Sequence of pAMP1

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Sequence of pAMP1

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Sequence of pAMP1 : 4114 BP; 1006 A; 1063 C; 1060 G; 985 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 60

1 gtaagcggta agtccgacgc gttgacaacc cttcccgcta gccacgcccg gagaagcgat 60

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg 120

61 aatgcggtcg accgctttcc ccctacacga cgttccgcta attcaaccca ttgcggtccc 120

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata 180

121 aaaagggtca gtgctgcaac attttgctgc cggtcactta acttaaatcc actgtgatat 180

lacZ_a M13_pUC_fwd_pri M13_forward20_pri .......... Sp6_pri

.......... AatII(195) ... HindIII(213) BamHI(219) XbaI(225) ...

181 gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gcggccgcct 240

181 cttctcgata ctgcagcgta cgtgcgcatg cattcgaacc taggagatct cgccggcgga 240

.......... .......... .......... .......... .......... ..........

.......... SmaI265),EcoRI(268) AgeI(277) KpnI(284),PstI(289)

241 actactacta atgatgatga tgcccgggaa ttccggaccg gtacctgcag gcgtaccagc 300

241 tgatgatgat tactactact acgggccctt aaggcctggc catggacgtc cgcatggtcg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tttccctata gtgagtcgta ttagagcttg gcgtaatcat ggtcatagct gtttcctgtg 360

301 aaagggatat cactcagcat aatctcgaac cgcattagta ccagtatcga caaaggacac 360

.......... T7_pro .......... ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

361 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 420

361 actttaacaa taggcgagtg ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt 420

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

421 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 480

421 cggaccccac ggattactca ctcgattgag tgtaattaac gcaacgcgag tgacgggcga 480

.......... .......... .......... .......... .......... ORF 1 lacI

.......... .......... .......... .......... .......... ..........

481 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 540

481 aaggtcagcc ctttggacag cacggtcgac gtaattactt agccggttgc gcgcccctct 540

.......... .......... ORF 1 lacI .......... .......... ..........

.......... NarI(558) .......... .......... .......... ..........

541 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag 600

541 ccgccaaacg cataacccgc ggtcccacca aaaagaaaag tggtcactct gcccgttgtc 600

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg 660

601 gactaacggg aagtggcgga ccgggactct ctcaacgtcg ttcgccaggt gcgaccaaac 660

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ccccagcagg cgaaaatcct gtttgatggt ggttgacggc gggatataac atgagctgtc 720

661 ggggtcgtcc gcttttagga caaactacca ccaactgccg ccctatattg tactcgacag 720

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... EcoRV(750) .......... .......... ..........

721 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt 780

721 aagccatagc agcatagggt gatggctcta taggcgtggt tgcgcgtcgg gcctgagcca 780

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac 840

781 ttaccgcgcg taacgcgggt cgcggtagac tagcaaccgt tggtcgtagc gtcacccttg 840

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc 900

841 ctacgggagt aagtcgtaaa cgtaccaaac aacttttggc ctgtaccgtg aggtcagcgg 900

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag 960

901 aagggcaagg cgatagccga cttaaactaa cgctcactct ataaatacgg tcggtcggtc 960

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... ApaI(993) .......... ..........

961 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc 1020

961 tgcgtctgcg cggctctgtc ttgaattacc cgggcgattg tcgcgctaaa cgaccactgg 1020

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact 1080

1021 gttacgctgg tctacgaggt gcgggtcagc gcatggcaga agtaccctct tttattatga 1080

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc 1140

1081 caactaccca cagaccagtc tctgtagttc tttattgcgg ccttgtaatc acgtccgtcg 1140

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... BclI(1182) ..........

