pu<Apu<Aÿ´'ID EU606014; SV 1; circular; other DNA; STD; SYN; 6010 BP. AC EU606014; DT 02-OCT-2008 (Rel. 97, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 2) DE Broad host range vector pAP20, complete sequence. KW . OS Broad host range vector pAP20 OC other sequences; artificial sequences; vectors. RN [1] RP 1-6010 RX DOI; 10.1128/JB.00481-08. RX PUBMED; 18776009. RA Law R.J., Hamlin J.N., Sivro A., McCorrister S.J., Cardama G.A., RA Cardona S.T.; RT "A functional phenylacetic acid catabolic pathway is required for full RT pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans RT host model"; RL J. Bacteriol. 190(21):7209-7218(2008). RN [2] RP 1-6010 RA Law R.J., Hamlin J.N.R., Sivro A., McCorrister S.J., Cardama G., RA Cardona S.T.; RT ; RL Submitted (02-APR-2008) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Manitoba, 45 Chancellors Circle, 418 Buller RL Bldg, Winnipeg, Manitoba R3T 2N2, Canada FH Key Location/Qualifiers FT source 1..6010 FT /organism="Broad host range vector pAP20" FT /mol_type="other DNA" FT /note="derived from B. bronchiseptica plasmid pBBR1 genes FT for mobilization and replication" FT /db_xref="taxon:545165" FT promoter 127..517 FT /note="dihydrofolate reductase promoter" FT misc_feature complement(5109..5982) FT /note="chloramphenicol acetyltransferase cassette" FT gene complement(5115..5753) FT /gene="CAT" FT CDS complement(5115..5753) FT /codon_start=1 FT /transl_table=11 FT /gene="CAT" FT /product="chloramphenicol acetyltransferase" FT /note="chloramphenicol resistance" FT /protein_id="ACF35273.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR" SQ Sequence 6010 BP; 1293 A; 1709 C; 1653 G; 1355 T; 0 other; atcttaggat ctcccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc 60 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta 120 ctcgagtatg ctagcgatga gctcgcacga acccagttga cataagcctg ttcggttcgt 180 aaactgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc 240 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag 300 tctagcctcg ggcatccaag ctagctaagc gcgttacgcc gtgggtcgat gtttgatgtt 360 atggaacagc aacgatgtta cgcagcaggg tagtcgccct aaaacaaagt taggcagccg 420 ttgtgctggt gctttctagt agttgttgtg gggtaggcag tcagagctcg atttgcttgt 480 cgccataata gattcacaag aaggattcgg aattctacat atgtcatcga caccatggta 540 cccggggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag 600 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 660 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 720 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 780 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 840 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 900 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 960 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttcc actcactaca 1020 gcagagccat ttaaacaaca tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc 1080 ccgctacggg ctttttcatg ccctgcccta gcgtccaagc ctcacggccg cgctcggcct 1140 ctctggcggc cttctggcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc 1200 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt 1260 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt 1320 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa 1380 gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc 1440 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc 1500 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt 1560 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca 1620 acctattaat ttcccctcgt caaaaataag gttatcaagc gagaaatcac catgagtgac 1680 gactgaatcc ggtgagaatg gcaaaagcta atgcatagct cctgaaaatc tcgataactc 1740 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg 1800 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac 1860 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cgaagacgaa 1920 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 1980 cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc gctgggcctg 2040 tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg ccttgatgat 2100 cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca gaacgggctt 2160 caggcgctcc cgaaggtctc gggccgtctc ttgggcttga tcggccttct tgcgcatctc 2220 acgcgctcct gcggcggcct gtagggcagg