Sequence of pAP20

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Sequence of pAP20

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Sequence of pAP20: 6010 BP; 1293 A; 1709 C; 1653 G; 1355 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atcttaggat ctcccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc 60

1 tagaatccta gagggtagcc actacagccg ctatatccgc ggtcgttggc gtggacaccg 60

.......... ....... pBRrevBam_primer ....... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta 120

61 cggccactac ggccggtgct acgcaggccg catctcctag acgagtacaa actgtcgaat 120

.......... .......... .......... .......... .......... ..........

XhoI(122) NheI(131) .......... .......... .......... ..........

121 ctcgagtatg ctagcgatga gctcgcacga acccagttga cataagcctg ttcggttcgt 180

121 gagctcatac gatcgctact cgagcgtgct tgggtcaact gtattcggac aagccaagca 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aaactgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc 240

181 tttgacatta cgttcatcgc atacgcgagt gcgttgacca ggtcttggaa ctggcttgcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag 300

241 tcgccaccat tgccgcgtca ccgccaaaag taccgaacaa tactgacaaa aaaacatgtc 300

.......... .......... .......... .......... .......... ..........

.......... .......... NheI(321) .......... .......... ..........

301 tctagcctcg ggcatccaag ctagctaagc gcgttacgcc gtgggtcgat gtttgatgtt 360

301 agatcggagc ccgtaggttc gatcgattcg cgcaatgcgg cacccagcta caaactacaa 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 atggaacagc aacgatgtta cgcagcaggg tagtcgccct aaaacaaagt taggcagccg 420

361 taccttgtcg ttgctacaat gcgtcgtccc atcagcggga ttttgtttca atccgtcggc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttgtgctggt gctttctagt agttgttgtg gggtaggcag tcagagctcg atttgcttgt 480

421 aacacgacca cgaaagatca tcaacaacac cccatccgtc agtctcgagc taaacgaaca 480

.......... .......... .......... .......... .......... ..........

.......... .......... ..... EcoRI(511) NdeI(520) ..... ..........

481 cgccataata gattcacaag aaggattcgg aattctacat atgtcatcga caccatggta 540

481 gcggtattat ctaagtgttc ttcctaagcc ttaagatgta tacagtagct gtggtaccat 540

.......... .......... .......... .......... .......... ..........

XmaI,KpnI,SmaI,BamHI XbaI SalI PstI) HindIII(577)

541 cccggggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag 600

541 gggcccctag gagatctcag ctggacgtcc gtacgttcga accgacaaaa ccgcctactc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 660

601 tcttctaaaa gtcggactat gtctaattta gtcttgcgtc ttcgccagac tattttgtct 660

........ pTrcHis_rev_primer pBAD_rev_primer ........ ..........

.......... .......... .......... .......... .......... ..........

661 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 720

661 taaacggacc gccgtcatcg cgccaccagg gtggactggg gtacggcttg agtcttcact 720

.......... .......... ....... rrnB_terminator ....... ..........

.......... .......... .......... .......... .......... ..........

721 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 780

721 ttgcggcatc gcggctacca tcacacccca gaggggtacg ctctcatccc ttgacggtcc 780

.......... ....... rrnB_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 840

781 gtagtttatt ttgctttccg agtcagcttt ctgacccgga aagcaaaata gacaacaaac 840

.......... ....... rrnB_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 900

841 agccacttgc gagaggactc atcctgttta ggcggccctc gcctaaactt gcaacgcttc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 960

901 gttgccgggc ctcccaccgc ccgtcctgcg ggcggtattt gacggtccgt agtttaattc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttcc actcactaca 1020

961 gtcttccggt aggactgcct accggaaaaa cgcaaagatg tttgagaagg tgagtgatgt 1020

...... rrnB_T2_terminator ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcagagccat ttaaacaaca tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc 1080

1021 cgtctcggta aatttgttgt aggggagggg gaaaggtggc gcagtctgcg ggcatcgtcg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... EagI(1126) ..........

