Sequence of pAW8

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Sequence of pAW8_6XFLAG

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Sequence of pAW8_6XFLAG: 10103 BP; 2855 A; 2164 C; 2120 G; 2964 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 60

1 gtttttcaat ccacattgtc ttttagtact ttgatactaa agattaaata tataacctcc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 120

61 taaaagagat tttttttttt atgttgttta ttttttgtga gttactggac tggtaaacta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 180

121 cctcaaattc agttatggaa gaacttggta aagggtatta ccactttcaa gggagttctt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 240

181 aaaatgagac agtctttgcc ggaattgctg catcagctag gcctgctacc ggttggaagg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 300

241 ttaagtaatt tagctcagga ggtagtctac taaatgttcc aaaacgtatc ttagggattc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 360

301 attctcttca acaatcacta aagaagttca aatgatttta aatgtatgtt tacctactag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 420

361 agaagaggta acaaaggaaa gaagaacgtt tgcgcaaaat gtttctctac tgtacgataa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 480

421 aagactaata aaaaaagata tcaaaagata aaacatgcag gaaccctcag gctttacatt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 540

481 ttagccccat accaatcatc gccgtcgcaa gtaataccat atattttcta tttttcatcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 600

541 ccgttcgctc tgtcttaccc tatgttcccg tagcagatat taatatcgat ttttaacata 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 660

601 aaattaaaca taaaaaacat taaaataaag agtaacaaaa tgaattttac ttacgcttaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 720

661 tctcttttga agttgcttta cagttttatt cgagtcgttt tatatgttaa aatccctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 780

721 gctagtcgtt gaataagagg ccacatctaa gaaaaagaag tatttaaggt tgttacattg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 840

781 acggttaact aaaaagagat ctacgattat ttaactttga ttacttatat tattgtgtcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 900

841 aaaactattt aacatttgag aatgagtttt tttcttacaa ttttgattaa attggaaaaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 960

901 tatttccgag taccgcaatt tctcctatca tttttaagat gttaaaaaat taaattacag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ScaI(1014)

961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1020

961 tgttaacaga acgcttatat taagataatt aaaagcgatt gacgacaaaa tcatgataat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1080

1021 gaaagttata cggaatatac gttgaaatta agtacttctt acgttataaa aacgttagaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1140

1081 agaacatgac tcgcgaaaaa tagtaaatca tgagtaataa attaaaaagt catttcccgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1200

1141 tatttattta aacatcttct acgttacatt agaggagata ggaaaacgag tatacaaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1260

1201 ctcatatgga tcagatcttt ccgaacataa attttataat aagttatttt taagtgttaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1320

1261 aaatgctgta cacgatggta agtgaattga aggactaata ttttaaccaa gcaaatatga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1380

1321 ttaatgaatt catggaaatt gatttgtttt acggatatat atataattaa atgttactca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(1429) ..........

1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1440

1381 cagtctattc agtgatacag gctcaccaat tcctcaatct gagcggtagc taccatacca 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1500

1441 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct gtgggcggtt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1560

1501 gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg tctgttcgac 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1620

1561 actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct 1620

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1680

1621 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1740

1681 gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1800

1741 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1800

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1860

1801 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1860

.......... .......... ....... Ampicillin ORF 2 ....... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1920

1861 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1920

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1980

1921 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1980

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2040

1981 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2040

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2100

2041 atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg 2100

Ampicillin .......... ORF 2 .......... .......... ..........

.......... .......... ScaI(2128) .......... .......... ..........

2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2160

2101 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2220

2161 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2280

2221 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2340

2281 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2400

2341 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 2400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2460

2401 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2520

2461 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2520

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2580

2521 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2580

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2640

2581 gggcatagca tcaatagatg tgctgcccct cagtccggtg atacctactt gctttatctg 2640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2700

2641 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2760

2701 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2820

2761 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2880

2821 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2880

.......... .......... .......... pBR322_ori .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2940

2881 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2940

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3000

2941 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacaag 3000

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3060

3001 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 3060

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3120

3061 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 3120

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3180

3121 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 3180

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3240

3181 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 3240

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3300

3241 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 3300

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3360

3301 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 3360

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3420

3361 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 3420

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3480

3421 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3480

.......... pBR322_ori .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3540

3481 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3600

3541 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3660

3601 gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3720

3661 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3780

3721 tgcgttaatt acactcaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 3780

.......... .......... .......... .......... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3840

3781 gccgagcata caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac 3840

lac_promoter ........ ...... M13_pUC_rev_primer .. M13_reverse_primer

........ HindIII(3856) ........ .......... .......... ..........

