Sequence of pALTER-EX2

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Sequence of pALTER-EX2

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Sequence of pALTER-EX2 : 5838 BP; 1370 A; 1537 C; 1526 G; 1405 T; 0 other;

EcoRI(5) HpaI(13) .......... NcoI(35) StuI(43) ..........

1 gggcgaattc gttaacttta agaaggagat atacccatgg aggcctctag actgcagcgg 60

1 cccgcttaag caattgaaat tcttcctcta tatgggtacc tccggagatc tgacgtcgcc 60

....... T7_transl_en_RBS ....... .......... .......... ..........

...... NdeI(69),BamHI(73) ...... .......... .......... ..........

61 ccgcatgcat atggatcctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 120

61 ggcgtacgta tacctaggac aaaggacaca ctttaacaat aggcgagtgt taaggtgtgt 120

.......... .......... ....... M13_pUC_rev_pri ....... ..........

.......... .......... .......... ......... HindIII(164) .........

121 ttatacgagc cgatgattaa ttgtcaacag ggggatgggg agtaagcttg agtattctat 180

121 aatatgctcg gctactaatt aacagttgtc cccctacccc tcattcgaac tcataagata 180

.......... tac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 240

181 tcacagtgga tttatcgaac cgcattagta ccagtatcga caaaggacac actttaacaa 240

Sp6_pri .......... ....... M13_reverse_pri .... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

241 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 300

241 taggcgagtg ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt cggaccccac 300

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

301 cctaatgagt gagctaacca cattaatttg cgttgcgctc actgcccgct ttccagtcgg 360

301 ggattactca ctcgattggt gtaattaaac gcaacgcgag tgacgggcga aaggtcagcc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 420

361 ctttggacag cacggtcgac gtaattactt agccggttgc gcgcccctct ccgccaaacg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 480

421 cataacccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 540

481 ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 acgcaggaaa gaacatgaat taattctcat gtttgacagc ttatcatcgg ataacccctt 600

541 tgcgtccttt cttgtactta attaagagta caaactgtcg aatagtagcc tattggggaa 600

.......... .......... .......... .......... .......... T7_term

.......... .......... .......... .......... .......... ..........

601 ggggcctcta aacgggtctt gaggggtttt ttgttatcat gtttgacagc ttatcttcga 660

601 ccccggagat ttgcccagaa ctccccaaaa aacaatagta caaactgtcg aatagaagct 660

.......... T7_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat 720

661 aatcgaaatt acgccatcaa atagtgtcaa tttaacgatt gcgtcagtcc gtggcacata 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat 780

721 ctttagattg ttacgcgagt agcagtagga gccgtggcag tgggacctac gacatccgta 780

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... EcoRV(820) .......... ..........

781 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat 840

781 tccgaaccaa tacggccatg acggcccgga gaacgcccta tagcaggtaa ggctgtcgta 840

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc 900

841 gcggtcagtg ataccgcacg acgatcgcga tatacgcaac tacgttaaag atacgcgtgg 900

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact 960

901 gcaagagcct cgtgacaggc tggcgaaacc ggcggcgggt caggacgagc gaagcgatga 960

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga ttctctacgc 1020

961 acctcggtga tagctgatgc gctagtaccg ctggtgtggg caggacacct aagagatgcg 1020

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc 1080

1021 gcctgcgtag caccggccgt agtggccgcg gtgtccacgc caacgaccgc ggatatagcg 1080

.......... .......... ORF 2 tet .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

1081 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg 1140

1081 gctgtagtgg ctaccccttc tagcccgagc ggtgaagccc gagtactcgc gaacaaagcc 1140

pBRrevBam_pri ...... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttacatgc 1200

1141 gcacccatac caccgtccgg ggcaccggcc ccctgacaac ccgcggtaga ggaatgtacg 1200

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat 1260

1201 tggtaaggaa cgccgccgcc acgagttgcc ggagttggat gatgacccga cgaaggatta 1260

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcaggagtcg cataagggag agcgccgacc gatgcccttg agagccttca acccagtcag 1320