1081 ccgctacggg ctttttcatg ccctgcccta gcgtccaagc ctcacggccg cgctcggcct 1140

1081 ggcgatgccc gaaaaagtac gggacgggat cgcaggttcg gagtgccggc gcgagccgga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctctggcggc cttctggcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc 1200

1141 gagaccgccg gaagaccgcg actccagacg gagcacttct tccacaacga ctgagtatgg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt 1260

1201 tccggactta gcggggtagt aggtcggtct ttcactccct cggtgccaac tactctcgaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt 1320

1261 acaacatcca cctggtcaac cactaaaact tgaaaacgaa acggtgcctt gccagacgca 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa 1380

1321 acagcccttc tacgcactag actaggaagt tgagtcgttt tcaagctaaa taagttgttt 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc 1440

1381 cggcggcagg gcagttcagt cgcattacga gacggtcaca atgttggtta attggttaag 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc 1500

1441 actaatcttt ttgagtagct cgtagtttac tttgacgtta aataagtata gtcctaatag 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt 1560

1501 ttatggtata aaaacttttt cggcaaagac attacttcct cttttgagtg gctccgtcaa 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca 1620

1561 ggtatcctac cgttctagga ccatagccag acgctaaggc tgagcaggtt gtagttatgt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acctattaat ttcccctcgt caaaaataag gttatcaagc gagaaatcac catgagtgac 1680

1621 tggataatta aaggggagca gtttttattc caatagttcg ctctttagtg gtactcactg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gactgaatcc ggtgagaatg gcaaaagcta atgcatagct cctgaaaatc tcgataactc 1740

1681 ctgacttagg ccactcttac cgttttcgat tacgtatcga ggacttttag agctattgag 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg 1800

1741 ttttttatgc gggccatcac tagaataaag taataccact ttcaaccttg gagaatgcac 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac 1860

1801 ggctagttgc agagtaaaag cggttttcaa ccgggtcccg aagggccata gttgtccctg 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cgaagacgaa 1920

1861 tggtcctaaa taaataagac gcttcactag aaggcagtgt ccataaataa gcttctgctt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 1980

1921 tcccggagca ctatgcggat aaaaatatcc aattacagta ctattattac caaagaatct 1980

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

1981 cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc gctgggcctg 2040

1981 gcagtccacc gtgaaaagcc cctttacacg cgcgggcgca aggacgaccg cgacccggac 2040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg ccttgatgat 2100

2041 aaagaccgcg acctgaaggg cgacaaggca gtcgtcgaaa agcgggtgcc ggaactacta 2100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca gaacgggctt 2160

2101 gcgccgccgg aaccggacgt atagggctaa gttgccgggg tcccgcaggt cttgcccgaa 2160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caggcgctcc cgaaggtctc gggccgtctc ttgggcttga tcggccttct tgcgcatctc 2220

2161 gtccgcgagg gcttccagag cccggcagag aacccgaact agccggaaga acgcgtagag 2220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acgcgctcct gcggcggcct gtagggcagg ctcatacccc tgccgaaccg cttttgtcag 2280

2221 tgcgcgagga cgccgccgga catcccgtcc gagtatgggg acggcttggc gaaaacagtc 2280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccggtcggcc acggcttccg gcgtctcaac gcgctttgag attcccagct tttcggccaa 2340

2281 ggccagccgg tgccgaaggc cgcagagttg cgcgaaactc taagggtcga aaagccggtt 2340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tccctgcggt gcataggcgc gtggctcgac cgcttgcggg ctgatggtga cgtggcccac 2400

2341 agggacgcca cgtatccgcg caccgagctg gcgaacgccc gactaccact gcaccgggtg 2400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tggtggccgc tccagggcct cgtagaacgc ctgaatgcgc gtgtgacgtg ccttgctgcc 2460

2401 accaccggcg aggtcccgga gcatcttgcg gacttacgcg cacactgcac ggaacgacgg 2460

.......... .......... ORF 2 .......... .......... ..........

.......... ......... SfiI(2496),NotI(2497),EagI(2497) .........

