 2101 CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC TGGCTACCCT GTGGAACACC 2161 TACATCTGTA TTAACGAAGC GCTGGCATTG ACCCTGAGTG ATTTTTCTCT GGTCCCGCCG 2221 CATCCATACC GCCAGTTGTT TACCCTCACA ACGTTCCAGT AACCGGGCAT GTTCATCATC 2281 AGTAACCCGT ATCGTGAGCA TCCTCTCTCG TTTCATCGGT ATCATTACCC CCATGAACAG 2341 AAATTCCCCC TTACACGGAG GCATCAAGTG ACCAAACAGG AAAAAACCGC CCTTAACATG 2401 GCCCGCTTTA TCAGAAGCCA GACATTAACG CTTCTGGAGA AACTCAACGA GCTGGACGCG 2461 GATGAACAGG CAGACATCTG TGAATCGCTT CACGACCACG CTGATGAGCT TTACCGCAGC 2521 TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 2581 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 2641 GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT 2701 ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 2761 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC 2821 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 2881 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 2941 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 3001 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 3061 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 3121 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 3181 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 3241 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 3301 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 3361 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 3421 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 3481 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 3541 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 3601 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 3661 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 3721 TATATGAGTA AACTTTGGCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 3781 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 3841 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 3901 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 3961 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 4021 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC GGCATCGTGG TGTCACGCTC 4081 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 4141 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 4201 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 4261 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 4321 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA 4381 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 4441 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 4501 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 4561 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 4621 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 4681 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA 4741 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG 4801 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 4861 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 4921 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 4981 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 5041 AAATTGTAAA CGTTAATATT TTGTTAAAAT TCGCGTTAAA TATTTGTTAA ATCAGCTCAT 5101 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA 5161 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA 5221 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCA 5281 AATCAAGTTT TTTGCGGTCG AGGTGCCGTA AAGCTCTAAA TCGGAACCCT AAAGGGAGCC 5341 CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG 5401 CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA 5461 CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCAT TCGCCATTCA GGCTGCGCAA 5521 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG 5581 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA 5641 AACGACGGCC AGTGAATTGT AATACGACTC ACTATA //,pALTER-Control "Times New Roman|C,ARSp6_pri"Times New Roman|C,T7_pro"Times New Roman|C,`rM13_reverse_pri"Times New Roman|CɆ,qM13_pUC_rev_pri"Times New Roman|,lac_pro"Times New Roman|C,ORF 3"Times New Roman|CɆ,tet"Times New Roman|C,y pBRrevBam_pri"Times New Roman)|,@ ROP"Times New Roman|C,   pGEX_3_pri"Times New Roman|C,  pBR322_origin"Times New Roman|CɆ, Ampicillin"Times New Roman|,$@AmpR_pro"Times New Roman|C, pGEX_3_pri"Times New Roman|C,5g f1_origin"Times New Roman|CɆ,lacZ_a"Times New Roman|, M13_pUC_fwd_pri"Times New Roman|C,M13_forward20_pri"Times New Roman* |KEcoRI(5),SacI(15),KpnI(21),SmaI(23),BamHI(26),XbaI(32),SalI(38),HindIII(52)"Times New Roman ,ZL |ClaI"Times New Roman, **_ |EcoRV(638),NheI(680)"Times New Roman~,6C[[ |EagI(1390),NruI(1425)"Times New Romann,} |MscI"Times New Romani,j{" |AatII"Times New Roman,#v |test"Times New Roman~,ToS |test"Times New Roman,w.!S