~,ID AB480231; SV 1; circular; other DNA; STD; SYN; 5878 BP. AC AB480231; DT 10-FEB-2009 (Rel. 99, Created) DT 10-FEB-2009 (Rel. 99, Last updated, Version 1) DE Cyanobacterial shuttle vector pAQJ4-MCS DNA, complete sequence. KW . OS Cyanobacterial shuttle vector pAQJ4-MCS OC other sequences; artificial sequences; vectors. RN [1] RP 1-5878 RA Miyasaka H., Tanaka S., Onizuka T., Akiyama H.; RT ; RL Submitted (04-FEB-2009) to the EMBL/GenBank/DDBJ databases. RL Contact:Takuo Onizuka Toray Research Center, Inc., Biological Science RL Laboratories; 1111 Tebiro, Kamakura, Kanagawa 248-0036, Japan RN [2] RA Miyasaka H., Tanaka S., Onizuka T., Akiyama H.; RT "Cyanobacterial shuttle vector pAQJ4-MCS"; RL Unpublished. RN [3] RX PUBMED; 15564522. RA Onizuka T., Endo S., Akiyama H., Kanai S., Hirano M., Yokota A., Tanaka S., RA Miyasaka H.; RT "The rbcX gene product promotes the production and assembly of RT ribulose-1,5-bisphosphate carboxylase/oxygenase of Synechococcus sp. RT PCC7002 in Escherichia coli"; RL Plant Cell Physiol. 45(10):1390-1395(2004). FH Key Location/Qualifiers FT source 1..5878 FT /organism="Cyanobacterial shuttle vector pAQJ4-MCS" FT /mol_type="other DNA" FT /note="derived from Cloning vector pUC19 and Synechococcus FT sp. PCC 7002 plasmid pAQ1 (D13972)" FT /db_xref="taxon:595658" FT misc_feature 401..406 FT /note="XbaI cloning site" FT misc_feature 407..412 FT /note="PstI cloning site" FT misc_feature 413..418 FT /note="BamHI cloning site" FT misc_feature 417..422 FT /note="SmaI cloning site" FT misc_feature 421..426 FT /note="KpnI cloning site" FT misc_feature 427..432 FT /note="ClaI cloning site" FT misc_feature 433..438 FT /note="EciRI cloning site" FT CDS complement(625..3456) FT /codon_start=1 FT /transl_table=11 FT /gene="orf943" FT /product="ORF943" FT /function="essential for the replication of the plasmid in FT cyanobacteria" FT /protein_id="BAH22361.1" FT /translation="MTPYEYLLYSDEPALRRNDGRLRDTWLKRYAFVEHGGWWCSGIDI FT KTGKDSLWGCFKGDRPRKDREDKKPIKYEHPPRVATEIFTLKVDRGTWRKIAKRHKVEL FT PETDQGFWEWVLAHPELPIIITEGAKKAGALLTAGYCAIGLPGIYNGYRTPKNDHGEPM FT RQLRHLIPELDLLAKNNRAIAFCFDQDKKPKTIKAVNGAIQTTGALLEKAGAKVSVITW FT HQDAKGVDDLIVEHGAKALHNRYKHRKPLAVWEMDNLTDITTQVDLTVDQRYLDIDPRA FT IPKDAQIIFIKSAKGTGKTEWLGKIVKLAQDDCARVLVLTHRIQLAKELARRLDIDHIS FT ELDSSPTGGALGMAMCIDSLHPDSQAHFNFMEWHGAHIVLDEIEQVLGHALGSSTCTQD FT RAKILETFYNLILYALRTGGKLYCSDADLSPISYELIKYILDGCEFKPFTILNTYKPCL FT EQQRDLFFYEGNDPRDLLTNLRQAIENGEKTLVFTAAQKTASTYSTQNLESLFREKYPD FT KRILRIDAESVAEPGHPAYGCIDSLNAILPLYDIVLCSPAVETGVSIDIKDHFDSVWGM FT GSGVQTVNGFCQGLERLRDNVPRHVWIPKFSPHSNRIANGGYTAKAIARDQHRYAELTH FT KLIGEHAAECSGLEDSLKPFLWAYCRYAALANRGFGSYREAILNKLLSEGYVQKDLSEI FT DPALAKDYRDELKAVKDHNYLQERVAISKVENPDDRQYEKLKRQRAKSETERHQERHGK FT LSRSYGLTVTPELVEKDDDGWYSQLQLEYYLTVGKAFCSARDRAKYDQLQHEGFVFKPD FT INRRSLSPKIHLLELLNIHQFLKPGVTFTGASLEGFKENCLRYAKPIKWILGRTITDKM FT SPLEIAQALLGKLDRKLEYKGRFGSRDNRQRVYEAIAPNDQREKVFAHWLQRDQAKLGA FT VSNPCINRFIQEA" SQ Sequence 5878 BP; 1398 A; 1491 C; 1486 G; 1503 T; 0 other; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt tctagactgc agggatcccg 420 ggtaccatcg atgaattcga gctcatgccc ccggtagtgc cctcaccctc agtgaggctt 480 atgatctggc acagtctagg ggctaccaga agagcaaagg cgctttccgc aagggcataa 540 aacgtcccga taccgcagag caaatcttga gattgtttgg gattgccaag ggagagctgc 600 acaacgagta tttcgatcac gggtctaagc