Sequence of pAW8

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Sequence of pAW8_mCherry

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Sequence of pAW8_mCherry: 10622 BP; 2948 A; 2352 C; 2291 G; 3031 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 60

1 gtttttcaat ccacattgtc ttttagtact ttgatactaa agattaaata tataacctcc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 120

61 taaaagagat tttttttttt atgttgttta ttttttgtga gttactggac tggtaaacta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 180

121 cctcaaattc agttatggaa gaacttggta aagggtatta ccactttcaa gggagttctt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 240

181 aaaatgagac agtctttgcc ggaattgctg catcagctag gcctgctacc ggttggaagg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 300

241 ttaagtaatt tagctcagga ggtagtctac taaatgttcc aaaacgtatc ttagggattc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 360

301 attctcttca acaatcacta aagaagttca aatgatttta aatgtatgtt tacctactag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 420

361 agaagaggta acaaaggaaa gaagaacgtt tgcgcaaaat gtttctctac tgtacgataa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 480

421 aagactaata aaaaaagata tcaaaagata aaacatgcag gaaccctcag gctttacatt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 540

481 ttagccccat accaatcatc gccgtcgcaa gtaataccat atattttcta tttttcatcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 600

541 ccgttcgctc tgtcttaccc tatgttcccg tagcagatat taatatcgat ttttaacata 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 660

601 aaattaaaca taaaaaacat taaaataaag agtaacaaaa tgaattttac ttacgcttaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 720

661 tctcttttga agttgcttta cagttttatt cgagtcgttt tatatgttaa aatccctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 780

721 gctagtcgtt gaataagagg ccacatctaa gaaaaagaag tatttaaggt tgttacattg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 840

781 acggttaact aaaaagagat ctacgattat ttaactttga ttacttatat tattgtgtcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 900

841 aaaactattt aacatttgag aatgagtttt tttcttacaa ttttgattaa attggaaaaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 960

901 tatttccgag taccgcaatt tctcctatca tttttaagat gttaaaaaat taaattacag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ScaI(1014)

961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1020

961 tgttaacaga acgcttatat taagataatt aaaagcgatt gacgacaaaa tcatgataat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1080

1021 gaaagttata cggaatatac gttgaaatta agtacttctt acgttataaa aacgttagaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1140

1081 agaacatgac tcgcgaaaaa tagtaaatca tgagtaataa attaaaaagt catttcccgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1200

1141 tatttattta aacatcttct acgttacatt agaggagata ggaaaacgag tatacaaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1260

1201 ctcatatgga tcagatcttt ccgaacataa attttataat aagttatttt taagtgttaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1320

1261 aaatgctgta cacgatggta agtgaattga aggactaata ttttaaccaa gcaaatatga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1380

1321 ttaatgaatt catggaaatt gatttgtttt acggatatat atataattaa atgttactca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(1429) ..........

1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1440

1381 cagtctattc agtgatacag gctcaccaat tcctcaatct gagcggtagc taccatacca 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1500

1441 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct gtgggcggtt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1560

1501 gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg tctgttcgac 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1620

1561 actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct 1620

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1680

1621 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1740

1681 gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1800

1741 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1800

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1860

1801 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1860

.......... .......... ....... Ampicillin ORF 3 ....... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1920

1861 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1920

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1980

1921 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1980

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2040

1981 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2040

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2100

2041 atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg 2100

Ampicillin .......... ORF 3 .......... .......... ..........

.......... .......... ScaI(2128) .......... .......... ..........

