Sequence of pAW8

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Sequence of pAW8_tdTomato

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Sequence of pAW8_tdTomato: 11342 BP; 3099 A; 2604 C; 2503 G; 3136 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 60

1 gtttttcaat ccacattgtc ttttagtact ttgatactaa agattaaata tataacctcc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 120

61 taaaagagat tttttttttt atgttgttta ttttttgtga gttactggac tggtaaacta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 180

121 cctcaaattc agttatggaa gaacttggta aagggtatta ccactttcaa gggagttctt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 240

181 aaaatgagac agtctttgcc ggaattgctg catcagctag gcctgctacc ggttggaagg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 300

241 ttaagtaatt tagctcagga ggtagtctac taaatgttcc aaaacgtatc ttagggattc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 360

301 attctcttca acaatcacta aagaagttca aatgatttta aatgtatgtt tacctactag 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 420

361 agaagaggta acaaaggaaa gaagaacgtt tgcgcaaaat gtttctctac tgtacgataa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 480

421 aagactaata aaaaaagata tcaaaagata aaacatgcag gaaccctcag gctttacatt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 540

481 ttagccccat accaatcatc gccgtcgcaa gtaataccat atattttcta tttttcatcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 600

541 ccgttcgctc tgtcttaccc tatgttcccg tagcagatat taatatcgat ttttaacata 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 660

601 aaattaaaca taaaaaacat taaaataaag agtaacaaaa tgaattttac ttacgcttaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 720

661 tctcttttga agttgcttta cagttttatt cgagtcgttt tatatgttaa aatccctttc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 780

721 gctagtcgtt gaataagagg ccacatctaa gaaaaagaag tatttaaggt tgttacattg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 840

781 acggttaact aaaaagagat ctacgattat ttaactttga ttacttatat tattgtgtcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 900

841 aaaactattt aacatttgag aatgagtttt tttcttacaa ttttgattaa attggaaaaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 960

901 tatttccgag taccgcaatt tctcctatca tttttaagat gttaaaaaat taaattacag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1020

961 tgttaacaga acgcttatat taagataatt aaaagcgatt gacgacaaaa tcatgataat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1080

1021 gaaagttata cggaatatac gttgaaatta agtacttctt acgttataaa aacgttagaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ScaI(1111) .......... ..........

1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1140

1081 agaacatgac tcgcgaaaaa tagtaaatca tgagtaataa attaaaaagt catttcccgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1200

1141 tatttattta aacatcttct acgttacatt agaggagata ggaaaacgag tatacaaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1260

1201 ctcatatgga tcagatcttt ccgaacataa attttataat aagttatttt taagtgttaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1320

1261 aaatgctgta cacgatggta agtgaattga aggactaata ttttaaccaa gcaaatatga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1380

1321 ttaatgaatt catggaaatt gatttgtttt acggatatat atataattaa atgttactca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ClaI(1429) ..........

1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1440

1381 cagtctattc agtgatacag gctcaccaat tcctcaatct gagcggtagc taccatacca 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1500

1441 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct gtgggcggtt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1560

1501 gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg tctgttcgac 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1620

1561 actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct 1620

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1680

1621 ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat tattaccaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1740

1681 gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa acaaataaaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1800

1741 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1800

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1860

1801 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1860

.......... .......... ....... Ampicillin ORF 3 ....... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1920

1861 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1920

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1980

1921 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1980

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2040

1981 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2040

.......... ....... Ampicillin ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2100

2041 atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca gcggcgtatg 2100

Ampicillin .......... ORF 3 .......... .......... ..........

.......... .......... ScaI(2128) .......... .......... ..........

