Sequence of pALTER-EX1

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Sequence of pALTER-EX1

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Sequence of pALTER-EX1 : 5858 BP; 1363 A; 1560 C; 1553 G; 1382 T; 0 other;

EcoRI(5) HpaI(13) ...... NcoI(35) StuI(43),XbaI(46) ......

1 gggcgaattc gttaacttta agaaggagat atacccatgg aggcctctag actgcagcgg 60

1 cccgcttaag caattgaaat tcttcctcta tatgggtacc tccggagatc tgacgtcgcc 60

....... T7_transl_en_RBS ....... .......... .......... ..........

...... NdeI(69),BamHI(73) ...... .......... .......... ..........

61 ccgcatgcat atggatcctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 120

61 ggcgtacgta tacctaggac aaaggacaca ctttaacaat aggcgagtgt taaggtgtgt 120

.......... .......... ....... M13_pUC_rev_pri ....... ..........

.......... .......... .......... ......... HindIII(164) .........

121 ttatacgagc cgatgattaa ttgtcaacag ggggatgggg agtaagcttg agtattctat 180

121 aatatgctcg gctactaatt aacagttgtc cccctacccc tcattcgaac tcataagata 180

.......... tac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 240

181 tcacagtgga tttatcgaac cgcattagta ccagtatcga caaaggacac actttaacaa 240

Sp6_pri .......... ....... M13_reverse_pri .... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

241 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 300

241 taggcgagtg ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt cggaccccac 300

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctaatgagt gagctaacca cattaatttg cgttgcgctc actgcccgct ttccagtcgg 360

301 ggattactca ctcgattggt gtaattaaac gcaacgcgag tgacgggcga aaggtcagcc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 420

361 ctttggacag cacggtcgac gtaattactt agccggttgc gcgcccctct ccgccaaacg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 480

421 cataacccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 540

481 ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 acgcaggaaa gaacatgaat taattctcat gtttgacagc ttatcatcgg ataacccctt 600

541 tgcgtccttt cttgtactta attaagagta caaactgtcg aatagtagcc tattggggaa 600

.......... .......... .......... .......... .......... T7_term

.......... .......... .......... .......... .......... ..........

601 ggggcctcta aacgggtctt gaggggtttt ttgttctcat gtttgacagc ttatcttcga 660

601 ccccggagat ttgcccagaa ctccccaaaa aacaagagta caaactgtcg aatagaagct 660

.......... T7_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat 720

661 aatcgaaatt acgccatcaa atagtgtcaa tttaacgatt gcgtcagtcc gtggcacata 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat 780

721 ctttagattg ttacgcgagt agcagtagga gccgtggcag tgggacctac gacatccgta 780

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat 840

781 tccgaaccaa tacggccatg acggcccgga gaacgcccta tagcaggtaa ggctgtcgta 840

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... NheI(862) .......... .......... ..........

841 cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc 900

841 gcggtcagtg ataccgcacg acgatcgcga tatacgcaac tacgttaaag atacgcgtgg 900

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact 960

901 gcaagagcct cgtgacaggc tggcgaaacc ggcggcgggt caggacgagc gaagcgatga 960

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga ttctctacgc 1020

961 acctcggtga tagctgatgc gctagtaccg ctggtgtggg caggacacct aagagatgcg 1020

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc 1080

1021 gcctgcgtag caccggccgt agtggccgcg gtgtccacgc caacgaccgc ggatatagcg 1080

.......... .......... ORF 2 tet .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

1081 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg 1140

1081 gctgtagtgg ctaccccttc tagcccgagc ggtgaagccc gagtactcgc gaacaaagcc 1140

pBRrevBam_pri ...... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttacatgc 1200

1141 gcacccatac caccgtccgg ggcaccggcc ccctgacaac ccgcggtaga ggaatgtacg 1200

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat 1260

1201 tggtaaggaa cgccgccgcc acgagttgcc ggagttggat gatgacccga cgaaggatta 1260

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcaggagtcg cataagggag agcgccgacc gatgcccttg agagccttca acccagtcag 1320

1261 cgtcctcagc gtattccctc tcgcggctgg ctacgggaac tctcggaagt tgggtcagtc 1320

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat 1380

1321 gaggaaggcc acccgcgccc cgtactgata gcagcggcgt gaatactgac agaagaaata 1380

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt 1440

1381 gtacgttgag catcctgtcc acggccgtcg cgagacccag taaaagccgc tcctggcgaa 1440

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct 1500

1441 agcgacctcg cgctgctact agccggacag cgaacgccat aagccttaga acgtgcggga 1500

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat 1560

1501 gcgagttcgg aagcagtgac cagggcggtg gtttgcaaag ccgctcttcg tccggtaata 1560

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... NruI(1607) ..........