2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2160

2101 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2220

2161 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2280

2221 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2340

2281 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2400

2341 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 2400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2460

2401 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2520

2461 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2580

2521 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2640

2581 gggcatagca tcaatagatg tgctgcccct cagtccggtg atacctactt gctttatctg 2640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2700

2641 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2760

2701 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2820

2761 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2880

2821 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2880

.......... .......... .......... pBR322_ori .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2940

2881 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2940

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3000

2941 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacaag 3000

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3060

3001 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 3060

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3120

3061 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 3120

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3180

3121 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 3180

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3240

3181 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 3240

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3300

3241 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 3300

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3360

3301 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 3360

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3420

3361 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 3420

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3480

3421 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3480

.......... pBR322_ori .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3540

3481 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3600

3541 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3660

3601 gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3720

3661 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3780

3721 tgcgttaatt acactcaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 3780

.......... .......... .......... .......... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3840

3781 gccgagcata caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac 3840

lac_promoter ........ ...... M13_pUC_rev_primer .. M13_reverse_primer

........ HindIII(3856) ........ .......... .......... ..........

3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3900

3841 tggtactaat gcggttcgaa cgtaccgccg gcgtattgaa gcatatcgta tgtaatatgc 3900

.......... .......... ORF 1 .......... loxP ..........

.......... .......... .......... .......... .......... ..........

3901 aagttatgca tgcttatggt gagcaagggc gaggaggata acatggccat catcaaggag 3960

3901 ttcaatacgt acgaatacca ctcgttcccg ctcctcctat tgtaccggta gtagttcctc 3960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 4020

3961 aagtacgcga agttccacgt gtacctcccg aggcacttgc cggtgctcaa gctctagctc 4020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 4080

4021 ccgctcccgc tcccggcggg gatgctcccg tgggtctggc ggttcgactt ccactggttc 4080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 4140

4081 ccaccggggg acgggaagcg gaccctgtag gacaggggag tcaagtacat gccgaggttc 4140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 4200

4141 cggatgcact tcgtggggcg gctgtagggg ctgatgaact tcgacaggaa ggggctcccg 4200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 4260

4201 aagttcaccc tcgcgcacta cttgaagctc ctgccgccgc accactggca ctgggtcctg 4260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 4320

4261 aggagggacg tcctgccgct caagtagatg ttccacttcg acgcgccgtg gttgaagggg 4320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... NcoI(4350) .......... .......... ..........

4321 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg 4380

4321 aggctgccgg ggcattacgt cttcttctgg tacccgaccc tccggaggag gctcgcctac 4380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 4440

4381 atggggctcc tgccgcggga cttcccgctc tagttcgtct ccgacttcga cttcctgccg 4440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 4500

4441 ccggtgatgc tgcgactcca gttctggtgg atgttccggt tcttcgggca cgtcgacggg 4500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 4560

4501 ccgcggatgt tgcagttgta gttcaacctg tagtggaggg tgttgctcct gatgtggtag 4560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 4620

4561 caccttgtca tgcttgcgcg gctcccggcg gtgaggtggc cgccgtacct gctcgacatg 4620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aagtaaggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta 4680

4621 ttcattccgc gcggtgaaga tttattcgct taaagaatac taaatactaa aaataataat 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac 4740

4681 ttattcaata ttttttttat tcacatatgt ttaaaatttc actgagaatc caaaattttg 4740

.......... .......... ...... pHybLex_rev_primer ...... ..........

.......... .......... .......... .......... .......... ..........

4741 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt 4800

4741 cttttaagaa taagaactca ttgagaaagg acatccagtc caacgaaaga gtccatatca 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa 4860

4801 tactccagcg agaataactg gtgtggagat ggccgtctag gcgatcccta ttgtcccatt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tatagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc 4920

4861 atatctagac aaatcgaacg gagcaggggc ggcccagtgg gccggtcgct gtacctccgg 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc 4980

4921 gtcttatggg aggaactgtc agaactgcac gcgtcgagtc cccgtactac actgacagcg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc 5040

4981 ggcatgtaaa tcgggtatgt aggggtacat attagtaaac gtaggtatgt aaaactaccg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct 5100

5041 gcgtgccgcg cttcgttttt aatgccgagg agcgacgtct ggacgctcgt ccctttgcga 5100

.......... .......... .......... .......... .......... ..........

