Sequence of pAW8

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Sequence of pAW8_GST

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Sequence of pAW8_GST: 10595 BP; 2987 A; 2243 C; 2220 G; 3145 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 60

1 gtttttcaat ccacattgtc ttttagtact ttgatactaa agattaaata tataacctcc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 120

61 taaaagagat tttttttttt atgttgttta ttttttgtga gttactggac tggtaaacta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 180

121 cctcaaattc agttatggaa gaacttggta aagggtatta ccactttcaa gggagttctt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 240

181 aaaatgagac agtctttgcc ggaattgctg catcagctag gcctgctacc ggttggaagg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 300

241 ttaagtaatt tagctcagga ggtagtctac taaatgttcc aaaacgtatc ttagggattc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 360

301 attctcttca acaatcacta aagaagttca aatgatttta aatgtatgtt tacctactag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 420

361 agaagaggta acaaaggaaa gaagaacgtt tgcgcaaaat gtttctctac tgtacgataa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 480

421 aagactaata aaaaaagata tcaaaagata aaacatgcag gaaccctcag gctttacatt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 540

481 ttagccccat accaatcatc gccgtcgcaa gtaataccat atattttcta tttttcatcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 600

541 ccgttcgctc tgtcttaccc tatgttcccg tagcagatat taatatcgat ttttaacata 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 660

601 aaattaaaca taaaaaacat taaaataaag agtaacaaaa tgaattttac ttacgcttaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 720

661 tctcttttga agttgcttta cagttttatt cgagtcgttt tatatgttaa aatccctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 780

721 gctagtcgtt gaataagagg ccacatctaa gaaaaagaag tatttaaggt tgttacattg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 840

781 acggttaact aaaaagagat ctacgattat ttaactttga ttacttatat tattgtgtcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 900

841 aaaactattt aacatttgag aatgagtttt tttcttacaa ttttgattaa attggaaaaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 960

901 tatttccgag taccgcaatt tctcctatca tttttaagat gttaaaaaat taaattacag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ScaI(1014)

961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1020

961 tgttaacaga acgcttatat taagataatt aaaagcgatt gacgacaaaa tcatgataat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1080

1021 gaaagttata cggaatatac gttgaaatta agtacttctt acgttataaa aacgttagaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1140

1081 agaacatgac tcgcgaaaaa tagtaaatca tgagtaataa attaaaaagt catttcccgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1200

1141 tatttattta aacatcttct acgttacatt agaggagata ggaaaacgag tatacaaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1260

1201 ctcatatgga tcagatcttt ccgaacataa attttataat aagttatttt taagtgttaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1320

1261 aaatgctgta cacgatggta agtgaattga aggactaata ttttaaccaa gcaaatatga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1380

1321 ttaatgaatt catggaaatt gatttgtttt acggatatat atataattaa atgttactca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(1429) ..........

1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1440

1381 cagtctattc agtgatacag gctcaccaat tcctcaatct gagcggtagc taccatacca 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1500

1441 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct gtgggcggtt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1560

1501 gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg tctgttcgac 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1620

1561 actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct 1620

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1680

1621 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1740

1681 gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1800

1741 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1800

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1860

1801 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1860

.......... .......... ....... Ampicillin ORF 3 ....... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1920

1861 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1920

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1980

1921 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1980

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2040

1981 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2040

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2100

2041 atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg 2100

Ampicillin .......... ORF 3 .......... .......... ..........

.......... .......... ScaI(2128) .......... .......... ..........

2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2160

2101 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2220

2161 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2280

2221 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2340

2281 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2400

2341 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 2400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2460

2401 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2520

2461 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2580

2521 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2640

2581 gggcatagca tcaatagatg tgctgcccct cagtccggtg atacctactt gctttatctg 2640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2700

2641 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2760

2701 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2820

2761 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2880

2821 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2880

.......... .......... .......... pBR322_ori .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2940

2881 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2940

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3000

2941 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacaag 3000

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3060

3001 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 3060

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3120

3061 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 3120

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3180

3121 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 3180

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3240

3181 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 3240

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3300

3241 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 3300

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3360

3301 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 3360

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3420

3361 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 3420

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3480

3421 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3480

.......... pBR322_ori .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3540

3481 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3600

3541 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3660

3601 gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3720

3661 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3780

3721 tgcgttaatt acactcaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 3780

.......... .......... .......... .......... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3840

3781 gccgagcata caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac 3840

lac_promoter ........ ...... M13_pUC_rev_primer .. M13_reverse_primer

........ HindIII(3856) ........ .......... .......... ..........