2461 ctcgatgccc cgttgcagcc ctagatcggc cacagcggcc gcaaacgtgg tctggtcgcg 2520

2461 gagctacggg gcaacgtcgg gatctagccg gtgtcgccgg cgtttgcacc agaccagcgc 2520

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtcatctgc gctttgttgc cgatgaactc cttggccgac agcctgccgt cctgcgtcag 2580

2521 ccagtagacg cgaaacaacg gctacttgag gaaccggctg tcggacggca ggacgcagtc 2580

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cggcaccacg aacgcggtca tgtgcgggct ggtttcgtca cggtggatgc tggccgtcac 2640

2581 gccgtggtgc ttgcgccagt acacgcccga ccaaagcagt gccacctacg accggcagtg 2640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gatgcgatcc gccccgtact tgtccgccag ccacttgtgc gccttctcga agaacgccgc 2700

2641 ctacgctagg cggggcatga acaggcggtc ggtgaacacg cggaagagct tcttgcggcg 2700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ctgctgttct tggctggccg acttccacca ttccgggctg gccgtcatga cgtactcgac 2760

2701 gacgacaaga accgaccggc tgaaggtggt aaggcccgac cggcagtact gcatgagctg 2760

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgccaacaca gcgtccttgc gccgcttctc tggcagcaac tcgcgcagtc ggcccatcgc 2820

2761 gcggttgtgt cgcaggaacg cggcgaagag accgtcgttg agcgcgtcag ccgggtagcg 2820

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcatcggtg ctgctggccg cccagtgctc gttctctggc gtcctgctgg cgtcagcgtt 2880

2821 aagtagccac gacgaccggc gggtcacgag caagagaccg caggacgacc gcagtcgcaa 2880

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gggcgtctcg cgctcgcggt aggcgtgctt gagactggcc gccacgttgc ccattttcgc 2940

2881 cccgcagagc gcgagcgcca tccgcacgaa ctctgaccgg cggtgcaacg ggtaaaagcg 2940

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cagcttcttg catcgcatga tcgcgtatgc cgccatgcct gcccctccct tttggtgtcc 3000

2941 gtcgaagaac gtagcgtact agcgcatacg gcggtacgga cggggaggga aaaccacagg 3000

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aaccggctcg acgggggcag cgcaaggcgg tgcctccggc gggccactca atgcttgagt 3060

3001 ttggccgagc tgcccccgtc gcgttccgcc acggaggccg cccggtgagt tacgaactca 3060

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atactcacta gactttgctt cgcaaagtcg tgaccgccta cggcggctgc ggcgccctac 3120

3061 tatgagtgat ctgaaacgaa gcgtttcagc actggcggat gccgccgacg ccgcgggatg 3120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc tcccttgcca gcccgtggat 3180

3121 cccgaacgag aggcccgaag cgggacgcgc cagcgacgcg agggaacggt cgggcaccta 3180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atgtggacga tggccgcgag cggccaccgg ctggctcgct tcgctcggcc cgtggacaac 3240

3181 tacacctgct accggcgctc gccggtggcc gaccgagcga agcgagccgg gcacctgttg 3240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cctgctggac aagctgatgg acaggctgcg cctgcccacg agcttgacca cagggattgc 3300

3241 ggacgacctg ttcgactacc tgtccgacgc ggacgggtgc tcgaactggt gtccctaacg 3300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ccaccggcta cccagccttc gaccacatac ccaccggctc caactgcgcg gcctgcggcc 3360

3301 ggtggccgat gggtcggaag ctggtgtatg ggtggccgag gttgacgcgc cggacgccgg 3360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttgccccatc aattttttta attttctctg gggaaaagcc tccggcctgc ggcctgcgcg 3420

3361 aacggggtag ttaaaaaaat taaaagagac cccttttcgg aggccggacg ccggacgcgc 3420

ORF 2 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca gcgaccgcgc 3480

3421 gaagcgaacg gccaacctgt ggttcacctt ccgcccagtt ccgagcgcgt cgctggcgcg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agcggcttgg ccttgacgcg cctggaacga cccaagccta tgcgagtggg ggcagtcgaa 3540

3481 tcgccgaacc ggaactgcgc ggaccttgct gggttcggat acgctcaccc ccgtcagctt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg gttccaaggg 3600

3541 ccgcttcggg cgggcggacg gggggctcgg agtgccgccg ctcacgcccc caaggttccc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc cccggagggg 3660

3601 ccgtcgcggt ggaacccgtt ccggcttccg gcgcgtcagc tagttgttcg gggcctcccc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc aggggtgccc 3720