.......... MluI(5112) .......... .......... .......... ..........

5101 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt 5160

5101 ggggagtgtc tgcgcaactt aacaggggtg cggcgcgggg acatctcttt atattttcca 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac 5220

5161 atcctaaacg gtgactccaa gaagaaagta tatgaaggaa aattttagaa cgatcctatg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 agttctcaca tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga 5280

5221 tcaagagtgt agtgtaggct tgtatttgtt ggtacccatt ccttttctga gtgcaaagct 5280

.......... .......... .......... .......... kanR ..........

.......... .......... .......... .......... .......... ..........

5281 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 5340

5281 ccggcgctaa tttaaggttg tacctacgac taaatatacc catatttacc cgagcgctat 5340

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt 5400

5341 tacagcccgt tagtccacgc tgttagatag ctaacatacc cttcgggcta cgcggtctca 5400

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 5460

5401 acaaagactt tgtaccgttt ccatcgcaac ggttactaca atgtctactc taccagtctg 5460

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 5520

5461 atttgaccga ctgccttaaa tacggagaag gctggtagtt cgtaaaatag gcatgaggac 5520

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag 5580

5521 tactacgtac caatgagtgg tgacgctagg ggccgttttg tcgtaaggtc cataatcttc 5580

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 5640

5581 ttataggact aagtccactt ttataacaac tacgcgaccg tcacaaggac gcggccaacg 5640

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 5700

5641 taagctaagg acaaacatta acaggaaaat tgtcgctagc gcataaagca gagcgagtcc 5700

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 5760

5701 gcgttagtgc ttacttattg ccaaaccaac tacgctcact aaaactactg ctcgcattac 5760

.......... .......... kanR .......... .......... ..........

.......... .......... ........ HindIII(5797) ........ ..........

5761 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt 5820

5761 cgaccggaca acttgttcag acctttcttt acgtattcga aaacggtaag agtggcctaa 5820

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 5880

5821 gtcagcagtg agtaccacta aagagtgaac tattggaata aaaactgctc ccctttaatt 5880

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 5940

5881 atccaacata actacaacct gctcagcctt agcgtctggc tatggtccta gaacggtagg 5940

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 6000

5941 ataccttgac ggagccactc aaaagaggaa gtaatgtctt tgccgaaaaa gtttttatac 6000

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 6060

6001 cataactatt aggactatac ttatttaacg tcaaagtaaa ctacgagcta ctcaaaaaga 6060

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt 6120

6061 ttagtcatga ctgttatttt tctaagaaca aaagttcttg aacagtaaac atatcaaaaa 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct 6180

6121 aatataacat caacaagata aaattagttt acaatcgcac taaatataaa aaaaagcgga 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg 6240

6181 gctgtagtag acgggtctac gcttcaattc acgcgtcttt cattatagta cgcagttagc 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtcga 6300

6241 atacacttac gaccagcgat atgacgacag ctaagctatg attgcggcgg taggtcagct 6300

.......... .......... .......... .......... .......... ..........

SpeI(6305) .......... .......... .... NotI(6346),EagI(6346) ....

6301 cgaactagta taacttcgta tatggtatta tatacgaagt tatgcggccg cgcgaattca 6360

6301 gcttgatcat attgaagcat ataccataat atatgcttca atacgccggc gcgcttaagt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 6420

6361 gaccggcagc aaaatgttgc agcactgacc cttttgggac cgcaatgggt tgaattagcg 6420

M13_forward20_primer M13_pUC_fwd_primer .. .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 6480

6421 gaacgtcgtg tagggggaaa gcggtcgacc gcattatcgc ttctccgggc gtggctagcg 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt 6540

6481 ggaagggttg tcaacgcgtc ggacttaccg cttaccgcgg actacgccat aaaagaggaa 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 acgcatctgt gcggtatttc acaccgcata ccatcgtccg gatcaatgaa gtagatgagt 6600

