ID PAMP1 preliminary; circular DNA; SYN; 4114 BP. XX AC IG1310; XX DT 01-OCT-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli phagemid vector pAMP1 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC [pAMP1, pAMP2, pAMP10 from pSPORT1 & oligo] RC [pAMP18 from pUC18 & oligo] RC [pAMP19 from pUC19 & oligo] RA Eckert K.A., Kunkle T.A.; RT "DNA polymerase fidelity and the polymerase chain reaction"; RL PCR Meth. Appl. 1:17-24(1991). XX CC Created by Moore, July 1995, under contract with NCBI. CC NM (pAMP1) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (phagemid) CC SP (BRL) CC HO (E.coli) CC CP (high) CC FN (cloning)(expression)(transcription) CC SE () CC PA (pSPORT1) CC BR () CC OF () CC OR () XX FH Key Location/Qualifiers FH FT misc_feature 0..0 FT /note="1. pSPORT1 remove NotI-SalI 21bp 233..254, FT \ MCS 4088bp FT 2. oligo NotI-XmaIII-SalI 30bp FT \ ggccgcctactactactaatgatgatgatg FT -> pAMP1 4118bp" FT - 1..232 FT /note="pSPORT1 1..232 232bp FT NotI = GC^GGCCGC FT \ ggccgcctactactactaatgatgatgatg" FT - 233..262 FT /note="ggccgcctactactactaatgatgatgatg 30bp FT \ ggccgcctactactactaatgatgatgatg FT SalI = G^TCGA C" FT - 263..4114 FT /note="pSPORT1 258..4109 3852bp" FT CDS complement(0..0) FT /note="REP E. coli lacZ gene" FT promoter 0..0 FT /note="PRO bacteriophage Sp6" FT misc_binding 125..381 FT /note="MCS AatII-SphI-MluI-SunI-SnaBI-HindIII-BamHI- FT XbaI-NotI-XmaIII-SalI/AccI-SmaI-EcoRI-Kpn2I-RsrII- FT PinAI-KpnI-Sse8387I-PstI" FT promoter complement(0..0) FT /note="PRO bacteriophage T7" FT CDS complement(0..0) FT /note="REP E. coli lacI repressor gene" FT rep_origin complement(0..0) FT /note="ORI E. coli pMB1 (ColE1 and pBR322)" FT CDS complement(0..0) FT /note="ANT E. coli beta-lactamase gene (bla) FT ampicillin resistance gene (apr/amp)" FT rep_origin 0..0 FT /note="ORI bacteriophage f1" XX SQ Sequence 4114 BP; 1006 A; 1063 C; 1060 G; 985 T; 0 other; cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gcggccgcct actactacta atgatgatga tgcccgggaa ttccggaccg gtacctgcag gcgtaccagc tttccctata gtgagtcgta ttagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttgacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacccg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtc // pAMP1 "Times New RomanC lacZ_a"Times New RomanC}M13_pUC_fwd_pri"Times New RomanCɆM13_forward20_pri"Times New Roman*Sp6_pri"Times New RomanC1CT7_pro"Times New RomanCRdM13_reverse_pri"Times New RomanCɆcyM13_pUC_rev_pri"Times New Romanlac_pro"Times New RomanCORF 1"Times New RomanClacI"Times New RomanCɆd  pBR322_origin"Times New Romanj ORF 2"Times New Roman(Cj  Ampicillin"Times New RomanC AmpR_pro"Times New RomanCɆ f1_origin"Times New Roman sAatII(195),HindIII(213),BamHI(219),XbaI(225),NotI(232),EagI(232),SmaI(265),EcoRI(268),AgeI(277),KpnI(284),PstI(289)"Times New Roman  *T NarI"Times New Roman.U:d,rr EcoRV"Times New Roman{rjaZh ApaI"Times New Roman BclI"Times New Roman   test"Times New Roman((5u5qP test"Times New Roman}(i