Sequence of pAc5.1_V5-His

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pAc5.1_V5-His_A

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pAc5.1/V5-His A : 5375 BP; 1459 A; 1254 C; 1230 G; 1432 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga 60

1 gaagttcctt aattaagata taagattttt gtgtttacta tgaagatttt ttttagtact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac 120

61 taccgtagtt gagacttagt ttagaaacgt ctacgtggat gaagagtaaa ggtgacagtg 120

.......... .......... .......... .......... .......... ..........

.......... BglII(132) .......... .......... .......... ..........

121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg 180

121 tagtaaaaag gtctagagcg acggacaata caccgggtgt ttggttctgt gcaaaatacc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga 240

181 ggtaatttcg accgactagc agcggtttgt ggtttatgta tagttataca tgtaagctct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg 300

241 ttcttcgcta gtttcttcgc agaagcccgc tcatcctctt acgcctcctc ttcctcttgc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc 360

301 tcgactagat catagagagg tgttaggtta cggttgactg gttgaccggt ataagcctcg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac 420

361 ttaaacttcg gttaaaggta gcggaccgct agcgaggtaa gaaccgatat acaaaaagtg 420

.......... .......... .......... .......... .......... ..........

SmaI(427) .......... .......... .......... .......... ..........

421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct 480

421 gcaagggccc cggtaaaagt ttctgagcag ccattctatt ctaacacagt gagcgacaga 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag 540

481 gagaagtaaa cagcttctta cgactcctta aagcgctact gcagccgctc ataaaacttc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt 600

541 ttactcttat taaacataaa tatgctttta gtcaatcacc ttaaaagatg tttttgtaca 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac 660

601 atagatatct attaaaacaa cgttttatac aactgatact gtttctaaca tacatatatg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt 720

661 gaaattacat aagagtaaaa gaatacataa atattaccgt tactactatg actactataa 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg 780

721 aattctacta cggtctggtg tccgactaaa gacgcagaaa acggcttgcg tcacgtacac 780

.......... .......... ......... Orf frame 3 ......... ..........

.......... .......... .......... .......... .......... ..........

781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct 840

781 gccaacaaca aaaaacctta tcaaagttaa aagcctgaca ggcgaaacta aagtcaaaga 840

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca 900

841 accgaataag tttttcgttt catttcggtt ttttcgctct accgttatgg tttacgccgt 900

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg 960

901 tttgccatca ccttcctttc cccacgcccc gtcgccttcc ttcccacccc gccccgcacc 960

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg 1020

961 gccccagaca ccgacccgcg ctgcagtggc tgcaacctcg gtgaggaaac tggtacacac 1020

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc 1080

1021 gcacacacat aataagcaca gagcggtgag cggccaacaa aaaaagaaaa atagagcgag 1080

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg 1140

1081 agagatcgcg gtagagcatg cgtacgagtt gcgtggcgta caacggcaca ggaaatacgc 1140

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa 1200

1141 agtaaaaccg agctttatcc gttaataaat ttgtttctaa tcagttgctt ttgcgatttt 1200

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa 1260

1201 atttattcag atgttatacc aatgaataac ggtacacaca cgtcggttgc tatcgttgtt 1260

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag 1320

1261 ttcgttgttg tgtcaccgaa agggagaaag tgaaaaacaa acgttcgcgc acgctcgttc 1320

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa 1380

1321 tgccgtgctg gccgtttgcg ttaatgcgac tgtttctcgt ctgcttcaaa accggctttt 1380

.......... ......... Orf frame 3 ......... .......... ..........

NarI(1389) .......... .......... .......... .......... ..........

1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt 1440

1381 tgtagttccg cggactatgc ttacgtaaac gttattgtta acgctataaa ttataacaaa 1440

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag 1500

1441 tacttcgaca aactgaagtt ttgtgtgttt ttttttttat tttgtttaat aaactttctc 1500

Orf frame 3 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta 1560

1501 ttaatcctta gcctgtcgaa tagcaatgcc cgattgtcgt gtggctctgc tttatcgaat 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga 1620

1561 ggactgcagt gtcggagacc ttcttgacgg cggttcgtct ctctctctct ttttctccct 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc 1680

1621 ctcgtcgaat ctggcgtaca cgaacacaca ctccgcagag agagaagcag aggacaaacg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... ClaI(1708) .......... .......... ..........