6541 tgcgtagaca cgccataaag tgtggcgtat ggtagcaggc ctagttactt catctactca 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ttactacctg tatatacttt tttttttgtc aaagtcactt ttatggcatt tccatgttga 6660

6601 aatgatggac atatatgaaa aaaaaaacag tttcagtgaa aataccgtaa aggtacaact 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 aggattagcc acagcactaa attttcagcg tatgattgct tttaaatatt taattttcat 6720

6661 tcctaatcgg tgtcgtgatt taaaagtcgc atactaacga aaatttataa attaaaagta 6720

.......... .......... .......... .......... .......... ..........

.......... PacI(6732) .......... .......... .......... ..........

6721 cgttttttaa ttaatattca aaacgattta atgccttcta catctgaagt caagctcata 6780

6721 gcaaaaaatt aattataagt tttgctaaat tacggaagat gtagacttca gttcgagtat 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gactcgggtt gggtagctaa aactacatct aacacgaagg ggtatagcct tcttaaagta 6840

6781 ctgagcccaa cccatcgatt ttgatgtaga ttgtgcttcc ccatatcgga agaatttcat 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 tactcatgta taaacgtttt gggttcatat ttttgggcca cgtggtttat aaaaattctt 6900

6841 atgagtacat atttgcaaaa cccaagtata aaaacccggt gcaccaaata tttttaagaa 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 aactacacca ctcggtatcc cgcacccgtc tacgtttcta cgatttcgag ctaatattga 6960

6901 ttgatgtggt gagccatagg gcgtgggcag atgcaaagat gctaaagctc gattataact 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gtaaagtgat tagcaaaaaa aataaaagta ctcgttgtcg gagatcaaga atttttctat 7020

6961 catttcacta atcgtttttt ttattttcat gagcaacagc ctctagttct taaaaagata 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tatctcatct aaaccacttt ctaaaagcga aaaacaaaat cgtaatatgc agcttgaatg 7080

7021 atagagtaga tttggtgaaa gattttcgct ttttgtttta gcattatacg tcgaacttac 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ggcttccata gtttgaaaga aaaaccctag cagtactggc aagggagaca ttccttttac 7140

7081 ccgaaggtat caaactttct ttttgggatc gtcatgaccg ttccctctgt aaggaaaatg 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ctggctaatc gccatcttcc agcaggcgca ccattgcccc tgtttcacta tccaggttac 7200

7141 gaccgattag cggtagaagg tcgtccgcgt ggtaacgggg acaaagtgat aggtccaatg 7200

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ggatatagtt catgacaata tttacattgg tccagccacc agcttgcatg atctccggta 7260

7201 cctatatcaa gtactgttat aaatgtaacc aggtcggtgg tcgaacgtac tagaggccat 7260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ttgaaactcc agcgcgggcc atatctcgcg cggctccgac acgggcactg tgtccagacc 7320

7261 aactttgagg tcgcgcccgg tatagagcgc gccgaggctg tgcccgtgac acaggtctgg 7320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 aggccaggta tctctgacca gagtcatcct tagcgccgta aatcaatcga tgagttgctt 7380

7321 tccggtccat agagactggt ctcagtagga atcgcggcat ttagttagct actcaacgaa 7380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 caaaaatccc ttccagggcg cgagttgata gctggctggt ggcagatggc gcggcaacac 7440

7381 gtttttaggg aaggtcccgc gctcaactat cgaccgacca ccgtctaccg cgccgttgtg 7440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cattttttct gacccggcaa aacaggtagt tattcggatc atcagctaca ccagagacgg 7500

7441 gtaaaaaaga ctgggccgtt ttgtccatca ataagcctag tagtcgatgt ggtctctgcc 7500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 aaatccatcg ctcgaccagt ttagttaccc ccaggctaag tgccttctct acacctgcgg 7560

7501 tttaggtagc gagctggtca aatcaatggg ggtccgattc acggaagaga tgtggacgcc 7560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 tgctaaccag cgttttcgtt ctgccaatat ggattaacat tctcccaccg tcagtacgtg 7620

7561 acgattggtc gcaaaagcaa gacggttata cctaattgta agagggtggc agtcatgcac 7620

.......... .......... ORF 1 .......... .......... ..........