3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3900

3841 tggtactaat gcggttcgaa cgtaccgccg gcgtattgaa gcatatcgta tgtaatatgc 3900

.......... .......... .......... .......... loxP ..........

.......... .......... .......... .......... .......... ..........

3901 aagttatgca tgctcactag tgactacaag gacgacgatg acaagtctag tgactacaag 3960

3901 ttcaatacgt acgagtgatc actgatgttc ctgctgctac tgttcagatc actgatgttc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gacgacgatg acaagtctag tgactacaag gacgacgatg acaagtctag tgactacaag 4020

3961 ctgctgctac tgttcagatc actgatgttc ctgctgctac tgttcagatc actgatgttc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gacgacgatg acaagtctag tgactacaag gacgacgatg acaagtctag tgactacaag 4080

4021 ctgctgctac tgttcagatc actgatgttc ctgctgctac tgttcagatc actgatgttc 4080

.......... .......... .......... .......... .......... ..........

.......... XbaI(4097) .......... .......... .......... ..........

4081 gacgacgatg acaagtctag atgaggcgcg ccacttctaa ataagcgaat ttcttatgat 4140

4081 ctgctgctac tgttcagatc tactccgcgc ggtgaagatt tattcgctta aagaatacta 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg 4200

4141 aatactaaaa ataataattt attcaatatt ttttttattc acatatgttt aaaatttcac 4200

.......... .......... .......... ...... pHybLex_rev_primer ......

.......... .......... .......... .......... .......... ..........

4201 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt 4260

4201 tgagaatcca aaattttgct tttaagaata agaactcatt gagaaaggac atccagtcca 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg 4320

4261 acgaaagagt ccatatcata ctccagcgag aataactggt gtggagatgg ccgtctaggc 4320

.......... .......... .......... .......... .......... ..........

.......... ......... BglII(4343) ......... .......... ..........

4321 ctagggataa cagggtaata tagatctgtt tagcttgcct cgtccccgcc gggtcacccg 4380

4321 gatccctatt gtcccattat atctagacaa atcgaacgga gcaggggcgg cccagtgggc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg 4440

4381 cggtcgctgt acctccgggt cttatgggag gaactgtcag aactgcacgc gtcgagtccc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca 4500

4441 cgtactacac tgacagcggg catgtaaatc gggtatgtag gggtacatat tagtaaacgt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc 4560

4501 aggtatgtaa aactaccggc gtgccgcgct tcgtttttaa tgccgaggag cgacgtctgg 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg 4620

4561 acgctcgtcc ctttgcgagg ggagtgtctg cgcaacttaa caggggtgcg gcgcggggac 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt 4680

4621 atctctttat attttccaat cctaaacggt gactccaaga agaaagtata tgaaggaaaa 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgggtaagg 4740

4681 ttttagaacg atcctatgtc aagagtgtag tgtaggcttg tatttgttgg tacccattcc 4740

.......... .......... .......... .......... .......... kanR

.......... .......... .......... .......... .......... ..........

4741 aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 4800

4741 ttttctgagt gcaaagctcc ggcgctaatt taaggttgta cctacgacta aatataccca 4800

.......... .......... kanR .......... .......... ..........

.......... NruI(4814) .......... .......... ClaI(4848) ..........

