Sequence of pAQJ4-MCS(C)

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Sequence of pAQJ4-MCS(C)

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Sequence of pAQJ4-MCS(C): 5878 BP; 1398 A; 1492 C; 1485 G; 1503 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

1 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 60

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

61 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

121 aaccgcccac agccccgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240

181 tggtatacgc cacactttat ggcgtgtcta cgcattcctc ttttatggcg tagtccgcgg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300

241 taagcggtaa gtccgacgcg ttgacaaccc ttcccgctag ccacgcccgg agaagcgata 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360

301 atgcggtcga ccgctttccc cctacacgac gttccgctaa ttcaacccat tgcggtccca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... EcoRI(397) ClaI(404) ..........

361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt catcgatggt acccgggatc 420

361 aaagggtcag tgctgcaaca ttttgctgcc ggtcacttaa gtagctacca tgggccctag 420

. M13_pUC_fwd_primer M13_forward20_primer . .......... ..........

PstI(427),XbaI(429) SacI(444) .......... .......... ..........

421 cctgcagtct agaaattcga gctcatgccc ccggtagtgc cctcaccctc agtgaggctt 480

421 ggacgtcaga tctttaagct cgagtacggg ggccatcacg ggagtgggag tcactccgaa 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atgatctggc acagtctagg ggctaccaga agagcaaagg cgctttccgc aagggcataa 540

481 tactagaccg tgtcagatcc ccgatggtct tctcgtttcc gcgaaaggcg ttcccgtatt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aacgtcccga taccgcagag caaatcttga gattgtttgg gattgccaag ggagagctgc 600

541 ttgcagggct atggcgtctc gtttagaact ctaacaaacc ctaacggttc cctctcgacg 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acaacgagta tttcgatcac gggtctaagc ctcctgaata aatctattta tacaggggtt 660

601 tgttgctcat aaagctagtg cccagattcg gaggacttat ttagataaat atgtccccaa 660

.......... .......... .......... .......... orf943 ..........

.......... .......... .......... .......... .......... ..........

661 ggacacggcc cctaattttg cttggtcacg ctgtaaccaa tgagcaaaga ccttttcgcg 720

661 cctgtgccgg ggattaaaac gaaccagtgc gacattggtt actcgtttct ggaaaagcgc 720

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctgatcgtta ggggcgatcg cctcatagac ccgctgacgg ttatcccgcg atccaaagcg 780

721 gactagcaat ccccgctagc ggagtatctg ggcgactgcc aatagggcgc taggtttcgc 780

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccccttgtat tccaatttcc ggtcaagctt gcctaggagc gcctgagcaa tttcgagcgg 840

781 ggggaacata aggttaaagg ccagttcgaa cggatcctcg cggactcgtt aaagctcgcc 840

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gctcattttg tcggtgatcg ttctgccaag aatccacttg atcggcttgg cataccgcaa 900

841 cgagtaaaac agccactagc aagacggttc ttaggtgaac tagccgaacc gtatggcgtt 900

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 acaattttcc ttgaaccctt caaggctcgc cccggtgaat gtcacccctg gttttaagaa 960

901 tgttaaaagg aacttgggaa gttccgagcg gggccactta cagtggggac caaaattctt 960

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ctgatggatg ttgagtagct ctaacaggtg aatctttggt gagagcgatc gcctgttgat 1020

961 gactacctac aactcatcga gattgtccac ttagaaacca ctctcgctag cggacaacta 1020

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 atccggctta aatacaaagc cctcatgttg gagctggtca tatttcgccc ggtcgcgggc 1080

1021 taggccgaat ttatgtttcg ggagtacaac ctcgaccagt ataaagcggg ccagcgcccg 1080

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agagcagaat gctttcccaa cggttaagta gtattcgagc tggagctgag agtaccaccc 1140

1081 tctcgtctta cgaaagggtt gccaattcat cataagctcg acctcgactc tcatggtggg 1140

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... HpaI(1178) .......... ..........