EcoRV(7625) ........ .......... .......... .......... ..........

7621 agatatcttt aaccctgatc ctggcaattt cggctatacg taacagggtg ttataagcaa 7680

7621 tctatagaaa ttgggactag gaccgttaaa gccgatatgc attgtcccac aatattcgtt 7680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tccccagaaa tgccagatta cgtatatcct ggcagcgatc gctattttcc atgagtgaac 7740

7681 aggggtcttt acggtctaat gcatatagga ccgtcgctag cgataaaagg tactcacttg 7740

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gaacctggtc gaaatcagtg cgttcgaacg ctagagcctg ttttgcacgt tcaccggcat 7800

7741 cttggaccag ctttagtcac gcaagcttgc gatctcggac aaaacgtgca agtggccgta 7800

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 caacgttttc ttttcggatc cgccgcataa ccagtgaaac agcattgctg tcacttggtc 7860

7801 gttgcaaaag aaaagcctag gcggcgtatt ggtcactttg tcgtaacgac agtgaaccag 7860

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 gtggcagccc ggaccgacga tgaagcatgt ttagctggcc caaatgttgc tggatagttt 7920

7861 caccgtcggg cctggctgct acttcgtaca aatcgaccgg gtttacaacg acctatcaaa 7920

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ttactgccag accgcgcgcc tgaagatata gaagataatc gcgaacatct tcaggttctg 7980

7921 aatgacggtc tggcgcgcgg acttctatat cttctattag cgcttgtaga agtccaagac 7980

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 cgggaaacca tttccggtta ttcaacttgc accatgccgc ccacgaccgg caaacggaca 8040

7981 gccctttggt aaaggccaat aagttgaacg tggtacggcg ggtgctggcc gtttgcctgt 8040

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gaagcatttt ccaggtatgc tcagaaaacg cctggcgatc cctgaacatg tccatcaggt 8100

8041 cttcgtaaaa ggtccatacg agtcttttgc ggaccgctag ggacttgtac aggtagtcca 8100

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 tcttgcgaac ctcatcactc gttgcatcga ccggtaactt ttagattaac atttgtttaa 8160

8101 agaacgcttg gagtagtgag caacgtagct ggccattgaa aatctaattg taaacaaatt 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 atatgaaatt aggcaatgta acatactgca ggcaaatttt ggtgtacggt aagtaaattg 8220

8161 tatactttaa tccgttacat tgtatgacgt ccgtttaaaa ccacatgcca ttcatttaac 8220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 gacatatgat ttaacaaagc gactataagt cagaaagtga gaatgagatt gaaataatta 8280

8221 ctgtatacta aattgtttcg ctgatattca gtctttcact cttactctaa ctttattaat 8280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 attcaactta ttcaattgat gatatgccag gattcctctt cctttatctt caatgtttcg 8340

8281 taagttgaat aagttaacta ctatacggtc ctaaggagaa ggaaatagaa gttacaaagc 8340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 attaacaggt ccattatccg gtttttagtt tcgctgcaca ttgccgaatg acaatcggat 8400

8341 taattgtcca ggtaataggc caaaaatcaa agcgacgtgt aacggcttac tgttagccta 8400

.......... .......... .......... .......... .......... ..........

HindIII(8408) ...... .......... .......... .......... ..........

