Sequence of pAW8

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Sequence of pAW8_TAP

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Sequence of pAW8_TAP: 10466 BP; 3004 A; 2247 C; 2159 G; 3056 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 60

1 gtttttcaat ccacattgtc ttttagtact ttgatactaa agattaaata tataacctcc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 120

61 taaaagagat tttttttttt atgttgttta ttttttgtga gttactggac tggtaaacta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 180

121 cctcaaattc agttatggaa gaacttggta aagggtatta ccactttcaa gggagttctt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 240

181 aaaatgagac agtctttgcc ggaattgctg catcagctag gcctgctacc ggttggaagg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 300

241 ttaagtaatt tagctcagga ggtagtctac taaatgttcc aaaacgtatc ttagggattc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 360

301 attctcttca acaatcacta aagaagttca aatgatttta aatgtatgtt tacctactag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 420

361 agaagaggta acaaaggaaa gaagaacgtt tgcgcaaaat gtttctctac tgtacgataa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 480

421 aagactaata aaaaaagata tcaaaagata aaacatgcag gaaccctcag gctttacatt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 540

481 ttagccccat accaatcatc gccgtcgcaa gtaataccat atattttcta tttttcatcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 600

541 ccgttcgctc tgtcttaccc tatgttcccg tagcagatat taatatcgat ttttaacata 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 660

601 aaattaaaca taaaaaacat taaaataaag agtaacaaaa tgaattttac ttacgcttaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 720

661 tctcttttga agttgcttta cagttttatt cgagtcgttt tatatgttaa aatccctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 780

721 gctagtcgtt gaataagagg ccacatctaa gaaaaagaag tatttaaggt tgttacattg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 840

781 acggttaact aaaaagagat ctacgattat ttaactttga ttacttatat tattgtgtcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 900

841 aaaactattt aacatttgag aatgagtttt tttcttacaa ttttgattaa attggaaaaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 960

901 tatttccgag taccgcaatt tctcctatca tttttaagat gttaaaaaat taaattacag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ScaI(1014)

961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1020

961 tgttaacaga acgcttatat taagataatt aaaagcgatt gacgacaaaa tcatgataat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1080

1021 gaaagttata cggaatatac gttgaaatta agtacttctt acgttataaa aacgttagaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1140

1081 agaacatgac tcgcgaaaaa tagtaaatca tgagtaataa attaaaaagt catttcccgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1200

1141 tatttattta aacatcttct acgttacatt agaggagata ggaaaacgag tatacaaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1260

1201 ctcatatgga tcagatcttt ccgaacataa attttataat aagttatttt taagtgttaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1320

1261 aaatgctgta cacgatggta agtgaattga aggactaata ttttaaccaa gcaaatatga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1380

1321 ttaatgaatt catggaaatt gatttgtttt acggatatat atataattaa atgttactca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(1429) ..........

1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1440

1381 cagtctattc agtgatacag gctcaccaat tcctcaatct gagcggtagc taccatacca 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1500

1441 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct gtgggcggtt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1560

1501 gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg tctgttcgac 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1620

1561 actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct 1620

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1680

1621 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1740

1681 gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1800

1741 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1800

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1860

1801 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1860

.......... .......... ....... Ampicillin ORF 3 ....... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1920

1861 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1920

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1980

1921 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1980

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2040

1981 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2040

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2100

2041 atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg 2100

Ampicillin .......... ORF 3 .......... .......... ..........

.......... .......... ScaI(2128) .......... .......... ..........

