Sequence of pAc5.1_V5-His

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Sequence of pAc5.1_V5-His_B

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Sequence of pAc5.1/V5-His B : 5379 BP; 1459 A; 1255 C; 1233 G; 1432 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga 60

1 gaagttcctt aattaagata taagattttt gtgtttacta tgaagatttt ttttagtact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac 120

61 taccgtagtt gagacttagt ttagaaacgt ctacgtggat gaagagtaaa ggtgacagtg 120

.......... .......... .......... .......... .......... ..........

.......... BglII(132) .......... .......... .......... ..........

121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg 180

121 tagtaaaaag gtctagagcg acggacaata caccgggtgt ttggttctgt gcaaaatacc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga 240

181 ggtaatttcg accgactagc agcggtttgt ggtttatgta tagttataca tgtaagctct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg 300

241 ttcttcgcta gtttcttcgc agaagcccgc tcatcctctt acgcctcctc ttcctcttgc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc 360

301 tcgactagat catagagagg tgttaggtta cggttgactg gttgaccggt ataagcctcg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac 420

361 ttaaacttcg gttaaaggta gcggaccgct agcgaggtaa gaaccgatat acaaaaagtg 420

.......... .......... .......... .......... .......... ..........

SmaI(427) .......... .......... .......... .......... ..........

421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct 480

421 gcaagggccc cggtaaaagt ttctgagcag ccattctatt ctaacacagt gagcgacaga 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag 540

481 gagaagtaaa cagcttctta cgactcctta aagcgctact gcagccgctc ataaaacttc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt 600

541 ttactcttat taaacataaa tatgctttta gtcaatcacc ttaaaagatg tttttgtaca 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac 660

601 atagatatct attaaaacaa cgttttatac aactgatact gtttctaaca tacatatatg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt 720

661 gaaattacat aagagtaaaa gaatacataa atattaccgt tactactatg actactataa 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg 780

721 aattctacta cggtctggtg tccgactaaa gacgcagaaa acggcttgcg tcacgtacac 780

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct 840

781 gccaacaaca aaaaacctta tcaaagttaa aagcctgaca ggcgaaacta aagtcaaaga 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca 900

841 accgaataag tttttcgttt catttcggtt ttttcgctct accgttatgg tttacgccgt 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg 960

901 tttgccatca ccttcctttc cccacgcccc gtcgccttcc ttcccacccc gccccgcacc 960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg 1020

961 gccccagaca ccgacccgcg ctgcagtggc tgcaacctcg gtgaggaaac tggtacacac 1020

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc 1080

1021 gcacacacat aataagcaca gagcggtgag cggccaacaa aaaaagaaaa atagagcgag 1080

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg 1140

1081 agagatcgcg gtagagcatg cgtacgagtt gcgtggcgta caacggcaca ggaaatacgc 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa 1200

1141 agtaaaaccg agctttatcc gttaataaat ttgtttctaa tcagttgctt ttgcgatttt 1200

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa 1260

1201 atttattcag atgttatacc aatgaataac ggtacacaca cgtcggttgc tatcgttgtt 1260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag 1320

1261 ttcgttgttg tgtcaccgaa agggagaaag tgaaaaacaa acgttcgcgc acgctcgttc 1320

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa 1380

1321 tgccgtgctg gccgtttgcg ttaatgcgac tgtttctcgt ctgcttcaaa accggctttt 1380

.......... .......... ORF 3 .......... .......... ..........

NarI(1389) .......... .......... .......... .......... ..........

1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt 1440

1381 tgtagttccg cggactatgc ttacgtaaac gttattgtta acgctataaa ttataacaaa 1440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag 1500

1441 tacttcgaca aactgaagtt ttgtgtgttt ttttttttat tttgtttaat aaactttctc 1500

ORF 3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta 1560

1501 ttaatcctta gcctgtcgaa tagcaatgcc cgattgtcgt gtggctctgc tttatcgaat 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga 1620

1561 ggactgcagt gtcggagacc ttcttgacgg cggttcgtct ctctctctct ttttctccct 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc 1680

1621 ctcgtcgaat ctggcgtaca cgaacacaca ctccgcagag agagaagcag aggacaaacg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... ClaI(1708) .......... .......... ..........

1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc 1740

1681 cgtttgcgta tctgacgtga ctcttttagc taatggataa aaaatactta cttataaacg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca 1800

1741 tgataatgat aagttttgat aattctatcg ttagtgtaag ttatcggttt atgatatggt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat 1860

1801 ggactcgcta cgttgcttta ctagttaaac tcgtttttac gacgtataaa tcctgccgta 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta 1920

1861 gtaatatctt tacgaagaac gacacatgaa aagagagcag accgtcgaca aagcggcaat 1920

.......... .......... .......... .......... .......... ..........