ctcatacccc tgccgaaccg cttttgtcag 2280 ccggtcggcc acggcttccg gcgtctcaac gcgctttgag attcccagct tttcggccaa 2340 tccctgcggt gcataggcgc gtggctcgac cgcttgcggg ctgatggtga cgtggcccac 2400 tggtggccgc tccagggcct cgtagaacgc ctgaatgcgc gtgtgacgtg ccttgctgcc 2460 ctcgatgccc cgttgcagcc ctagatcggc cacagcggcc gcaaacgtgg tctggtcgcg 2520 ggtcatctgc gctttgttgc cgatgaactc cttggccgac agcctgccgt cctgcgtcag 2580 cggcaccacg aacgcggtca tgtgcgggct ggtttcgtca cggtggatgc tggccgtcac 2640 gatgcgatcc gccccgtact tgtccgccag ccacttgtgc gccttctcga agaacgccgc 2700 ctgctgttct tggctggccg acttccacca ttccgggctg gccgtcatga cgtactcgac 2760 cgccaacaca gcgtccttgc gccgcttctc tggcagcaac tcgcgcagtc ggcccatcgc 2820 ttcatcggtg ctgctggccg cccagtgctc gttctctggc gtcctgctgg cgtcagcgtt 2880 gggcgtctcg cgctcgcggt aggcgtgctt gagactggcc gccacgttgc ccattttcgc 2940 cagcttcttg catcgcatga tcgcgtatgc cgccatgcct gcccctccct tttggtgtcc 3000 aaccggctcg acgggggcag cgcaaggcgg tgcctccggc gggccactca atgcttgagt 3060 atactcacta gactttgctt cgcaaagtcg tgaccgccta cggcggctgc ggcgccctac 3120 gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc tcccttgcca gcccgtggat 3180 atgtggacga tggccgcgag cggccaccgg ctggctcgct tcgctcggcc cgtggacaac 3240 cctgctggac aagctgatgg acaggctgcg cctgcccacg agcttgacca cagggattgc 3300 ccaccggcta cccagccttc gaccacatac ccaccggctc caactgcgcg gcctgcggcc 3360 ttgccccatc aattttttta attttctctg gggaaaagcc tccggcctgc ggcctgcgcg 3420 cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca gcgaccgcgc 3480 agcggcttgg ccttgacgcg cctggaacga cccaagccta tgcgagtggg ggcagtcgaa 3540 ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg gttccaaggg 3600 ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc cccggagggg 3660 ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc aggggtgccc 3720 ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa aaatcaacaa 3780 cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag ctcattgcgt 3840 aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt gttgtcgcgc 3900 tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc ctaccgcatg 3960 gagataagca tggccacgca gtccagagaa atcggcattc aagccaagaa caagcccggt 4020 cactgggtgc aaacggaacg caaagcgcat gaggcgtggg ccgggcttat tgcgaggaaa 4080 cccacggcgg caatgctgct gcatcacctc gtggcgcaga tgggccacca gaacgccgtg 4140 gtggtcagcc agaagacact ttccaagctc atcggacgtt ctttgcggac ggtccaatac 4200 gcagtcaagg acttggtggc cgagcgctgg atctccgtcg tgaagctcaa cggccccggc 4260 accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg gccagccccg cgaccagttg 4320 cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg acgaccagga cgaatcgctg 4380 ttggggcatg gcgacctgcg ccgcatcccg accctgtatc cgggcgagca gcaactaccg 4440 accggccccg gcgaggagcc gcccagccag cccggcattc cgggcatgga accagacctg 4500 ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct gccgatgccc 4560 gatgagccgt gttttctgga cgatggcgag ccgttggagc cgccgacacg ggtcacgctg 4620 ccgcgccggt agcacttggg ttgcgcagca acccgtaagt gcgctgttcc agactatcgg 4680 ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 4740 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccgtt 4800 tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg 4860 agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta 4920 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa 4980 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta 5040 ttcaggcgta gcaccaggcg tttaagtcga ccaataactc tacggggtct gacgctcagt 5100 ggaacgaaat cgattcatcg cagtactgtt gtattcatta agcatctgcc gacatggaag 5160 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 5220 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 5280 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 5340 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 5400 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 5460 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 5520 ttcattgcca tacgtaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 5580 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 5640 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 5700 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 5760 tccttagctc ctgaaaatct cgacaactca aaaaatacgc ccggtagtga 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