Sequence of pALTER-Control

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Sequence of pALTER-Control

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Sequence of pALTER-Control : 5676 BP; 1320 A; 1524 C; 1510 G; 1322 T; 0 other;

EcoRI SacI KpnI,SmaI,BamHI XbaI SalI) HindIII(52)

1 gggcgaattc gagctcggta cccggggatc ctctagagtc gaccggcatg caagcttgag 60

1 cccgcttaag ctcgagccat gggcccctag gagatctcag ctggccgtac gttcgaactc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 120

61 ataagatatc acagtggatt tatcgaaccg cattagtacc agtatcgaca aaggacacac 120

.......... Sp6_pri .......... ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

121 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 180

121 tttaacaata ggcgagtgtt aaggtgtgtt gtatgctcgg ccttcgtatt tcacatttcg 180

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

181 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 240

181 gaccccacgg attactcact cgattgagtg taattaacgc aacgcgagtg acgggcgaaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 300

241 ggtcagccct ttggacagca cggtcgacgt aattacttag ccggttgcgc gcccctctcc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 360

301 gccaaacgca taacccgcga gaaggcgaag gagcgagtga ctgagcgacg cgagccagca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 420

361 agccgacgcc gctcgccata gtcgagtgag tttccgccat tatgccaata ggtgtcttag 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ClaI(475)

421 aggggataac gcaggaaaga acatgaatta attctcatgt ttgacagctt atcatcgatt 480

421 tcccctattg cgtcctttct tgtacttaat taagagtaca aactgtcgaa tagtagctaa 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga 540

481 tcgaaattac gccatcaaat agtgtcaatt taacgattgc gtcagtccgt ggcacatact 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag 600

541 ttagattgtt acgcgagtag cagtaggagc cgtggcagtg ggacctacga catccgtatc 600

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg 660

601 cgaaccaata cggccatgac ggcccggaga acgccctata gcaggtaagg ctgtcgtagc 660

.......... .......... ORF 3 tet .......... .......... ..........

.......... NheI(680) .......... .......... .......... ..........

661 ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg 720

661 ggtcagtgat accgcacgac gatcgcgata tacgcaacta cgttaaagat acgcgtgggc 720

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg 780

721 aagagcctcg tgacaggctg gcgaaaccgg cggcgggtca ggacgagcga agcgatgaac 780

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatt ctctacgccg 840

781 ctcggtgata gctgatgcgc tagtaccgct ggtgtgggca ggacacctaa gagatgcggc 840

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 900

841 ctgcgtagca ccggccgtag tggccgcggt gtccacgcca acgaccgcgg atatagcggc 900

.......... .......... ORF 3 tet .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

901 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 960

901 tgtagtggct accccttcta gcccgagcgg tgaagcccga gtactcgcga acaaagccgc 960

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttacatgcac 1020

961 acccatacca ccgtccgggg caccggcccc ctgacaaccc gcggtagagg aatgtacgtg 1020

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 1080

1021 gtaaggaacg ccgccgccac gagttgccgg agttggatga tgacccgacg aaggattacg 1080

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aggagtcgca taagggagag cgccgaccga tgcccttgag agccttcaac ccagtcagct 1140

1081 tcctcagcgt attccctctc gcggctggct acgggaactc tcggaagttg ggtcagtcga 1140

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca 1200

1141 ggaaggccac ccgcgccccg tactgatagc agcggcgtga atactgacag aagaaatagt 1200

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc 1260

1201 acgttgagca tcctgtccac ggccgtcgcg agacccagta aaagccgctc ctggcgaaag 1260

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg 1320

1261 cgacctcgcg ctgctactag ccggacagcg aacgccataa gccttagaac gtgcgggagc 1320

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg 1380

1321 gagttcggaa gcagtgacca gggcggtggt ttgcaaagcc gctcttcgtc cggtaatagc 1380

.......... .......... ORF 3 tet .......... .......... ..........

EagI(1390) .......... .......... .......... NruI(1425) ..........

