Sequence of pAW8

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Sequence of pAW8_6XHis

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Sequence of pAW8_6XHis: 9932 BP; 2793 A; 2132 C; 2070 G; 2937 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 60

1 gtttttcaat ccacattgtc ttttagtact ttgatactaa agattaaata tataacctcc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 120

61 taaaagagat tttttttttt atgttgttta ttttttgtga gttactggac tggtaaacta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 180

121 cctcaaattc agttatggaa gaacttggta aagggtatta ccactttcaa gggagttctt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 240

181 aaaatgagac agtctttgcc ggaattgctg catcagctag gcctgctacc ggttggaagg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 300

241 ttaagtaatt tagctcagga ggtagtctac taaatgttcc aaaacgtatc ttagggattc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 360

301 attctcttca acaatcacta aagaagttca aatgatttta aatgtatgtt tacctactag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 420

361 agaagaggta acaaaggaaa gaagaacgtt tgcgcaaaat gtttctctac tgtacgataa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 480

421 aagactaata aaaaaagata tcaaaagata aaacatgcag gaaccctcag gctttacatt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 540

481 ttagccccat accaatcatc gccgtcgcaa gtaataccat atattttcta tttttcatcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 600

541 ccgttcgctc tgtcttaccc tatgttcccg tagcagatat taatatcgat ttttaacata 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 660

601 aaattaaaca taaaaaacat taaaataaag agtaacaaaa tgaattttac ttacgcttaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 720

661 tctcttttga agttgcttta cagttttatt cgagtcgttt tatatgttaa aatccctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 780

721 gctagtcgtt gaataagagg ccacatctaa gaaaaagaag tatttaaggt tgttacattg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 840

781 acggttaact aaaaagagat ctacgattat ttaactttga ttacttatat tattgtgtcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 900

841 aaaactattt aacatttgag aatgagtttt tttcttacaa ttttgattaa attggaaaaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 960

901 tatttccgag taccgcaatt tctcctatca tttttaagat gttaaaaaat taaattacag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ScaI(1014)

961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1020

961 tgttaacaga acgcttatat taagataatt aaaagcgatt gacgacaaaa tcatgataat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1080

1021 gaaagttata cggaatatac gttgaaatta agtacttctt acgttataaa aacgttagaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1140

1081 agaacatgac tcgcgaaaaa tagtaaatca tgagtaataa attaaaaagt catttcccgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1200

1141 tatttattta aacatcttct acgttacatt agaggagata ggaaaacgag tatacaaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1260

1201 ctcatatgga tcagatcttt ccgaacataa attttataat aagttatttt taagtgttaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1320

1261 aaatgctgta cacgatggta agtgaattga aggactaata ttttaaccaa gcaaatatga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1380

1321 ttaatgaatt catggaaatt gatttgtttt acggatatat atataattaa atgttactca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(1429) ..........

1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1440

1381 cagtctattc agtgatacag gctcaccaat tcctcaatct gagcggtagc taccatacca 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1500

1441 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct gtgggcggtt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1560

1501 gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg tctgttcgac 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1620

1561 actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct 1620

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1680

1621 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1740

1681 gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1800

1741 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1800

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1860

1801 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1860

.......... .......... ....... Ampicillin ORF 2 ....... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1920

1861 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1920

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1980

1921 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1980

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2040

1981 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2040

.......... ....... Ampicillin ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2100

2041 atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg 2100

Ampicillin .......... ORF 2 .......... .......... ..........

.......... .......... ScaI(2128) .......... .......... ..........

2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2160

2101 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2220

2161 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2280

2221 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2340

2281 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2400

2341 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 2400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2460

2401 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2520

2461 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2520

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2580

2521 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2580

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2640

2581 gggcatagca tcaatagatg tgctgcccct cagtccggtg atacctactt gctttatctg 2640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2700

2641 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2760

2701 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2820

2761 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2880

2821 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2880

.......... .......... .......... pBR322_ori .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2940

2881 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2940

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3000

2941 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacaag 3000

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3060

3001 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 3060

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3120

3061 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 3120

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3180

3121 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 3180

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3240

3181 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 3240

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3300

3241 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 3300

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3360

3301 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 3360

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3420

3361 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 3420

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3480

3421 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3480

.......... pBR322_ori .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3540

3481 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3600

3541 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3660

3601 gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3720

3661 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3780

3721 tgcgttaatt acactcaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 3780

.......... .......... .......... .......... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3840

3781 gccgagcata caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac 3840

lac_promoter ........ ...... M13_pUC_rev_primer .. M13_reverse_primer

........ HindIII(3856) ........ .......... .......... ..........