ctcctgaata aatctattta tacaggggtt 660 ggacacggcc cctaattttg cttggtcacg ctgtaaccaa tgagcaaaga ccttttcgcg 720 ctgatcgtta ggggcgatcg cctcatagac ccgctgacgg ttatcccgcg atccaaagcg 780 ccccttgtat tccaatttcc ggtcaagctt gcctaggagc gcctgagcaa tttcgagcgg 840 gctcattttg tcggtgatcg ttctgccaag aatccacttg atcggcttgg cataccgcaa 900 acaattttcc ttgaaccctt caaggctcgc cccggtgaat gtcacccctg gttttaagaa 960 ctgatggatg ttgagtagct ctaacaggtg aatctttggt gagagcgatc gcctgttgat 1020 atccggctta aatacaaagc cctcatgttg gagctggtca tatttcgccc ggtcgcgggc 1080 agagcagaat gctttcccaa cggttaagta gtattcgagc tggagctgag agtaccaccc 1140 atcatcatct ttctcgacaa gctcaggggt cacagttaac ccataggagc gagaaagttt 1200 cccgtggcgt tcttggtgcc gttccgtctc agatttcgcc cgctgacgct tcagtttttc 1260 gtactggcga tcgtcaggat tttctacttt agaaatcgca accctttcct gtagataatt 1320 atggtctttc accgctttta attcgtctcg ataatcctta gccaatgctg gatcgatttc 1380 gctcaaatct ttctgtacat agccctcaga aagcagctta tttaaaatcg cttcccggta 1440 actgccaaag ccacggttag caagcgccgc atagcgacaa taggcccaaa ggaatggttt 1500 taaagaatct tctaacccac tgcattcagc ggcgtgctca ccgattaatt tgtgggtgag 1560 ctctgcatag cggtgctggt cacgggcgat cgccttagcg gtgtagcccc cattggcgat 1620 ccggttcgag tgtggggaaa atttcggtat ccaaacatgg cgagggacgt tatcccgtaa 1680 ccgctctagc ccttggcaga aaccgttaac ggtctgaacc cctgaaccca tgccccaaac 1740 agaatcaaaa tgatccttga tatcgatact caccccggtc tcgacagcgg gcgagcagag 1800 cacaatatcg tacaggggca aaattgcatt cagcgagtca atacaaccat aggcagggtg 1860 ccccggttca gcgaccgatt cagcatcgat tctcaagatt ctcttatcgg gatatttctc 1920 cctaaagagt gattctaagt tctgcgtgct gtaggtcgat gcggtctttt gggcagcggt 1980 aaagacaagt gttttctcac cgttctcaat cgcttgtctg agattggtta acaggtctct 2040 agggtcattc ccttcataga aaaacaggtc tctttgttgc tctaaacagg gcttataggt 2100 attcagaatg gtgaatggtt tgaactcaca accgtcaaga atgtacttga ttagctcata 2160 ggagatggga gataaatcag catcagagca gtagagtttt ccgcctgtcc ttagggcata 2220 aaggattagg ttgtagaacg tttcaaggat tttcgcccgg tcttgggtac aggtcgagct 2280 acccaaagcg tgccctaaaa cttgctcgat ttcgtctagg acaatgtgag cgccgtgcca 2340 ttccatgaaa ttaaaatgag cttggctatc gggatgtagg ctatcgatac acatcgccat 2400 ccctagagcg cccccggtcg ggctactgtc gagctcgcta atgtgatcga tatcgagacg 2460 gcgggcgagc tccttggcta attggatgcg gtgagtcaaa accagtacgc gagcgcaatc 2520 atcttgggcg agcttaacga ttttccctaa ccattctgtt ttcccggtgc ccttggcaga 2580 tttaatgaaa ataatctgag catccttggg gatagcacgc ggatcgatgt cgagatagcg 2640 ctgatcgacc gttagatcga cttgcgtggt gatatcggtg agattatcca tctcccagac 2700 tgctaagggc ttgcggtgct tgtagcggtt atggagtgct ttcgctccgt gctcgacgat 2760 cagatcatca acacctttcg cgtcctggtg ccaggtgatc accgatactt tcgccccggc 2820 cttctctagg agtgccccgg tagtttggat cgccccgttc actgccttga tcgtcttggg 2880 tttcttgtct tggtcaaaac agaaggcgat cgcccggtta tttttcgcca acaggtcaag 2940 ctctggaatg aggtgccgta gctgtcgcat tggctcgcca tggtcatttt ttggcgttct 3000 gtagccgttg taaatccccg gtagaccgat ggcgcaataa ccagcggtta ggagggcccc 3060 tgccttcttc gcgccctcag tgatgatgat cggtagctca ggatgggcta gtacccattc 3120 ccagaagcct tgatcggttt ctggtagctc gactttgtgg cgcttggcaa tcttgcgcca 3180 ggtgccccgg tctaccttga gagtgaaaat ttcggtggct acccgtggcg ggtgctcata 3240 cttgatcggt ttcttatctt cgcgatcttt acgggggcga tcgcctttga agcaacccca 3300 aagagaatct ttcccggtct taatatcgat gcctgagcac caccaaccgc catgctcgac 3360 aaaggcatat cgcttaagcc aagtatcccg aagcctgcca tcattgcggc gtaatgctgg 3420 ctcatcactg tagagcaggt