Sequence of pALTER-MAX

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Sequence of pALTER-MAX

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Sequence of pALTER-MAX : 5534 BP; 1420 A; 1307 C; 1316 G; 1491 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60

1 agttataacc ggtaatcggt ataataagta accaatatat cgtatttagt tataaccgat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120

61 aaccggtaac gtatgcaaca tagatatagt attatacatg taaatataac cgagtacagg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... SpeI(152) .......... ..........

121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180

121 ttatactggc ggtacaaccg taactaataa ctgatcaata attatcatta gttaatgccc 180

.......... .......... .......... .......... ....... CMV_immearly_pro

.......... .......... .......... .......... .......... ..........

181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg ta aatggccc 240

181 cagtaatcaa gtatcgggta tatacctcaa ggcgcaatgt attgaatgcc atttaccggg 240

.......... ....... CMV_immearly_pro ....... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300

241 cggaccgact ggcgggttgc tgggggcggg taactgcagt tattactgca tacaagggta 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360

301 tcattgcggt tatccctgaa aggtaactgc agttacccac ctcataaatg ccatttgacg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... NdeI(387) .......... .......... ..........

361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420

361 ggtgaaccgt catgtagttc acatagtata cggttcaggc gggggataac tgcagttact 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480

421 gccatttacc gggcggaccg taatacgggt catgtactgg aatgccctga aaggatgaac 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540

481 cgtcatgtag atgcataatc agtagcgata atggtaccac tacgccaaaa ccgtcatgtg 540

....... CAG_enh CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600

541 gttacccgca cctatcgcca aactgagtgc ccctaaaggt tcagaggtgg ggtaactgca 600

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660

601 gttaccctca aacaaaaccg tggttttagt tgccctgaaa ggttttacag cattgttgac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720

661 gctagcgggc ggggcaactg cgtttacccg ccatccgcac atgccaccct ccagatatat 720

.......... . CMV_immearly_pro CMV_fwd_pri CMV_pro . ..........

.......... .......... .......... .......... .......... ..........

721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780

721 tcgtctcgag caaatcactt ggcagtctag tgatcttcga aataacgcca tcaaatagtg 780

CMV_pro pCEP_fwd_pri LNCX_pri .......... .......... ..........

.......... .......... .......... .......... .......... PstI(838)

781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt 840

781 tcaatttaac gattgcgtca gtcacgaaga ctgtgttgtc agagcttgaa ttcgacgtca 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa 900

841 ctgagagaat tccatcggaa cgtcttcaac cagcactccg tgacccgtcc attcatagtt 900

.......... .......... .......... .......... .......... chim_int

.......... .......... .......... .......... .......... ..........

901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact 960

901 ccaatgttct gtccaaattc ctctggttat ctttgacccg aacagctctg tctcttctga 960

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac 1020

961 gaacgcaaag actatccgtg gataaccaga atgactgtag gtgaaacgga aagagaggtg 1020

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact 1080

1021 tccacaggtg agggtcaagt taatgtcgag aattccgatc tcatgaatta tgctgagtga 1080

.......... .......... .......... .......... .......... T7_pro

XhoI,EcoRI KpnI,XbaI SalI SmaI,NotI31),EagI(1131)

1081 ataggctagc ctcgagaatt cacgcgtggt acctctagag tcgacccggg cggccgcaaa 1140

1081 tatccgatcg gagctcttaa gtgcgcacca tggagatctc agctgggccc gccggcgttt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gttcccttta gtgagggtta atgcttcgag cagacatgat aagatacatt gatgagtttg 1200

1141 caagggaaat cactcccaat tacgaagctc gtctgtacta ttctatgtaa ctactcaaac 1200

.......... .......... .......... ........ SV40_PA_term EBV_rev_pri

.......... .......... .......... .......... .......... ..........

1201 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 1260

1201 ctgtttggtg ttgatcttac gtcacttttt ttacgaaata aacactttaa acactacgat 1260

EBV_rev_pri ....... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... HpaI(1301) ..........

1261 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 1320

1261 aacgaaataa acattggtaa tattcgacgt tatttgttca attgttgttg ttaacgtaag 1320

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct 1380

1321 taaaatacaa agtccaagtc cccctctaca ccctccaaaa aatttcgttc attttggaga 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acaaatgtgg taaaatccga taaggatcga tccgggctgg cgtaatagcg aagaggcccg 1440

1381 tgtttacacc attttaggct attcctagct aggcccgacc gcattatcgc ttctccgggc 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc 1500

1441 gtggctagcg ggaagggttg tcaacgcgtc ggacttaccg cttacctgcg cgggacatcg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 1560

1501 ccgcgtaatt cgcgccgccc acaccaccaa tgcgcgtcgc actggcgatg tgaacggtcg 1560

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gccctagcgc ccgctccttt cgctttcttc ccttcctttc acgccacgtt cgccggcttt 1620

1561 cgggatcgcg ggcgaggaaa gcgaaagaag ggaaggaaag tgcggtgcaa gcggccgaaa 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 1680