2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2160

2101 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2220

2161 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2280

2221 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2340

2281 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2400

2341 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 2400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2460

2401 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2520

2461 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2580

2521 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2640

2581 gggcatagca tcaatagatg tgctgcccct cagtccggtg atacctactt gctttatctg 2640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2700

2641 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2700

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2760

2701 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2820

2761 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2880

2821 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2880

.......... .......... .......... pBR322_ori .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2940

2881 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2940

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3000

2941 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacaag 3000

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3060

3001 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 3060

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3120

3061 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 3120

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3180

3121 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 3180

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3240

3181 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 3240

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3300

3241 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 3300

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3360

3301 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 3360

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3420

3361 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 3420

.......... .......... pBR322_ori .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3480

3421 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3480

.......... pBR322_ori .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3540

3481 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3600

3541 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3660

3601 gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3720

3661 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3780

3721 tgcgttaatt acactcaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 3780

.......... .......... .......... .......... ......... lac_promoter

.......... .......... .......... .......... .......... ..........

3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3840

3781 gccgagcata caacacacct taacactcgc ctattgttaa agtgtgtcct ttgtcgatac 3840

lac_promoter ........ ...... M13_pUC_rev_primer .. M13_reverse_primer

........ HindIII(3856) ........ .......... .......... ..........

3841 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3900

3841 tggtactaat gcggttcgaa cgtaccgccg gcgtattgaa gcatatcgta tgtaatatgc 3900

.......... .......... ORF 1 .......... loxP ..........

.......... .......... .......... .......... .......... ..........

3901 aagttatgca tgcttatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc 3960

3901 ttcaatacgt acgaatacca ctcgttcccg ctcctccagt agtttctcaa gtacgcgaag 3960

.......... ......... ORF 1 ORF 2 ORF 4 ......... ..........

.......... .......... .......... .......... .......... ..........

3961 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag 4020

3961 ttccacgcgt acctcccgag gtacttgccg gtgctcaagc tctagctccc gctcccgctc 4020

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg 4080

4021 ccggcgggga tgctcccgtg ggtctggcgg ttcgacttcc actggttccc gccgggggac 4080

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag 4140

4081 gggaagcgga ccctgtagga caggggggtc aagtacatgc cgaggttccg catgcacttc 4140

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag 4200

4141 gtggggcggc tgtaggggct aatgttcttc gacaggaagg ggctcccgaa gttcaccctc 4200

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag 4260

4201 gcgcactact tgaagctcct gccgccagac cactggcact gggtcctgag gagggacgtc 4260

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc 4320

4261 ctgccgtgcg actagatgtt ccacttctac gcgccgtggt tgaagggggg gctgccgggg 4320

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... NcoI(4338) .......... .......... .......... ..........

4321 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac 4380

4321 cattacgtct tcttctggta cccgaccctc cggaggtggc tcgcggacat gggggcgctg 4380

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg 4440

4381 ccgcacgact tcccgctcta ggtggtccgg gacttcgact tcctgccgcc ggtgatggac 4440

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac 4500

4441 cacctcaagt tctggtagat gtaccggttc ttcgggcacg ttgacgggcc gatgatgatg 4500

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 4560

4501 cacctgtggt tcgacctgta gtggagggtg ttgctcctga tgtggtagca ccttgtcatg 4560

.......... ...... ORF 1 ORF 2 ORF 4 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc 4620