2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2160

2101 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2220

2161 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2280

2221 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2340

2281 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2400

2341 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 2400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2460

2401 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2520

2461 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2580

2521 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2640

2581 gggcatagca tcaatagatg tgctgcccct cagtccggtg atacctactt gctttatctg 2640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2700

2641 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2760

2701 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2820

2761 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2880

2821 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2880

.......... .......... .......... pBR322_ori .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2940

2881 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2940

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3000

2941 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacaag 3000

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3060

3001 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 3060

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3120

3061 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 3120

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3180

3121 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 3180

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3240

3181 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 3240

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3300

3241 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 3300

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3360

3301 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 3360

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3420

3361 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 3420

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3480

3421 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3480

.......... pBR322_ori .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3540

3481 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3600

3541 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3660

3601 gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3720

3661 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3780

3721 tgcgttaatt acactcaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 3780

.......... .......... .......... .......... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3840

3781 gccgagcata caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac 3840

lac_promoter ........ ...... M13_pUC_rev_primer .. M13_reverse_primer

........ HindIII(3856) ........ .......... .......... ..........

3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3900

3841 tggtactaat gcggttcgaa cgtaccgccg gcgtattgaa gcatatcgta tgtaatatgc 3900

.......... .......... ORF 1 .......... loxP ..........

.......... .......... .......... .......... .......... ..........

3901 aagttatgca tgcttatgaa gcgacgatgg aaaaagaatt tcatagccgt ctcagcagcc 3960

3901 ttcaatacgt acgaatactt cgctgctacc tttttcttaa agtatcggca gagtcgtcgg 3960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aaccgcttta agaaaatctc atcctccggg gcacttgatt atggtacccc aactactgct 4020

3961 ttggcgaaat tcttttagag taggaggccc cgtgaactaa taccatgggg ttgatgacga 4020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tctgaaaatc tatattttca aggtgaacta aaaactgctg ctttggctca acatgcgatt 4080

4021 agacttttag atataaaagt tccacttgat ttttgacgac gaaaccgagt tgtacgctaa 4080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aaagctgatg cgcaacaaaa taacttcaac aaagatcaac aaagcgcctt ctatgaaatc 4140

4081 tttcgactac gcgttgtttt attgaagttg tttctagttg tttcgcggaa gatactttag 4140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttgaacatgc ctaacttaaa cgaagcgcaa cgtaacggct tcattcaaag tcttaaagac 4200

4141 aacttgtacg gattgaattt gcttcgcgtt gcattgccga agtaagtttc agaatttctg 4200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gacccaagcc aaagcactaa cgttttaggt gaagctaaaa aattaaacga atctcaagca 4260

4201 ctgggttcgg tttcgtgatt gcaaaatcca cttcgatttt ttaatttgct tagagttcgt 4260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccgaaagctg ataacaattt caacaaagaa caacaaaatg ctttctatga aatcttgaat 4320

4261 ggctttcgac tattgttaaa gttgtttctt gttgttttac gaaagatact ttagaactta 4320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... ........ HindIII(4363) ........

4321 atgcctaact taaacgaaga acaacgcaat ggtttcatcc aaagcttaaa agatgaccca 4380

4321 tacggattga atttgcttct tgttgcgtta ccaaagtagg tttcgaattt tctactgggt 4380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agccaaagtg ctaacctatt gtcagaagct aaaaagttaa atgaatctca agcaccgaaa 4440

4381 tcggtttcac gattggataa cagtcttcga tttttcaatt tacttagagt tcgtggcttt 4440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcggataaca aattcaacaa agaataaggc gcgccacttc taaataagcg aatttcttat 4500

4441 cgcctattgt ttaagttgtt tcttattccg cgcggtgaag atttattcgc ttaaagaata 4500

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa 4560

4501 ctaaatacta aaaataataa tttattcaat atttttttta ttcacatatg tttaaaattt 4560

.......... .......... .......... .......... ...... pHybLex_rev_primer

.......... .......... .......... .......... .......... ..........