4801 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga 4860

4801 tatttacccg agcgctatta cagcccgtta gtccacgctg ttagatagct aacataccct 4860

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 4920

4861 tcgggctacg cggtctcaac aaagactttg taccgtttcc atcgcaacgg ttactacaat 4920

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 4980

4921 gtctactcta ccagtctgat ttgaccgact gccttaaata cggagaaggc tggtagttcg 4980

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag 5040

4981 taaaataggc atgaggacta ctacgtacca atgagtggtg acgctagggg ccgttttgtc 5040

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 5100

5041 gtaaggtcca taatcttctt ataggactaa gtccactttt ataacaacta cgcgaccgtc 5100

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 5160

5101 acaaggacgc ggccaacgta agctaaggac aaacattaac aggaaaattg tcgctagcgc 5160

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 5220

5161 ataaagcaga gcgagtccgc gttagtgctt acttattgcc aaaccaacta cgctcactaa 5220

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt 5280

5221 aactactgct cgcattaccg accggacaac ttgttcagac ctttctttac gtattcgaaa 5280

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 5340

5281 acggtaagag tggcctaagt cagcagtgag taccactaaa gagtgaacta ttggaataaa 5340

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 5400

5341 aactgctccc ctttaattat ccaacataac tacaacctgc tcagccttag cgtctggcta 5400

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 5460

5401 tggtcctaga acggtaggat accttgacgg agccactcaa aagaggaagt aatgtctttg 5460

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 5520

5461 ccgaaaaagt ttttatacca taactattag gactatactt atttaacgtc aaagtaaact 5520

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgctcgatga gtttttctaa tcagtactga caataaaaag attcttgttt tcaagaactt 5580

5521 acgagctact caaaaagatt agtcatgact gttatttttc taagaacaaa agttcttgaa 5580

kanR .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat 5640

5581 cagtaaacat atcaaaaaaa tataacatca acaagataaa attagtttac aatcgcacta 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt 5700

5641 aatataaaaa aaagcggagc tgtagtagac gggtctacgc ttcaattcac gcgtctttca 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta 5760

5701 ttatagtacg cagttagcat acacttacga ccagcgatat gacgacagct aagctatgat 5760

.......... .......... .......... .......... .......... ..........

.......... SalI(5775) .......... .......... .......... ..........

5761 acgccgccat ccagtcgacg aactagtata acttcgtata tggtattata tacgaagtta 5820

5761 tgcggcggta ggtcagctgc ttgatcatat tgaagcatat accataatat atgcttcaat 5820

.......... .......... .......... .......... .......... ..........

NotI(5824),EagI(5824) ........ .......... .......... ..........

5821 tgcggccgct gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 5880

5821 acgccggcga cttaagtgac cggcagcaaa atgttgcagc actgaccctt ttgggaccgc 5880

.......... . M13_forward20_primer M13_pUC_fwd_primer . ..........

.......... .......... .......... ......... PvuII(5924) .........

5881 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 5940

5881 aatgggttga attagcggaa cgtcgtgtag ggggaaagcg gtcgaccgca ttatcgcttc 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga 6000

5941 tccgggcgtg gctagcggga agggttgtca acgcgtcgga cttaccgctt accgcggact 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacca tcgtccggat 6060

6001 acgccataaa agaggaatgc gtagacacgc cataaagtgt ggcgtatggt agcaggccta 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 caatgaagta gatgagttta ctacctgtat atactttttt ttttgtcaaa gtcactttta 6120

6061 gttacttcat ctactcaaat gatggacata tatgaaaaaa aaaacagttt cagtgaaaat 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tggcatttcc atgttgaagg attagccaca gcactaaatt ttcagcgtat gattgctttt 6180

6121 accgtaaagg tacaacttcc taatcggtgt cgtgatttaa aagtcgcata ctaacgaaaa 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 aaatatttaa ttttcatcgt tttttaatta atattcaaaa cgatttaatg ccttctacat 6240

6181 tttataaatt aaaagtagca aaaaattaat tataagtttt gctaaattac ggaagatgta 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ctgaagtcaa gctcatagac tcgggttggg tagctaaaac tacatctaac acgaaggggt 6300

6241 gacttcagtt cgagtatctg agcccaaccc atcgattttg atgtagattg tgcttcccca 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 atagccttct taaagtatac tcatgtataa acgttttggg ttcatatttt tgggccacgt 6360

6301 tatcggaaga atttcatatg agtacatatt tgcaaaaccc aagtataaaa acccggtgca 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ggtttataaa aattcttaac tacaccactc ggtatcccgc acccgtctac gtttctacga 6420

6361 ccaaatattt ttaagaattg atgtggtgag ccatagggcg tgggcagatg caaagatgct 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tttcgagcta atattgagta aagtgattag caaaaaaaat aaaagtactc gttgtcggag 6480

6421 aaagctcgat tataactcat ttcactaatc gtttttttta ttttcatgag caacagcctc 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 atcaagaatt tttctattat ctcatctaaa ccactttcta aaagcgaaaa acaaaatcgt 6540

6481 tagttcttaa aaagataata gagtagattt ggtgaaagat tttcgctttt tgttttagca 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aatatgcagc ttgaatgggc ttccatagtt tgaaagaaaa accctagcag tactggcaag 6600