......... AflII(1934) ......... .......... .......... ..........

1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag 1980

1921 aacaattttg gccgaattca atccacacaa aagatgctga tcactacggg gatgatcttc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta 2040

1981 tacacacaac gtgtttacag ggacttattg gttaaacttc acgtctatcg tcatttgcat 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac 2100

2041 tcgattatac ttataataaa ttgacattac aaaattatag cgacctgtaa tgattatttg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa 2160

2101 ggtgatattt gtgtacatgt atacatacaa aaccgtatgt tactcatcaa cccctttttt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga 2220

2161 acacattttc gtggcactgg tagtgtcgta tttctattgg tcgacttcat agcttatact 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NcoI(2261) ..........

2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca 2280

2221 cattgggggt ttaacttagt gtacggcgtt gactatcctg ggtaccttca tgtgagaagt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa 2340

2281 accgctatat gttctgtgtg tgttcgtgct tgtgggtcaa cgcctccttt aagaggcatt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact 2400

2341 tacttttggg ttagccgctt gttaagtatg ggtatatacc attttcaaaa cttgcgctga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg 2460

2401 actctcgcct ctcgtaacgc cgactattcc aaaatcgcga ttcgcccgaa atattttgcc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct 2520

2461 cgacgccctg gtcaaaagta tagtgatggc aaactcaaga acacgacaca cctatgagga 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg 2580

2521 gggctgtgtt tcggcgaggt agtcggtcgt cagcagatta ggtctctggg gcctagcccc 2580

.......... AC5_pri .......... .......... .......... ..........

KpnI(2583) ........ EcoRI(2603) EcoRV(2615) NotI(2630) ........

2581 tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga 2640

2581 atggatgatc aggtcacacc accttaagac gtctataggt cgtgtcaccg ccggcgagct 2640

.......... .......... .......... .......... .......... ..........

XbaI(2642) ApaI(2652),SacII(2655),BstBI(2659) ...... ..........

2641 gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt 2700

2641 cagatctccc gggcgccaag cttccattcg gatagggatt gggagaggag ccagagctaa 2700

.......... .......... .......... .......... V5 ..........

.......... .......... .......... .......... .......... ..........

2701 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga 2760

2701 gatgcgcatg gccagtagta gtggtagtgg taactcaaat ttgggcgact agtcggagct 2760

.......... .......... 6xHis .......... .......... ..........

.......... StuI(2775) SacI(2783) ......... BamHI(2804) .........

2761 ctgtgccttc taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa 2820

2761 gacacggaag attccggact cgagcgacta gtcggagcta gctcctaggt ctgtactatt 2820

BGH_rev_pri ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 2880

2821 ctatgtaact actcaaacct gtttggtgtt gatcttacgt cacttttttt acgaaataaa 2880

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 2940

2881 cactttaaac actacgataa cgaaataaac attggtaata ttcgacgtta tttgttcaat 2940

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 3000

2941 tgttgttgtt aacgtaagta aaatacaaag tccaagtccc cctccacacc ctccaaaaaa 3000

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... SalI(3047) ..........

3001 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc 3060

3001 tttcgttcat tttggagatg tttacaccat accgactaat actagtcagc tggctacggg 3060

.......... .......... ORF 1 .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

3061 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 3120

3061 aactctcgga agttgggtca gtcgaggaag gccacccgcg ccccgtactg atagcagcgg 3120

.......... .. ORF 1 ORF 1 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg 3180

3121 cgtgaatact gacagaagaa atagtacgtt gagcatcctg tccacggccg tcgcgagacc 3180

.......... .. ORF 1 ORF 1 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg 3240

3181 cagtaaaagc cgctcctggc gaaagcgacc tcgcgctgct actagccgga cagcgaacgc 3240

.......... .. ORF 1 ORF 1 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt 3300

3241 cataagcctt agaacgtgcg ggagcgagtt cggaagcagt gaccagggcg gtggtttgca 3300

.......... .. ORF 1 ORF 1 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg 3360

3301 aagccgctct tcgtccggta atagcggccg taccgccggc tgcgcgaccc gatgcagaac 3360

.......... .. ORF 1 ORF 1 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc 3420