4561 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca 4620

4561 cactgagaat ccaaaatttt gcttttaaga ataagaactc attgagaaag gacatccagt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat 4680

4621 ccaacgaaag agtccatatc atactccagc gagaataact ggtgtggaga tggccgtcta 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccgctaggga taacagggta atatagatct gtttagcttg cctcgtcccc gccgggtcac 4740

4681 ggcgatccct attgtcccat tatatctaga caaatcgaac ggagcagggg cggcccagtg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca 4800

4741 ggccggtcgc tgtacctccg ggtcttatgg gaggaactgt cagaactgca cgcgtcgagt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt 4860

4801 ccccgtacta cactgacagc gggcatgtaa atcgggtatg taggggtaca tattagtaaa 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag 4920

4861 cgtaggtatg taaaactacc ggcgtgccgc gcttcgtttt taatgccgag gagcgacgtc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc 4980

4921 tggacgctcg tccctttgcg aggggagtgt ctgcgcaact taacaggggt gcggcgcggg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct 5040

4981 gacatctctt tatattttcc aatcctaaac ggtgactcca agaagaaagt atatgaagga 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NcoI(5093)

5041 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgggta 5100

5041 aaattttaga acgatcctat gtcaagagtg tagtgtaggc ttgtatttgt tggtacccat 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aggaaaagac tcacgtttcg aggccgcgat taaattccaa catggatgct gatttatatg 5160

5101 tccttttctg agtgcaaagc tccggcgcta atttaaggtt gtacctacga ctaaatatac 5160

.......... .......... kanR .......... .......... ..........

.......... NruI(5177) .......... .......... .......... ..........

5161 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg 5220

5161 ccatatttac ccgagcgcta ttacagcccg ttagtccacg ctgttagata gctaacatac 5220

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg 5280

5221 ccttcgggct acgcggtctc aacaaagact ttgtaccgtt tccatcgcaa cggttactac 5280

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca 5340

5281 aatgtctact ctaccagtct gatttgaccg actgccttaa atacggagaa ggctggtagt 5340

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggcaaaa 5400

5341 tcgtaaaata ggcatgagga ctactacgta ccaatgagtg gtgacgctag gggccgtttt 5400

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg 5460

5401 gtcgtaaggt ccataatctt cttataggac taagtccact tttataacaa ctacgcgacc 5460

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc 5520

5461 gtcacaagga cgcggccaac gtaagctaag gacaaacatt aacaggaaaa ttgtcgctag 5520

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg 5580

5521 cgcataaagc agagcgagtc cgcgttagtg cttacttatt gccaaaccaa ctacgctcac 5580

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... ........ HindIII(5638)

5581 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc 5640

5581 taaaactact gctcgcatta ccgaccggac aacttgttca gacctttctt tacgtattcg 5640

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta 5700

5641 aaaacggtaa gagtggccta agtcagcagt gagtaccact aaagagtgaa ctattggaat 5700

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc 5760

5701 aaaaactgct cccctttaat tatccaacat aactacaacc tgctcagcct tagcgtctgg 5760

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga 5820

5761 ctatggtcct agaacggtag gataccttga cggagccact caaaagagga agtaatgtct 5820

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt 5880

5821 ttgccgaaaa agtttttata ccataactat taggactata cttatttaac gtcaaagtaa 5880

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tgatgctcga tgagtttttc taatcagtac tgacaataaa aagattcttg ttttcaagaa 5940

5881 actacgagct actcaaaaag attagtcatg actgttattt ttctaagaac aaaagttctt 5940

.......... kanR .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt 6000

5941 gaacagtaaa catatcaaaa aaatataaca tcaacaagat aaaattagtt tacaatcgca 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa 6060

6001 ctaaatataa aaaaaagcgg agctgtagta gacgggtcta cgcttcaatt cacgcgtctt 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata 6120

6061 tcattatagt acgcagttag catacactta cgaccagcga tatgacgaca gctaagctat 6120

.......... .......... .......... .......... .......... ..........

.......... SalI(6138) .......... .......... .......... ..........

6121 ctaacgccgc catccagtcg acgaactagt ataacttcgt atatggtatt atatacgaag 6180

6121 gattgcggcg gtaggtcagc tgcttgatca tattgaagca tataccataa tatatgcttc 6180

.......... .......... .......... .......... .......... ..........