4561 ctcgcgaggc tcccggcggt ggtggacaag gaccccgtac cgtggccgtc gtggccgtcg 4620

......... ORF 2 ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg 4680

4621 ccgtcgaggc cgtggcggag gaggctcctg ttgttgtacc ggcagtagtt tctcaagtac 4680

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag 4740

4681 gcgaagttcc acgcgtacct cccgaggtac ttgccggtgc tcaagctcta gctcccgctc 4740

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc 4800

4741 ccgctcccgg cggggatgct cccgtgggtc tggcggttcg acttccactg gttcccgccg 4800

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac 4860

4801 ggggacggga agcggaccct gtaggacagg ggggtcaagt acatgccgag gttccgcatg 4860

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag 4920

4861 cacttcgtgg ggcggctgta ggggctaatg ttcttcgaca ggaaggggct cccgaagttc 4920

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc 4980

4921 accctcgcgc actacttgaa gctcctgccg ccagaccact ggcactgggt cctgaggagg 4980

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac 5040

4981 gacgtcctgc cgtgcgacta gatgttccac ttctacgcgc cgtggttgaa gggggggctg 5040

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc 5100

5041 ccggggcatt acgtcttctt ctggtacccg accctccgga ggtggctcgc ggacatgggg 5100

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccgc 5160

5101 gcgctgccgc acgacttccc gctctaggtg gtccgggact tcgacttcct gccgccggcg 5160

.......... ......... ORF 1 ORF 4 ......... .......... ..........

SexAI(5163) ........ .......... .......... .......... ..........

5161 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac 5220

5161 atggaccacc tcaagttctg gtagatgtac cggttcttcg ggcacgttga cgggccgatg 5220

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa 5280

5221 atgatgcacc tgtggttcga cctgtagtgg agggtgttgc tcctgatgtg gtagcacctt 5280

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac 5340

5281 gtcatgctcg cgaggctccc ggcggtggtg gacaaggaca tgccgtacct gctcgacatg 5340

.......... ......... ORF 1 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aagtaaggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta 5400

5341 ttcattccgc gcggtgaaga tttattcgct taaagaatac taaatactaa aaataataat 5400

.......... ORF 4 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac 5460

5401 ttattcaata ttttttttat tcacatatgt ttaaaatttc actgagaatc caaaattttg 5460

.......... .......... ...... pHybLex_rev_primer ...... ..........

.......... .......... .......... .......... .......... ..........

5461 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt 5520

5461 cttttaagaa taagaactca ttgagaaagg acatccagtc caacgaaaga gtccatatca 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa 5580

5521 tactccagcg agaataactg gtgtggagat ggccgtctag gcgatcccta ttgtcccatt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tatagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc 5640

5581 atatctagac aaatcgaacg gagcaggggc ggcccagtgg gccggtcgct gtacctccgg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc 5700

5641 gtcttatggg aggaactgtc agaactgcac gcgtcgagtc cccgtactac actgacagcg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc 5760

5701 ggcatgtaaa tcgggtatgt aggggtacat attagtaaac gtaggtatgt aaaactaccg 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct 5820

5761 gcgtgccgcg cttcgttttt aatgccgagg agcgacgtct ggacgctcgt ccctttgcga 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt 5880

5821 ggggagtgtc tgcgcaactt aacaggggtg cggcgcgggg acatctcttt atattttcca 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac 5940

5881 atcctaaacg gtgactccaa gaagaaagta tatgaaggaa aattttagaa cgatcctatg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 agttctcaca tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga 6000

5941 tcaagagtgt agtgtaggct tgtatttgtt ggtacccatt ccttttctga gtgcaaagct 6000

.......... .......... .......... .......... kanR ..........

.......... .......... .......... .......... .......... ..........

6001 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 6060

6001 ccggcgctaa tttaaggttg tacctacgac taaatatacc catatttacc cgagcgctat 6060

.......... .......... kanR .......... .......... ..........

.......... .......... ClaI(6090) .......... .......... ..........