3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3900

3841 tggtactaat gcggttcgaa cgtaccgccg gcgtattgaa gcatatcgta tgtaatatgc 3900

.......... .......... .......... .......... loxP ..........

.......... .......... .......... .......... .......... ..........

3901 aagttatgca tgcatcatca ccatcatcat taaggcgcgc cacttctaaa taagcgaatt 3960

3901 ttcaatacgt acgtagtagt ggtagtagta attccgcgcg gtgaagattt attcgcttaa 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat 4020

3961 agaatactaa atactaaaaa taataattta ttcaatattt tttttattca catatgttta 4020

.......... .......... .......... .......... ...... pHybLex_rev_primer

.......... .......... .......... .......... .......... ..........

4021 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt 4080

4021 aaatttcact gagaatccaa aattttgctt ttaagaataa gaactcattg agaaaggaca 4080

pHybLex_rev_primer .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg 4140

4081 tccagtccaa cgaaagagtc catatcatac tccagcgaga ataactggtg tggagatggc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... ......... BglII(4172) ......... ..........

4141 gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg 4200

4141 cgtctaggcg atccctattg tcccattata tctagacaaa tcgaacggag caggggcggc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc 4260

4201 ccagtgggcc ggtcgctgta cctccgggtc ttatgggagg aactgtcaga actgcacgcg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa 4320

4261 tcgagtcccc gtactacact gacagcgggc atgtaaatcg ggtatgtagg ggtacatatt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg 4380

4321 agtaaacgta ggtatgtaaa actaccggcg tgccgcgctt cgtttttaat gccgaggagc 4380

.......... .......... .......... .......... .......... ..........

PstI(4386) .......... .......... .......... .......... ..........

4381 ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc 4440

4381 gacgtctgga cgctcgtccc tttgcgaggg gagtgtctgc gcaacttaac aggggtgcgg 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata 4500

4441 cgcggggaca tctctttata ttttccaatc ctaaacggtg actccaagaa gaaagtatat 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca 4560

4501 gaaggaaaat tttagaacga tcctatgtca agagtgtagt gtaggcttgt atttgttggt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt 4620

4561 acccattcct tttctgagtg caaagctccg gcgctaattt aaggttgtac ctacgactaa 4620

.......... .......... kanR .......... .......... ..........

.......... .......... NruI(4643) .......... .......... ClaI(4677)

4621 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat 4680

4621 atatacccat atttacccga gcgctattac agcccgttag tccacgctgt tagatagcta 4680

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca 4740

4681 acataccctt cgggctacgc ggtctcaaca aagactttgt accgtttcca tcgcaacggt 4740

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga 4800

4741 tactacaatg tctactctac cagtctgatt tgaccgactg ccttaaatac ggagaaggct 4800

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg 4860

4801 ggtagttcgt aaaataggca tgaggactac tacgtaccaa tgagtggtga cgctaggggc 4860

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg 4920

4861 cgttttgtcg taaggtccat aatcttctta taggactaag tccactttta taacaactac 4920

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca 4980

4921 gcgaccgtca caaggacgcg gccaacgtaa gctaaggaca aacattaaca ggaaaattgt 4980

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg 5040

4981 cgctagcgca taaagcagag cgagtccgcg ttagtgctta cttattgcca aaccaactac 5040

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc 5100

5041 gctcactaaa actactgctc gcattaccga ccggacaact tgttcagacc tttctttacg 5100

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata 5160

5101 tattcgaaaa cggtaagagt ggcctaagtc agcagtgagt accactaaag agtgaactat 5160

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg 5220

5161 tggaataaaa actgctcccc tttaattatc caacataact acaacctgct cagccttagc 5220

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat 5280

5221 gtctggctat ggtcctagaa cggtaggata ccttgacgga gccactcaaa agaggaagta 5280

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt 5340

5281 atgtctttgc cgaaaaagtt tttataccat aactattagg actatactta tttaacgtca 5340

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttcatttgat gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt 5400