1621 ggggcagttc gagatttagc ccccgaggga aatcccaagg ctaaatcacg aaatgccgtg 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 1740

1681 gagctggggt tttttgaact aatcccacta ccaagtgcat cacccggtag cgggactatc 1740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 1800

1741 tgccaaaaag cgggaaactg caacctcagg tgcaagaaat tatcacctga gaacaaggtt 1800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 1860

1801 tgaccttgtt gtgagttggg atagagccag ataagaaaac taaatattcc ctaaaacggc 1860

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 1920

1861 taaagccgga taaccaattt tttactcgac taaattgttt ttaaattgcg cttaaaattg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta 1980

1921 ttttataatt gcgaatgtta aaggactacg ccataaaaga ggaatgcgta gacacgccat 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tttcacaccg catacgcgga tcttccgtac cttctgaggc ggaaagaacc agctgtggaa 2040

1981 aaagtgtggc gtatgcgcct agaaggcatg gaagactccg cctttcttgg tcgacacctt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 2100

2041 acacacagtc aatcccacac ctttcagggg tccgaggggt cgtccgtctt catacgtttc 2100

pBABE_3_pri ........ ...... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

2101 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 2160

2101 gtacgtagag ttaatcagtc gttggtccac acctttcagg ggtccgaggg gtcgtccgtc 2160

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 2220

2161 ttcatacgtt tcgtacgtag agttaatcag tcgttggtat cagggcgggg attgaggcgg 2220

.......... ...... SV40_enh SV40_pro SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

2221 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 2280

2221 gtagggcggg gattgaggcg ggtcaaggcg ggtaagaggc ggggtaccga ctgattaaaa 2280

..... SV40_enh SV40_pro SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2281 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 2340

2281 aaaataaata cgtctccggc tccggcggag ccggagactc gataaggtct tcatcactcc 2340

SV40_origin SV40pro_F_pri SV40_pro SV40_pro ...... ..........

.......... StuI(2355) .......... .......... .......... ..........

2341 aggctttttt ggaggcctag gcttttgcaa aaagcttgat tcttctgaca caacagtctc 2400

2341 tccgaaaaaa cctccggatc cgaaaacgtt tttcgaacta agaagactgt gttgtcagag 2400

.......... SV40_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaacttaagg ctagcgaata aatacctgtg acggaagatc acttcgcaga ataaataaat 2460

2401 cttgaattcc gatcgcttat ttatggacac tgccttctag tgaagcgtct tatttattta 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa gtgagacgtt 2520

2461 ggaccacagg gacaactatg gcccttcggg acccggttga aaaccgcttt cactctgcaa 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gatcggcacg taagaggttc caactttcac cataatcaaa taagatcact accgggcgta 2580

2521 ctagccgtgc attctccaag gttgaaagtg gtattagttt attctagtga tggcccgcat 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact 2640

2581 aaaaaactca atagctctaa aagtcctcga ttccttcgat tttacctctt tttttagtga 2640

.......... .......... .......... .......... ........ ORF 1 CAT/CamR

.......... .......... .......... .......... .......... ..........

2641 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag 2700

2641 cctatatggt ggcaactata tagggttacc gtagcatttc ttgtaaaact ccgtaaagtc 2700

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag 2760

2701 agtcaacgag ttacatggat attggtctgg caagtcgacc tataatgccg gaaaaatttc 2760

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg 2820

2761 tggcatttct ttttattcgt gttcaaaata ggccggaaat aagtgtaaga acgggcggac 2820

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atgaatgctc atccggaact ccgtatggca atgaaagacg gtgagctggt gatatgggat 2880

2821 tacttacgag taggccttga ggcataccgt tactttctgc cactcgacca ctatacccta 2880

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg 2940

2881 tcacaagtgg gaacaatgtg gcaaaaggta ctcgtttgac tttgcaaaag tagcgagacc 2940

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt 3000

2941 tcacttatgg tgctgctaaa ggccgtcaaa gatgtgtata taagcgttct acaccgcaca 3000

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca 3060

3001 atgccacttt tggaccggat aaagggattt cccaaataac tcttatacaa aaagcagagt 3060

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc 3120

3061 cggttaggga cccactcaaa gtggtcaaaa ctaaatttgc accggttata cctgttgaag 3120

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg 3180

3121 aagcgggggc aaaagtggta cccgtttata atatgcgttc cgctgttcca cgactacggc 3180

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat 3240

3181 gaccgctaag tccaagtagt acggcaaaca ctaccgaagg tacagccgtc ttacgaatta 3240

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat 3300

3241 cttaatgttg tcatgacgct actcaccgtc ccgccccgca ttaaaaaaat tccgtcaata 3300

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc 3360

3301 accacgggaa tttgcggacc aacgatgcgg acttattcac tattattcgc ctacttaccg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agaaattcgt cgaagcgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt 3420

3361 tctttaagca gcttcgcgtt attttataga aataaaagta atgtagacac acaaccaaaa 3420

.......... .......... ........ synth_PA_term ........ ..........

.......... .......... .......... .......... .......... ..........