NotI(6187),EagI(6187) ........ .......... .......... ..........

6181 ttatgcggcc gctgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 6240

6181 aatacgccgg cgacttaagt gaccggcagc aaaatgttgc agcactgacc cttttgggac 6240

.......... . M13_forward20_primer M13_pUC_fwd_primer . ..........

.......... .......... .......... .......... .......... ..........

6241 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 6300

6241 cgcaatgggt tgaattagcg gaacgtcgtg tagggggaaa gcggtcgacc gcattatcgc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc 6360

6301 ttctccgggc gtggctagcg ggaagggttg tcaacgcgtc ggacttaccg cttaccgcgg 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata ccatcgtccg 6420

6361 actacgccat aaaagaggaa tgcgtagaca cgccataaag tgtggcgtat ggtagcaggc 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gatcaatgaa gtagatgagt ttactacctg tatatacttt tttttttgtc aaagtcactt 6480

6421 ctagttactt catctactca aatgatggac atatatgaaa aaaaaaacag tttcagtgaa 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ttatggcatt tccatgttga aggattagcc acagcactaa attttcagcg tatgattgct 6540

6481 aataccgtaa aggtacaact tcctaatcgg tgtcgtgatt taaaagtcgc atactaacga 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... PacI(6572) .......... ..........

6541 tttaaatatt taattttcat cgttttttaa ttaatattca aaacgattta atgccttcta 6600

6541 aaatttataa attaaaagta gcaaaaaatt aattataagt tttgctaaat tacggaagat 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 catctgaagt caagctcata gactcgggtt gggtagctaa aactacatct aacacgaagg 6660

6601 gtagacttca gttcgagtat ctgagcccaa cccatcgatt ttgatgtaga ttgtgcttcc 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ggtatagcct tcttaaagta tactcatgta taaacgtttt gggttcatat ttttgggcca 6720

6661 ccatatcgga agaatttcat atgagtacat atttgcaaaa cccaagtata aaaacccggt 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cgtggtttat aaaaattctt aactacacca ctcggtatcc cgcacccgtc tacgtttcta 6780

6721 gcaccaaata tttttaagaa ttgatgtggt gagccatagg gcgtgggcag atgcaaagat 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cgatttcgag ctaatattga gtaaagtgat tagcaaaaaa aataaaagta ctcgttgtcg 6840

6781 gctaaagctc gattataact catttcacta atcgtttttt ttattttcat gagcaacagc 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gagatcaaga atttttctat tatctcatct aaaccacttt ctaaaagcga aaaacaaaat 6900

6841 ctctagttct taaaaagata atagagtaga tttggtgaaa gattttcgct ttttgtttta 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cgtaatatgc agcttgaatg ggcttccata gtttgaaaga aaaaccctag cagtactggc 6960

6901 gcattatacg tcgaacttac ccgaaggtat caaactttct ttttgggatc gtcatgaccg 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 aagggagaca ttccttttac ctggctaatc gccatcttcc agcaggcgca ccattgcccc 7020

6961 ttccctctgt aaggaaaatg gaccgattag cggtagaagg tcgtccgcgt ggtaacgggg 7020

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

7021 tgtttcacta tccaggttac ggatatagtt catgacaata tttacattgg tccagccacc 7080

7021 acaaagtgat aggtccaatg cctatatcaa gtactgttat aaatgtaacc aggtcggtgg 7080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 agcttgcatg atctccggta ttgaaactcc agcgcgggcc atatctcgcg cggctccgac 7140

7081 tcgaacgtac tagaggccat aactttgagg tcgcgcccgg tatagagcgc gccgaggctg 7140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 acgggcactg tgtccagacc aggccaggta tctctgacca gagtcatcct tagcgccgta 7200