1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc 1740

1681 cgtttgcgta tctgacgtga ctcttttagc taatggataa aaaatactta cttataaacg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca 1800

1741 tgataatgat aagttttgat aattctatcg ttagtgtaag ttatcggttt atgatatggt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat 1860

1801 ggactcgcta cgttgcttta ctagttaaac tcgtttttac gacgtataaa tcctgccgta 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta 1920

1861 gtaatatctt tacgaagaac gacacatgaa aagagagcag accgtcgaca aagcggcaat 1920

.......... .......... .......... .......... .......... ..........

......... AflII(1934) ......... .......... .......... ..........

1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag 1980

1921 aacaattttg gccgaattca atccacacaa aagatgctga tcactacggg gatgatcttc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta 2040

1981 tacacacaac gtgtttacag ggacttattg gttaaacttc acgtctatcg tcatttgcat 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac 2100

2041 tcgattatac ttataataaa ttgacattac aaaattatag cgacctgtaa tgattatttg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa 2160

2101 ggtgatattt gtgtacatgt atacatacaa aaccgtatgt tactcatcaa cccctttttt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga 2220

2161 acacattttc gtggcactgg tagtgtcgta tttctattgg tcgacttcat agcttatact 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NcoI(2261) ..........

2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca 2280

2221 cattgggggt ttaacttagt gtacggcgtt gactatcctg ggtaccttca tgtgagaagt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa 2340

2281 accgctatat gttctgtgtg tgttcgtgct tgtgggtcaa cgcctccttt aagaggcatt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact 2400

2341 tacttttggg ttagccgctt gttaagtatg ggtatatacc attttcaaaa cttgcgctga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg 2460

2401 actctcgcct ctcgtaacgc cgactattcc aaaatcgcga ttcgcccgaa atattttgcc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct 2520

2461 cgacgccctg gtcaaaagta tagtgatggc aaactcaaga acacgacaca cctatgagga 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg 2580

2521 gggctgtgtt tcggcgaggt agtcggtcgt cagcagatta ggtctctggg gcctagcccc 2580

.......... AC5_primer .......... .......... .......... ..........

KpnI(2583) ........ EcoRI(2603) EcoRV(2615) NotI(2630) ........

2581 tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga 2640

2581 atggatgatc aggtcacacc accttaagac gtctataggt cgtgtcaccg ccggcgagct 2640

.......... .......... .......... .......... .......... ..........

XbaI(2642) ApaI(2652),BstBI(2655) ........ .......... ..........

2641 gtctagaggg cccttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac 2700

2641 cagatctccc gggaagcttc cattcggata gggattggga gaggagccag agctaagatg 2700

.......... .......... .......... V5 .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt 2760

2701 cgcatggcca gtagtagtgg tagtggtaac tcaaatttgg gcgactagtc ggagctgaca 2760

.......... 6xHis .......... ........ BGH_rev_primer ........

.... StuI(2771) SacI(2779) ... BamHI(2800) ......... ..........

2761 gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata 2820

2761 cggaagattc cggactcgag cgactagtcg gagctagctc ctaggtctgt actattctat 2820

.......... .......... .......... ........ EBV_rev_primer SV40_PA_terminator

.......... .......... .......... .......... .......... ..........

2821 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 2880

2821 gtaactactc aaacctgttt ggtgttgatc ttacgtcact ttttttacga aataaacact 2880

.... EBV_rev_primer SV40_PA_terminator .... .......... ..........

.......... .......... .......... .......... .......... HpaI(2935)

2881 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 2940

2881 ttaaacacta cgataacgaa ataaacattg gtaatattcg acgttatttg ttcaattgtt 2940

.......... ...... SV40_PA_terminator ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 3000

2941 gttgttaacg taagtaaaat acaaagtcca agtccccctc cacaccctcc aaaaaatttc 3000

.......... .......... .......... OFR3 .......... ..........

.......... .......... .......... .......... SalI(3043) ..........

3001 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga 3060

3001 gttcattttg gagatgttta caccataccg actaatacta gtcagctggc tacgggaact 3060

.......... .......... OFR3 .......... OFR3 ..........

.......... .......... .......... .......... .......... ..........