3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3900

3841 tggtactaat gcggttcgaa cgtaccgccg gcgtattgaa gcatatcgta tgtaatatgc 3900

.......... .......... ORF 1 .......... loxP ..........

.......... .......... .......... .......... .......... ..........

3901 aagttatgca tgctcatgtc ccctatacta ggttattgga aaattaaggg ccttgtgcaa 3960

3901 ttcaatacgt acgagtacag gggatatgat ccaataacct tttaattccc ggaacacgtt 3960

.......... .......... ORF 1 gst .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc 4020

3961 gggtgagctg aagaaaacct tatagaactt ctttttatac ttctcgtaaa catactcgcg 4020

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt 4080

4021 ctacttccac tatttaccgc tttgtttttc aaacttaacc caaacctcaa agggttagaa 4080

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata 4140

4081 ggaataatat aactaccact acaatttaat tgtgtcagat accggtagta tgcaatatat 4140

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt 4200

4141 cgactgttcg tgttgtacaa cccaccaaca ggttttctcg cacgtctcta aagttacgaa 4200

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt 4260

4201 cttcctcgcc aaaacctata atctatgcca caaagctctt aacgtatatc atttctgaaa 4260

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat 4320

4261 ctttgagagt ttcaactaaa agaatcgttc gatggacttt acgactttta caagcttcta 4320

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... SwaI(4344) BclI(4351) .......... ..........

4321 cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg 4380

4321 gcaaatacag tattttgtat aaatttacca ctagtacatt gggtaggact gaagtacaac 4380

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa 4440

4381 atactgcgag aactacaaca aaatatgtac ctgggttaca cggacctacg caagggtttt 4440

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc 4500

4441 aatcaaacaa aattttttgc ataacttcga tagggtgttt aactattcat gaactttagg 4500

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat 4560

4501 tcgttcatat atcgtaccgg aaacgtcccg accgttcggt gcaaaccacc accgctggta 4560

.......... .......... ORF 1 gst .......... .......... ..........

.......... .......... .......... AscI(4593) .......... ..........

4561 cctccaaaat cggatctggt tccgcgttga ggcgcgccag atctacttct aaataagcga 4620

4561 ggaggtttta gcctagacca aggcgcaact ccgcgcggtc tagatgaaga tttattcgct 4620

.......... ORF 1 gst .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca 4680

4621 taaagaatac taaatactaa aaataataat ttattcaata ttttttttat tcacatatgt 4680

.......... .......... .......... .......... ...... pHybLex_rev_primer

.......... .......... .......... .......... .......... ..........

4681 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc 4740

4681 ttaaaatttc actgagaatc caaaattttg cttttaagaa taagaactca ttgagaaagg 4740

pHybLex_rev_primer .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta 4800

4741 acatccagtc caacgaaaga gtccatatca tactccagcg agaataactg gtgtggagat 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccg 4860

4801 ggccgtctag gcgatcccta ttgtcccatt atatctagac aaatcgaacg gagcaggggc 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg 4920

4861 ggcccagtgg gccggtcgct gtacctccgg gtcttatggg aggaactgtc agaactgcac 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta 4980

4921 gcgtcgagtc cccgtactac actgacagcg ggcatgtaaa tcgggtatgt aggggtacat 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc 5040

4981 attagtaaac gtaggtatgt aaaactaccg gcgtgccgcg cttcgttttt aatgccgagg 5040

.......... .......... .......... .......... .......... ..........

PstI(5049) .......... .......... .......... MluI(5082) ..........

5041 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac 5100

5041 agcgacgtct ggacgctcgt ccctttgcga ggggagtgtc tgcgcaactt aacaggggtg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat 5160

5101 cggcgcgggg acatctcttt atattttcca atcctaaacg gtgactccaa gaagaaagta 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa 5220

5161 tatgaaggaa aattttagaa cgatcctatg tcaagagtgt agtgtaggct tgtatttgtt 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac atggatgctg 5280

5221 ggtacccatt ccttttctga gtgcaaagct ccggcgctaa tttaaggttg tacctacgac 5280

.......... .......... .......... kanR .......... ..........

.......... .......... NruI(5306) .......... .......... ..........