3421 ttgtgtgaat cgatagcgat aaggatcgga agatccgcgt atggtgcact ctcagtacaa 3480

3421 aacacactta gctatcgcta ttcctagcct tctaggcgca taccacgtga gagtcatgtt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 3540

3481 agacgagact acggcgtatc aattcggtcg gggctgtggg cggttgtggg cgactgcgcg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 3600

3541 ggactgcccg aacagacgag ggccgtaggc gaatgtctgt tcgacactgg cagaggccct 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 3660

3601 cgacgtacac agtctccaaa agtggcagta gtggctttgc gcgctctgct ttcccggagc 3660

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 3720

3661 actatgcgga taaaaatatc caattacagt actattatta ccaaagaatc tgcagtccac 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 3780

3721 cgtgaaaagc ccctttacac gcgccttggg gataaacaaa taaaaagatt tatgtaagtt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 3840

3781 tatacatagg cgagtactct gttattggga ctatttacga agttattata actttttcct 3840

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 3900

3841 tctcatactc ataagttgta aaggcacagc gggaataagg gaaaaaacgc cgtaaaacgg 3900

.......... .......... .......... Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 3960

3901 aaggacaaaa acgagtgggt ctttgcgacc actttcattt tctacgactt ctagtcaacc 3960

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 4020

3961 cacgtgctca cccaatgtag cttgacctag agttgtcgcc attctaggaa ctctcaaaag 4020

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 4080

4021 cggggcttct tgcaaaaggt tactactcgt gaaaatttca agacgataca ccgcgccata 4080

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 4140

4081 atagggcaca actgcggccc gttctcgttg agccagcggc gtatgtgata agagtcttac 4140

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 4200

4141 tgaaccaact catgagtggt cagtgtcttt tcgtagaatg cctaccgtac tgtcattctc 4200

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 4260

4201 ttaatacgtc acgacggtat tggtactcac tattgtgacg ccggttgaat gaagactgtt 4260

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 4320

4261 gctagcctcc tggcttcctc gattggcgaa aaaacgtgtt gtacccccta gtacattgag 4320

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 4380

4321 cggaactagc aacccttggc ctcgacttac ttcggtatgg tttgctgctc gcactgtggt 4380

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgatgccgca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 4440

4381 gctacggcgt taccgttgtt gcaacgcgtt tgataattga ccgcttgatg aatgagatcg 4440

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 4500

4441 aagggccgtt gttaattatc tgacctacct ccgcctattt caacgtcctg gtgaagacgc 4500

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 4560

4501 gagccgggaa ggccgaccga ccaaataacg actatttaga cctcggccac tcgcacccag 4560

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 4620

4561 agcgccatag taacgtcgtg accccggtct accattcggg agggcatagc atcaatagat 4620

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 4680

4621 gtgctgcccc tcagtccgtt gatacctact tgctttatct gtctagcgac tctatccacg 4680

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 4740

4681 gagtgactaa ttcgtaacca ttgacagtct ggttcaaatg agtatatatg aaatctaact 4740

.......... Ampicillin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 4800

4741 aaattttgaa gtaaaaatta aattttccta gatccacttc taggaaaaac tattagagta 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 4860

4801 ctggttttag ggaattgcac tcaaaagcaa ggtgactcgc agtctggggc atcttttcta 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 4920

4861 gtttcctaga agaactctag gaaaaaaaga cgcgcattag acgacgaacg tttgtttttt 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 4980

4921 tggtggcgat ggtcgccacc aaacaaacgg cctagttctc gatggttgag aaaaaggctt 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 5040

4981 ccattgaccg aagtcgtctc gcgtctatgg tttatgacaa gaagatcaca tcggcatcaa 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 5100

5041 tccggtggtg aagttcttga gacatcgtgg cggatgtatg gagcgagacg attaggacaa 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 5160

5101 tggtcaccga cgacggtcac cgctattcag cacagaatgg cccaacctga gttctgctat 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 5220

5161 caatggccta ttccgcgtcg ccagcccgac ttgcccccca agcacgtgtg tcgggtcgaa 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 5280

5221 cctcgcttgc tggatgtggc ttgactctat ggatgtcgca ctcgatactc tttcgcggtg 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 5340

5281 cgaagggctt ccctctttcc gcctgtccat aggccattcg ccgtcccagc cttgtcctct 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 5400

5341 cgcgtgctcc ctcgaaggtc cccctttgcg gaccatagaa atatcaggac agcccaaagc 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 5460

5401 ggtggagact gaactcgcag ctaaaaacac tacgagcagt ccccccgcct cggatacctt 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 5520

5461 tttgcggtcg ttgcgccgga aaaatgccaa ggaccggaaa acgaccggaa aacgagtgta 5520

pBR322_origin ...... .......... .......... .......... ..........

BglII(5529) ........ .......... .......... .......... ..........

5521 ggctcgacag atct...... .......... .......... .......... .......... 5534

5521 ccgagctgtc taga...... .......... .......... .......... .......... 5534

.......... .......... .......... .......... .......... ..........