1141 atcatcatct ttctcgacaa gctcaggggt cacagttaac ccataggagc gagaaagttt 1200

1141 tagtagtaga aagagctgtt cgagtcccca gtgtcaattg ggtatcctcg ctctttcaaa 1200

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cccgtggcgt tcttggtgcc gttccgtctc agatttcgcc cgctgacgct tcagtttttc 1260

1201 gggcaccgca agaaccacgg caaggcagag tctaaagcgg gcgactgcga agtcaaaaag 1260

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gtactggcga tcgtcaggat tttctacttt agaaatcgca accctttcct gtagataatt 1320

1261 catgaccgct agcagtccta aaagatgaaa tctttagcgt tgggaaagga catctattaa 1320

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atggtctttc accgctttta attcgtctcg ataatcctta gccaatgctg gatcgatttc 1380

1321 taccagaaag tggcgaaaat taagcagagc tattaggaat cggttacgac ctagctaaag 1380

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gctcaaatct ttctgtacat agccctcaga aagcagctta tttaaaatcg cttcccggta 1440

1381 cgagtttaga aagacatgta tcgggagtct ttcgtcgaat aaattttagc gaagggccat 1440

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 actgccaaag ccacggttag caagcgccgc atagcgacaa taggcccaaa ggaatggttt 1500

1441 tgacggtttc ggtgccaatc gttcgcggcg tatcgctgtt atccgggttt ccttaccaaa 1500

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 taaagaatct tctaacccac tgcattcagc ggcgtgctca ccgattaatt tgtgggtgag 1560

1501 atttcttaga agattgggtg acgtaagtcg ccgcacgagt ggctaattaa acacccactc 1560

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctctgcatag cggtgctggt cacgggcgat cgccttagcg gtgtagcccc cattggcgat 1620

1561 gagacgtatc gccacgacca gtgcccgcta gcggaatcgc cacatcgggg gtaaccgcta 1620

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccggttcgag tgtggggaaa atttcggtat ccaaacatgg cgagggacgt tatcccgtaa 1680

1621 ggccaagctc acaccccttt taaagccata ggtttgtacc gctccctgca atagggcatt 1680

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccgctctagc ccttggcaga aaccgttaac ggtctgaacc cctgaaccca tgccccaaac 1740

1681 ggcgagatcg ggaaccgtct ttggcaattg ccagacttgg ggacttgggt acggggtttg 1740

.......... .......... orf943 .......... .......... ..........

.......... .... EcoRV(1762),ClaI(1764) .... .......... ..........

1741 agaatcaaaa tgatccttga tatcgatact caccccggtc tcgacagcgg gcgagcagag 1800

1741 tcttagtttt actaggaact atagctatga gtggggccag agctgtcgcc cgctcgtctc 1800

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cacaatatcg tacaggggca aaattgcatt cagcgagtca atacaaccat aggcagggtg 1860

1801 gtgttatagc atgtccccgt tttaacgtaa gtcgctcagt tatgttggta tccgtcccac 1860

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccccggttca gcgaccgatt cagcatcgat tctcaagatt ctcttatcgg gatatttctc 1920

1861 ggggccaagt cgctggctaa gtcgtagcta agagttctaa gagaatagcc ctataaagag 1920

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cctaaagagt gattctaagt tctgcgtgct gtaggtcgat gcggtctttt gggcagcggt 1980

1921 ggatttctca ctaagattca agacgcacga catccagcta cgccagaaaa cccgtcgcca 1980

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aaagacaagt gttttctcac cgttctcaat cgcttgtctg agattggtta acaggtctct 2040

1981 tttctgttca caaaagagtg gcaagagtta gcgaacagac tctaaccaat tgtccagaga 2040

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agggtcattc ccttcataga aaaacaggtc tctttgttgc tctaaacagg gcttataggt 2100

2041 tcccagtaag ggaagtatct ttttgtccag agaaacaacg agatttgtcc cgaatatcca 2100

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 attcagaatg gtgaatggtt tgaactcaca accgtcaaga atgtacttga ttagctcata 2160

2101 taagtcttac cacttaccaa acttgagtgt tggcagttct tacatgaact aatcgagtat 2160

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... ......... Bsu36I(2211)

2161 ggagatggga gataaatcag catcagagca gtagagtttt ccgcctgtcc ttagggcata 2220

2161 cctctaccct ctatttagtc gtagtctcgt catctcaaaa ggcggacagg aatcccgtat 2220

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aaggattagg ttgtagaacg tttcaaggat tttcgcccgg tcttgggtac aggtcgagct 2280

2221 ttcctaatcc aacatcttgc aaagttccta aaagcgggcc agaacccatg tccagctcga 2280

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acccaaagcg tgccctaaaa cttgctcgat ttcgtctagg acaatgtgag cgccgtgcca 2340

2281 tgggtttcgc acgggatttt gaacgagcta aagcagatcc tgttacactc gcggcacggt 2340

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttccatgaaa ttaaaatgag cttggctatc gggatgtagg ctatcgatac acatcgccat 2400

2341 aaggtacttt aattttactc gaaccgatag ccctacatcc gatagctatg tgtagcggta 2400

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... SacI(2436) .......... ..........