7141 tgcccgtgac acaggtctgg tccggtccat agagactggt ctcagtagga atcgcggcat 7200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 aatcaatcga tgagttgctt caaaaatccc ttccagggcg cgagttgata gctggctggt 7260

7201 ttagttagct actcaacgaa gtttttaggg aaggtcccgc gctcaactat cgaccgacca 7260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ggcagatggc gcggcaacac cattttttct gacccggcaa aacaggtagt tattcggatc 7320

7261 ccgtctaccg cgccgttgtg gtaaaaaaga ctgggccgtt ttgtccatca ataagcctag 7320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 atcagctaca ccagagacgg aaatccatcg ctcgaccagt ttagttaccc ccaggctaag 7380

7321 tagtcgatgt ggtctctgcc tttaggtagc gagctggtca aatcaatggg ggtccgattc 7380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 tgccttctct acacctgcgg tgctaaccag cgttttcgtt ctgccaatat ggattaacat 7440

7381 acggaagaga tgtggacgcc acgattggtc gcaaaagcaa gacggttata cctaattgta 7440

.......... .......... ORF 1 .......... .......... ..........

.......... ......... EcoRV(7465) ......... .......... ..........

7441 tctcccaccg tcagtacgtg agatatcttt aaccctgatc ctggcaattt cggctatacg 7500

7441 agagggtggc agtcatgcac tctatagaaa ttgggactag gaccgttaaa gccgatatgc 7500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 taacagggtg ttataagcaa tccccagaaa tgccagatta cgtatatcct ggcagcgatc 7560

7501 attgtcccac aatattcgtt aggggtcttt acggtctaat gcatatagga ccgtcgctag 7560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gctattttcc atgagtgaac gaacctggtc gaaatcagtg cgttcgaacg ctagagcctg 7620

7561 cgataaaagg tactcacttg cttggaccag ctttagtcac gcaagcttgc gatctcggac 7620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 ttttgcacgt tcaccggcat caacgttttc ttttcggatc cgccgcataa ccagtgaaac 7680

7621 aaaacgtgca agtggccgta gttgcaaaag aaaagcctag gcggcgtatt ggtcactttg 7680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 agcattgctg tcacttggtc gtggcagccc ggaccgacga tgaagcatgt ttagctggcc 7740

7681 tcgtaacgac agtgaaccag caccgtcggg cctggctgct acttcgtaca aatcgaccgg 7740

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 caaatgttgc tggatagttt ttactgccag accgcgcgcc tgaagatata gaagataatc 7800

7741 gtttacaacg acctatcaaa aatgacggtc tggcgcgcgg acttctatat cttctattag 7800

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 gcgaacatct tcaggttctg cgggaaacca tttccggtta ttcaacttgc accatgccgc 7860

7801 cgcttgtaga agtccaagac gccctttggt aaaggccaat aagttgaacg tggtacggcg 7860

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ccacgaccgg caaacggaca gaagcatttt ccaggtatgc tcagaaaacg cctggcgatc 7920

7861 ggtgctggcc gtttgcctgt cttcgtaaaa ggtccatacg agtcttttgc ggaccgctag 7920

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 cctgaacatg tccatcaggt tcttgcgaac ctcatcactc gttgcatcga ccggtaactt 7980

7921 ggacttgtac aggtagtcca agaacgcttg gagtagtgag caacgtagct ggccattgaa 7980

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ttagattaac atttgtttaa atatgaaatt aggcaatgta acatactgca ggcaaatttt 8040

7981 aatctaattg taaacaaatt tatactttaa tccgttacat tgtatgacgt ccgtttaaaa 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ggtgtacggt aagtaaattg gacatatgat ttaacaaagc gactataagt cagaaagtga 8100

8041 ccacatgcca ttcatttaac ctgtatacta aattgtttcg ctgatattca gtctttcact 8100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 gaatgagatt gaaataatta attcaactta ttcaattgat gatatgccag gattcctctt 8160

8101 cttactctaa ctttattaat taagttgaat aagttaacta ctatacggtc ctaaggagaa 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 cctttatata tcttcaatgt ttcgattaac aggtccatta tccggttttt agtttcgctg 8220

8161 ggaaatatat agaagttaca aagctaattg tccaggtaat aggccaaaaa tcaaagcgac 8220

.......... .......... .......... .......... .......... ..........

