+LOCUS CVU47102 5858 bp DNA circular SYN 19-FEB-1996 DEFINITION Cloning vector pALTER-Ex1, complete sequence. ACCESSION U47102 NID g1197548 KEYWORDS . SOURCE Cloning vector pALTER-Ex1. ORGANISM Cloning vector pALTER-Ex1 artificial sequence; cloning vectors. REFERENCE 1 (bases 1 to 5858) AUTHORS Lesley,S. and Bohnsack,R. TITLE Direct Submission JOURNAL Submitted (24-JAN-1996) Melinda Brady, Production, Promega Corporation, 5445 East Cheryl Parkway, Madison, WI 53711, USA COMMENT NCBI gi: 1197548 FEATURES Location/Qualifiers source 1..5858 /organism="Cloning vector pALTER-Ex1" misc_feature 1 /note="T7 RNA polymerase transcription initiation site" misc_feature 5..77 /note="multiple cloning site" misc_feature 37..39 /note="T7 translation start codon" misc_feature group(65..67,71..73) /note="tac and SP6 translation start codons" misc_feature 109 /note="tac transcription initiation site" promoter 120..147 /note="tac promoter" promoter 172..194 /note="SP6 RNA polymerase promoter" misc_feature 177 /note="SP6 RNA polymerase transcription initiation site" primer_bind 212..228 /note="pUC/M13 reverse sequencing primer" CDS 719..1909 /gene="tetR" /note="NCBI gi: 1197549" /codon_start=1 /transl_table=11 /product="TetR" /db_xref="PID:g1197549" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" misc_binding 801..828 /gene="tetR" /bound_moiety="tetracycline repair oligonucleotide" CDS complement(4212..4784) /gene="ampR" /note="NCBI gi: 1197550" /codon_start=1 /function="ampicillin resistance" /transl_table=11 /product="beta-lactamase" /db_xref="PID:g1197550" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPQWQQRCANY" misc_binding 4232..4251 /gene="ampR" /bound_moiety="ampicillin repair oligonucleotide" misc_feature 5223..5678 /note="phage f1 region" primer_bind 5794..5810 /note="pUC/M13 forward sequencing primer" promoter join(5842..5858,1..6) /note="T7 RNA polymerase promoter" BASE COUNT 1363 a 1560 c 1553 g 1382 t ORIGIN 1 gggcgaattc gttaacttta agaaggagat atacccatgg aggcctctag actgcagcgg 61 ccgcatgcat atggatcctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 121 ttatacgagc cgatgattaa ttgtcaacag ggggatgggg agtaagcttg agtattctat 181 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 241 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 301 cctaatgagt gagctaacca cattaatttg cgttgcgctc actgcccgct ttccagtcgg 361 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 541 acgcaggaaa gaacatgaat taattctcat gtttgacagc ttatcatcgg ataacccctt 601 ggggcctcta aacgggtctt gaggggtttt ttgttctcat gtttgacagc ttatcttcga 661 ttagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat 721 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat 781 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat 841 cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc 901 cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact 961 tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga ttctctacgc 1021 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc 1081 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg 1141 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttacatgc 1201 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat 1261 gcaggagtcg cataagggag agcgccgacc gatgcccttg agagccttca acccagtcag 1321 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat 1381 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt 1441 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct 1501 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat 1561 cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg 1621 gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca 1681 ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc 1741 ggctcttacc agcctaactt cgatcactgg accgctgatc gtcacggcga tttatgccgc 1801 ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg 1861 cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg 1921 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga 1981 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc 2041 agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg 2101 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa 2161 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca 2221 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg 2281 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca 2341 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc 2401 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca 2461 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac 2521 agaaattccc ccttacacgg aggcatcaag tgaccaaaca ggaaaaaacc gcccttaaca 2581 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg 2641 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca 2701 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 2761 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 2821 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 2881 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc ctatgcggtg 2941 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 3001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 3481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 3541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 3601 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 3661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 3721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 3781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 3841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 3901 tatatatgag taaactttgg ctgacagtta ccaatgctta atcagtgagg cacctatctc 3961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gcggcatcgt ggtgtcacgc 4261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 4321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 4381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 4441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 4501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 4561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 4681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 4741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 4801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 4861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 4921 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 4981 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 5041 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 5101 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 5161 gtgcacccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 5221 cgaaattgta aacgttaata ttttgttaaa attcgcgtta aatatttgtt aaatcagctc 5281 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 5341 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 5401 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 5461 caaatcaagt tttttgcggt cgaggtgccg taaagctcta aatcggaacc ctaaagggag 5521 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 5581 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 5641 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc 5701 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 5761 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 5821 aaaacgacgg ccagtgaatt gtaatacgac tcactata //  pALTER-EX1 "Times New RomanC T7_transl_en_RBS"Times New RomanCT7_pro"Times New RomanCTjM13_pUC_rev_pri"Times New RomanCɆvtac_pro"Times New RomanSp6_pri"Times New RomanCM13_reverse_pri"Times New RomanCM13_pUC_rev_pri"Times New RomanCɆ"lac_pro"Times New RomanOrT7_term"Times New RomanCuORF 2"Times New Roman(CɆutet"Times New RomanC/B pBRrevBam_pri"Times New Roman ROP"Times New RomanC  pGEX_3_pri"Times New RomanCP  pBR322_origin"Times New RomanCɆV Ampicillin"Times New RomanAmpR_pro"Times New RomanC pGEX_3_pri"Times New RomanC f1_origin"Times New RomanCɆ6lacZ_a"Times New RomanM13_pUC_fwd_pri"Times New RomanCM13_forward20_pri"Times New Roman* QEcoRI(5),HpaI(13),NcoI(35),StuI(43),XbaI(46),PstI(56),NotI(58),NdeI(69),BamHI(73)"Times New Roman^^ HindIII"Times New Romanm EcoRV(820),NheI(862)"Times New Roman4I&Xg g  NruI"Times New RomanG| MscI"Times New RomanPD`Po\o\k AatII"Times New Roman5@5*q* test"Times New Roman(;M test"Times New Roman}|gooD\