Sequence of pAW8

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Sequence of pAW8_1XFLAG

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Sequence of pAW8_1XFLAG: 9953 BP; 2800 A; 2134 C; 2080 G; 2939 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 60

1 gtttttcaat ccacattgtc ttttagtact ttgatactaa agattaaata tataacctcc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 120

61 taaaagagat tttttttttt atgttgttta ttttttgtga gttactggac tggtaaacta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 180

121 cctcaaattc agttatggaa gaacttggta aagggtatta ccactttcaa gggagttctt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 240

181 aaaatgagac agtctttgcc ggaattgctg catcagctag gcctgctacc ggttggaagg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 300

241 ttaagtaatt tagctcagga ggtagtctac taaatgttcc aaaacgtatc ttagggattc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 360

301 attctcttca acaatcacta aagaagttca aatgatttta aatgtatgtt tacctactag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 420

361 agaagaggta acaaaggaaa gaagaacgtt tgcgcaaaat gtttctctac tgtacgataa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 480

421 aagactaata aaaaaagata tcaaaagata aaacatgcag gaaccctcag gctttacatt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 540

481 ttagccccat accaatcatc gccgtcgcaa gtaataccat atattttcta tttttcatcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 600

541 ccgttcgctc tgtcttaccc tatgttcccg tagcagatat taatatcgat ttttaacata 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 660

601 aaattaaaca taaaaaacat taaaataaag agtaacaaaa tgaattttac ttacgcttaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 720

661 tctcttttga agttgcttta cagttttatt cgagtcgttt tatatgttaa aatccctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 780

721 gctagtcgtt gaataagagg ccacatctaa gaaaaagaag tatttaaggt tgttacattg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 840

781 acggttaact aaaaagagat ctacgattat ttaactttga ttacttatat tattgtgtcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 900

841 aaaactattt aacatttgag aatgagtttt tttcttacaa ttttgattaa attggaaaaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 960

901 tatttccgag taccgcaatt tctcctatca tttttaagat gttaaaaaat taaattacag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ScaI(1014)

961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1020

961 tgttaacaga acgcttatat taagataatt aaaagcgatt gacgacaaaa tcatgataat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1080

1021 gaaagttata cggaatatac gttgaaatta agtacttctt acgttataaa aacgttagaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1140

1081 agaacatgac tcgcgaaaaa tagtaaatca tgagtaataa attaaaaagt catttcccgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1200

1141 tatttattta aacatcttct acgttacatt agaggagata ggaaaacgag tatacaaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1260

1201 ctcatatgga tcagatcttt ccgaacataa attttataat aagttatttt taagtgttaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1320

1261 aaatgctgta cacgatggta agtgaattga aggactaata ttttaaccaa gcaaatatga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1380

1321 ttaatgaatt catggaaatt gatttgtttt acggatatat atataattaa atgttactca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(1429) ..........

1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1440

1381 cagtctattc agtgatacag gctcaccaat tcctcaatct gagcggtagc taccatacca 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1500

1441 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct gtgggcggtt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1560

1501 gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg tctgttcgac 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1620

1561 actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct 1620

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1680

1621 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1740

1681 gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1800

1741 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1800

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1860

1801 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1860

.......... .......... ....... Ampicillin ORF 2 ....... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1920

1861 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1920

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1980

1921 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1980

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2040

1981 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2040

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2100

2041 atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg 2100

Ampicillin .......... ORF 2 .......... .......... ..........

.......... .......... ScaI(2128) .......... .......... ..........

2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2160

2101 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2220

2161 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2280

2221 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2340

2281 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2400

2341 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 2400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2460

2401 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2520

2461 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2520

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2580

2521 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2580

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2640

2581 gggcatagca tcaatagatg tgctgcccct cagtccggtg atacctactt gctttatctg 2640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2700

2641 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2760

2701 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2820

2761 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2880

2821 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2880

.......... .......... .......... pBR322_ori .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2940

2881 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2940

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3000

2941 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacaag 3000

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3060

3001 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 3060

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3120

3061 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 3120

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3180

3121 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 3180

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3240

3181 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 3240

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3300

3241 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 3300

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3360

3301 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 3360

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3420

3361 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 3420

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3480

3421 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3480

.......... pBR322_ori .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3540

3481 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3600

3541 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3660

3601 gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3720

3661 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3780

3721 tgcgttaatt acactcaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 3780

.......... .......... .......... .......... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3840

3781 gccgagcata caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac 3840

lac_promoter ........ ...... M13_pUC_rev_primer .. M13_reverse_primer

........ HindIII(3856) ........ .......... .......... ..........