5281 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 5340

5281 taaatatacc catatttacc cgagcgctat tacagcccgt tagtccacgc tgttagatag 5340

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 5400

5341 ctaacatacc cttcgggcta cgcggtctca acaaagactt tgtaccgttt ccatcgcaac 5400

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 5460

5401 ggttactaca atgtctactc taccagtctg atttgaccga ctgccttaaa tacggagaag 5460

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 5520

5461 gctggtagtt cgtaaaatag gcatgaggac tactacgtac caatgagtgg tgacgctagg 5520

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 5580

5521 ggccgttttg tcgtaaggtc cataatcttc ttataggact aagtccactt ttataacaac 5580

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 5640

5581 tacgcgaccg tcacaaggac gcggccaacg taagctaagg acaaacatta acaggaaaat 5640

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 5700

5641 tgtcgctagc gcataaagca gagcgagtcc gcgttagtgc ttacttattg ccaaaccaac 5700

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 5760

5701 tacgctcact aaaactactg ctcgcattac cgaccggaca acttgttcag acctttcttt 5760

.......... .......... kanR .......... .......... ..........

HindIII(5767) ...... .......... .......... .......... ..........

5761 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 5820

5761 acgtattcga aaacggtaag agtggcctaa gtcagcagtg agtaccacta aagagtgaac 5820

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 5880

5821 tattggaata aaaactgctc ccctttaatt atccaacata actacaacct gctcagcctt 5880

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 5940

5881 agcgtctggc tatggtccta gaacggtagg ataccttgac ggagccactc aaaagaggaa 5940

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 6000

5941 gtaatgtctt tgccgaaaaa gtttttatac cataactatt aggactatac ttatttaacg 6000

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt 6060

6001 tcaaagtaaa ctacgagcta ctcaaaaaga ttagtcatga ctgttatttt tctaagaaca 6060

.......... kanR .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa 6120

6061 aaagttcttg aacagtaaac atatcaaaaa aatataacat caacaagata aaattagttt 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag 6180

6121 acaatcgcac taaatataaa aaaaagcgga gctgtagtag acgggtctac gcttcaattc 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc 6240

6181 acgcgtcttt cattatagta cgcagttagc atacacttac gaccagcgat atgacgacag 6240

.......... .......... .......... .......... .......... ..........

.......... .......... SalI(6267) .......... .......... ..........

6241 gattcgatac taacgccgcc atccagtcga cgaactagta taacttcgta tatggtatta 6300

6241 ctaagctatg attgcggcgg taggtcagct gcttgatcat attgaagcat ataccataat 6300

.......... .......... .......... .......... .......... ..........

.... NotI(6316),EagI(6316) .... .......... .......... ..........

6301 tatacgaagt tatgcggccg ctgaattcac tggccgtcgt tttacaacgt cgtgactggg 6360

6301 atatgcttca atacgccggc gacttaagtg accggcagca aaatgttgca gcactgaccc 6360

.......... .......... . M13_forward20_primer M13_pUC_fwd_primer .

.......... .......... .......... .......... .......... ..........

6361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 6420

6361 ttttgggacc gcaatgggtt gaattagcgg aacgtcgtgt agggggaaag cggtcgaccg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 6480

6421 cattatcgct tctccgggcg tggctagcgg gaagggttgt caacgcgtcg gacttaccgc 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac 6540

6481 ttaccgcgga ctacgccata aaagaggaat gcgtagacac gccataaagt gtggcgtatg 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 catcgtccgg atcaatgaag tagatgagtt tactacctgt atatactttt ttttttgtca 6600

6541 gtagcaggcc tagttacttc atctactcaa atgatggaca tatatgaaaa aaaaaacagt 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 aagtcacttt tatggcattt ccatgttgaa ggattagcca cagcactaaa ttttcagcgt 6660

6601 ttcagtgaaa ataccgtaaa ggtacaactt cctaatcggt gtcgtgattt aaaagtcgca 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... PacI(6701) ..........

6661 atgattgctt ttaaatattt aattttcatc gttttttaat taatattcaa aacgatttaa 6720

6661 tactaacgaa aatttataaa ttaaaagtag caaaaaatta attataagtt ttgctaaatt 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tgccttctac atctgaagtc aagctcatag actcgggttg ggtagctaaa actacatcta 6780

6721 acggaagatg tagacttcag ttcgagtatc tgagcccaac ccatcgattt tgatgtagat 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 acacgaaggg gtatagcctt cttaaagtat actcatgtat aaacgttttg ggttcatatt 6840

6781 tgtgcttccc catatcggaa gaatttcata tgagtacata tttgcaaaac ccaagtataa 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 tttgggccac gtggtttata aaaattctta actacaccac tcggtatccc gcacccgtct 6900