3061 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 3120

3061 ctcggaagtt gggtcagtcg aggaaggcca cccgcgcccc gtactgatag cagcggcgtg 3120

.......... ... OFR3 OFR3 tet(1032-576) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 3180

3121 aatactgaca gaagaaatag tacgttgagc atcctgtcca cggccgtcgc gagacccagt 3180

.......... ... OFR3 OFR3 tet(1032-576) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 3240

3181 aaaagccgct cctggcgaaa gcgacctcgc gctgctacta gccggacagc gaacgccata 3240

.......... ... OFR3 OFR3 tet(1032-576) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 3300

3241 agccttagaa cgtgcgggag cgagttcgga agcagtgacc agggcggtgg tttgcaaagc 3300

.......... ... OFR3 OFR3 tet(1032-576) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg 3360

3301 cgctcttcgt ccggtaatag cggccgtacc gccggctgcg cgacccgatg cagaacgacc 3360

.......... ... OFR3 OFR3 tet(1032-576) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca 3420

3361 gcaagcgctg cgctccgacc taccggaagg ggtaatacta agaagagcga aggccgccgt 3420

.......... ... OFR3 OFR3 tet(1032-576) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac 3480

3421 agccctacgg gcgcaacgtc cggtacgaca ggtccgtcca tctactgctg gtagtccctg 3480

.......... ... OFR3 OFR3 tet(1032-576) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agcttcaagg atcgctcgcg gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg 3540

3481 tcgaagttcc tagcgagcgc cgagaatggt cggtcgtttt ccggtccttg gcatttttcc 3540

........ tet(1032-576) OFR3 OFR3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3600

3541 ggcgcaacga ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg 3600

.......... ........ OFR3 OFR3 pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3660

3601 cgagttcagt ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac 3660

........ OFR3 pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3720

3661 cttcgaggga gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 3780

3721 aagagggaag cccttcgcac cgcgaaagag ttacgagtgc gacatccata gagtcaagcc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3840

3781 acatccagca agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 3900

3841 cgcggaatag gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3960

3901 accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 4020

3961 agaacttcac caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4080

4021 acgacttcgg tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4140

4081 ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4200

4141 gagttcttct aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg 4200

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4260

4201 caattcccta aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 4320

4261 tttttacttc aaaatttagt tagatttcat atatactcat ttgaaccaga ctgtcaatgg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4380

4321 ttacgaatta gtcactccgt ggatagagtc gctagacaga taaagcaagt aggtatcaac 4380

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4440

4381 ggactgaggg gcagcacatc tattgatgct atgccctccc gaatggtaga ccggggtcac 4440

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4500

4441 gacgttacta tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt tatttggtcg 4500

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4560

4501 gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa taggcggagg taggtcagat 4560

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4620

4561 aattaacaac ggcccttcga tctcattcat caagcggtca attatcaaac gcgttgcaac 4620

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 4680

4621 aacggtaacg acgtccgtag caccacagtg cgagcagcaa accataccga agtaagtcga 4680

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 4740

4681 ggccaagggt tgctagttcc gctcaatgta ctagggggta caacacgttt tttcgccaat 4740

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 4800

4741 cgaggaagcc aggaggctag caacagtctt cattcaaccg gcgtcacaat agtgagtacc 4800

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 4860

4801 aataccgtcg tgacgtatta agagaatgac agtacggtag gcattctacg aaaagacact 4860

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 4920

4861 gaccactcat gagttggttc agtaagactc ttatcacata cgccgctggc tcaacgagaa 4920

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4980

4921 cgggccgcag ttgtgcccta ttatggcgcg gtgtatcgtc ttgaaatttt cacgagtagt 4980

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 5040

4981 aaccttttgc aagaagcccc gcttttgaga gttcctagaa tggcgacaac tctaggtcaa 5040

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 5100

5041 gctacattgg gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag tggtcgcaaa 5100

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 5160

5101 gacccactcg tttttgtcct tccgttttac ggcgtttttt cccttattcc cgctgtgcct 5160

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 5220

5161 ttacaactta tgagtatgag aaggaaaaag ttataataac ttcgtaaata gtcccaataa 5220

Orf frame 2 Ampicillin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 5280

5221 cagagtactc gcctatgtat aaacttacat aaatcttttt atttgtttat ccccaaggcg 5280

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 5340

5281 cgtgtaaagg ggcttttcac ggtggactgc agattctttg gtaataatag tactgtaatt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cctataaaaa taggcgtatc acgaggccct ttcgt..... .......... .......... 5375

5341 ggatattttt atccgcatag tgctccggga aagca..... .......... .......... 5375

.......... .......... .......... .......... .......... ..........