5341 aagtaaacta cgagctactc aaaaagatta gtcatgactg ttatttttct aagaacaaaa 5400

.......... kanR .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt 5460

5401 gttcttgaac agtaaacata tcaaaaaaat ataacatcaa caagataaaa ttagtttaca 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc 5520

5461 atcgcactaa atataaaaaa aagcggagct gtagtagacg ggtctacgct tcaattcacg 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat 5580

5521 cgtctttcat tatagtacgc agttagcata cacttacgac cagcgatatg acgacagcta 5580

.......... .......... .......... .......... .......... ..........

.......... .......... SalI(5604) .......... .......... ..........

5581 tcgatactaa cgccgccatc cagtcgacga actagtataa cttcgtatat ggtattatat 5640

5581 agctatgatt gcggcggtag gtcagctgct tgatcatatt gaagcatata ccataatata 5640

.......... .......... .......... .......... .......... ..........

.... NotI(5653),EagI(5653) .... .......... .......... ..........

5641 acgaagttat gcggccgctg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 5700

5641 tgcttcaata cgccggcgac ttaagtgacc ggcagcaaaa tgttgcagca ctgacccttt 5700

.......... .......... . M13_forward20_primer M13_pUC_fwd_primer .

.......... .......... .......... .......... ......... PvuII(5753)

5701 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 5760

5701 tgggaccgca atgggttgaa ttagcggaac gtcgtgtagg gggaaagcgg tcgaccgcat 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 5820

5761 tatcgcttct ccgggcgtgg ctagcgggaa gggttgtcaa cgcgtcggac ttaccgctta 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataccat 5880

5821 ccgcggacta cgccataaaa gaggaatgcg tagacacgcc ataaagtgtg gcgtatggta 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cgtccggatc aatgaagtag atgagtttac tacctgtata tacttttttt tttgtcaaag 5940

5881 gcaggcctag ttacttcatc tactcaaatg atggacatat atgaaaaaaa aaacagtttc 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tcacttttat ggcatttcca tgttgaagga ttagccacag cactaaattt tcagcgtatg 6000

5941 agtgaaaata ccgtaaaggt acaacttcct aatcggtgtc gtgatttaaa agtcgcatac 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 attgctttta aatatttaat tttcatcgtt ttttaattaa tattcaaaac gatttaatgc 6060

6001 taacgaaaat ttataaatta aaagtagcaa aaaattaatt ataagttttg ctaaattacg 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cttctacatc tgaagtcaag ctcatagact cgggttgggt agctaaaact acatctaaca 6120

6061 gaagatgtag acttcagttc gagtatctga gcccaaccca tcgattttga tgtagattgt 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 cgaaggggta tagccttctt aaagtatact catgtataaa cgttttgggt tcatattttt 6180

6121 gcttccccat atcggaagaa tttcatatga gtacatattt gcaaaaccca agtataaaaa 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gggccacgtg gtttataaaa attcttaact acaccactcg gtatcccgca cccgtctacg 6240

6181 cccggtgcac caaatatttt taagaattga tgtggtgagc catagggcgt gggcagatgc 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tttctacgat ttcgagctaa tattgagtaa agtgattagc aaaaaaaata aaagtactcg 6300

6241 aaagatgcta aagctcgatt ataactcatt tcactaatcg ttttttttat tttcatgagc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ttgtcggaga tcaagaattt ttctattatc tcatctaaac cactttctaa aagcgaaaaa 6360

6301 aacagcctct agttcttaaa aagataatag agtagatttg gtgaaagatt ttcgcttttt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 caaaatcgta atatgcagct tgaatgggct tccatagttt gaaagaaaaa ccctagcagt 6420

6361 gttttagcat tatacgtcga acttacccga aggtatcaaa ctttcttttt gggatcgtca 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 actggcaagg gagacattcc ttttacctgg ctaatcgcca tcttccagca ggcgcaccat 6480