6841 aaacccggtg caccaaatat ttttaagaat tgatgtggtg agccataggg cgtgggcaga 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 acgtttctac gatttcgagc taatattgag taaagtgatt agcaaaaaaa ataaaagtac 6960

6901 tgcaaagatg ctaaagctcg attataactc atttcactaa tcgttttttt tattttcatg 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tcgttgtcgg agatcaagaa tttttctatt atctcatcta aaccactttc taaaagcgaa 7020

6961 agcaacagcc tctagttctt aaaaagataa tagagtagat ttggtgaaag attttcgctt 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 aaacaaaatc gtaatatgca gcttgaatgg gcttccatag tttgaaagaa aaaccctagc 7080

7021 tttgttttag cattatacgt cgaacttacc cgaaggtatc aaactttctt tttgggatcg 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 agtactggca agggagacat tccttttacc tggctaatcg ccatcttcca gcaggcgcac 7140

7081 tcatgaccgt tccctctgta aggaaaatgg accgattagc ggtagaaggt cgtccgcgtg 7140

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

7141 cattgcccct gtttcactat ccaggttacg gatatagttc atgacaatat ttacattggt 7200

7141 gtaacgggga caaagtgata ggtccaatgc ctatatcaag tactgttata aatgtaacca 7200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ccagccacca gcttgcatga tctccggtat tgaaactcca gcgcgggcca tatctcgcgc 7260

7201 ggtcggtggt cgaacgtact agaggccata actttgaggt cgcgcccggt atagagcgcg 7260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ggctccgaca cgggcactgt gtccagacca ggccaggtat ctctgaccag agtcatcctt 7320

7261 ccgaggctgt gcccgtgaca caggtctggt ccggtccata gagactggtc tcagtaggaa 7320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 agcgccgtaa atcaatcgat gagttgcttc aaaaatccct tccagggcgc gagttgatag 7380

7321 tcgcggcatt tagttagcta ctcaacgaag tttttaggga aggtcccgcg ctcaactatc 7380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 ctggctggtg gcagatggcg cggcaacacc attttttctg acccggcaaa acaggtagtt 7440

7381 gaccgaccac cgtctaccgc gccgttgtgg taaaaaagac tgggccgttt tgtccatcaa 7440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 attcggatca tcagctacac cagagacgga aatccatcgc tcgaccagtt tagttacccc 7500

7441 taagcctagt agtcgatgtg gtctctgcct ttaggtagcg agctggtcaa atcaatgggg 7500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 caggctaagt gccttctcta cacctgcggt gctaaccagc gttttcgttc tgccaatatg 7560

7501 gtccgattca cggaagagat gtggacgcca cgattggtcg caaaagcaag acggttatac 7560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... ......... EcoRV(7594) ......... ..........

7561 gattaacatt ctcccaccgt cagtacgtga gatatcttta accctgatcc tggcaatttc 7620

7561 ctaattgtaa gagggtggca gtcatgcact ctatagaaat tgggactagg accgttaaag 7620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 ggctatacgt aacagggtgt tataagcaat ccccagaaat gccagattac gtatatcctg 7680

7621 ccgatatgca ttgtcccaca atattcgtta ggggtcttta cggtctaatg catataggac 7680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 gcagcgatcg ctattttcca tgagtgaacg aacctggtcg aaatcagtgc gttcgaacgc 7740

7681 cgtcgctagc gataaaaggt actcacttgc ttggaccagc tttagtcacg caagcttgcg 7740

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 tagagcctgt tttgcacgtt caccggcatc aacgttttct tttcggatcc gccgcataac 7800

7741 atctcggaca aaacgtgcaa gtggccgtag ttgcaaaaga aaagcctagg cggcgtattg 7800

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 cagtgaaaca gcattgctgt cacttggtcg tggcagcccg gaccgacgat gaagcatgtt 7860

7801 gtcactttgt cgtaacgaca gtgaaccagc accgtcgggc ctggctgcta cttcgtacaa 7860

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 tagctggccc aaatgttgct ggatagtttt tactgccaga ccgcgcgcct gaagatatag 7920

7861 atcgaccggg tttacaacga cctatcaaaa atgacggtct ggcgcgcgga cttctatatc 7920

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 aagataatcg cgaacatctt caggttctgc gggaaaccat ttccggttat tcaacttgca 7980