6061 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt 6120

6061 tacagcccgt tagtccacgc tgttagatag ctaacatacc cttcgggcta cgcggtctca 6120

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 6180

6121 acaaagactt tgtaccgttt ccatcgcaac ggttactaca atgtctactc taccagtctg 6180

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 6240

6181 atttgaccga ctgccttaaa tacggagaag gctggtagtt cgtaaaatag gcatgaggac 6240

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag 6300

6241 tactacgtac caatgagtgg tgacgctagg ggccgttttg tcgtaaggtc cataatcttc 6300

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 6360

6301 ttataggact aagtccactt ttataacaac tacgcgaccg tcacaaggac gcggccaacg 6360

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 6420

6361 taagctaagg acaaacatta acaggaaaat tgtcgctagc gcataaagca gagcgagtcc 6420

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 6480

6421 gcgttagtgc ttacttattg ccaaaccaac tacgctcact aaaactactg ctcgcattac 6480

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt 6540

6481 cgaccggaca acttgttcag acctttcttt acgtattcga aaacggtaag agtggcctaa 6540

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 6600

6541 gtcagcagtg agtaccacta aagagtgaac tattggaata aaaactgctc ccctttaatt 6600

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 6660

6601 atccaacata actacaacct gctcagcctt agcgtctggc tatggtccta gaacggtagg 6660

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 6720

6661 ataccttgac ggagccactc aaaagaggaa gtaatgtctt tgccgaaaaa gtttttatac 6720

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 6780

6721 cataactatt aggactatac ttatttaacg tcaaagtaaa ctacgagcta ctcaaaaaga 6780

.......... .......... kanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt 6840

6781 ttagtcatga ctgttatttt tctaagaaca aaagttcttg aacagtaaac atatcaaaaa 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct 6900

6841 aatataacat caacaagata aaattagttt acaatcgcac taaatataaa aaaaagcgga 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg 6960

6901 gctgtagtag acgggtctac gcttcaattc acgcgtcttt cattatagta cgcagttagc 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtcga 7020

6961 atacacttac gaccagcgat atgacgacag ctaagctatg attgcggcgg taggtcagct 7020

.......... .......... .......... .......... .......... ..........

SpeI(7025) .......... .......... .... EagI(7066),NotI(7066) ....

7021 cgaactagta taacttcgta tatggtatta tatacgaagt tatgcggccg cgcgaattca 7080

7021 gcttgatcat attgaagcat ataccataat atatgcttca atacgccggc gcgcttaagt 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 7140

7081 gaccggcagc aaaatgttgc agcactgacc cttttgggac cgcaatgggt tgaattagcg 7140

M13_forward20_primer M13_pUC_fwd_primer .. .......... ..........

.......... ......... PvuII(7167) ......... .......... ..........

7141 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 7200

7141 gaacgtcgtg tagggggaaa gcggtcgacc gcattatcgc ttctccgggc gtggctagcg 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt 7260

7201 ggaagggttg tcaacgcgtc ggacttaccg cttaccgcgg actacgccat aaaagaggaa 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 acgcatctgt gcggtatttc acaccgcata ccatcgtccg gatcaatgaa gtagatgagt 7320

7261 tgcgtagaca cgccataaag tgtggcgtat ggtagcaggc ctagttactt catctactca 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ttactacctg tatatacttt tttttttgtc aaagtcactt ttatggcatt tccatgttga 7380

7321 aatgatggac atatatgaaa aaaaaaacag tttcagtgaa aataccgtaa aggtacaact 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 aggattagcc acagcactaa attttcagcg tatgattgct tttaaatatt taattttcat 7440

7381 tcctaatcgg tgtcgtgatt taaaagtcgc atactaacga aaatttataa attaaaagta 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cgttttttaa ttaatattca aaacgattta atgccttcta catctgaagt caagctcata 7500

7441 gcaaaaaatt aattataagt tttgctaaat tacggaagat gtagacttca gttcgagtat 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 gactcgggtt gggtagctaa aactacatct aacacgaagg ggtatagcct tcttaaagta 7560

7501 ctgagcccaa cccatcgatt ttgatgtaga ttgtgcttcc ccatatcgga agaatttcat 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 tactcatgta taaacgtttt gggttcatat ttttgggcca cgtggtttat aaaaattctt 7620

7561 atgagtacat atttgcaaaa cccaagtata aaaacccggt gcaccaaata tttttaagaa 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 aactacacca ctcggtatcc cgcacccgtc tacgtttcta cgatttcgag ctaatattga 7680

7621 ttgatgtggt gagccatagg gcgtgggcag atgcaaagat gctaaagctc gattataact 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 gtaaagtgat tagcaaaaaa aataaaagta ctcgttgtcg gagatcaaga atttttctat 7740