3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3900

3841 tggtactaat gcggttcgaa cgtaccgccg gcgtattgaa gcatatcgta tgtaatatgc 3900

.......... .......... .......... .......... loxP ..........

.......... SpeI(3917) .......... .......... XbaI(3947) ..........

3901 aagttatgca tgctcactag tgactacaag gacgacgatg acaagtctag atgaggcgcg 3960

3901 ttcaatacgt acgagtgatc actgatgttc ctgctgctac tgttcagatc tactccgcgc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa 4020

3961 ggtgaagatt tattcgctta aagaatacta aatactaaaa ataataattt attcaatatt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat 4080

4021 ttttttattc acatatgttt aaaatttcac tgagaatcca aaattttgct tttaagaata 4080

.......... ...... pHybLex_rev_primer ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat gaggtcgctc 4140

4081 agaactcatt gagaaaggac atccagtcca acgaaagagt ccatatcata ctccagcgag 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... BglII(4193)

4141 ttattgacca cacctctacc ggcagatccg ctagggataa cagggtaata tagatctgtt 4200

4141 aataactggt gtggagatgg ccgtctaggc gatccctatt gtcccattat atctagacaa 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc 4260

4201 atcgaacgga gcaggggcgg cccagtgggc cggtcgctgt acctccgggt cttatgggag 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag 4320

4261 gaactgtcag aactgcacgc gtcgagtccc cgtactacac tgacagcggg catgtaaatc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga 4380

4321 gggtatgtag gggtacatat tagtaaacgt aggtatgtaa aactaccggc gtgccgcgct 4380

.......... .......... .......... .......... .......... ..........

.......... .......... PstI(4407) .......... .......... ..........

4381 agcaaaaatt acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac 4440

4381 tcgtttttaa tgccgaggag cgacgtctgg acgctcgtcc ctttgcgagg ggagtgtctg 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca 4500

4441 cgcaacttaa caggggtgcg gcgcggggac atctctttat attttccaat cctaaacggt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc 4560

4501 gactccaaga agaaagtata tgaaggaaaa ttttagaacg atcctatgtc aagagtgtag 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 acatccgaac ataaacaacc atgggtaagg aaaagactca cgtttcgagg ccgcgattaa 4620

4561 tgtaggcttg tatttgttgg tacccattcc ttttctgagt gcaaagctcc ggcgctaatt 4620

.......... .......... .......... kanR .......... ..........

.......... .......... .......... .......... .......... ..........

4621 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 4680

4621 taaggttgta cctacgacta aatataccca tatttacccg agcgctatta cagcccgtta 4680

.......... .......... kanR .......... .......... ..........

.......... ClaI(4698) .......... .......... .......... ..........

4681 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac 4740

4681 gtccacgctg ttagatagct aacataccct tcgggctacg cggtctcaac aaagactttg 4740

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 4800

4741 taccgtttcc atcgcaacgg ttactacaat gtctactcta ccagtctgat ttgaccgact 4800

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 4860

4801 gccttaaata cggagaaggc tggtagttcg taaaataggc atgaggacta ctacgtacca 4860

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tactcaccac tgcgatcccc ggcaaaacag cattccaggt attagaagaa tatcctgatt 4920