3661 ggtgaaaaac ggcctccccc tcggcgcggc ttccgcaccc ccttggggcg tccccacggg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa aaatcaacaa 3780

3721 aagaaacccg tggtttcttg atctatatcc cgctttacgc tttctgaatt tttagttgtt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag ctcattgcgt 3840

3781 gaattttttc cccccatgcg ttgtcgagta acgccgtggg gggcgttatc gagtaacgca 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt gttgtcgcgc 3900

3841 tccaatttct tttagacatt aactgacggt gaaaatgcgt tgcgtattaa caacagcgcg 3900

.......... .......... .......... .......... ORF 1 ..........

......... PvuII(3917) ......... .......... .......... ..........

3901 tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc ctaccgcatg 3960

3901 acggcttttc aacgtcgact aacgcgtacc acggcgttgg cacgccgtgg gatggcgtac 3960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gagataagca tggccacgca gtccagagaa atcggcattc aagccaagaa caagcccggt 4020

3961 ctctattcgt accggtgcgt caggtctctt tagccgtaag ttcggttctt gttcgggcca 4020

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cactgggtgc aaacggaacg caaagcgcat gaggcgtggg ccgggcttat tgcgaggaaa 4080

4021 gtgacccacg tttgccttgc gtttcgcgta ctccgcaccc ggcccgaata acgctccttt 4080

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cccacggcgg caatgctgct gcatcacctc gtggcgcaga tgggccacca gaacgccgtg 4140

4081 gggtgccgcc gttacgacga cgtagtggag caccgcgtct acccggtggt cttgcggcac 4140

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtggtcagcc agaagacact ttccaagctc atcggacgtt ctttgcggac ggtccaatac 4200

4141 caccagtcgg tcttctgtga aaggttcgag tagcctgcaa gaaacgcctg ccaggttatg 4200

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcagtcaagg acttggtggc cgagcgctgg atctccgtcg tgaagctcaa cggccccggc 4260

4201 cgtcagttcc tgaaccaccg gctcgcgacc tagaggcagc acttcgagtt gccggggccg 4260

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg gccagccccg cgaccagttg 4320

4261 tggcacagcc ggatgcacca gttactggcg caccgcaccc cggtcggggc gctggtcaac 4320

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... BclI(4354) .......... ..........

4321 cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg acgaccagga cgaatcgctg 4380

4321 gcggacagcc acaagtcacg gcggcaccac caactagtgc tgctggtcct gcttagcgac 4380

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ttggggcatg gcgacctgcg ccgcatcccg accctgtatc cgggcgagca gcaactaccg 4440

4381 aaccccgtac cgctggacgc ggcgtagggc tgggacatag gcccgctcgt cgttgatggc 4440

ORF 1 .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 accggccccg gcgaggagcc gcccagccag cccggcattc cgggcatgga accagacctg 4500

4441 tggccggggc cgctcctcgg cgggtcggtc gggccgtaag gcccgtacct tggtctggac 4500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct gccgatgccc 4560

4501 ggtcggaact ggctttgcct ccttaccctt gccgcgcccg tcgtcgcgga cggctacggg 4560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gatgagccgt gttttctgga cgatggcgag ccgttggagc cgccgacacg ggtcacgctg 4620

4561 ctactcggca caaaagacct gctaccgctc ggcaacctcg gcggctgtgc ccagtgcgac 4620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccgcgccggt agcacttggg ttgcgcagca acccgtaagt gcgctgttcc agactatcgg 4680

4621 ggcgcggcca tcgtgaaccc aacgcgtcgt tgggcattca cgcgacaagg tctgatagcc 4680

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 4740

4681 gacatcggcg gagcggcggg atatggaaca gacggagggg cgcaacgcag cgccacgtac 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccgtt 4800

4741 ctcggcccgg tggagctgga cttaccttcg gccgccgtgg agcgattgcc taagtggcaa 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg 4860

4801 aaatagtccg agaccctccg tcttatttac tagtatagca gttaataatg gaggtgcccc 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta 4920

4861 tctcggactc gtttgaccgg agtccgtaaa ctcttcgtgt gccagtgtga cgaaggccat 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa 4980

4921 cagttatttg gccatttggt cgttatctgt attcgccgat aaattgctgg gacgggactt 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta 5040

4981 ggctgctggc ccagcttaaa cgaaagctta aagacggtaa gtaggcgaat aatagtgaat 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttcaggcgta gcaccaggcg tttaagtcga ccaataactc tacggggtct gacgctcagt 5100

5041 aagtccgcat cgtggtccgc aaattcagct ggttattgag atgccccaga ctgcgagtca 5100

.......... .......... .......... .......... .......... ..........