1141 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacccg 1200

1141 aaggtgtcgt taccgtagga ccagtaggtc gcctatcaat tactagtcgg gtgactgggc 1200

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat 1260

1201 aacgcgctct tctaacacgt ggcggcgaaa tgtccgaagc tgcggcgaag caagatggta 1260

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg 1320

1261 gctgtggtgg tgcgaccgtg ggtcaactag ccgcgctcta aattagcggc gctgttaaac 1320

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc 1380

1321 gctgccgcgc acgtcccggt ctgacctcca ccgttgcggt tagtcgttgc tgacaaacgg 1380

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac 1440

1381 gcggtcaaca acacggtgcg ccaaccctta cattaagtcg aggcggtagc ggcgaaggtg 1440

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg 1500

1441 aaaaagggcg caaaagcgtc tttgcaccga ccggaccaag tggtgcgccc tttgccagac 1500

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac 1560

1501 tattctctgt ggccgtatga gacgctgtag catattgcaa tgaccaaagt gtaagtggtg 1560

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc 1620

1561 ggacttaact gagagaaggc ccgcgatagt acggtatggc gctttccaaa acgcggtaag 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct ctgcattaat 1680

1621 ctaccacagt tgcatttacg gcgaagcgga agcgcgcgct taacgttcga gacgtaatta 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 1740

1681 cttagccggt tgcgcgcccc tctccgccaa acgcataacc cgcgagaagg cgaaggagcg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 1800

1741 agtgactgag cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 1860

1801 gccattatgc caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 1920

1861 cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg 1920

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1921 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 1980

1921 cggggggact gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2040

1981 ctgatatttc tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2100

2041 gggacggcga atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2160

2101 ttacgagtgc gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 2220

2161 acgtgcttgg ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 2280

2221 ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 2340

2281 ctcgctccat acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 2400

2341 gatcttcctg tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 2460

2401 aaccatcgag aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 2520

2461 tcgtcgtcta atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 2580

2521 ccagactgcg agtcaccttg cttttgagtg caattcccta aaaccagtac tctaatagtt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 2640

2581 tttcctagaa gtggatctag gaaaatttaa tttttacttc aaaatttagt tagatttcat 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 2700

2641 atatactcat ttgaaccaga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc 2700

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2701 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 2760

2701 gctagacaga taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 2820

2761 atgccctccc gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 2880

2821 gccgaggtct aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 2940

2881 gacgttgaaa taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 3000

2941 caagcggtca attatcaaac gcgttgcaac aacggtaacg atgtccgtag caccacagtg 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3060

3001 cgagcagcaa accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3120

3061 ctagggggta caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3180

3121 cattcaaccg gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 3240

3181 agtacggtag gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 3300

3241 ttatcacata cgccgctggc tcaacgagaa cgggccgcag ttatgcccta ttatggcgcg 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 3360

3301 gtgtatcgtc ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 3420

3361 gttcctagaa tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 3480

3421 gaagtcgtag aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 3540

3481 ggcgtttttt cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 3600

3541 ttataataac ttcgtaaata gtcccaataa cagagtactc gcctatgtat aaacttacat 3600

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

3601 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa 3660

3601 aaatcttttt atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac ggtggacttt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 3720

3661 aacatttgca attataaaac aattttaagc gcaatttaaa aacaatttag tcgagtaaaa 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 3780

3721 aattggttat ccggctttag ccgttttagg gaatatttag ttttcttatc tggctctatc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 3840

3781 ccaactcaca acaaggtcaa accttgttct caggtgataa tttcttgcac ctgaggttgc 3840

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 3900

3841 agtttcccgc tttttggcag atagtcccgc taccgggtga tgcacttggt agtgggatta 3900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 3960

3901 gttcaaaaaa ccccagctcc acggcatttc gtgatttagc cttgggattt ccctcggggg 3960

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 4020

3961 ctaaatctcg aactgcccct ttcggccgct tgcaccgctc tttccttccc ttctttcgct 4020

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 4080

4021 ttcctcgccc gcgatcccgc gaccgttcac atcgccagtg cgacgcgcat tggtggtgtg 4080

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgccgcgct taatgcgccg ctacagggcg cgtc...... .......... .......... 4114

4081 ggcggcgcga attacgcggc gatgtcccgc gcag...... .......... .......... 4114

f1_origin .......... .......... .......... .......... ..........

References

Reference number: [1] Reference comment: [pAMP1, pAMP2, pAMP10 from pSPORT1 & oligo] Reference comment: [pAMP18 from pUC18 & oligo] Reference comment: [pAMP19 from pUC19 & oligo] Reference author: Eckert K.A., Kunkle T.A.; Reference title: "DNA polymerase fidelity and the polymerase chain reaction"; Reference location: PCR Meth. Appl. 1:17-24(1991).