UBUBH1 CTTCAAGGAATTAATTCTATATTCTAAAAACACAAATGATACTTCTAAAA 51 AAAATCATGAATGGCATCAACTCTGAATCAAATCTTTGCAGATGCACCTA 101 CTTCTCATTTCCACTGTCACATCATTTTTCCAGATCTCGCTGCCTGTTAT 151 GTGGCCCACAAACCAAGACACGTTTTATGGCCATTAAAGCTGGCTGATCG 201 TCGCCAAACACCAAATACATATCAATATGTACATTCGAGAAAGAAGCGAT 251 CAAAGAAGCGTCTTCGGGCGAGTAGGAGAATGCGGAGGAGAAGGAGAACG 301 AGCTGATCTAGTATCTCTCCACAATCCAATGCCAACTGACCAACTGGCCA 351 TATTCGGAGCAATTTGAAGCCAATTTCCATCGCCTGGCGATCGCTCCATT 401 CTTGGCTATATGTTTTTCACCGTTCCCGGGGCCATTTTCAAAGACTCGTC 451 GGTAAGATAAGATTGTGTCACTCGCTGTCTCTCTTCATTTGTCGAAGAAT 501 GCTGAGGAATTTCGCGATGACGTCGGCGAGTATTTTGAAGAATGAGAATA 551 ATTTGTATTTATACGAAAATCAGTTAGTGGAATTTTCTACAAAAACATGT 601 TATCTATAGATAATTTTGTTGCAAAATATGTTGACTATGACAAAGATTGT 651 ATGTATATACCTTTAATGTATTCTCATTTTCTTATGTATTTATAATGGCA 701 ATGATGATACTGATGATATTTTAAGATGATGCCAGACCACAGGCTGATTT 751 CTGCGTCTTTTGCCGAACGCAGTGCATGTGCGGTTGTTGTTTTTTGGAAT 801 AGTTTCAATTTTCGGACTGTCCGCTTTGATTTCAGTTTCTTGGCTTATTC 851 AAAAAGCAAAGTAAAGCCAAAAAAGCGAGATGGCAATACCAAATGCGGCA 901 AAACGGTAGTGGAAGGAAAGGGGTGCGGGGCAGCGGAAGGAAGGGTGGGG 951 CGGGGCGTGGCGGGGTCTGTGGCTGGGCGCGACGTCACCGACGTTGGAGC 1001 CACTCCTTTGACCATGTGTGCGTGTGTGTATTATTCGTGTCTCGCCACTC 1051 GCCGGTTGTTTTTTTCTTTTTATCTCGCTCTCTCTAGCGCCATCTCGTAC 1101 GCATGCTCAACGCACCGCATGTTGCCGTGTCCTTTATGCGTCATTTTGGC 1151 TCGAAATAGGCAATTATTTAAACAAAGATTAGTCAACGAAAACGCTAAAA 1201 TAAATAAGTCTACAATATGGTTACTTATTGCCATGTGTGTGCAGCCAACG 1251 ATAGCAACAAAAGCAACAACACAGTGGCTTTCCCTCTTTCACTTTTTGTT 1301 TGCAAGCGCGTGCGAGCAAGACGGCACGACCGGCAAACGCAATTACGCTG 1351 ACAAAGAGCAGACGAAGTTTTGGCCGAAAAACATCAAGGCGCCTGATACG 1401 AATGCATTTGCAATAACAATTGCGATATTTAATATTGTTTATGAAGCTGT 1451 TTGACTTCAAAACACACAAAAAAAAAAATAAAACAAATTATTTGAAAGAG 1501 AATTAGGAATCGGACAGCTTATCGTTACGGGCTAACAGCACACCGAGACG 1551 AAATAGCTTACCTGACGTCACAGCCTCTGGAAGAACTGCCGCCAAGCAGA 1601 GAGAGAGAGAAAAAGAGGGAGAGCAGCTTAGACCGCATGTGCTTGTGTGT 1651 GAGGCGTCTCTCTCTTCGTCTCCTGTTTGCGCAAACGCATAGACTGCACT 1701 GAGAAAATCGATTACCTATTTTTTATGAATGAATATTTGCACTATTACTA 1751 TTCAAAACTATTAAGATAGCAATCACATTCAATAGCCAAATACTATACCA 1801 CCTGAGCGATGCAACGAAATGATCAATTTGAGCAAAAATGCTGCATATTT 1851 AGGACGGCATCATTATAGAAATGCTTCTTGCTGTGTACTTTTCTCTCGTC 1901 TGGCAGCTGTTTCGCCGTTATTGTTAAAACCGGCTTAAGTTAGGTGTGTT 1951 TTCTACGACTAGTGATGCCCCTACTAGAAGATGTGTGTTGCACAAATGTC 2001 CCTGAATAACCAATTTGAAGTGCAGATAGCAGTAAACGTAAGCTAATATG 2051 AATATTATTTAACTGTAATGTTTTAATATCGCTGGACATTACTAATAAAC 2101 CCACTATAAACACATGTACATATGTATGTTTTGGCATACAATGAGTAGTT 2151 GGGGAAAAAATGTGTAAAAGCACCGTGACCATCACAGCATAAAGATAACC 2201 AGCTGAAGTATCGAATATGAGTAACCCCCAAATTGAATCACATGCCGCAA 2251 CTGATAGGACCCATGGAAGTACACTCTTCATGGCGATATACAAGACACAC 2301 ACAAGCACGAACACCCAGTTGCGGAGGAAATTCTCCGTAAATGAAAACCC 2351 AATCGGCGAACAATTCATACCCATATATGGTAAAAGTTTTGAACGCGACT 2401 TGAGAGCGGAGAGCATTGCGGCTGATAAGGTTTTAGCGCTAAGCGGGCTT 2451 TATAAAACGGGCTGCGGGACCAGTTTTCATATCACTACCGTTTGAGTTCT 2501 TGTGCTGTGTGGATACTCCTCCCGACACAAAGCCGCTCCATCAGCCAGCA 2551 GTCGTCTAATCCAGAGACCCCGGATCGGGGTACCTACTAGTCCAGTGTGG 2601 TGGAATTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGTCTAGAGGG 2651 CCCTTCGAAGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTAC 2701 GCGTACCGGTCATCATCACCATCACCATTGAGTTTAAACCCGCTGATCAG 2751 CCTCGACTGTGCCTTCTAAGGCCTGAGCTCGCTGATCAGCCTCGATCGAG 2801 GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG 2851 AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT 2901 TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC 2951 ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG 3001 CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCAGTCGACCG 3051 ATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGG 3101 CATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCG 3151 TAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTT 3201 CGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTT 3251 GCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCG 3301 GCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTAC 3351 GTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGAT 3401 TCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGT 3451 CCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCG 3501 GCTCTTACCAGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT 3551 GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC 3601 GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG 3651 TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT 3701 TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC 3751 AATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG 3801 CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC 3851 CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC 3901 TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT 3951 GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC 4001 AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG 4051 TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT 4101 TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA 4151 TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC 4201 GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATC 4251 CTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTA 4301 AACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAG 4351 CGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAG 4401 ATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGAT 4451 ACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGC 4501 CAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCC 4551 ATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGT 4601 TAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCAC 4651 GCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGG 4701 CGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGG 4751 TCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGG 4801 TTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGC 4851 TTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTAT 4901 GCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGC 4951 CACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGG 5001 CGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACC 5051 CACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTT 5101 CTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGG 5151 GCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTG 5201 AAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTA 5251 TTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG 5301 CCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAA 5351 TAGGCGTATCACGAGGCCCTTTCGT pAc5.1/V5-His A "Times New RomanUBC Orf frame 3"Times New RomanUBC  AC5_primer"Times New RomanUBCɆd V5"Times New RomanUB 6xHis"Times New RomanUBC BGH_rev_primer"Times New RomanUBC  EBV_rev_primer"Times New RomanUBCɆ r SV40_PA_terminator"Times New RomanUB OFR3Times New Roman2UBC 6OFR3Times New RomanUBC  tet(1032-576)"Times New RomanUBCɆ A pBR322_origin"Times New RomanUB8 Orf frame 2"Times New RomanUBC8 Ampicillin"Times New RomanUBCb~ AmpR_promoter"Times New Roman UBBglII"Times New Roman VC/| / UBSmaI"Times New Roman lZH9H UBNarI"Times New Romanm @#T$g$g+ UBClaI"Times New Roman 1kCrUyUr UBAflII"Times New Roman &55t UBNdeI(2120),NcoI(2261)"Times New RomanH1R UBaKpnI(2583), EcoRI(2603), EcoRV(2615), NotI(2630), XhoI(2636), XbaI(2642), ApaI(2652), BstBI(2655)"Times New RomanL O G UB/AgeI(2705), StuI(2771), SacI(2779), BamHI(2800)"Times New Roman (gfy y G UBHpaI"Times New Romanw E@:Z UBSalI"Times New Roman .& UBtest"Times New Roman dgI**I