Sequence of pAS404

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Sequence of pAS404

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Sequence of pAS404 : 5465 BP; 1452 A; 1350 C; 1191 G; 1472 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 60

1 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 60

.......... .......... .......... .......... .......... ..........

AatII(69) .......... .......... .......... .......... ..........

61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 120

61 gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 180

121 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 180

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 240

181 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 240

.......... .......... ....... ORF3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 300

241 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 300

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 360

301 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 360

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 420

361 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 420

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 480

421 atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg 480

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 540

481 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 540

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 600

541 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 600

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 660

601 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 660

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 720

661 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 720

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 780

721 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 780

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 840

781 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 840

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 900

841 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 900

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 960

901 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 960

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1020

961 gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt gctttatctg 1020

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1080

1021 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 1080

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1140

1081 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1200

1141 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1260

1201 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 1260

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1320

1261 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 1380

1321 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacagg 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1440

1381 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1500

1441 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1560

1501 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1620

1561 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1680

1621 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1740

1681 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1800

1741 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1860

1801 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 1860

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1920

1861 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1980

1921 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2040

1981 gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2100

2041 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc 2160

2101 tgcgttaatt acactcaatg gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 2220

2161 gccgaggata caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac 2220

.......... .......... .......... M13_pUC_r .......... M13_r

.......... .......... .......... .......... .......... ..........

2221 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcg 2280

2221 tggtactaat gcggttcgcg cgttaattgg gagtgatttc ccttgttttc gacctcgagc 2280

.......... .......... .......... T3_pro .......... ..........

BglII(2281) ........ .......... .......... .......... ..........

2281 agatctagat atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca 2340

2281 tctagatcta taggaaaaca acaaaggccc acatgttata cctgaaggag aaaagaccgt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg 2400

2341 tggtttgggt atgtagccct aaggatatta tggaagcaac cagagggatt gtacatccac 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac 2460

2401 cgcctcccct ctatatgtta tcttgtctat ggtctgttct gtattacccg atttgttctg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact gtagccctag 2520

2461 atgtggttaa tgtgacggag taactaccac catgtattgc ttgattatga catcgggatc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc catctattga 2580

2521 tgaactatcg gtagtagtat agcttcaaag tgatgggaaa aaggtaaacg gtagataact 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt 2640

2581 tcattattat ccgcgtacgt tgaagaaaag aaaaaaaaag aaaagagaga gggggcaaca 2640

.......... .......... .......... yADH1_pro .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tgtctcacca tatccgcaat gacaaaaaaa atgatggaag acactaaagg aaaaaattaa 2700

2641 acagagtggt ataggcgtta ctgttttttt tactaccttc tgtgatttcc ttttttaatt 2700

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... ......... BstBI(2755)

2701 cgacaaagac agcaccaaca gatgtcgttg ttccagagct gatgaggggt atcttcgaac 2760

2701 gctgtttctg tcgtggttgt ctacagcaac aaggtctcga ctactcccca tagaagcttg 2760

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acacgaaact ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac 2820

2761 tgtgctttga aaaaggaagg aagtaagtgc gtgtgatgag agattactcg ttgccatatg 2820

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggccttcctt ccagttactt gaatttgaaa taaaaaaagt ttgccgcttt gctatcaagt 2880

2821 ccggaaggaa ggtcaatgaa cttaaacttt atttttttca aacggcgaaa cgatagttca 2880

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct 2940

2881 tatttatctg gacgttaata attagaaaac aaaggagcag taacaagagc aagggaaaga 2940

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tccttgtttc tttttctgca caatatttca agctatacca agcatacaat caactccaag 3000

2941 aggaacaaag aaaaagacgt gttataaagt tcgatatggt tcgtatgtta gttgaggttc 3000

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttgaagcaa gcctcctgaa agatgaagct actgtcttct atcgaacaag catgcgatat 3060

3001 gaacttcgtt cggaggactt tctacttcga tgacagaaga tagcttgttc gtacgctata 3060

.......... .......... .......... ........ Gal4_DBD ORF2 ........

.......... .......... .......... .......... .......... ..........

3061 ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa 3120

3061 aacggctgaa tttttcgagt tcacgaggtt tctttttggc ttcacgcggt tcacagactt 3120

.......... ........ Gal4_DBD ORF2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca 3180

3121 cttgttgacc ctcacagcga tgagagggtt ttggttttcc agaggcgact gatcccgtgt 3180

.......... ........ Gal4_DBD ORF2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc 3240

3181 agactgtctt caccttagtt ccgatctttc tgaccttgtc gataaagatg actaaaaagg 3240

.......... ........ Gal4_DBD ORF2 ........ .......... ..........

.......... .......... .......... .......... .......... HpaI(3300)

3241 tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt 3300

3241 agctcttctg gaactgtact aaaactttta cctaagaaat gtcctatatt ttcgtaacaa 3300

.......... ........ Gal4_DBD ORF2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc 3360

3301 ttgtcctaat aaacatgttc tattacactt atttctacgg cagtgtctat ctaaccgaag 3360

.......... ........ Gal4_DBD ORF2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agtggagact gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc 3420

3361 tcacctctga ctatacggag attgtaactc tgtcgtatct tattcacgct gtagtagtag 3420

.......... ........ Gal4_DBD ORF2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggaagagagt agtaacaaag gtcaaagaca gttgactgta tcgccggaat tcatggctta 3480

3421 ccttctctca tcattgtttc cagtttctgt caactgacat agcggcctta agtaccgaat 3480

..... Gal4_N_term_pri Gal4_DBD ORF2 ..... .......... ..........

