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pAS404
Gene/insert name: none
Insert size (bp): Unknown
Fusion proteins or tags: HA
Terminal: N terminal on backbone
Vector backbone: pAS404 (Search Vector Database)
Type of vector: Yeast expression
Backbone size (bp): 5465
Cloning site 5': See map
Site destroyed during cloning: No
Cloning site 3': See map
Site destroyed during cloning: No
5' Sequencing primer: Gal4-Nterm (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: TRP1
Plasmid Provided In: DH5a
Principal Investigator: Eishi Noguchi
Comments: S. cerevisiae-E. coli shuttle vector. Cloning vector for two-hybrid system to express a GAL4 DBD-HA hybrid protein under the control of the ADH1 promoter. The 1.2-kb SacI/SalI fragment of pAS1 containing the ADH promoter, the GAL4 DNA-binding domain, and the HA epitope was inserted into the SacI/SalI sites of pRS404. Multi cloning sites from pBluescript II, f1(+) from pBluescript II.
Article: Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p. Nakashima N et al. (Genetics. 1999 Jul . 152(3):853-67. Pubmed)
Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.