attcataggg cgtcatccct gacaacgatc taacatttaa 3480 ccgggcaatc tcaggggcga ccccggaacc ctctacccat tccctatagt gagattctgc 3540 gataaaatga gaacggttct cactgacgct actcgctaaa tctgccccgg tattactgct 3600 agatgtaaaa ttatctgata cgatcacagt gagttaagga gcttggcgta atcatggtca 3660 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 3720 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 3780 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 3840 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 3900 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3960 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4020 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4080 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 4140 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 4200 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 4260 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4320 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4380 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4440 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 4500 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4560 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4620 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4680 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4740 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4800 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4860 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4920 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4980 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 5040 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 5100 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 5160 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 5220 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 5280 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 5340 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 5400 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 5460 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 5520 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 5580 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 5640 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 5700 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 5760 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 5820 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc 5878  pAQJ4-MCS"Times New Roman pNdeI(185),PvuII(309),XbaI(402),PstI(412),BamHI(414),XmaI(418),SmaI(420),KpnI(426),ClaI(429),EcoRI(434),SacI(444) Arial Y1//.j #HindIII(806),EcoRV(1021),HpaI(1178) Arial.)$$ 7ClaI(1374),SacI(1563),HpaI(1708),EcoRV(1762),ClaI(1764) Arial "ClaI(1887),HpaI(2030),Bsu36I(2211) Arialw %%I NClaI(2385),SacI(2436),ClaI(2448),EcoRV(2452),SacI(2472),ClaI(2625),EcoRV(2674) Arial ,a1i6q6qv  BclI(2797),NcoI(2979),ApaI(3059) Arial  NruI(3263),ClaI(3327) Arial nsma,a PvuII Arial!',&2 ScaI ArialB=77CɆ3 pGEX_3_primer"Times New RomanClM13_pUC_fwd_primer"Times New RomanC{M13_forward20_primer"Times New Romanq orf943"Times New RomanCEWM13_reverse_primer"Times New RomanCVlM13_pUC_rev_primer"Times New Romanz lac_promoter"Times New RomanC.ORF 2"Times New RomanCɆ. Ampicillin"Times New Roman(CɆXt AmpR_promoter"Times New Roman test"Times New Roman:zS test"Times New Romanj<41,1 B]