6541 ttatacgtcg aacttacccg aaggtatcaa actttctttt tgggatcgtc atgaccgttc 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ggagacattc cttttacctg gctaatcgcc atcttccagc aggcgcacca ttgcccctgt 6660

6601 cctctgtaag gaaaatggac cgattagcgg tagaaggtcg tccgcgtggt aacggggaca 6660

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

6661 ttcactatcc aggttacgga tatagttcat gacaatattt acattggtcc agccaccagc 6720

6661 aagtgatagg tccaatgcct atatcaagta ctgttataaa tgtaaccagg tcggtggtcg 6720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ttgcatgatc tccggtattg aaactccagc gcgggccata tctcgcgcgg ctccgacacg 6780

6721 aacgtactag aggccataac tttgaggtcg cgcccggtat agagcgcgcc gaggctgtgc 6780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ggcactgtgt ccagaccagg ccaggtatct ctgaccagag tcatccttag cgccgtaaat 6840

6781 ccgtgacaca ggtctggtcc ggtccataga gactggtctc agtaggaatc gcggcattta 6840

.......... .......... ORF 1 .......... .......... ..........

ClaI(6845) .......... .......... .......... .......... ..........

6841 caatcgatga gttgcttcaa aaatcccttc cagggcgcga gttgatagct ggctggtggc 6900

6841 gttagctact caacgaagtt tttagggaag gtcccgcgct caactatcga ccgaccaccg 6900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 agatggcgcg gcaacaccat tttttctgac ccggcaaaac aggtagttat tcggatcatc 6960

6901 tctaccgcgc cgttgtggta aaaaagactg ggccgttttg tccatcaata agcctagtag 6960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 agctacacca gagacggaaa tccatcgctc gaccagttta gttaccccca ggctaagtgc 7020

6961 tcgatgtggt ctctgccttt aggtagcgag ctggtcaaat caatgggggt ccgattcacg 7020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cttctctaca cctgcggtgc taaccagcgt tttcgttctg ccaatatgga ttaacattct 7080

7021 gaagagatgt ggacgccacg attggtcgca aaagcaagac ggttatacct aattgtaaga 7080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cccaccgtca gtacgtgaga tatctttaac cctgatcctg gcaatttcgg ctatacgtaa 7140

7081 gggtggcagt catgcactct atagaaattg ggactaggac cgttaaagcc gatatgcatt 7140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cagggtgtta taagcaatcc ccagaaatgc cagattacgt atatcctggc agcgatcgct 7200

7141 gtcccacaat attcgttagg ggtctttacg gtctaatgca tataggaccg tcgctagcga 7200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 attttccatg agtgaacgaa cctggtcgaa atcagtgcgt tcgaacgcta gagcctgttt 7260

7201 taaaaggtac tcacttgctt ggaccagctt tagtcacgca agcttgcgat ctcggacaaa 7260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tgcacgttca ccggcatcaa cgttttcttt tcggatccgc cgcataacca gtgaaacagc 7320

7261 acgtgcaagt ggccgtagtt gcaaaagaaa agcctaggcg gcgtattggt cactttgtcg 7320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 attgctgtca cttggtcgtg gcagcccgga ccgacgatga agcatgttta gctggcccaa 7380

7321 taacgacagt gaaccagcac cgtcgggcct ggctgctact tcgtacaaat cgaccgggtt 7380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 atgttgctgg atagttttta ctgccagacc gcgcgcctga agatatagaa gataatcgcg 7440

7381 tacaacgacc tatcaaaaat gacggtctgg cgcgcggact tctatatctt ctattagcgc 7440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 aacatcttca ggttctgcgg gaaaccattt ccggttattc aacttgcacc atgccgccca 7500

7441 ttgtagaagt ccaagacgcc ctttggtaaa ggccaataag ttgaacgtgg tacggcgggt 7500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 cgaccggcaa acggacagaa gcattttcca ggtatgctca gaaaacgcct ggcgatccct 7560

7501 gctggccgtt tgcctgtctt cgtaaaaggt ccatacgagt cttttgcgga ccgctaggga 7560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gaacatgtcc atcaggttct tgcgaacctc atcactcgtt gcatcgaccg gtaactttta 7620

7561 cttgtacagg tagtccaaga acgcttggag tagtgagcaa cgtagctggc cattgaaaat 7620

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gattaacatt tgtttaaata tgaaattagg caatgtaaca tactgcaggc aaattttggt 7680

7621 ctaattgtaa acaaatttat actttaatcc gttacattgt atgacgtccg tttaaaacca 7680

.......... .......... .......... .......... .......... ..........