4861 atgagtggtg acgctagggg ccgttttgtc gtaaggtcca taatcttctt ataggactaa 4920

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 4980

4921 gtccactttt ataacaacta cgcgaccgtc acaaggacgc ggccaacgta agctaaggac 4980

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 5040

4981 aaacattaac aggaaaattg tcgctagcgc ataaagcaga gcgagtccgc gttagtgctt 5040

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 5100

5041 acttattgcc aaaccaacta cgctcactaa aactactgct cgcattaccg accggacaac 5100

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc 5160

5101 ttgttcagac ctttctttac gtattcgaaa acggtaagag tggcctaagt cagcagtgag 5160

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 5220

5161 taccactaaa gagtgaacta ttggaataaa aactgctccc ctttaattat ccaacataac 5220

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 5280

5221 tacaacctgc tcagccttag cgtctggcta tggtcctaga acggtaggat accttgacgg 5280

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 5340

5281 agccactcaa aagaggaagt aatgtctttg ccgaaaaagt ttttatacca taactattag 5340

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagtactga 5400

5341 gactatactt atttaacgtc aaagtaaact acgagctact caaaaagatt agtcatgact 5400

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt 5460

5401 gttatttttc taagaacaaa agttcttgaa cagtaaacat atcaaaaaaa tataacatca 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg 5520

5461 acaagataaa attagtttac aatcgcacta aatataaaaa aaagcggagc tgtagtagac 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct 5580

5521 gggtctacgc ttcaattcac gcgtctttca ttatagtacg cagttagcat acacttacga 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... SalI(5625) ..........

5581 ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccagtcgacg aactagtata 5640

5581 ccagcgatat gacgacagct aagctatgat tgcggcggta ggtcagctgc ttgatcatat 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .... NotI(5674),EagI(5674) .... ..........

5641 acttcgtata tggtattata tacgaagtta tgcggccgct gaattcactg gccgtcgttt 5700

5641 tgaagcatat accataatat atgcttcaat acgccggcga cttaagtgac cggcagcaaa 5700

.......... .......... .......... .......... ..... M13_forward20_primer

.......... .......... .......... .......... .......... ..........

5701 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 5760

5701 atgttgcagc actgaccctt ttgggaccgc aatgggttga attagcggaa cgtcgtgtag 5760

M13_pUC_fwd_primer .. .......... .......... .......... ..........

......... PvuII(5774) ......... .......... .......... ..........

5761 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 5820

5761 ggggaaagcg gtcgaccgca ttatcgcttc tccgggcgtg gctagcggga agggttgtca 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg 5880

5821 acgcgtcgga cttaccgctt accgcggact acgccataaa agaggaatgc gtagacacgc 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gtatttcaca ccgcatacca tcgtccggat caatgaagta gatgagttta ctacctgtat 5940

5881 cataaagtgt ggcgtatggt agcaggccta gttacttcat ctactcaaat gatggacata 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 atactttttt ttttgtcaaa gtcactttta tggcatttcc atgttgaagg attagccaca 6000

5941 tatgaaaaaa aaaacagttt cagtgaaaat accgtaaagg tacaacttcc taatcggtgt 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gcactaaatt ttcagcgtat gattgctttt aaatatttaa ttttcatcgt tttttaatta 6060

6001 cgtgatttaa aagtcgcata ctaacgaaaa tttataaatt aaaagtagca aaaaattaat 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 atattcaaaa cgatttaatg ccttctacat ctgaagtcaa gctcatagac tcgggttggg 6120

6061 tataagtttt gctaaattac ggaagatgta gacttcagtt cgagtatctg agcccaaccc 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tagctaaaac tacatctaac acgaaggggt atagccttct taaagtatac tcatgtataa 6180

6121 atcgattttg atgtagattg tgcttcccca tatcggaaga atttcatatg agtacatatt 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 acgttttggg ttcatatttt tgggccacgt ggtttataaa aattcttaac tacaccactc 6240

6181 tgcaaaaccc aagtataaaa acccggtgca ccaaatattt ttaagaattg atgtggtgag 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ggtatcccgc acccgtctac gtttctacga tttcgagcta atattgagta aagtgattag 6300

6241 ccatagggcg tgggcagatg caaagatgct aaagctcgat tataactcat ttcactaatc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 caaaaaaaat aaaagtactc gttgtcggag atcaagaatt tttctattat ctcatctaaa 6360