.......... ........ NheI NdeI(3519),MscI(3524),NcoI(3525) BamHI(3540)

3481 cccatacgat gttccagatt acgctagctt gggtggtcat atggccatgg aggccccggg 3540

3481 gggtatgcta caaggtctaa tgcgatcgaa cccaccagta taccggtacc tccggggccc 3540

.......... HA HA ORF2 .......... .......... ..........

SalI) SalI(3546) ApaI(3565) ApaI(3565) KpnI(3571) ......

3541 gatccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac 3600

3541 ctaggcagct ggagctcccc cccgggccat gggttaagcg ggatatcact cagcataatg 3600

.......... .......... ORF2 .......... T7_pro ..........

.......... .......... .......... .......... .......... ..........

3601 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 3660

3601 cgcgcgagtg accggcagca aaatgttgca gcactgaccc ttttgggacc gcaatgggtt 3660

..... M13_f20_pri ORF2 M13_pUC_f_pri lacZ_a ..... ..........

.......... .......... .......... .......... .......... ..........

3661 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 3720

3661 gaattagcgg aacgtcgtgt agggggaaag cggtcgaccg cattatcgct tctccgggcg 3720

.......... ......... ORF2 lacZ_a ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt 3780

3721 tggctagcgg gaagggttgt caacgcgtcg gacttaccgc ttaccgcgct gcgcgggaca 3780

.......... ORF2 lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 3840

3781 tcgccgcgta attcgcgccg cccacaccac caatgcgcgt cgcactggcg atgtgaacgg 3840

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 3900

3841 tcgcgggatc gcgggcgagg aaagcgaaag aagggaagga aagagcggtg caagcggccg 3900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 3960

3901 aaaggggcag ttcgagattt agcccccgag ggaaatccca aggctaaatc acgaaatgcc 3960

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 4020

3961 gtggagctgg ggttttttga actaatccca ctaccaagtg catcacccgg tagcgggact 4020

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 4080

4021 atctgccaaa aagcgggaaa ctgcaacctc aggtgcaaga aattatcacc tgagaacaag 4080

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 4140

4081 gtttgacctt gttgtgagtt gggatagagc cagataagaa aactaaatat tccctaaaac 4140

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 4200

4141 ggctaaagcc ggataaccaa ttttttactc gactaaattg tttttaaatt gcgcttaaaa 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg 4260

4201 ttgttttata attgcaaatg ttaaaggact acgccataaa agaggaatgc gtagacacgc 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtatttcaca ccgcataggc aagtgcacaa acaatactta aataaatact actcagtaat 4320

4261 cataaagtgt ggcgtatccg ttcacgtgtt tgttatgaat ttatttatga tgagtcatta 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt 4380

4321 ttggataaag aatcgtaaaa actgctttaa acgataaaac aatctcagaa aatgtggtaa 4380

.......... .......... .......... ORF1 TRP1 .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc 4440

4381 acagaggtgt ggaggcgaat gtagttgtgg ttattgcggt aaattagatt cgcgtagtgg 4440

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg ggctctcttg 4500

4441 ttgtaaaaga ccgcagtcag gtggtcgatt gtattttaca ttcgaaagcc ccgagagaac 4500

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc 4560

4501 ggaaggttgg gtcagtcttt agctcaaggt taggttttca agtggacagg gtggacgaag 4560

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt 4620

4561 acttagtttg ttcccttatt tgcttactcc aaagacactt cgacgtgact catcatacaa 4620

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc 4680

4621 cgtcagaaaa cctttatgct cagaaaatta ttgaccgttt ggctccttga gaaccataag 4680

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc 4740

4681 aacggtgctg agtagaggta cgtcaacctg ctatagttac ggcattagta actggtctcg 4740

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga 4800

4741 gttttgtagg aggaatccaa ctaatgcttt gtgcggttgg ttcataaagc ctcacggact 4800

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 actattttta tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt 4860

4801 tgataaaaat atacgaaaat gttctgaact ttaaaaggaa cgttattggc ccagttaaca 4860

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat ctagagcaca 4920

4861 agagaaagat aacccgtgtg tatattatgg gtcgttcagt cgtagcctta gatctcgtgt 4920

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt 4980

4921 aagacgccgg agacacgaga cgttcggcgt ttgaaagtgg ttacctggtc ttgatggaca 4980

.......... .......... ORF1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... PmlI(5038)

4981 gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt 5040

4981 ctttaattat tgtctgtatg aggttcgacg gaaacacacg aattagtgca tatgagtgca 5040

ORF1 TRP1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt 5100

5041 cgagttatca gtggttacgg gagggagaac cgggagagga aaagaaaaaa gctggcttaa 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa ggtgacaagc 5160

5101 ttaagaatta gccgtttttt tcttttcgag gcctagttct aacatgcatt ccactgttcg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact gcaaagtaca 5220

5161 ataaaaagtt atttcttata gaaggtgatg acggtagacc gcagtattga cgtttcatgt 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 catatattac gatgctgtct attaaatgct tcctatatta tatatatagt aatgtcgttt 5280

5221 gtatataatg ctacgacaga taatttacga aggatataat atatatatca ttacagcaaa 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc 5340

5281 taccacgtga gagtcatgtt agacgagact acggcgtatc aattcggtcg gggctgtggg 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 5400

5341 cggttgtggg cgactgcgcg ggactgcccg aacagacgag ggccgtaggc gaatgtctgt 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 5460

5401 tcgacactgg cagaggccct cgacgtacac agtctccaaa agtggcagta gtggctttgc 5460

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgcga..... .......... .......... .......... .......... .......... 5465

5461 gcgct..... .......... .......... .......... .......... .......... 5465

.......... .......... .......... .......... .......... ..........