6421 tgaccgttcc ctctgtaagg aaaatggacc gattagcggt agaaggtcgt ccgcgtggta 6480

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

6481 tgcccctgtt tcactatcca ggttacggat atagttcatg acaatattta cattggtcca 6540

6481 acggggacaa agtgataggt ccaatgccta tatcaagtac tgttataaat gtaaccaggt 6540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gccaccagct tgcatgatct ccggtattga aactccagcg cgggccatat ctcgcgcggc 6600

6541 cggtggtcga acgtactaga ggccataact ttgaggtcgc gcccggtata gagcgcgccg 6600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tccgacacgg gcactgtgtc cagaccaggc caggtatctc tgaccagagt catccttagc 6660

6601 aggctgtgcc cgtgacacag gtctggtccg gtccatagag actggtctca gtaggaatcg 6660

.......... .......... ORF 1 .......... .......... ..........

.......... ClaI(6674) .......... .......... .......... ..........

6661 gccgtaaatc aatcgatgag ttgcttcaaa aatcccttcc agggcgcgag ttgatagctg 6720

6661 cggcatttag ttagctactc aacgaagttt ttagggaagg tcccgcgctc aactatcgac 6720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gctggtggca gatggcgcgg caacaccatt ttttctgacc cggcaaaaca ggtagttatt 6780

6721 cgaccaccgt ctaccgcgcc gttgtggtaa aaaagactgg gccgttttgt ccatcaataa 6780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cggatcatca gctacaccag agacggaaat ccatcgctcg accagtttag ttacccccag 6840

6781 gcctagtagt cgatgtggtc tctgccttta ggtagcgagc tggtcaaatc aatgggggtc 6840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gctaagtgcc ttctctacac ctgcggtgct aaccagcgtt ttcgttctgc caatatggat 6900

6841 cgattcacgg aagagatgtg gacgccacga ttggtcgcaa aagcaagacg gttataccta 6900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 taacattctc ccaccgtcag tacgtgagat atctttaacc ctgatcctgg caatttcggc 6960

6901 attgtaagag ggtggcagtc atgcactcta tagaaattgg gactaggacc gttaaagccg 6960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tatacgtaac agggtgttat aagcaatccc cagaaatgcc agattacgta tatcctggca 7020

6961 atatgcattg tcccacaata ttcgttaggg gtctttacgg tctaatgcat ataggaccgt 7020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gcgatcgcta ttttccatga gtgaacgaac ctggtcgaaa tcagtgcgtt cgaacgctag 7080

7021 cgctagcgat aaaaggtact cacttgcttg gaccagcttt agtcacgcaa gcttgcgatc 7080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 agcctgtttt gcacgttcac cggcatcaac gttttctttt cggatccgcc gcataaccag 7140

7081 tcggacaaaa cgtgcaagtg gccgtagttg caaaagaaaa gcctaggcgg cgtattggtc 7140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tgaaacagca ttgctgtcac ttggtcgtgg cagcccggac cgacgatgaa gcatgtttag 7200

7141 actttgtcgt aacgacagtg aaccagcacc gtcgggcctg gctgctactt cgtacaaatc 7200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ctggcccaaa tgttgctgga tagtttttac tgccagaccg cgcgcctgaa gatatagaag 7260

7201 gaccgggttt acaacgacct atcaaaaatg acggtctggc gcgcggactt ctatatcttc 7260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ataatcgcga acatcttcag gttctgcggg aaaccatttc cggttattca acttgcacca 7320

7261 tattagcgct tgtagaagtc caagacgccc tttggtaaag gccaataagt tgaacgtggt 7320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tgccgcccac gaccggcaaa cggacagaag cattttccag gtatgctcag aaaacgcctg 7380

7321 acggcgggtg ctggccgttt gcctgtcttc gtaaaaggtc catacgagtc ttttgcggac 7380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 gcgatccctg aacatgtcca tcaggttctt gcgaacctca tcactcgttg catcgaccgg 7440

7381 cgctagggac ttgtacaggt agtccaagaa cgcttggagt agtgagcaac gtagctggcc 7440

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 taacttttag attaacattt gtttaaatat gaaattaggc aatgtaacat actgcaggca 7500

7441 attgaaaatc taattgtaaa caaatttata ctttaatccg ttacattgta tgacgtccgt 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NdeI(7531) .......... ..........