.......... .......... ........ HindIII(8252) ........ ..........

8221 cacattgccg aatgacaatc ggattccttt aagctttgct tttgccctgt tttctctccc 8280

8221 gtgtaacggc ttactgttag cctaaggaaa ttcgaaacga aaacgggaca aaagagaggg 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 cagtaatcgt tgggtgactg aaccatccag tagccgagag ttacgttttt cctgatttaa 8340

8281 gtcattagca acccactgac ttggtaggtc atcggctctc aatgcaaaaa ggactaaatt 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 taaatatcca tatctatttg tctcgtggtg ttttacagca actaaccgaa attactattt 8400

8341 atttataggt atagataaac agagcaccac aaaatgtcgt tgattggctt taatgataaa 8400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 tgcatcatcc aaccataaag agacaacaat aattctagaa aacatataac aagtgttttt 8460

8401 acgtagtagg ttggtatttc tctgttgtta ttaagatctt ttgtatattg ttcacaaaaa 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ttctatttac gtctcatacg caaagtccac tttaccatgt tttggaaact tgtacccaac 8520

8461 aagataaatg cagagtatgc gtttcaggtg aaatggtaca aaacctttga acatgggttg 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 cattcatttt tcgattttgt aatgaggctc ttttcttcta acaatcattg aggtcattat 8580

8521 gtaagtaaaa agctaaaaca ttactccgag aaaagaagat tgttagtaac tccagtaata 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 catgtgtcct cacctattgt ttaaattgag gcttgcgcta ttcccaaaat tgtgggtatt 8640

8581 gtacacagga gtggataaca aatttaactc cgaacgcgat aagggtttta acacccataa 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... KpnI(8693)

8641 attttcatat atcagtatct gcaatttcgg ttcaactccg gaagctcggt acccaactgt 8700

8641 taaaagtata tagtcataga cgttaaagcc aagttgaggc cttcgagcca tgggttgaca 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt 8760

8701 cccttatgag tccatagcat tctacgttct caagcttaga gaatcgttgg taataaaaaa 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 ttctctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact 8820

8761 aagagagttg tattgctctt gtgtgtcccc gcgatagcgt gtcttagttt aagctactga 8820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat 8880

8821 cctttaaaaa acaattaaag tctccagcgg actgcgtata tggaaaaagt tgacttttta 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg 8940

8881 accctctttt tcctttccac tctcgcggcc ttggccgaaa agtatatctt atctcttcgc 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat 9000

8941 aagtactgat ttacgaacgt agtgttatga acttcaactg ttataataaa ttcctggata 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca 9060

9001 acaaaaaagg ttatccacca atcgttagca gaatgaaaga ttgaaaagaa tggaaaatgt 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 tttcagcaat atatatatat atatatatat atatttcaag gatataccat tgtaatgtct 9120

9061 aaagtcgtta tatatatata tatatatata tataaagttc ctatatggta acattacaga 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa 9180

9121 cggggattct tctagcagca aaacggtcca ctggtgcaac cagttcttta gtgtcggctt 9180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa 9240

9181 cggtaattcc aagaatttcg ataaagacta caagcaaggt tacagttcaa gctaaagctt 9240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 aatcatttaa ttggtggtgc tgctatcgat gctacaggtg ttccacttcc agatgaggcg 9300

9241 ttagtaaatt aaccaccacg acgatagcta cgatgtccac aaggtgaagg tctactccgc 9300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 ctggaagcct ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg 9360

9301 gaccttcgga ggttcttccg actacggcaa aacaatccac gacacccacc aggatttacc 9360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg 9420