1561 cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg 1620

1561 gcggccgtac cgccggctgc gcgacccgat gcagaacgac cgcaagcgct gcgctccgac 1620

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca 1680

1621 ctaccggaag gggtaatact aagaagagcg aaggccgccg tagccctacg ggcgcaacgt 1680

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc 1740

1681 ccggtacgac aggtccgtcc atctactgct ggtagtccct gtcgaagttc ctagcgagcg 1740

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggctcttacc agcctaactt cgatcactgg accgctgatc gtcacggcga tttatgccgc 1800

1741 ccgagaatgg tcggattgaa gctagtgacc tggcgactag cagtgccgct aaatacggcg 1800

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg 1860

1801 gagccgctcg tgtaccttgc ccaaccgtac ctaacatccg cggcgggata tggaacagac 1860

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg 1920

1861 ggaggggcgc aacgcagcgc cacgtacctc ggcccggtgg agctggactt accttcggcc 1920

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga 1980

1921 gccgtggagc gattgcctaa gtggtgaggt tcttaacctc ggttagttaa gaacgcctct 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc 2040

1981 tgacacttac gcgtttggtt gggaaccgtc ttgtataggt agcgcaggcg gtagaggtcg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... MscI(2079) .......... ..........

2041 agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg 2100

2041 tcggcgtgcg ccgcgtagag cccgtcgcaa cccaggaccg gtgcccacgc gtactagcac 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa 2160

2101 gaggacagca actcctgggc cgatccgacc gccccaacgg aatgaccaat cgtcttactt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca 2220

2161 agtggctatg cgctcgcttg cacttcgctg acgacgacgt tttgcagacg ctggactcgt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg 2280

2221 tgttgtactt accagaagcc aaaggcacaa agcatttcag acctttgcgc cttcagtcgc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca 2340

2281 gggacgtggt aatacaaggc ctagacgtag cgtcctacga cgaccgatgg gacaccttgt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc 2400

2341 ggatgtagac ataattgctt cgcgaccgta actgggactc actaaaaaga gaccagggcg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca 2460

2401 gcgtaggtat ggcggtcaac aaatgggagt gttgcaaggt cattggcccg tacaagtagt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac 2520

2461 agtcattggg catagcactc gtaggagaga gcaaagtagc catagtaatg ggggtacttg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agaaattccc ccttacacgg aggcatcaag tgaccaaaca ggaaaaaacc gcccttaaca 2580

2521 tctttaaggg ggaatgtgcc tccgtagttc actggtttgt ccttttttgg cgggaattgt 2580

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2581 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg 2640

2581 accgggcgaa atagtcttcg gtctgtaatt gcgaagacct ctttgagttg ctcgacctgc 2640

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca 2700

2641 gcctacttgt ccgtctgtag acacttagcg aagtgctggt gcgactactc gaaatggcgt 2700

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 2760

2701 cgacggagcg cgcaaagcca ctactgccac ttttggagac tgtgtacgtc gagggcctct 2760

.......... ROP .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2761 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 2820

2761 gccagtgtcg aacagacatt cgcctacggc cctcgtctgt tcgggcagtc ccgcgcagtc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 2880

2821 gcccacaacc gcccacagcc ccgcgtcggt actgggtcag tgcatcgcta tcgcctcaca 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc ctatgcggtg 2940

2881 tatgaccgaa ttgatacgcc gtagtctcgt ctaacatgac tctcacgtgg gatacgccac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 3000

2941 actttatggc gtgtctacgc attcctcttt tatggcgtag tccgcgagaa ggcgaaggag 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3060

3001 cgagtgactg agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3120

3061 ccgccattat gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3180

3121 tccggtcgtt ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga 3180

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3240

3181 ggcgggggga ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3300

3241 tcctgatatt tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3360

3301 ctgggacggc gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3420

3361 agtatcgagt gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 3480

3421 acacgtgctt ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 3540

3481 caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 3600

3541 gtctcgctcc atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 3660

3601 gtgatcttcc tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 3720

3661 tcaaccatcg agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 3780

3721 gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 3840

3781 ccccagactg cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 3900

3841 tttttcctag aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tatatatgag taaactttgg ctgacagtta ccaatgctta atcagtgagg cacctatctc 3960

3901 atatatactc atttgaaacc gactgtcaat ggttacgaat tagtcactcc gtggatagag 3960

.......... .......... .......... .......... Ampicillin ..........