8401 tcctttaagc tttgcttttg ccctgttttc tctccccagt aatcgttggg tgactgaacc 8460

8401 aggaaattcg aaacgaaaac gggacaaaag agaggggtca ttagcaaccc actgacttgg 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 atccagtagc cgagagttac gtttttcctg atttaataaa tatccatatc tatttgtctc 8520

8461 taggtcatcg gctctcaatg caaaaaggac taaattattt ataggtatag ataaacagag 8520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 gtggtgtttt acagcaacta accgaaatta ctattttgca tcatccaacc ataaagagac 8580

8521 caccacaaaa tgtcgttgat tggctttaat gataaaacgt agtaggttgg tatttctctg 8580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 aacaataatt ctagaaaaca tataacaagt gtttttttct atttacgtct catacgcaaa 8640

8581 ttgttattaa gatcttttgt atattgttca caaaaaaaga taaatgcaga gtatgcgttt 8640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 gtccacttta ccatgttttg gaaacttgta cccaaccatt catttttcga ttttgtaatg 8700

8641 caggtgaaat ggtacaaaac ctttgaacat gggttggtaa gtaaaaagct aaaacattac 8700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 aggctctttt cttctaacaa tcattgaggt cattatcatg tgtcctcacc tattgtttaa 8760

8701 tccgagaaaa gaagattgtt agtaactcca gtaatagtac acaggagtgg ataacaaatt 8760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 attgaggctt gcgctattcc caaaattgtg ggtattattt tcatatatca gtatctgcaa 8820

8761 taactccgaa cgcgataagg gttttaacac ccataataaa agtatatagt catagacgtt 8820

.......... .......... .......... .......... .......... ..........

.......... .......... KpnI(8849) .......... .......... ..........

8821 tttcggttca actccggaag ctcggtaccc aactgtggga atactcaggt atcgtaagat 8880

8821 aaagccaagt tgaggccttc gagccatggg ttgacaccct tatgagtcca tagcattcta 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 gcaagagttc gaatctctta gcaaccatta ttttttttct ctcaacataa cgagaacaca 8940

8881 cgttctcaag cttagagaat cgttggtaat aaaaaaaaga gagttgtatt gctcttgtgt 8940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 caggggcgct atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag 9000

8941 gtccccgcga tagcgtgtct tagtttaagc tactgacctt taaaaaacaa ttaaagtctc 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 gtcgcctgac gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag 9060

9001 cagcggactg cgtatatgga aaaagttgac tttttaaccc tctttttcct ttccactctc 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 cgccggaacc ggcttttcat atagaataga gaagcgttca tgactaaatg cttgcatcac 9120

9061 gcggccttgg ccgaaaagta tatcttatct cttcgcaagt actgatttac gaacgtagtg 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 aatacttgaa gttgacaata ttatttaagg acctattgtt ttttccaata ggtggttagc 9180

9121 ttatgaactt caactgttat aataaattcc tggataacaa aaaaggttat ccaccaatcg 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 aatcgtctta ctttctaact tttcttacct tttacatttc agcaatatat atatatatat 9240

9181 ttagcagaat gaaagattga aaagaatgga aaatgtaaag tcgttatata tatatatata 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 atatatatat ttcaaggata taccattgta atgtctgccc ctaagaagat cgtcgttttg 9300

9241 tatatatata aagttcctat atggtaacat tacagacggg gattcttcta gcagcaaaac 9300

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

9301 ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct taaagctatt 9360

9301 ggtccactgg tgcaaccagt tctttagtgt cggcttcggt aattccaaga atttcgataa 9360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg tggtgctgct 9420

9361 agactacaag caaggttaca gttcaagcta aagcttttag taaattaacc accacgacga 9420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa gaaggctgat 9480

9421 tagctacgat gtccacaagg tgaaggtcta ctccgcgacc ttcggaggtt cttccgacta 9480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 gccgttttgt taggtgctgt gggtggtcct aaatggggta ccggtagtgt tagacctgaa 9540

9481 cggcaaaaca atccacgaca cccaccagga tttaccccat ggccatcaca atctggactt 9540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag accatgtaac 9600

9541 gttccaaatg atttttaggc atttcttgaa gttaacatgc ggttgaattc tggtacattg 9600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc taaaggtact 9660