9361 ccatggccat cacaatctgg acttgttcca aatgattttt aggcatttct tgaagttaac 9420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 tacgccaact taagaccatg taactttgca tccgactctc ttttagactt atctccaatc 9480

9421 atgcggttga attctggtac attgaaacgt aggctgagag aaaatctgaa tagaggttag 9480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 aagccacaat ttgctaaagg tactgacttc gttgttgtta gagaattagt gggaggtatt 9540

9481 ttcggtgtta aacgatttcc atgactgaag caacaacaat ctcttaatca ccctccataa 9540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac 9600

9541 atgaaaccat tctctttcct tctgctacca ctaccacagc gaaccctatc acttgttatg 9600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag 9660

9601 tggcaaggtc ttcacgtttc ttagtgttct taccggcgaa agtaccggga tgttgtactc 9660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg 9720

9661 ggtggtaacg gataaaccag gaacctattt cgattacaaa accggagaag ttctaatacc 9720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 agaaaaactg tggaggaaac catcaagaac gaatttccta cattgaaagt tcaacatcaa 9780

9721 tctttttgac acctcctttg gtagttcttg cttaaaggat gtaactttca agttgtagtt 9780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata 9840

9781 aactaactaa gacggcggta ctaggatcaa ttcttgggtt gggtggattt accataatat 9840

.......... .......... ORF 2 .......... .......... ..........

.......... ......... EcoRV(9865) ......... .......... ..........

9841 atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggctcc 9900

9841 tagtggtcgt tgtacaaacc actatagtag aggctacttc ggaggcaata gggtccgagg 9900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt 9960

9901 aacccaaaca acggtagacg caggaaccgg agaaacggtc tgttcttgtg gcgtaaacca 9960

.......... .......... ORF 2 .......... .......... ..........

.......... NcoI(9976) .......... .......... .......... ..........

9961 ttgtacgaac catgccatgg ttccgctcca gatttgccaa agaataaggt caaccctatc 10020

9961 aacatgcttg gtacggtacc aaggcgaggt ctaaacggtt tcttattcca gttgggatag 10020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt 10080

10021 cggtgataga acagacgacg ttactacaac tttaacagta acttgaacgg acttcttcca 10080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 aaagccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta 10140

10081 tttcggtaac ttctacgtca atttttccaa aacctacgtc catagtcttg accactaaat 10140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc 10200

10141 ccaccaaggt tgtcatggtg gcttcagcca ctacgacagc ggcttcttca attcttttag 10200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 cttgcttaaa aagattctct ttttttgtga tatttgtaca taaactttat aaatgaaatt 10260

10201 gaacgaattt ttctaagaga aaaaaacact ataaacatgt atttgaaata tttactttaa 10260

ORF 2 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat 10320

10261 gtattatctt tgctgtgctt taatgtttta ccttatacaa gtatcccatc tgctttgata 10320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg 10380

10321 tatgcgttag atgtatgtaa atagttcttc ctctttttcc tcctacattt ccttatgtcc 10380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta 10440

10381 attcgtttaa ctatgattac cgagttgcac tattcctttt tcttaacgtg aaattgtaat 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 atattgacaa ggaggagggc atcaca.... .......... .......... .......... 10466

10441 tataactgtt cctcctcccg tagtgt.... .......... .......... .......... 10466

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-10466 Cross-reference: DOI; 10.1016/j.gene.2007.09.024. Cross-reference: PUBMED; 18054176. Reference author: Watson A.T., Garcia V., Bone N., Carr A.M., Armstrong J.; Reference title: "Gene tagging and gene replacement using recombinase-mediated cassette Reference title: exchange in Schizosaccharomyces pombe"; Reference location: Gene 407(1-2):63-74(2008).
Reference number: [2] Reference positions: 1-10466 Reference author: Watson A.T.; Reference title: ; Reference location: Submitted (27-JUN-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, Reference location: East Sussex BN1 9RQ, UK