7921 ttctattagc gcttgtagaa gtccaagacg ccctttggta aaggccaata agttgaacgt 7980

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ccatgccgcc cacgaccggc aaacggacag aagcattttc caggtatgct cagaaaacgc 8040

7981 ggtacggcgg gtgctggccg tttgcctgtc ttcgtaaaag gtccatacga gtcttttgcg 8040

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ctggcgatcc ctgaacatgt ccatcaggtt cttgcgaacc tcatcactcg ttgcatcgac 8100

8041 gaccgctagg gacttgtaca ggtagtccaa gaacgcttgg agtagtgagc aacgtagctg 8100

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 cggtaacttt tagattaaca tttgtttaaa tatgaaatta ggcaatgtaa catactgcag 8160

8101 gccattgaaa atctaattgt aaacaaattt atactttaat ccgttacatt gtatgacgtc 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 gcaaattttg gtgtacggta agtaaattgg acatatgatt taacaaagcg actataagtc 8220

8161 cgtttaaaac cacatgccat tcatttaacc tgtatactaa attgtttcgc tgatattcag 8220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 agaaagtgag aatgagattg aaataattaa ttcaacttat tcaattgatg atatgccagg 8280

8221 tctttcactc ttactctaac tttattaatt aagttgaata agttaactac tatacggtcc 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 attcctcttc ctttatatat cttcaatgtt tcgattaaca ggtccattat ccggttttta 8340

8281 taaggagaag gaaatatata gaagttacaa agctaattgt ccaggtaata ggccaaaaat 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ........ HindIII(8381) ........

8341 gtttcgctgc acattgccga atgacaatcg gattccttta agctttgctt ttgccctgtt 8400

8341 caaagcgacg tgtaacggct tactgttagc ctaaggaaat tcgaaacgaa aacgggacaa 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 ttctctcccc agtaatcgtt gggtgactga accatccagt agccgagagt tacgtttttc 8460

8401 aagagagggg tcattagcaa cccactgact tggtaggtca tcggctctca atgcaaaaag 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ctgatttaat aaatatccat atctatttgt ctcgtggtgt tttacagcaa ctaaccgaaa 8520

8461 gactaaatta tttataggta tagataaaca gagcaccaca aaatgtcgtt gattggcttt 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 ttactatttt gcatcatcca accataaaga gacaacaata attctagaaa acatataaca 8580

8521 aatgataaaa cgtagtaggt tggtatttct ctgttgttat taagatcttt tgtatattgt 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 agtgtttttt tctatttacg tctcatacgc aaagtccact ttaccatgtt ttggaaactt 8640

8581 tcacaaaaaa agataaatgc agagtatgcg tttcaggtga aatggtacaa aacctttgaa 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 gtacccaacc attcattttt cgattttgta atgaggctct tttcttctaa caatcattga 8700

8641 catgggttgg taagtaaaaa gctaaaacat tactccgaga aaagaagatt gttagtaact 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 ggtcattatc atgtgtcctc acctattgtt taaattgagg cttgcgctat tcccaaaatt 8760

8701 ccagtaatag tacacaggag tggataacaa atttaactcc gaacgcgata agggttttaa 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 gtgggtatta ttttcatata tcagtatctg caatttcggt tcaactccgg aagctcggta 8820

8761 cacccataat aaaagtatat agtcatagac gttaaagcca agttgaggcc ttcgagccat 8820

.......... .......... .......... .......... .......... ..........

KpnI(8822) .......... .......... .......... .......... ..........

8821 cccaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca 8880

8821 gggttgacac ccttatgagt ccatagcatt ctacgttctc aagcttagag aatcgttggt 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 ttattttttt tctctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat 8940

8881 aataaaaaaa agagagttgt attgctcttg tgtgtccccg cgatagcgtg tcttagttta 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa 9000

8941 agctactgac ctttaaaaaa caattaaagt ctccagcgga ctgcgtatat ggaaaaagtt 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat 9060

9001 gactttttaa ccctcttttt cctttccact ctcgcggcct tggccgaaaa gtatatctta 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta 9120

9061 tctcttcgca agtactgatt tacgaacgta gtgttatgaa cttcaactgt tataataaat 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta 9180

9121 tcctggataa caaaaaaggt tatccaccaa tcgttagcag aatgaaagat tgaaaagaat 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 ccttttacat ttcagcaata tatatatata tatatatata tatttcaagg atataccatt 9240

9181 ggaaaatgta aagtcgttat atatatatat atatatatat ataaagttcc tatatggtaa 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 gtaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc 9300