6301 gtttttttta ttttcatgag caacagcctc tagttcttaa aaagataata gagtagattt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ccactttcta aaagcgaaaa acaaaatcgt aatatgcagc ttgaatgggc ttccatagtt 6420

6361 ggtgaaagat tttcgctttt tgttttagca ttatacgtcg aacttacccg aaggtatcaa 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tgaaagaaaa accctagcag tactggcaag ggagacattc cttttacctg gctaatcgcc 6480

6421 actttctttt tgggatcgtc atgaccgttc cctctgtaag gaaaatggac cgattagcgg 6480

.......... .......... .......... .......... .......... ORF 1

.......... .......... .......... .......... .......... ..........

6481 atcttccagc aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat 6540

6481 tagaaggtcg tccgcgtggt aacggggaca aagtgatagg tccaatgcct atatcaagta 6540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gacaatattt acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc 6600

6541 ctgttataaa tgtaaccagg tcggtggtcg aacgtactag aggccataac tttgaggtcg 6600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct 6660

6601 cgcccggtat agagcgcgcc gaggctgtgc ccgtgacaca ggtctggtcc ggtccataga 6660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... ClaI(6695) .......... ..........

6661 ctgaccagag tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc 6720

6661 gactggtctc agtaggaatc gcggcattta gttagctact caacgaagtt tttagggaag 6720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cagggcgcga gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac 6780

6721 gtcccgcgct caactatcga ccgaccaccg tctaccgcgc cgttgtggta aaaaagactg 6780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ccggcaaaac aggtagttat tcggatcatc agctacacca gagacggaaa tccatcgctc 6840

6781 ggccgttttg tccatcaata agcctagtag tcgatgtggt ctctgccttt aggtagcgag 6840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gaccagttta gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt 6900

6841 ctggtcaaat caatgggggt ccgattcacg gaagagatgt ggacgccacg attggtcgca 6900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tttcgttctg ccaatatgga ttaacattct cccaccgtca gtacgtgaga tatctttaac 6960

6901 aaagcaagac ggttatacct aattgtaaga gggtggcagt catgcactct atagaaattg 6960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 cctgatcctg gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc 7020

6961 ggactaggac cgttaaagcc gatatgcatt gtcccacaat attcgttagg ggtctttacg 7020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cagattacgt atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa 7080

7021 gtctaatgca tataggaccg tcgctagcga taaaaggtac tcacttgctt ggaccagctt 7080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 atcagtgcgt tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt 7140

7081 tagtcacgca agcttgcgat ctcggacaaa acgtgcaagt ggccgtagtt gcaaaagaaa 7140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tcggatccgc cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga 7200

7141 agcctaggcg gcgtattggt cactttgtcg taacgacagt gaaccagcac cgtcgggcct 7200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ccgacgatga agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc 7260

7201 ggctgctact tcgtacaaat cgaccgggtt tacaacgacc tatcaaaaat gacggtctgg 7260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 gcgcgcctga agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt 7320

7261 cgcgcggact tctatatctt ctattagcgc ttgtagaagt ccaagacgcc ctttggtaaa 7320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ccggttattc aacttgcacc atgccgccca cgaccggcaa acggacagaa gcattttcca 7380

7321 ggccaataag ttgaacgtgg tacggcgggt gctggccgtt tgcctgtctt cgtaaaaggt 7380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 ggtatgctca gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc 7440

7381 ccatacgagt cttttgcgga ccgctaggga cttgtacagg tagtccaaga acgcttggag 7440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 atcactcgtt gcatcgaccg gtaactttta gattaacatt tgtttaaata tgaaattagg 7500

7441 tagtgagcaa cgtagctggc cattgaaaat ctaattgtaa acaaatttat actttaatcc 7500

.......... .......... .......... .......... .......... ..........