.......... .......... NdeI(7702) .......... .......... ..........

7681 gtacggtaag taaattggac atatgattta acaaagcgac tataagtcag aaagtgagaa 7740

7681 catgccattc atttaacctg tatactaaat tgtttcgctg atattcagtc tttcactctt 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 tgagattgaa ataattaatt caacttattc aattgatgat atgccaggat tcctcttcct 7800

7741 actctaactt tattaattaa gttgaataag ttaactacta tacggtccta aggagaagga 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ttatatatct tcaatgtttc gattaacagg tccattatcc ggtttttagt ttcgctgcac 7860

7801 aatatataga agttacaaag ctaattgtcc aggtaatagg ccaaaaatca aagcgacgtg 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 attgccgaat gacaatcgga ttcctttaag ctttgctttt gccctgtttt ctctccccag 7920

7861 taacggctta ctgttagcct aaggaaattc gaaacgaaaa cgggacaaaa gagaggggtc 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 taatcgttgg gtgactgaac catccagtag ccgagagtta cgtttttcct gatttaataa 7980

7921 attagcaacc cactgacttg gtaggtcatc ggctctcaat gcaaaaagga ctaaattatt 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 atatccatat ctatttgtct cgtggtgttt tacagcaact aaccgaaatt actattttgc 8040

7981 tataggtata gataaacaga gcaccacaaa atgtcgttga ttggctttaa tgataaaacg 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 atcatccaac cataaagaga caacaataat tctagaaaac atataacaag tgtttttttc 8100

8041 tagtaggttg gtatttctct gttgttatta agatcttttg tatattgttc acaaaaaaag 8100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 tatttacgtc tcatacgcaa agtccacttt accatgtttt ggaaacttgt acccaaccat 8160

8101 ataaatgcag agtatgcgtt tcaggtgaaa tggtacaaaa cctttgaaca tgggttggta 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 tcatttttcg attttgtaat gaggctcttt tcttctaaca atcattgagg tcattatcat 8220

8161 agtaaaaagc taaaacatta ctccgagaaa agaagattgt tagtaactcc agtaatagta 8220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 gtgtcctcac ctattgttta aattgaggct tgcgctattc ccaaaattgt gggtattatt 8280

8221 cacaggagtg gataacaaat ttaactccga acgcgataag ggttttaaca cccataataa 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... KpnI(8330) ..........

8281 ttcatatatc agtatctgca atttcggttc aactccggaa gctcggtacc caactgtggg 8340

8281 aagtatatag tcatagacgt taaagccaag ttgaggcctt cgagccatgg gttgacaccc 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 aatactcagg tatcgtaaga tgcaagagtt cgaatctctt agcaaccatt attttttttc 8400

8341 ttatgagtcc atagcattct acgttctcaa gcttagagaa tcgttggtaa taaaaaaaag 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 tctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga 8460

8401 agagttgtat tgctcttgtg tgtccccgcg atagcgtgtc ttagtttaag ctactgacct 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg 8520

8461 ttaaaaaaca attaaagtct ccagcggact gcgtatatgg aaaaagttga ctttttaacc 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 gagaaaaagg aaaggtgaga gcgccggaac cggcttttca tatagaatag agaagcgttc 8580

8521 ctctttttcc tttccactct cgcggccttg gccgaaaagt atatcttatc tcttcgcaag 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 atgactaaat gcttgcatca caatacttga agttgacaat attatttaag gacctattgt 8640

8581 tactgattta cgaacgtagt gttatgaact tcaactgtta taataaattc ctggataaca 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt 8700

8641 aaaaaggtta tccaccaatc gttagcagaa tgaaagattg aaaagaatgg aaaatgtaaa 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 cagcaatata tatatatata tatatatata tttcaaggat ataccattgt aatgtctgcc 8760

8701 gtcgttatat atatatatat atatatatat aaagttccta tatggtaaca ttacagacgg 8760

.......... .......... .......... .......... .......... ORF 1

.......... .......... .......... .......... .......... ..........