7501 aattttggtg tacggtaagt aaattggaca tatgatttaa caaagcgact ataagtcaga 7560

7501 ttaaaaccac atgccattca tttaacctgt atactaaatt gtttcgctga tattcagtct 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 aagtgagaat gagattgaaa taattaattc aacttattca attgatgata tgccaggatt 7620

7561 ttcactctta ctctaacttt attaattaag ttgaataagt taactactat acggtcctaa 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 cctcttcctt tatatatctt caatgtttcg attaacaggt ccattatccg gtttttagtt 7680

7621 ggagaaggaa atatatagaa gttacaaagc taattgtcca ggtaataggc caaaaatcaa 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tcgctgcaca ttgccgaatg acaatcggat tcctttaagc tttgcttttg ccctgttttc 7740

7681 agcgacgtgt aacggcttac tgttagccta aggaaattcg aaacgaaaac gggacaaaag 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 tctccccagt aatcgttggg tgactgaacc atccagtagc cgagagttac gtttttcctg 7800

7741 agaggggtca ttagcaaccc actgacttgg taggtcatcg gctctcaatg caaaaaggac 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 atttaataaa tatccatatc tatttgtctc gtggtgtttt acagcaacta accgaaatta 7860

7801 taaattattt ataggtatag ataaacagag caccacaaaa tgtcgttgat tggctttaat 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ctattttgca tcatccaacc ataaagagac aacaataatt ctagaaaaca tataacaagt 7920

7861 gataaaacgt agtaggttgg tatttctctg ttgttattaa gatcttttgt atattgttca 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 gtttttttct atttacgtct catacgcaaa gtccacttta ccatgttttg gaaacttgta 7980

7921 caaaaaaaga taaatgcaga gtatgcgttt caggtgaaat ggtacaaaac ctttgaacat 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 cccaaccatt catttttcga ttttgtaatg aggctctttt cttctaacaa tcattgaggt 8040

7981 gggttggtaa gtaaaaagct aaaacattac tccgagaaaa gaagattgtt agtaactcca 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 cattatcatg tgtcctcacc tattgtttaa attgaggctt gcgctattcc caaaattgtg 8100

8041 gtaatagtac acaggagtgg ataacaaatt taactccgaa cgcgataagg gttttaacac 8100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... KpnI(8159)

8101 ggtattattt tcatatatca gtatctgcaa tttcggttca actccggaag ctcggtaccc 8160

8101 ccataataaa agtatatagt catagacgtt aaagccaagt tgaggccttc gagccatggg 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta 8220

8161 ttgacaccct tatgagtcca tagcattcta cgttctcaag cttagagaat cgttggtaat 8220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 ttttttttct ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg 8280

8221 aaaaaaaaga gagttgtatt gctcttgtgt gtccccgcga tagcgtgtct tagtttaagc 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg 8340

8281 tactgacctt taaaaaacaa ttaaagtctc cagcggactg cgtatatgga aaaagttgac 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 aaaaattggg agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga 8400

8341 tttttaaccc tctttttcct ttccactctc gcggccttgg ccgaaaagta tatcttatct 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 gaagcgttca tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg 8460

8401 cttcgcaagt actgatttac gaacgtagtg ttatgaactt caactgttat aataaattcc 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 acctattgtt ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct 8520

8461 tggataacaa aaaaggttat ccaccaatcg ttagcagaat gaaagattga aaagaatgga 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 tttacatttc agcaatatat atatatatat atatatatat ttcaaggata taccattgta 8580

8521 aaatgtaaag tcgttatata tatatatata tatatatata aagttcctat atggtaacat 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca 8640

8581 tacagacggg gattcttcta gcagcaaaac ggtccactgg tgcaaccagt tctttagtgt 8640

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat 8700

8641 cggcttcggt aattccaaga atttcgataa agactacaag caaggttaca gttcaagcta 8700

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgttcc acttccagat 8760

8701 aagcttttag taaattaacc accacgacga tagctacgat gtccacaagg tgaaggtcta 8760

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 gaggcgctgg aagcctccaa gaaggctgat gccgttttgt taggtgctgt gggtggtcct 8820