7681 catttcacta atcgtttttt ttattttcat gagcaacagc ctctagttct taaaaagata 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 tatctcatct aaaccacttt ctaaaagcga aaaacaaaat cgtaatatgc agcttgaatg 7800

7741 atagagtaga tttggtgaaa gattttcgct ttttgtttta gcattatacg tcgaacttac 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ggcttccata gtttgaaaga aaaaccctag cagtactggc aagggagaca ttccttttac 7860

7801 ccgaaggtat caaactttct ttttgggatc gtcatgaccg ttccctctgt aaggaaaatg 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ctggctaatc gccatcttcc agcaggcgca ccattgcccc tgtttcacta tccaggttac 7920

7861 gaccgattag cggtagaagg tcgtccgcgt ggtaacgggg acaaagtgat aggtccaatg 7920

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ggatatagtt catgacaata tttacattgg tccagccacc agcttgcatg atctccggta 7980

7921 cctatatcaa gtactgttat aaatgtaacc aggtcggtgg tcgaacgtac tagaggccat 7980

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ttgaaactcc agcgcgggcc atatctcgcg cggctccgac acgggcactg tgtccagacc 8040

7981 aactttgagg tcgcgcccgg tatagagcgc gccgaggctg tgcccgtgac acaggtctgg 8040

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... ClaI(8088) ..........

8041 aggccaggta tctctgacca gagtcatcct tagcgccgta aatcaatcga tgagttgctt 8100

8041 tccggtccat agagactggt ctcagtagga atcgcggcat ttagttagct actcaacgaa 8100

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 caaaaatccc ttccagggcg cgagttgata gctggctggt ggcagatggc gcggcaacac 8160

8101 gtttttaggg aaggtcccgc gctcaactat cgaccgacca ccgtctaccg cgccgttgtg 8160

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 cattttttct gacccggcaa aacaggtagt tattcggatc atcagctaca ccagagacgg 8220

8161 gtaaaaaaga ctgggccgtt ttgtccatca ataagcctag tagtcgatgt ggtctctgcc 8220

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 aaatccatcg ctcgaccagt ttagttaccc ccaggctaag tgccttctct acacctgcgg 8280

8221 tttaggtagc gagctggtca aatcaatggg ggtccgattc acggaagaga tgtggacgcc 8280

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 tgctaaccag cgttttcgtt ctgccaatat ggattaacat tctcccaccg tcagtacgtg 8340

8281 acgattggtc gcaaaagcaa gacggttata cctaattgta agagggtggc agtcatgcac 8340

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 agatatcttt aaccctgatc ctggcaattt cggctatacg taacagggtg ttataagcaa 8400

8341 tctatagaaa ttgggactag gaccgttaaa gccgatatgc attgtcccac aatattcgtt 8400

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 tccccagaaa tgccagatta cgtatatcct ggcagcgatc gctattttcc atgagtgaac 8460

8401 aggggtcttt acggtctaat gcatatagga ccgtcgctag cgataaaagg tactcacttg 8460

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 gaacctggtc gaaatcagtg cgttcgaacg ctagagcctg ttttgcacgt tcaccggcat 8520

8461 cttggaccag ctttagtcac gcaagcttgc gatctcggac aaaacgtgca agtggccgta 8520

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 caacgttttc ttttcggatc cgccgcataa ccagtgaaac agcattgctg tcacttggtc 8580

8521 gttgcaaaag aaaagcctag gcggcgtatt ggtcactttg tcgtaacgac agtgaaccag 8580

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 gtggcagccc ggaccgacga tgaagcatgt ttagctggcc caaatgttgc tggatagttt 8640

8581 caccgtcggg cctggctgct acttcgtaca aatcgaccgg gtttacaacg acctatcaaa 8640

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 ttactgccag accgcgcgcc tgaagatata gaagataatc gcgaacatct tcaggttctg 8700