.......... PstI(7518) .......... .......... .......... NdeI(7552)

7501 caatgtaaca tactgcaggc aaattttggt gtacggtaag taaattggac atatgattta 7560

7501 gttacattgt atgacgtccg tttaaaacca catgccattc atttaacctg tatactaaat 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 acaaagcgac tataagtcag aaagtgagaa tgagattgaa ataattaatt caacttattc 7620

7561 tgtttcgctg atattcagtc tttcactctt actctaactt tattaattaa gttgaataag 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 aattgatgat atgccaggat tcctcttcct ttatatatct tcaatgtttc gattaacagg 7680

7621 ttaactacta tacggtccta aggagaagga aatatataga agttacaaag ctaattgtcc 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tccattatcc ggtttttagt ttcgctgcac attgccgaat gacaatcgga ttcctttaag 7740

7681 aggtaatagg ccaaaaatca aagcgacgtg taacggctta ctgttagcct aaggaaattc 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 ctttgctttt gccctgtttt ctctccccag taatcgttgg gtgactgaac catccagtag 7800

7741 gaaacgaaaa cgggacaaaa gagaggggtc attagcaacc cactgacttg gtaggtcatc 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ccgagagtta cgtttttcct gatttaataa atatccatat ctatttgtct cgtggtgttt 7860

7801 ggctctcaat gcaaaaagga ctaaattatt tataggtata gataaacaga gcaccacaaa 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 tacagcaact aaccgaaatt actattttgc atcatccaac cataaagaga caacaataat 7920

7861 atgtcgttga ttggctttaa tgataaaacg tagtaggttg gtatttctct gttgttatta 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 tctagaaaac atataacaag tgtttttttc tatttacgtc tcatacgcaa agtccacttt 7980

7921 agatcttttg tatattgttc acaaaaaaag ataaatgcag agtatgcgtt tcaggtgaaa 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 accatgtttt ggaaacttgt acccaaccat tcatttttcg attttgtaat gaggctcttt 8040

7981 tggtacaaaa cctttgaaca tgggttggta agtaaaaagc taaaacatta ctccgagaaa 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 tcttctaaca atcattgagg tcattatcat gtgtcctcac ctattgttta aattgaggct 8100

8041 agaagattgt tagtaactcc agtaatagta cacaggagtg gataacaaat ttaactccga 8100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 tgcgctattc ccaaaattgt gggtattatt ttcatatatc agtatctgca atttcggttc 8160

8101 acgcgataag ggttttaaca cccataataa aagtatatag tcatagacgt taaagccaag 8160

.......... .......... .......... .......... .......... ..........

.......... KpnI(8180) .......... .......... .......... ..........

8161 aactccggaa gctcggtacc caactgtggg aatactcagg tatcgtaaga tgcaagagtt 8220

8161 ttgaggcctt cgagccatgg gttgacaccc ttatgagtcc atagcattct acgttctcaa 8220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 cgaatctctt agcaaccatt attttttttc tctcaacata acgagaacac acaggggcgc 8280

8221 gcttagagaa tcgttggtaa taaaaaaaag agagttgtat tgctcttgtg tgtccccgcg 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 tatcgcacag aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga 8340

8281 atagcgtgtc ttagtttaag ctactgacct ttaaaaaaca attaaagtct ccagcggact 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 cgcatatacc tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac 8400

8341 gcgtatatgg aaaaagttga ctttttaacc ctctttttcc tttccactct cgcggccttg 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga 8460

8401 gccgaaaagt atatcttatc tcttcgcaag tactgattta cgaacgtagt gttatgaact 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 agttgacaat attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt 8520

8461 tcaactgtta taataaattc ctggataaca aaaaaggtta tccaccaatc gttagcagaa 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 actttctaac ttttcttacc ttttacattt cagcaatata tatatatata tatatatata 8580

8521 tgaaagattg aaaagaatgg aaaatgtaaa gtcgttatat atatatatat atatatatat 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 tttcaaggat ataccattgt aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac 8640

8581 aaagttccta tatggtaaca ttacagacgg ggattcttct agcagcaaaa cggtccactg 8640

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

8641 cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt 8700

8641 gtgcaaccag ttctttagtg tcggcttcgg taattccaag aatttcgata aagactacaa 8700

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct 8760

8701 gcaaggttac agttcaagct aaagctttta gtaaattaac caccacgacg atagctacga 8760

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 acaggtgttc cacttccaga tgaggcgctg gaagcctcca agaaggctga tgccgttttg 8820