.......... ClaI(5111) ScaI(5125) .......... .......... ..........

5101 ggaacgaaat cgattcatcg cagtactgtt gtattcatta agcatctgcc gacatggaag 5160

5101 ccttgcttta gctaagtagc gtcatgacaa cataagtaat tcgtagacgg ctgtaccttc 5160

.......... .......... .......... CAT .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 5220

5161 ggtagtgttt gccgtactac ttggacttag cggtcgccgt agtcgtggaa cagcggaacg 5220

.......... .......... CAT .......... .......... ..........

.......... NcoI(5235) .......... .......... .......... ..........

5221 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 5280

5221 catattataa acgggtacca cttttgcccc cgcttcttca acaggtataa ccggtgcaaa 5280

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 5340

5281 tttagttttg accactttga gtgggtccct aaccgactct gctttttgta taagagttat 5340

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 5400

5341 ttgggaaatc cctttatccg gtccaaaagt ggcattgtgc ggtgtagaac gcttatatac 5400

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 5460

5401 acatctttga cggcctttag cagcaccata agtgaggtct cgctactttt gcaaagtcaa 5460

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 5520

5461 acgagtacct tttgccacat tgttcccact tgtgataggg tatagtggtc gagtggcaga 5520

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ttcattgcca tacgtaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 5580

5521 aagtaacggt atgcattaag gcctactcgt aagtagtccg cccgttctta cacttatttc 5580

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 5640

5581 cggcctattt tgaacacgaa taaaaagaaa tgccagaaat ttttccggca ttataggtcg 5640

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 5700

5641 acttgccaga ccaatatcca tgtaactcgt tgactgactt tacggagttt tacaagaaat 5700

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 5760

5701 gctacggtaa ccctatatag ttgccaccat ataggtcact aaaaaaagag gtaaaatcga 5760

.......... .......... CAT .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tccttagctc ctgaaaatct cgacaactca aaaaatacgc ccggtagtga tcttatttca 5820

5761 aggaatcgag gacttttaga gctgttgagt tttttatgcg ggccatcact agaataaagt 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 5880

5821 aataccactt tcaaccttgg agaatgcacg gctagttgca gagtaaaagc ggttttcaac 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 5940

5881 cgggtcccga agggccatag ttgtccctgt ggtcctaaat aaataagacg cttcactaga 5940

.......... .......... .......... .......... .......... ..........

.......... AscI(5956) .......... ClaI(5979) .......... ..........

5941 tccgtcacag gtaggcgcgc cgaagttcct atacttatcg atctgcactt gaacgtgtgg 6000

5941 aggcagtgtc catccgcgcg gcttcaagga tatgaatagc tagacgtgaa cttgcacacc 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 cctaagcgat .......... .......... .......... .......... .......... 6010

6001 ggattcgcta .......... .......... .......... .......... .......... 6010

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-6010 Cross-reference: DOI; 10.1128/JB.00481-08. Cross-reference: PUBMED; 18776009. Reference author: Law R.J., Hamlin J.N., Sivro A., McCorrister S.J., Cardama G.A., Reference author: Cardona S.T.; Reference title: "A functional phenylacetic acid catabolic pathway is required for full Reference title: pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans Reference title: host model"; Reference location: J. Bacteriol. 190(21):7209-7218(2008).
Reference number: [2] Reference positions: 1-6010 Reference author: Law R.J., Hamlin J.N.R., Sivro A., McCorrister S.J., Cardama G., Reference author: Cardona S.T.; Reference title: ; Reference location: Submitted (02-APR-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Microbiology, University of Manitoba, 45 Chancellors Circle, 418 Buller Reference location: Bldg, Winnipeg, Manitoba R3T 2N2, Canada