Sequence of pAc5.1_V5-His

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Sequence of pAc5.1_V5-His_C

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Sequence of pAc5.1/V5-His C : 5371 BP; 1459 A; 1254 C; 1227 G; 1431 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga 60

1 gaagttcctt aattaagata taagattttt gtgtttacta tgaagatttt ttttagtact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac 120

61 taccgtagtt gagacttagt ttagaaacgt ctacgtggat gaagagtaaa ggtgacagtg 120

.......... .......... .......... .......... .......... ..........

.......... BglII(132) .......... .......... .......... ..........

121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg 180

121 tagtaaaaag gtctagagcg acggacaata caccgggtgt ttggttctgt gcaaaatacc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga 240

181 ggtaatttcg accgactagc agcggtttgt ggtttatgta tagttataca tgtaagctct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg 300

241 ttcttcgcta gtttcttcgc agaagcccgc tcatcctctt acgcctcctc ttcctcttgc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc 360

301 tcgactagat catagagagg tgttaggtta cggttgactg gttgaccggt ataagcctcg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac 420

361 ttaaacttcg gttaaaggta gcggaccgct agcgaggtaa gaaccgatat acaaaaagtg 420

.......... .......... .......... .......... .......... ..........

SmaI(427) .......... .......... .......... .......... ..........

421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct 480

421 gcaagggccc cggtaaaagt ttctgagcag ccattctatt ctaacacagt gagcgacaga 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag 540

481 gagaagtaaa cagcttctta cgactcctta aagcgctact gcagccgctc ataaaacttc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt 600

541 ttactcttat taaacataaa tatgctttta gtcaatcacc ttaaaagatg tttttgtaca 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac 660

601 atagatatct attaaaacaa cgttttatac aactgatact gtttctaaca tacatatatg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt 720

661 gaaattacat aagagtaaaa gaatacataa atattaccgt tactactatg actactataa 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg 780

721 aattctacta cggtctggtg tccgactaaa gacgcagaaa acggcttgcg tcacgtacac 780

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct 840

781 gccaacaaca aaaaacctta tcaaagttaa aagcctgaca ggcgaaacta aagtcaaaga 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca 900

841 accgaataag tttttcgttt catttcggtt ttttcgctct accgttatgg tttacgccgt 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg 960

901 tttgccatca ccttcctttc cccacgcccc gtcgccttcc ttcccacccc gccccgcacc 960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg 1020

961 gccccagaca ccgacccgcg ctgcagtggc tgcaacctcg gtgaggaaac tggtacacac 1020

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc 1080

1021 gcacacacat aataagcaca gagcggtgag cggccaacaa aaaaagaaaa atagagcgag 1080

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg 1140

1081 agagatcgcg gtagagcatg cgtacgagtt gcgtggcgta caacggcaca ggaaatacgc 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa 1200

1141 agtaaaaccg agctttatcc gttaataaat ttgtttctaa tcagttgctt ttgcgatttt 1200

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa 1260

1201 atttattcag atgttatacc aatgaataac ggtacacaca cgtcggttgc tatcgttgtt 1260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag 1320

1261 ttcgttgttg tgtcaccgaa agggagaaag tgaaaaacaa acgttcgcgc acgctcgttc 1320

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa 1380

1321 tgccgtgctg gccgtttgcg ttaatgcgac tgtttctcgt ctgcttcaaa accggctttt 1380

.......... .......... ORF 3 .......... .......... ..........

NarI(1389) .......... .......... .......... .......... ..........

1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt 1440

1381 tgtagttccg cggactatgc ttacgtaaac gttattgtta acgctataaa ttataacaaa 1440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag 1500

1441 tacttcgaca aactgaagtt ttgtgtgttt ttttttttat tttgtttaat aaactttctc 1500

ORF 3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta 1560

1501 ttaatcctta gcctgtcgaa tagcaatgcc cgattgtcgt gtggctctgc tttatcgaat 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga 1620

1561 ggactgcagt gtcggagacc ttcttgacgg cggttcgtct ctctctctct ttttctccct 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc 1680

1621 ctcgtcgaat ctggcgtaca cgaacacaca ctccgcagag agagaagcag aggacaaacg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... ClaI(1708) .......... .......... ..........