8761 tgtccacaag gtgaaggtct actccgcgac cttcggaggt tcttccgact acggcaaaac 8820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga acaaggttta 8880

8821 aatccacgac acccaccagg atttacccca tggccatcac aatctggact tgttccaaat 8880

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc 8940

8881 gatttttagg catttcttga agttaacatg cggttgaatt ctggtacatt gaaacgtagg 8940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt 9000

8941 ctgagagaaa atctgaatag aggttagttc ggtgttaaac gatttccatg actgaagcaa 9000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 gttgttagag aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat 9060

9001 caacaatctc ttaatcaccc tccataaatg aaaccattct ctttccttct gctaccacta 9060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg 9120

9061 ccacagcgaa ccctatcact tgttatgtgg caaggtcttc acgtttctta gtgttcttac 9120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct 9180

9121 cggcgaaagt accgggatgt tgtactcggt ggtaacggat aaaccaggaa cctatttcga 9180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa 9240

9181 ttacaaaacc ggagaagttc taatacctct ttttgacacc tcctttggta gttcttgctt 9240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 tttcctacat tgaaagttca acatcaattg attgattctg ccgccatgat cctagttaag 9300

9241 aaaggatgta actttcaagt tgtagttaac taactaagac ggcggtacta ggatcaattc 9300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(9352)

9301 aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc 9360

9301 ttgggttggg tggatttacc ataatattag tggtcgttgt acaaaccact atagtagagg 9360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 gatgaagcct ccgttatccc aggctccttg ggtttgttgc catctgcgtc cttggcctct 9420

9361 ctacttcgga ggcaataggg tccgaggaac ccaaacaacg gtagacgcag gaaccggaga 9420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... NcoI(9463) ..........

9421 ttgccagaca agaacaccgc atttggtttg tacgaaccat gccatggttc cgctccagat 9480

9421 aacggtctgt tcttgtggcg taaaccaaac atgcttggta cggtaccaag gcgaggtcta 9480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa 9540

9481 aacggtttct tattccagtt gggatagcgg tgatagaaca gacgacgtta ctacaacttt 9540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 ttgtcattga acttgcctga agaaggtaaa gccattgaag atgcagttaa aaaggttttg 9600

9541 aacagtaact tgaacggact tcttccattt cggtaacttc tacgtcaatt tttccaaaac 9600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat 9660

9601 ctacgtccat agtcttgacc actaaatcca ccaaggttgt catggtggct tcagccacta 9660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttgtgatat 9720

9661 cgacagcggc ttcttcaatt cttttaggaa cgaatttttc taagagaaaa aaacactata 9720

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga 9780

9721 aacatgtatt tgaaatattt actttaagta ttatctttgc tgtgctttaa tgttttacct 9780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 atatgttcat agggtagacg aaactatata cgcaatctac atacatttat caagaaggag 9840

9781 tatacaagta tcccatctgc tttgatatat gcgttagatg tatgtaaata gttcttcctc 9840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 aaaaaggagg atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata 9900

9841 tttttcctcc tacatttcct tatgtccatt cgtttaacta tgattaccga gttgcactat 9900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 aggaaaaaga attgcacttt aacattaata ttgacaagga ggagggcatc aca....... 9953

9901 tcctttttct taacgtgaaa ttgtaattat aactgttcct cctcccgtag tgt....... 9953

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-9953 Cross-reference: DOI; 10.1016/j.gene.2007.09.024. Cross-reference: PUBMED; 18054176. Reference author: Watson A.T., Garcia V., Bone N., Carr A.M., Armstrong J.; Reference title: "Gene tagging and gene replacement using recombinase-mediated cassette Reference title: exchange in Schizosaccharomyces pombe"; Reference location: Gene 407(1-2):63-74(2008).
Reference number: [2] Reference positions: 1-9953 Reference author: Watson A.T.; Reference title: ; Reference location: Submitted (26-JUN-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, Reference location: East Sussex BN1 9RQ, UK