9241 cattacagac ggggattctt ctagcagcaa aacggtccac tggtgcaacc agttctttag 9300

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

9301 acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc 9360

9301 tgtcggcttc ggtaattcca agaatttcga taaagactac aagcaaggtt acagttcaag 9360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt tccacttcca 9420

9361 ctaaagcttt tagtaaatta accaccacga cgatagctac gatgtccaca aggtgaaggt 9420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 gatgaggcgc tggaagcctc caagaaggct gatgccgttt tgttaggtgc tgtgggtggt 9480

9421 ctactccgcg accttcggag gttcttccga ctacggcaaa acaatccacg acacccacca 9480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 cctaaatggg gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa 9540

9481 ggatttaccc catggccatc acaatctgga cttgttccaa atgattttta ggcatttctt 9540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta 9600

9541 gaagttaaca tgcggttgaa ttctggtaca ttgaaacgta ggctgagaga aaatctgaat 9600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgttag agaattagtg 9660

9601 agaggttagt tcggtgttaa acgatttcca tgactgaagc aacaacaatc tcttaatcac 9660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt 9720

9661 cctccataaa tgaaaccatt ctctttcctt ctgctaccac taccacagcg aaccctatca 9720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta 9780

9721 cttgttatgt ggcaaggtct tcacgtttct tagtgttctt accggcgaaa gtaccgggat 9780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt ggcctcttca 9840

9781 gttgtactcg gtggtaacgg ataaaccagg aacctatttc gattacaaaa ccggagaagt 9840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 agattatgga gaaaaactgt ggaggaaacc atcaagaacg aatttcctac attgaaagtt 9900

9841 tctaatacct ctttttgaca cctcctttgg tagttcttgc ttaaaggatg taactttcaa 9900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat 9960

9901 gttgtagtta actaactaag acggcggtac taggatcaat tcttgggttg ggtggattta 9960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... ......... EcoRV(9994) ......... ..........

9961 ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc 10020

9961 ccataatatt agtggtcgtt gtacaaacca ctatagtaga ggctacttcg gaggcaatag 10020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 ccaggctcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc 10080

10021 ggtccgagga acccaaacaa cggtagacgc aggaaccgga gaaacggtct gttcttgtgg 10080

.......... .......... ORF 2 .......... .......... ..........

.......... ......... NcoI(10105) ......... .......... ..........

10081 gcatttggtt tgtacgaacc atgccatggt tccgctccag atttgccaaa gaataaggtc 10140

10081 cgtaaaccaa acatgcttgg tacggtacca aggcgaggtc taaacggttt cttattccag 10140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 aaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct 10200

10141 ttgggatagc ggtgatagaa cagacgacgt tactacaact ttaacagtaa cttgaacgga 10200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 gaagaaggta aagccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact 10260

10201 cttcttccat ttcggtaact tctacgtcaa tttttccaaa acctacgtcc atagtcttga 10260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt 10320

10261 ccactaaatc caccaaggtt gtcatggtgg cttcagccac tacgacagcg gcttcttcaa 10320

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 aagaaaatcc ttgcttaaaa agattctctt tttttgtgat atttgtacat aaactttata 10380

10321 ttcttttagg aacgaatttt tctaagagaa aaaaacacta taaacatgta tttgaaatat 10380

ORF 2 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga 10440

10381 ttactttaag tattatcttt gctgtgcttt aatgttttac cttatacaag tatcccatct 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga ggatgtaaag 10500

10441 gctttgatat atgcgttaga tgtatgtaaa tagttcttcc tctttttcct cctacatttc 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa gaattgcact 10560

10501 cttatgtcca ttcgtttaac tatgattacc gagttgcact attccttttt cttaacgtga 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 ttaacattaa tattgacaag gaggagggca tcaca..... .......... .......... 10595

10561 aattgtaatt ataactgttc ctcctcccgt agtgt..... .......... .......... 10595

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-10595 Cross-reference: DOI; 10.1016/j.gene.2007.09.024. Cross-reference: PUBMED; 18054176. Reference author: Watson A.T., Garcia V., Bone N., Carr A.M., Armstrong J.; Reference title: "Gene tagging and gene replacement using recombinase-mediated cassette Reference title: exchange in Schizosaccharomyces pombe"; Reference location: Gene 407(1-2):63-74(2008).
Reference number: [2] Reference positions: 1-10595 Reference author: Watson A.T.; Reference title: ; Reference location: Submitted (27-JUN-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, Reference location: East Sussex BN1 9RQ, UK