2401 ccctagagcg cccccggtcg ggctactgtc gagctcgcta atgtgatcga tatcgagacg 2460

2401 gggatctcgc gggggccagc ccgatgacag ctcgagcgat tacactagct atagctctgc 2460

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcgggcgagc tccttggcta attggatgcg gtgagtcaaa accagtacgc gagcgcaatc 2520

2461 cgcccgctcg aggaaccgat taacctacgc cactcagttt tggtcatgcg ctcgcgttag 2520

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atcttgggcg agcttaacga ttttccctaa ccattctgtt ttcccggtgc ccttggcaga 2580

2521 tagaacccgc tcgaattgct aaaagggatt ggtaagacaa aagggccacg ggaaccgtct 2580

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tttaatgaaa ataatctgag catccttggg gatagcacgc ggatcgatgt cgagatagcg 2640

2581 aaattacttt tattagactc gtaggaaccc ctatcgtgcg cctagctaca gctctatcgc 2640

.......... .......... orf943 .......... .......... ..........

.......... .......... ......... EcoRV(2674) ......... ..........

2641 ctgatcgacc gttagatcga cttgcgtggt gatatcggtg agattatcca tctcccagac 2700

2641 gactagctgg caatctagct gaacgcacca ctatagccac tctaataggt agagggtctg 2700

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgctaagggc ttgcggtgct tgtagcggtt atggagtgct ttcgctccgt gctcgacgat 2760

2701 acgattcccg aacgccacga acatcgccaa tacctcacga aagcgaggca cgagctgcta 2760

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cagatcatca acacctttcg cgtcctggtg ccaggtgatc accgatactt tcgccccggc 2820

2761 gtctagtagt tgtggaaagc gcaggaccac ggtccactag tggctatgaa agcggggccg 2820

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cttctctagg agtgccccgg tagtttggat cgccccgttc actgccttga tcgtcttggg 2880

2821 gaagagatcc tcacggggcc atcaaaccta gcggggcaag tgacggaact agcagaaccc 2880

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tttcttgtct tggtcaaaac agaaggcgat cgcccggtta tttttcgcca acaggtcaag 2940

2881 aaagaacaga accagttttg tcttccgcta gcgggccaat aaaaagcggt tgtccagttc 2940

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctctggaatg aggtgccgta gctgtcgcat tggctcgcca tggtcatttt ttggcgttct 3000

2941 gagaccttac tccacggcat cgacagcgta accgagcggt accagtaaaa aaccgcaaga 3000

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(3059)

3001 gtagccgttg taaatccccg gtagaccgat ggcgcaataa ccagcggtta ggagggcccc 3060

3001 catcggcaac atttaggggc catctggcta ccgcgttatt ggtcgccaat cctcccgggg 3060

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgccttcttc gcgccctcag tgatgatgat cggtagctca ggatgggcta gtacccattc 3120

3061 acggaagaag cgcgggagtc actactacta gccatcgagt cctacccgat catgggtaag 3120

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccagaagcct tgatcggttt ctggtagctc gactttgtgg cgcttggcaa tcttgcgcca 3180

3121 ggtcttcgga actagccaaa gaccatcgag ctgaaacacc gcgaaccgtt agaacgcggt 3180

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggtgccccgg tctaccttga gagtgaaaat ttcggtggct acccgtggcg ggtgctcata 3240

3181 ccacggggcc agatggaact ctcactttta aagccaccga tgggcaccgc ccacgagtat 3240

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cttgatcggt ttcttatctt cgcgatcttt acgggggcga tcgcctttga agcaacccca 3300

3241 gaactagcca aagaatagaa gcgctagaaa tgcccccgct agcggaaact tcgttggggt 3300

.......... .......... orf943 .......... .......... ..........

.......... .......... ClaI(3327) .......... .......... ..........