1261 cgtcctcagc gtattccctc tcgcggctgg ctacgggaac tctcggaagt tgggtcagtc 1320

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat 1380

1321 gaggaaggcc acccgcgccc cgtactgata gcagcggcgt gaatactgac agaagaaata 1380

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt 1440

1381 gtacgttgag catcctgtcc acggccgtcg cgagacccag taaaagccgc tcctggcgaa 1440

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct 1500

1441 agcgacctcg cgctgctact agccggacag cgaacgccat aagccttaga acgtgcggga 1500

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat 1560

1501 gcgagttcgg aagcagtgac cagggcggtg gtttgcaaag ccgctcttcg tccggtaata 1560

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... NruI(1607) ..........

1561 cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg 1620

1561 gcggccgtac cgccggctgc gcgacccgat gcagaacgac cgcaagcgct gcgctccgac 1620

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca 1680

1621 ctaccggaag gggtaatact aagaagagcg aaggccgccg tagccctacg ggcgcaacgt 1680

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc 1740

1681 ccggtacgac aggtccgtcc atctactgct ggtagtccct gtcgaagttc ctagcgagcg 1740

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggctcttacc agcctaactt cgatcactgg accgctgatc gtcacggcga tttatgccgc 1800

1741 ccgagaatgg tcggattgaa gctagtgacc tggcgactag cagtgccgct aaatacggcg 1800

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg 1860

1801 gagccgctcg tgtaccttgc ccaaccgtac ctaacatccg cggcgggata tggaacagac 1860

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg 1920

1861 ggaggggcgc aacgcagcgc cacgtacctc ggcccggtgg agctggactt accttcggcc 1920

.......... .......... ORF 2 tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga 1980

1921 gccgtggagc gattgcctaa gtggtgaggt tcttaacctc ggttagttaa gaacgcctct 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc 2040

1981 tgacacttac gcgtttggtt gggaaccgtc ttgtataggt agcgcaggcg gtagaggtcg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg 2100

2041 tcggcgtgcg ccgcgtagag cccgtcgcaa cccaggaccg gtgcccacgc gtactagcac 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa 2160

2101 gaggacagca actcctgggc cgatccgacc gccccaacgg aatgaccaat cgtcttactt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca 2220

2161 agtggctatg cgctcgcttg cacttcgctg acgacgacgt tttgcagacg ctggactcgt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcccct 2280

2221 tgttgtactt accagaagcc aaaggcacaa agcatttcag acctttgcgc cttcagggga 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acgtgctgct gaagttgccc gcaacagaga gtggaaccaa ccggtgatac cacgatacta 2340

2281 tgcacgacga cttcaacggg cgttgtctct caccttggtt ggccactatg gtgctatgat 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgactgagag tcaacgccat gagcggcctc atttcttatt ctgagttaca acagtccgca 2400

2341 actgactctc agttgcggta ctcgccggag taaagaataa gactcaatgt tgtcaggcgt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ccgctgtccg gtagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg 2460

2401 ggcgacaggc catcgaggaa ggccacccgc gccccgtact gatagcagcg gcgtgaatac 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgcccaa cagtcccccg 2520

2461 tgacagaaga aatagtacgt tgagcatcct gtccacggcc gtcgcgggtt gtcagggggc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gccacggggc ctgccaccat acccacgccg aaacaagcgc cctgcaccat tatgttccgg 2580

2521 cggtgccccg gacggtggta tgggtgcggc tttgttcgcg ggacgtggta atacaaggcc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag 2640

2581 tagacgtagc gtcctacgac gaccgatggg acaccttgtg gatgtagaca taattgcttc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... BclI(2679) .......... ..........