1381 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga 1440

1381 ggccgtaccg ccggctgcgc gacccgatgc agaacgaccg caagcgctgc gctccgacct 1440

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg 1500

1441 accggaaggg gtaatactaa gaagagcgaa ggccgccgta gccctacggg cgcaacgtcc 1500

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg 1560

1501 ggtacgacag gtccgtccat ctactgctgg tagtccctgt cgaagttcct agcgagcgcc 1560

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct 1620

1561 gagaatggtc ggattgaagc tagtgacctg gcgactagca gtgccgctaa atacggcgga 1620

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc 1680

1621 gccgctcgtg taccttgccc aaccgtacct aacatccgcg gcgggatatg gaacagacgg 1680

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg 1740

1681 aggggcgcaa cgcagcgcca cgtacctcgg cccggtggag ctggacttac cttcggccgc 1740

.......... .......... ORF 3 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac 1800

1741 cgtggagcga ttgcctaagt ggtgaggttc ttaacctcgg ttagttaaga acgcctcttg 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag 1860

1801 acacttacgc gtttggttgg gaaccgtctt gtataggtag cgcaggcggt agaggtcgtc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... MscI(1897) .......... ..........

1861 ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct 1920

1861 ggcgtgcgcc gcgtagagcc cgtcgcaacc caggaccggt gcccacgcgt actagcacga 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc 1980

1921 ggacagcaac tcctgggccg atccgaccgc cccaacggaa tgaccaatcg tcttacttag 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac 2040

1981 tggctatgcg ctcgcttgca cttcgctgac gacgacgttt tgcagacgct ggactcgttg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc 2100

2041 ttgtacttac cagaagccaa aggcacaaag catttcagac ctttgcgcct tcagtcgcgg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc 2160

2101 gacgtggtaa tacaaggcct agacgtagcg tcctacgacg accgatggga caccttgtgg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg 2220

2161 atgtagacat aattgcttcg cgaccgtaac tgggactcac taaaaagaga ccagggcggc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc 2280

2221 gtaggtatgg cggtcaacaa atgggagtgt tgcaaggtca ttggcccgta caagtagtag 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag 2340

2281 tcattgggca tagcactcgt aggagagagc aaagtagcca tagtaatggg ggtacttgtc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg 2400

2341 tttaaggggg aatgtgcctc cgtagttcac tggtttgtcc ttttttggcg ggaattgtac 2400

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2401 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg 2460

2401 cgggcgaaat agtcttcggt ctgtaattgc gaagacctct ttgagttgct cgacctgcgc 2460

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc 2520

2461 ctacttgtcc gtctgtagac acttagcgaa gtgctggtgc gactactcga aatggcgtcg 2520

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 2580

2521 acggagcgcg caaagccact actgccactt ttggagactg tgtacgtcga gggcctctgc 2580

.......... ROP .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2581 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 2640

2581 cagtgtcgaa cagacattcg cctacggccc tcgtctgttc gggcagtccc gcgcagtcgc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 2700

2641 ccacaaccgc ccacagcccc gcgtcggtac tgggtcagtg catcgctatc gcctcacata 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 2760

2701 tgaccgaatt gatacgccgt agtctcgtct aacatgactc tcacgtggta tacgccacac 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 2820

2761 tttatggcgt gtctacgcat tcctctttta tggcgtagtc cgcgagaagg cgaaggagcg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 2880

2821 agtgactgag cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 2940

2881 gccattatgc caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 3000

2941 cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg 3000

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3001 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3060

3001 cggggggact gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 3120

3061 ctgatatttc tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 3180

3121 gggacggcga atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 3240

3181 tatcgagtgc gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 3300

3241 acgtgcttgg ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 3360

3301 ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3420

3361 ctcgctccat acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 3480

3421 gatcttcctg tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 3540

3481 aaccatcgag aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 3600

3541 tcgtcgtcta atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 3660

3601 ccagactgcg agtcaccttg cttttgagtg caattcccta aaaccagtac tctaatagtt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 3720

3661 tttcctagaa gtggatctag gaaaatttaa tttttacttc aaaatttagt tagatttcat 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tatatgagta aactttggct gacagttacc aatgcttaat cagtgaggca cctatctcag 3780

3721 atatactcat ttgaaaccga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc 3780

.......... .......... .......... .......... Ampicillin ..........

.......... .......... .......... .......... .......... ..........