3361 gaccgcaagc gctgcgctcc gacctaccgg aaggggtaat actaagaaga gcgaaggccg 3420

.......... .. ORF 1 ORF 1 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag 3480

3421 ccgtagccct acgggcgcaa cgtccggtac gacaggtccg tccatctact gctggtagtc 3480

.......... .. ORF 1 ORF 1 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggacagcttc aaggatcgct cgcggctctt accagccagc aaaaggccag gaaccgtaaa 3540

3481 cctgtcgaag ttcctagcga gcgccgagaa tggtcggtcg ttttccggtc cttggcattt 3540

....... tet(1032-576) ORF 1 ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 3600

3541 ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg gactgctcgt agtgttttta 3600

.......... ....... ORF 1 ORF 1 pBR322_origin ....... ..........

.......... .......... .......... .......... .......... ..........

3601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 3660

3601 gctgcgagtt cagtctccac cgctttgggc tgtcctgata tttctatggt ccgcaaaggg 3660

.......... ..... ORF 1 pBR322_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 3720

3661 ggaccttcga gggagcacgc gagaggacaa ggctgggacg gcgaatggcc tatggacagg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 3780

3721 cggaaagagg gaagcccttc gcaccgcgaa agagttacga gtgcgacatc catagagtca 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 3840

3781 agccacatcc agcaagcgag gttcgacccg acacacgtgc ttggggggca agtcgggctg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 3900

3841 gcgacgcgga ataggccatt gatagcagaa ctcaggttgg gccattctgt gctgaatagc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 3960

3901 ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct ccatacatcc gccacgatgt 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 4020

3961 ctcaagaact tcaccaccgg attgatgccg atgtgatctt cctgtcataa accatagacg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 4080

4021 cgagacgact tcggtcaatg gaagcctttt tctcaaccat cgagaactag gccgtttgtt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 4140

4081 tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg tctaatgcgc gtcttttttt 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 4200

4141 cctagagttc ttctaggaaa ctagaaaaga tgccccagac tgcgagtcac cttgcttttg 4200

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 4260

4201 agtgcaattc cctaaaacca gtactctaat agtttttcct agaagtggat ctaggaaaat 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 4320

4261 ttaattttta cttcaaaatt tagttagatt tcatatatac tcatttgaac cagactgtca 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 4380

4321 atggttacga attagtcact ccgtggatag agtcgctaga cagataaagc aagtaggtat 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 4440

4381 caacggactg aggggcagca catctattga tgctatgccc tcccgaatgg tagaccgggg 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 4500

4441 tcacgacgtt actatggcgc tctgggtgcg agtggccgag gtctaaatag tcgttatttg 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 4560

4501 gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt gaaataggcg gaggtaggtc 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 4620

4561 agataattaa caacggccct tcgatctcat tcatcaagcg gtcaattatc aaacgcgttg 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 4680

4621 caacaacggt aacgacgtcc gtagcaccac agtgcgagca gcaaaccata ccgaagtaag 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 4740

4681 tcgaggccaa gggttgctag ttccgctcaa tgtactaggg ggtacaacac gttttttcgc 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 4800

4741 caatcgagga agccaggagg ctagcaacag tcttcattca accggcgtca caatagtgag 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 4860

4801 taccaatacc gtcgtgacgt attaagagaa tgacagtacg gtaggcattc tacgaaaaga 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 4920

4861 cactgaccac tcatgagttg gttcagtaag actcttatca catacgccgc tggctcaacg 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 4980

4921 agaacgggcc gcagttgtgc cctattatgg cgcggtgtat cgtcttgaaa ttttcacgag 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 5040

4981 tagtaacctt ttgcaagaag ccccgctttt gagagttcct agaatggcga caactctagg 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 5100

5041 tcaagctaca ttgggtgagc acgtgggttg actagaagtc gtagaaaatg aaagtggtcg 5100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 5160

5101 caaagaccca ctcgtttttg tccttccgtt ttacggcgtt ttttccctta ttcccgctgt 5160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 5220

5161 gcctttacaa cttatgagta tgagaaggaa aaagttataa taacttcgta aatagtccca 5220

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 5280

5221 ataacagagt actcgcctat gtataaactt acataaatct ttttatttgt ttatccccaa 5280

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 5340

5281 ggcgcgtgta aaggggcttt tcacggtgga ctgcagattc tttggtaata atagtactgt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttaacctata aaaataggcg tatcacgagg ccctttcgt. .......... .......... 5379

5341 aattggatat ttttatccgc atagtgctcc gggaaagca. .......... .......... 5379

.......... .......... .......... .......... .......... ..........