2641 cgctaaccgt ttttatcagg ctctgggagg cagaataaat gatcatatcg tcaattatta 2700

2641 gcgattggca aaaatagtcc gagaccctcc gtcttattta ctagtatagc agttaataat 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cctccacggg gagagcctga gcaaactggc ctcaggcatt tgagaagcac acggtcacac 2760

2701 ggaggtgccc ctctcggact cgtttgaccg gagtccgtaa actcttcgtg tgccagtgtg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac 2820

2761 acgaaggcca tcagttattt ggccatttgg tcgttatctg tattcgccga taaattgctg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt 2880

2821 ggacgggact tggctgctgg cccagcttaa acgaaagctt aaagacggta agtaggcgaa 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact gccttaaaaa 2940

2881 taatagtgaa taagtccgca tcgtggtccg caaattcccg tggttattga cggaattttt 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg 3000

2941 ttaatgcggg gcgggacggt gagtagcgtc atgacaacat taagtaattc gtaagacggc 3000

.......... .......... .......... CAT/CamR .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg 3060

3001 tgtaccttcg gtagtgtctg ccgtactact tggacttagc ggtcgccgta gtcgtggaac 3060

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgccttgcg tataatattt gcccatcgtg aaaacggggg cgaagaagtt gtccatattg 3120

3061 agcggaacgc atattataaa cgggtagcac ttttgccccc gcttcttcaa caggtataac 3120

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata 3180

3121 cggtgcaaat ttagttttga ccactttgag tgggtcccta accgactctg ctttttgtat 3180

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc 3240

3181 aagagttatt tgggaaatcc ctttatccgg tccaaaagtg gcattgtgcg gtgtagaacg 3240

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac 3300

3241 cttatataca catctttgac ggcctttagc agcaccataa gtgaggtctc gctacttttg 3300

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc 3360

3301 caaagtcaaa cgagtacctt ttgccacatt gttcccactt gtgatagggt atagtggtcg 3360

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcaccgtctt tcattgccat acggaaccgg atgagcattc atcaggcggg caagaatgtg 3420

3361 agtggcagaa agtaacggta tgccttggcc tactcgtaag tagtccgccc gttcttacac 3420

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat 3480

3421 ttatttccgg cctattttga acacgaataa aaagaaatgc cagaaatttt tccggcatta 3480

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg 3540

3481 taggtcgact tgccagacca atatccatgt aactcgttga ctgactttac ggagttttac 3540

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat 3600

3541 aagaaatgct acggtaaccc tatatagttg ccaccatata ggtcactaaa aaaagaggta 3600

.......... .......... CAT/CamR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct 3660

3601 aaatcgaagg aatcgaggac ttttagagct attgagtttt ttatgcgggc catcactaga 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca 3720

3661 ataaagtaat accactttca accttggaga atgcacggct agttgcagag taaaagcggt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa 3780

3721 tttcaaccgg gtcccgaagg gccatagttg tccctgtggt cctaaataaa taagacgctt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt 3840

3781 cactagaagg cagtgtccat aaataagccg cgtttcacgc agcccactac gacggttgaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct 3900

3841 tgactaaatc acatactacc acaaaaactc cacgaggtca ccgaagacaa agatagtcga 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc 3960

3901 cagggaggac aagtcgatga ctgccccacc acgcattgcc gttttcgtgg cggcctgtag 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt 4020

3961 tcgcgatcgc ctcacatatg accgaatgat acaaccgtga ctactcccac agtcacttca 4020

.......... .......... ......... p15A_origin ......... ..........

.......... .......... .......... .......... .......... ..........

4021 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg 4080

4021 cgaagtacac cgtcctcttt tttccgacgt ggccacgcag tcgtcttata cactatgtcc 4080

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg 4140

4081 tatataaggc gaaggagcga gtgactgagc gatgcgagcc agcaagctga cgccgctcgc 4140

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg 4200

4141 ctttaccgaa tgcttgcccc gcctctaaag gaccttctac ggtccttcta tgaattgtcc 4200

.......... ......... p15A_origin ......... .......... ..........

......... SacII(4216) ......... .......... .......... ..........