3781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 3840

3781 gctagacaga taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct 3840

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 3900

3841 atgccctccc gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg 3900

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 3960

3901 gccgaggtct aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag 3960

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 4020

3961 gacgttgaaa taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat 4020

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc ggcatcgtgg tgtcacgctc 4080

4021 caagcggtca attatcaaac gcgttgcaac aacggtaacg ccgtagcacc acagtgcgag 4080

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 4140

4081 cagcaaacca taccgaagta agtcgaggcc aagggttgct agttccgctc aatgtactag 4140

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 4200

4141 ggggtacaac acgttttttc gccaatcgag gaagccagga ggctagcaac agtcttcatt 4200

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 4260

4201 caaccggcgt cacaatagtg agtaccaata ccgtcgtgac gtattaagag aatgacagta 4260

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 4320

4261 cggtaggcat tctacgaaaa gacactgacc actcatgagt tggttcagta agactcttat 4320

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 4380

4321 cacatacgcc gctggctcaa cgagaacggg ccgcagttgt gccctattat ggcgcggtgt 4380

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 4440

4381 atcgtcttga aattttcacg agtagtaacc ttttgcaaga agccccgctt ttgagagttc 4440

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 4500

4441 ctagaatggc gacaactcta ggtcaagcta cattgggtga gcacgtgggt tgactagaag 4500

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 4560

4501 tcgtagaaaa tgaaagtggt cgcaaagacc cactcgtttt tgtccttccg ttttacggcg 4560

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 4620

4561 ttttttccct tattcccgct gtgcctttac aacttatgag tatgagaagg aaaaagttat 4620

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 4680

4621 aataacttcg taaatagtcc caataacaga gtactcgcct atgtataaac ttacataaat 4680

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... ......... AatII(4737)

4681 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 4740

4681 ctttttattt gtttatcccc aaggcgcgtg taaaggggct tttcacggtg gactgcagat 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 4800

4741 tctttggtaa taatagtact gtaattggat atttttatcc gcatagtgct ccgggaaagc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 4860

4801 agagcgcgca aagccactac tgccactttt ggagactgtg tacgtcgagg gcctctgcca 4860

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

4861 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 4920

4861 gtgtcgaaca gacattcgcc tacggccctc gtctgttcgg gcagtcccgc gcagtcgccc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 4980

4921 acaaccgccc acagccccga ccgaattgat acgccgtagt ctcgtctaac atgactctca 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 5040

4981 cgtggtatac gccacacttt atggcgtgtc tacgcattcc tcttttatgg cgtagtccgc 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tatttgttaa atcagctcat 5100

5041 tttaacattt gcaattataa aacaatttta agcgcaattt ataaacaatt tagtcgagta 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 5160

5101 aaaaattggt tatccggctt tagccgtttt agggaatatt tagttttctt atctggctct 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 5220

5161 atcccaactc acaacaaggt caaaccttgt tctcaggtga taatttcttg cacctgaggt 5220

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5221 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca 5280

5221 tgcagtttcc cgctttttgg cagatagtcc cgctaccggg tgatgcactt ggtagtgggt 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct aaagggagcc 5340

5281 ttagttcaaa aaacgccagc tccacggcat ttcgagattt agccttggga tttccctcgg 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 5400

5341 gggctaaatc tcgaactgcc cctttcggcc gcttgcaccg ctctttcctt cccttctttc 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 5460

5401 gctttcctcg cccgcgatcc cgcgaccgtt cacatcgcca gtgcgacgcg cattggtggt 5460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa 5520

5461 gtgggcggcg cgaattacgc ggcgatgtcc cgcgcaggta agcggtaagt ccgacgcgtt 5520

f1_origin .......... .......... .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

5521 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 5580

5521 gacaaccctt cccgctagcc acgcccggag aagcgataat gcggtcgacc gctttccccc 5580

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 5640

5581 tacacgacgt tccgctaatt caacccattg cggtcccaaa agggtcagtg ctgcaacatt 5640

.......... .......... lacZ_a .......... ....... M13_pUC_fwd_pri

.......... .......... .......... .......... .......... ..........

5641 aacgacggcc agtgaattgt aatacgactc actata.... .......... .......... 5676

5641 ttgctgccgg tcacttaaca ttatgctgag tgatat.... .......... .......... 5676

lacZ_a M13_forward20_pri ...... .......... .......... ..........