3301 aagagaatct ttcccggtct taatatcgat gcctgagcac caccaaccgc catgctcgac 3360

3301 ttctcttaga aagggccaga attatagcta cggactcgtg gtggttggcg gtacgagctg 3360

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaaggcatat cgcttaagcc aagtatcccg aagcctgcca tcattgcggc gtaatgctgg 3420

3361 tttccgtata gcgaattcgg ttcatagggc ttcggacggt agtaacgccg cattacgacc 3420

.......... .......... orf943 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctcatcactg tagagcaggt attcataggg cgtcatccct gacaacgatc taacatttaa 3480

3421 gagtagtgac atctcgtcca taagtatccc gcagtaggga ctgttgctag attgtaaatt 3480

.......... orf943 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccgggcaatc tcaggggcga ccccggaacc ctctacccat tccctatagt gagattctgc 3540

3481 ggcccgttag agtccccgct ggggccttgg gagatgggta agggatatca ctctaagacg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gataaaatga gaacggttct cactgacgct actcgctaaa tctgccccgg tattactgct 3600

3541 ctattttact cttgccaaga gtgactgcga tgagcgattt agacggggcc ataatgacga 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agatgtaaaa ttatctgata cgatcacagt gagttaagga gcttggcgta atcatggtca 3660

3601 tctacatttt aatagactat gctagtgtca ctcaattcct cgaaccgcat tagtaccagt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 3720

3661 atcgacaaag gacacacttt aacaataggc gagtgttaag gtgtgttgta tgctcggcct 3720

M13_reverse_primer M13_pUC_rev_primer .... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

3721 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 3780

3721 tcgtatttca catttcggac cccacggatt actcactcga ttgagtgtaa ttaacgcaac 3780

lac_promoter ........ .......... .......... .......... ..........

.......... .......... .......... ......... PvuII(3823) .........

3781 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 3840

3781 gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg tcgacgtaat tacttagccg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 3900

3841 gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa ggcgaaggag cgagtgactg 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3960

3901 agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4020

3961 gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4080

4021 ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 4140

4081 ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 4200

4141 tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 4260

4201 gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag agtatcgagt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4320

4261 gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4380

4321 ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4440

4381 cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 4500

4441 atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttcc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4560

4501 tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4620

4561 agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4680

4621 taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4740

4681 cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag tttttcctag 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4800

4741 aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc atatatactc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4860

4801 atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag tcgctagaca 4860

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4861 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4920

4861 gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg ctatgccctc 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4980

4921 ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag tggccgaggt 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 5040

4981 ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc aggacgttga 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 5100

5041 aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc atcaagcggt 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 5160

5101 caattatcaa acgcgttgca acaacggtaa cgatgtccgt agcaccacag tgcgagcagc 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 5220

5161 aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg tactaggggg 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 5280

5221 tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc ttcattcaac 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 5340

5281 cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg acagtacggt 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... ScaI(5372) .......... ..........

5341 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 5400

5341 aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac tcttatcaca 5400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 5460

5401 tacgccgctg gctcaacgag aacgggccgc agttatgccc tattatggcg cggtgtatcg 5460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 5520

5461 tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga gagttcctag 5520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 5580

5521 aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac tagaagtcgt 5580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 5640

5581 agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt acggcgtttt 5640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 5700

5641 ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa agttataata 5700

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 5760

5701 acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt 5760

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

5761 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 5820

5761 ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact gcagattctt 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc.. 5878

5821 tggtaataat agtactgtaa ttggatattt ttatccgcat agtgctccgg gaaagcag.. 5878

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-5878 Reference author: Miyasaka H., Tanaka S., Onizuka T., Akiyama H.; Reference title: ; Reference location: Submitted (04-FEB-2009) to the EMBL/GenBank/DDBJ databases. Reference location: Contact:Takuo Onizuka Toray Research Center, Inc., Biological Science Reference location: Laboratories; 1111 Tebiro, Kamakura, Kanagawa 248-0036, Japan
Reference number: [2] Reference author: Miyasaka H., Tanaka S., Onizuka T., Akiyama H.; Reference title: "Cyanobacterial shuttle vector pAQJ4-MCS(C)"; Reference location: Unpublished.
Reference number: [3] Cross-reference: PUBMED; 15564522. Reference author: Onizuka T., Endo S., Akiyama H., Kanai S., Hirano M., Yokota A., Tanaka S., Reference author: Miyasaka H.; Reference title: "The rbcX gene product promotes the production and assembly of Reference title: ribulose-1,5-bisphosphate carboxylase/oxygenase of Synechococcus sp. Reference title: PCC7002 in Escherichia coli"; Reference location: Plant Cell Physiol. 45(10):1390-1395(2004).