4201 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc 4260

4201 cttcactctc ccggcgccgt ttcggcaaaa aggtatccga ggcgggggga ctgttcgtag 4260

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg 4320

4261 tgctttagac tgcgagttta gtcaccaccg ctttgggctg tcctgatatt tctatggtcc 4320

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt 4380

4321 gcaaagggga ccgccgaggg agcacgcgag aggacaagga cggaaagcca aatggccaca 4380

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg 4440

4381 gtaaggcgac aataccggcg caaacagagt aaggtgcgga ctgtgagtca aggcccatcc 4440

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct 4500

4441 gtcaagcgag gttcgacctg acatacgtgc ttggggggca agtcaggctg gcgacgcgga 4500

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag 4560

4501 ataggccatt gatagcagaa ctcaggttgg gcctttctgt acgttttcgt ggtgaccgtc 4560

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa 4620

4561 gtcggtgacc attaactaaa tctcctcaat cagaacttca gtacgcggcc aattccgatt 4620

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag 4680

4621 tgactttcct gttcaaaacc actgacgcga ggaggttcgg tcaatggagc caagtttctc 4680

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag 4740

4681 aaccatcgag tctcttggaa gctttttggc gggacgttcc gccaaaaaag caaaagtctc 4740

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa 4800

4741 gttctctaat gcgcgtctgg ttttgctaga gttcttctag tagaataatt agtctatttt 4800

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata 4860

4801 ataaagatct aaagtcacgt taaatagaga agtttacatc gtggacttca gtcggggtat 4860

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgatataagt tgtaattctc atgtttgaca gcttatcatc taagaaacca ttattatcat 4920

4861 gctatattca acattaagag tacaaactgt cgaatagtag attctttggt aataatagta 4920

p15A_origin ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 4980

4921 ctgtaattgg atatttttat ccgcatagtg ctccgggaaa gcagagcgcg caaagccact 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 5040

4981 actgccactt ttggagactg tgtacgtcga gggcctctgc cagtgtcgaa cagacattcg 5040

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 5100

5041 cctacggccc tcgtctgttc gggcagtccc gcgcagtcgc ccacaaccgc ccacagcccc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... ......... ApaLI(5140) ......... ..........

5101 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcacccta tgcggtgtga 5160

5101 gaccgaattg atacgccgta gtctcgtcta acatgactct cacgtgggat acgccacact 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgaaattgta aacgttaata 5220

5161 ttatggcgtg tctacgcatt cctcttttat ggcgtagtcc gctttaacat ttgcaattat 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttttgttaaa attcgcgtta aatatttgtt aaatcagctc attttttaac caataggccg 5280

5221 aaaacaattt taagcgcaat ttataaacaa tttagtcgag taaaaaattg gttatccggc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 5340

5281 tttagccgtt ttagggaata tttagttttc ttatctggct ctatcccaac tcacaacaag 5340

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

5341 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 5400

5341 gtcaaacctt gttctcaggt gataatttct tgcacctgag gttgcagttt cccgcttttt 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttgcggt 5460

5401 ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg gtttagttca aaaaacgcca 5460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgaggtgccg taaagctcta aatcggaacc ctaaagggag cccccgattt agagcttgac 5520

5461 gctccacggc atttcgagat ttagccttgg gatttccctc gggggctaaa tctcgaactg 5520

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 5580

5521 cccctttcgg ccgcttgcac cgctctttcc ttcccttctt tcgctttcct cgcccgcgat 5580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 5640

5581 cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg gtgtgggcgg cgcgaattac 5640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc 5700

5641 gcggcgatgt cccgcgcagg taagcggtaa gtccgacgcg ttgacaaccc ttcccgctag 5700

.......... .......... .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

5701 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 5760

5701 ccacgcccgg agaagcgata atgcggtcga ccgctttccc cctacacgac gttccgctaa 5760

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 5820

5761 ttcaacccat tgcggtccca aaagggtcag tgctgcaaca ttttgctgcc ggtcacttaa 5820

.......... ... lacZ_a M13_pUC_fwd_pri M13_forward20_pri ...

.......... .......... .......... .......... .......... ..........

5821 gtaatacgac tcactata.. .......... .......... .......... .......... 5838

5821 cattatgctg agtgatat.. .......... .......... .......... .......... 5838

.......... .......... .......... .......... .......... ..........