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    Plasmid map of pAS404

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    pAS404
    Gene/insert name: none
    Insert size (bp): Unknown
    Fusion proteins or tags: HA
    Terminal: N terminal on backbone
    Vector backbone: pAS404 (Search Vector Database)
    Type of vector: Yeast expression
    Backbone size (bp): 5465
    Cloning site 5': See map
    Site destroyed during cloning: No
    Cloning site 3': See map
    Site destroyed during cloning: No
    5' Sequencing primer: Gal4-Nterm (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Selectable markers: TRP1
    Plasmid Provided In: DH5a
    Principal Investigator: Eishi Noguchi
    Comments: S. cerevisiae-E. coli shuttle vector. Cloning vector for two-hybrid system to express a GAL4 DBD-HA hybrid protein under the control of the ADH1 promoter. The 1.2-kb SacI/SalI fragment of pAS1 containing the ADH promoter, the GAL4 DNA-binding domain, and the HA epitope was inserted into the SacI/SalI sites of pRS404. Multi cloning sites from pBluescript II, f1(+) from pBluescript II.
    Article: Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p. Nakashima N et al. (Genetics. 1999 Jul . 152(3):853-67. Pubmed)
    Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.



    image of pAS404



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