9601 aaacgtaggc tgagagaaaa tctgaataga ggttagttcg gtgttaaacg atttccatga 9660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 gacttcgttg ttgttagaga attagtggga ggtatttact ttggtaagag aaaggaagac 9720

9661 ctgaagcaac aacaatctct taatcaccct ccataaatga aaccattctc tttccttctg 9720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9721 gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt gcaaagaatc 9780

9721 ctaccactac cacagcgaac cctatcactt gttatgtggc aaggtcttca cgtttcttag 9780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9781 acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat ttggtccttg 9840

9781 tgttcttacc ggcgaaagta ccgggatgtt gtactcggtg gtaacggata aaccaggaac 9840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc 9900

9841 ctatttcgat tacaaaaccg gagaagttct aatacctctt tttgacacct cctttggtag 9900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 aagaacgaat ttcctacatt gaaagttcaa catcaattga ttgattctgc cgccatgatc 9960

9901 ttcttgctta aaggatgtaa ctttcaagtt gtagttaact aactaagacg gcggtactag 9960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat gtttggtgat 10020

9961 gatcaattct tgggttgggt ggatttacca taatattagt ggtcgttgta caaaccacta 10020

.......... .......... ORF 2 .......... .......... ..........

EcoRV(10021) ........ .......... .......... .......... ..........

10021 atcatctccg atgaagcctc cgttatccca ggctccttgg gtttgttgcc atctgcgtcc 10080

10021 tagtagaggc tacttcggag gcaatagggt ccgaggaacc caaacaacgg tagacgcagg 10080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... ......... NcoI(10132)

10081 ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg ccatggttcc 10140

10081 aaccggagaa acggtctgtt cttgtggcgt aaaccaaaca tgcttggtac ggtaccaagg 10140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc tgctgcaatg 10200

10141 cgaggtctaa acggtttctt attccagttg ggatagcggt gatagaacag acgacgttac 10200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 atgttgaaat tgtcattgaa cttgcctgaa gaaggtaaag ccattgaaga tgcagttaaa 10260

10201 tacaacttta acagtaactt gaacggactt cttccatttc ggtaacttct acgtcaattt 10260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag taccaccgaa 10320

10261 ttccaaaacc tacgtccata gtcttgacca ctaaatccac caaggttgtc atggtggctt 10320

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga ttctcttttt 10380

10321 cagccactac gacagcggct tcttcaattc ttttaggaac gaatttttct aagagaaaaa 10380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 ttgtgatatt tgtacataaa ctttataaat gaaattcata atagaaacga cacgaaatta 10440

10381 aacactataa acatgtattt gaaatattta ctttaagtat tatctttgct gtgctttaat 10440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc 10500

10441 gttttacctt atacaagtat cccatctgct ttgatatatg cgttagatgt atgtaaatag 10500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc 10560

10501 ttcttcctct ttttcctcct acatttcctt atgtccattc gtttaactat gattaccgag 10560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 aacgtgataa ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcatca 10620

10561 ttgcactatt cctttttctt aacgtgaaat tgtaattata actgttcctc ctcccgtagt 10620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 ca........ .......... .......... .......... .......... .......... 10622

10621 gt........ .......... .......... .......... .......... .......... 10622

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-10622 Cross-reference: DOI; 10.1016/j.gene.2007.09.024. Cross-reference: PUBMED; 18054176. Reference author: Watson A.T., Garcia V., Bone N., Carr A.M., Armstrong J.; Reference title: "Gene tagging and gene replacement using recombinase-mediated cassette Reference title: exchange in Schizosaccharomyces pombe"; Reference location: Gene 407(1-2):63-74(2008).
Reference number: [2] Reference positions: 1-10622 Reference author: Watson A.T.; Reference title: ; Reference location: Submitted (27-JUN-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, Reference location: East Sussex BN1 9RQ, UK