.......... .......... .......... .......... .......... ..........

3961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4020

3961 tcgctagaca gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg 4020

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4080

4021 ctatgccctc ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag 4080

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4140

4081 tggccgaggt ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc 4140

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4200

4141 aggacgttga aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc 4200

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gcggcatcgt ggtgtcacgc 4260

4201 atcaagcggt caattatcaa acgcgttgca acaacggtaa cgccgtagca ccacagtgcg 4260

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 4320

4261 agcagcaaac cataccgaag taagtcgagg ccaagggttg ctagttccgc tcaatgtact 4320

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 4380

4321 agggggtaca acacgttttt tcgccaatcg aggaagccag gaggctagca acagtcttca 4380

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 4440

4381 ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg acgtattaag agaatgacag 4440

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 4500

4441 tacggtaggc attctacgaa aagacactga ccactcatga gttggttcag taagactctt 4500

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 4560

4501 atcacatacg ccgctggctc aacgagaacg ggccgcagtt gtgccctatt atggcgcggt 4560

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4620

4561 gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa gaagccccgc ttttgagagt 4620

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 4680

4621 tcctagaatg gcgacaactc taggtcaagc tacattgggt gagcacgtgg gttgactaga 4680

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 4740

4681 agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt tttgtccttc cgttttacgg 4740

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 4800

4741 cgttttttcc cttattcccg ctgtgccttt acaacttatg agtatgagaa ggaaaaagtt 4800

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 4860

4801 ataataactt cgtaaatagt cccaataaca gagtactcgc ctatgtataa acttacataa 4860

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... ......... AatII(4917)

4861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 4920

4861 atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg cttttcacgg tggactgcag 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 4980

4921 attctttggt aataatagta ctgtaattgg atatttttat ccgcatagtg ctccgggaaa 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 5040

4981 gcagagcgcg caaagccact actgccactt ttggagactg tgtacgtcga gggcctctgc 5040

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

5041 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 5100

5041 cagtgtcgaa cagacattcg cctacggccc tcgtctgttc gggcagtccc gcgcagtcgc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 5160

5101 ccacaaccgc ccacagcccc gaccgaattg atacgccgta gtctcgtcta acatgactct 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtgcacccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 5220

5161 cacgtgggat acgccacact ttatggcgtg tctacgcatt cctcttttat ggcgtagtcc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgaaattgta aacgttaata ttttgttaaa attcgcgtta aatatttgtt aaatcagctc 5280

5221 gctttaacat ttgcaattat aaaacaattt taagcgcaat ttataaacaa tttagtcgag 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 5340

5281 taaaaaattg gttatccggc tttagccgtt ttagggaata tttagttttc ttatctggct 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 5400

5341 ctatcccaac tcacaacaag gtcaaacctt gttctcaggt gataatttct tgcacctgag 5400

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 5460

5401 gttgcagttt cccgcttttt ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg 5460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 caaatcaagt tttttgcggt cgaggtgccg taaagctcta aatcggaacc ctaaagggag 5520

5461 gtttagttca aaaaacgcca gctccacggc atttcgagat ttagccttgg gatttccctc 5520

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 5580

5521 gggggctaaa tctcgaactg cccctttcgg ccgcttgcac cgctctttcc ttcccttctt 5580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 5640

5581 tcgctttcct cgcccgcgat cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg 5640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc 5700

5641 gtgtgggcgg cgcgaattac gcggcgatgt cccgcgcagg taagcggtaa gtccgacgcg 5700

f1_origin .......... .......... .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

5701 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 5760

5701 ttgacaaccc ttcccgctag ccacgcccgg agaagcgata atgcggtcga ccgctttccc 5760

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 5820

5761 cctacacgac gttccgctaa ttcaacccat tgcggtccca aaagggtcag tgctgcaaca 5820

.......... .......... lacZ_a ....... M13_pUC_fwd_pri .......

.......... .......... .......... .......... .......... ..........

5821 aaaacgacgg ccagtgaatt gtaatacgac tcactata.. .......... .......... 5858

5821 ttttgctgcc ggtcacttaa cattatgctg agtgatat.. .......... .......... 5858

lacZ_a M13_forward20_pri ...... .......... .......... ..........