8641 aatgacggtc tggcgcgcgg acttctatat cttctattag cgcttgtaga agtccaagac 8700

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 cgggaaacca tttccggtta ttcaacttgc accatgccgc ccacgaccgg caaacggaca 8760

8701 gccctttggt aaaggccaat aagttgaacg tggtacggcg ggtgctggcc gtttgcctgt 8760

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 gaagcatttt ccaggtatgc tcagaaaacg cctggcgatc cctgaacatg tccatcaggt 8820

8761 cttcgtaaaa ggtccatacg agtcttttgc ggaccgctag ggacttgtac aggtagtcca 8820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 tcttgcgaac ctcatcactc gttgcatcga ccggtaactt ttagattaac atttgtttaa 8880

8821 agaacgcttg gagtagtgag caacgtagct ggccattgaa aatctaattg taaacaaatt 8880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 atatgaaatt aggcaatgta acatactgca ggcaaatttt ggtgtacggt aagtaaattg 8940

8881 tatactttaa tccgttacat tgtatgacgt ccgtttaaaa ccacatgcca ttcatttaac 8940

.......... .......... .......... .......... .......... ..........

NdeI(8945) .......... .......... .......... .......... ..........

8941 gacatatgat ttaacaaagc gactataagt cagaaagtga gaatgagatt gaaataatta 9000

8941 ctgtatacta aattgtttcg ctgatattca gtctttcact cttactctaa ctttattaat 9000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 attcaactta ttcaattgat gatatgccag gattcctctt cctttatctt caatgtttcg 9060

9001 taagttgaat aagttaacta ctatacggtc ctaaggagaa ggaaatagaa gttacaaagc 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 attaacaggt ccattatccg gtttttagtt tcgctgcaca ttgccgaatg acaatcggat 9120

9061 taattgtcca ggtaataggc caaaaatcaa agcgacgtgt aacggcttac tgttagccta 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 tcctttaagc tttgcttttg ccctgttttc tctccccagt aatcgttggg tgactgaacc 9180

9121 aggaaattcg aaacgaaaac gggacaaaag agaggggtca ttagcaaccc actgacttgg 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 atccagtagc cgagagttac gtttttcctg atttaataaa tatccatatc tatttgtctc 9240

9181 taggtcatcg gctctcaatg caaaaaggac taaattattt ataggtatag ataaacagag 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 gtggtgtttt acagcaacta accgaaatta ctattttgca tcatccaacc ataaagagac 9300

9241 caccacaaaa tgtcgttgat tggctttaat gataaaacgt agtaggttgg tatttctctg 9300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 aacaataatt ctagaaaaca tataacaagt gtttttttct atttacgtct catacgcaaa 9360

9301 ttgttattaa gatcttttgt atattgttca caaaaaaaga taaatgcaga gtatgcgttt 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 gtccacttta ccatgttttg gaaacttgta cccaaccatt catttttcga ttttgtaatg 9420

9361 caggtgaaat ggtacaaaac ctttgaacat gggttggtaa gtaaaaagct aaaacattac 9420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 aggctctttt cttctaacaa tcattgaggt cattatcatg tgtcctcacc tattgtttaa 9480

9421 tccgagaaaa gaagattgtt agtaactcca gtaatagtac acaggagtgg ataacaaatt 9480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 attgaggctt gcgctattcc caaaattgtg ggtattattt tcatatatca gtatctgcaa 9540

9481 taactccgaa cgcgataagg gttttaacac ccataataaa agtatatagt catagacgtt 9540

.......... .......... .......... .......... .......... ..........

.......... .......... KpnI(9569) .......... .......... ..........

9541 tttcggttca actccggaag ctcggtaccc aactgtggga atactcaggt atcgtaagat 9600

9541 aaagccaagt tgaggccttc gagccatggg ttgacaccct tatgagtcca tagcattcta 9600

.......... .......... .......... .......... .......... ..........

.......... .......... ..........