8761 cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc 8820

8761 ggattcttct agcagcaaaa cggtccactg gtgcaaccag ttctttagtg tcggcttcgg 8820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat 8880

8821 taattccaag aatttcgata aagactacaa gcaaggttac agttcaagct aaagctttta 8880

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga tgaggcgctg 8940

8881 gtaaattaac caccacgacg atagctacga tgtccacaag gtgaaggtct actccgcgac 8940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt 9000

8941 cttcggaggt tcttccgact acggcaaaac aatccacgac acccaccagg atttacccca 9000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac 9060

9001 tggccatcac aatctggact tgttccaaat gatttttagg catttcttga agttaacatg 9060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag 9120

9061 cggttgaatt ctggtacatt gaaacgtagg ctgagagaaa atctgaatag aggttagttc 9120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 ccacaatttg ctaaaggtac tgacttcgtt gttgttagag aattagtggg aggtatttac 9180

9121 ggtgttaaac gatttccatg actgaagcaa caacaatctc ttaatcaccc tccataaatg 9180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga acaatacacc 9240

9181 aaaccattct ctttccttct gctaccacta ccacagcgaa ccctatcact tgttatgtgg 9240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca acatgagcca 9300

9241 caaggtcttc acgtttctta gtgttcttac cggcgaaagt accgggatgt tgtactcggt 9300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag attatggaga 9360

9301 ggtaacggat aaaccaggaa cctatttcga ttacaaaacc ggagaagttc taatacctct 9360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 aaaactgtgg aggaaaccat caagaacgaa tttcctacat tgaaagttca acatcaattg 9420

9361 ttttgacacc tcctttggta gttcttgctt aaaggatgta actttcaagt tgtagttaac 9420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg tattataatc 9480

9421 taactaagac ggcggtacta ggatcaattc ttgggttggg tggatttacc ataatattag 9480

.......... .......... ORF 1 .......... .......... ..........

.......... ......... EcoRV(9502) ......... .......... ..........

9481 accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc aggctccttg 9540

9481 tggtcgttgt acaaaccact atagtagagg ctacttcgga ggcaataggg tccgaggaac 9540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc atttggtttg 9600

9541 ccaaacaacg gtagacgcag gaaccggaga aacggtctgt tcttgtggcg taaaccaaac 9600

.......... .......... ORF 1 .......... .......... ..........

.......... NcoI(9613) .......... .......... .......... ..........

9601 tacgaaccat gccatggttc cgctccagat ttgccaaaga ataaggtcaa ccctatcgcc 9660

9601 atgcttggta cggtaccaag gcgaggtcta aacggtttct tattccagtt gggatagcgg 9660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga agaaggtaaa 9720

9661 tgatagaaca gacgacgtta ctacaacttt aacagtaact tgaacggact tcttccattt 9720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg tgatttaggt 9780

9721 cggtaacttc tacgtcaatt tttccaaaac ctacgtccat agtcttgacc actaaatcca 9780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt 9840

9781 ccaaggttgt catggtggct tcagccacta cgacagcggc ttcttcaatt cttttaggaa 9840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 gcttaaaaag attctctttt tttgtgatat ttgtacataa actttataaa tgaaattcat 9900

9841 cgaatttttc taagagaaaa aaacactata aacatgtatt tgaaatattt actttaagta 9900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata 9960

9901 ttatctttgc tgtgctttaa tgttttacct tatacaagta tcccatctgc tttgatatat 9960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa 10020

9961 gcgttagatg tatgtaaata gttcttcctc tttttcctcc tacatttcct tatgtccatt 10020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt aacattaata 10080

10021 cgtttaacta tgattaccga gttgcactat tcctttttct taacgtgaaa ttgtaattat 10080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 ttgacaagga ggagggcatc aca....... .......... .......... .......... 10103

10081 aactgttcct cctcccgtag tgt....... .......... .......... .......... 10103

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-10103 Cross-reference: DOI; 10.1016/j.gene.2007.09.024. Cross-reference: PUBMED; 18054176. Reference author: Watson A.T., Garcia V., Bone N., Carr A.M., Armstrong J.; Reference title: "Gene tagging and gene replacement using recombinase-mediated cassette Reference title: exchange in Schizosaccharomyces pombe"; Reference location: Gene 407(1-2):63-74(2008).
Reference number: [2] Reference positions: 1-10103 Reference author: Watson A.T.; Reference title: ; Reference location: Submitted (26-JUN-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, Reference location: East Sussex BN1 9RQ, UK