8761 ctccgcgacc ttcggaggtt cttccgacta cggcaaaaca atccacgaca cccaccagga 8820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt 8880

8821 tttaccccat ggccatcaca atctggactt gttccaaatg atttttaggc atttcttgaa 8880

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct 8940

8881 gttaacatgc ggttgaattc tggtacattg aaacgtaggc tgagagaaaa tctgaataga 8940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgttagaga attagtggga 9000

8941 ggttagttcg gtgttaaacg atttccatga ctgaagcaac aacaatctct taatcaccct 9000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa 9060

9001 ccataaatga aaccattctc tttccttctg ctaccactac cacagcgaac cctatcactt 9060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa 9120

9061 gttatgtggc aaggtcttca cgtttcttag tgttcttacc ggcgaaagta ccgggatgtt 9120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 catgagccac cattgcctat ttggtccttg gataaagcta atgttttggc ctcttcaaga 9180

9121 gtactcggtg gtaacggata aaccaggaac ctatttcgat tacaaaaccg gagaagttct 9180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 ttatggagaa aaactgtgga ggaaaccatc aagaacgaat ttcctacatt gaaagttcaa 9240

9181 aatacctctt tttgacacct cctttggtag ttcttgctta aaggatgtaa ctttcaagtt 9240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt 9300

9241 gtagttaact aactaagacg gcggtactag gatcaattct tgggttgggt ggatttacca 9300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... ......... EcoRV(9331) ......... ..........

9301 attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca 9360

9301 taatattagt ggtcgttgta caaaccacta tagtagaggc tacttcggag gcaatagggt 9360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 ggctccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca 9420

9361 ccgaggaacc caaacaacgg tagacgcagg aaccggagaa acggtctgtt cttgtggcgt 9420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... NcoI(9442) .......... .......... ..........

9421 tttggtttgt acgaaccatg ccatggttcc gctccagatt tgccaaagaa taaggtcaac 9480

9421 aaaccaaaca tgcttggtac ggtaccaagg cgaggtctaa acggtttctt attccagttg 9480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa 9540

9481 ggatagcggt gatagaacag acgacgttac tacaacttta acagtaactt gaacggactt 9540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 gaaggtaaag ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt 9600

9541 cttccatttc ggtaacttct acgtcaattt ttccaaaacc tacgtccata gtcttgacca 9600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag 9660

9601 ctaaatccac caaggttgtc atggtggctt cagccactac gacagcggct tcttcaattc 9660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 aaaatccttg cttaaaaaga ttctcttttt ttgtgatatt tgtacataaa ctttataaat 9720

9661 ttttaggaac gaatttttct aagagaaaaa aacactataa acatgtattt gaaatattta 9720

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagacga 9780

9721 ctttaagtat tatctttgct gtgctttaat gttttacctt atacaagtat cccatctgct 9780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa 9840

9781 ttgatatatg cgttagatgt atgtaaatag ttcttcctct ttttcctcct acatttcctt 9840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta 9900

9841 atgtccattc gtttaactat gattaccgag ttgcactatt cctttttctt aacgtgaaat 9900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 acattaatat tgacaaggag gagggcatca ca........ .......... .......... 9932

9901 tgtaattata actgttcctc ctcccgtagt gt........ .......... .......... 9932

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-9932 Cross-reference: DOI; 10.1016/j.gene.2007.09.024. Cross-reference: PUBMED; 18054176. Reference author: Watson A.T., Garcia V., Bone N., Carr A.M., Armstrong J.; Reference title: "Gene tagging and gene replacement using recombinase-mediated cassette Reference title: exchange in Schizosaccharomyces pombe"; Reference location: Gene 407(1-2):63-74(2008).
Reference number: [2] Reference positions: 1-9932 Reference author: Watson A.T.; Reference title: ; Reference location: Submitted (27-JUN-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, Reference location: East Sussex BN1 9RQ, UK