1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc 1740

1681 cgtttgcgta tctgacgtga ctcttttagc taatggataa aaaatactta cttataaacg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca 1800

1741 tgataatgat aagttttgat aattctatcg ttagtgtaag ttatcggttt atgatatggt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat 1860

1801 ggactcgcta cgttgcttta ctagttaaac tcgtttttac gacgtataaa tcctgccgta 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta 1920

1861 gtaatatctt tacgaagaac gacacatgaa aagagagcag accgtcgaca aagcggcaat 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag 1980

1921 aacaattttg gccgaattca atccacacaa aagatgctga tcactacggg gatgatcttc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta 2040

1981 tacacacaac gtgtttacag ggacttattg gttaaacttc acgtctatcg tcatttgcat 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac 2100

2041 tcgattatac ttataataaa ttgacattac aaaattatag cgacctgtaa tgattatttg 2100

.......... .......... .......... .......... .......... ..........

.......... NdeI(2120) .......... .......... .......... ..........

2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa 2160

2101 ggtgatattt gtgtacatgt atacatacaa aaccgtatgt tactcatcaa cccctttttt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga 2220

2161 acacattttc gtggcactgg tagtgtcgta tttctattgg tcgacttcat agcttatact 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NcoI(2261) ..........

2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca 2280

2221 cattgggggt ttaacttagt gtacggcgtt gactatcctg ggtaccttca tgtgagaagt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa 2340

2281 accgctatat gttctgtgtg tgttcgtgct tgtgggtcaa cgcctccttt aagaggcatt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact 2400

2341 tacttttggg ttagccgctt gttaagtatg ggtatatacc attttcaaaa cttgcgctga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg 2460

2401 actctcgcct ctcgtaacgc cgactattcc aaaatcgcga ttcgcccgaa atattttgcc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct 2520

2461 cgacgccctg gtcaaaagta tagtgatggc aaactcaaga acacgacaca cctatgagga 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg 2580

2521 gggctgtgtt tcggcgaggt agtcggtcgt cagcagatta ggtctctggg gcctagcccc 2580

.......... AC5_pri .......... .......... .......... ..........

KpnI(2583) ........ EcoRI(2603) EcoRV(2615) NotI(2630) ........

2581 tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga 2640

2581 atggatgatc aggtcacacc accttaagac gtctataggt cgtgtcaccg ccggcgagct 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggtcacccat tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt 2700

2641 ccagtgggta agcttccatt cggataggga ttgggagagg agccagagct aagatgcgca 2700

.......... .......... .......... V5 .......... ..........

.......... .......... .......... .......... .......... ..........

2701 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct 2760

2701 tggccagtag tagtggtagt ggtaactcaa atttgggcga ctagtcggag ctgacacgga 2760

.......... 6xHis .......... .......... ......... BGH_rev_pri

StuI(2767) SacI(2775) ......... BamHI(2796) ......... ..........

2761 tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat aagatacatt 2820

2761 agattccgga ctcgagcgac tagtcggagc tagctcctag gtctgtacta ttctatgtaa 2820

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2821 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 2880

2821 ctactcaaac ctgtttggtg ttgatcttac gtcacttttt ttacgaaata aacactttaa 2880

EBV_rev_pri ....... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 2940

2881 acactacgat aacgaaataa acattggtaa tattcgacgt tatttgttca attgttgttg 2940

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag 3000

2941 ttaacgtaag taaaatacaa agtccaagtc cccctccaca ccctccaaaa aatttcgttc 3000

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... SalI(3039) .......... ..........

3001 taaaacctct acaaatgtgg tatggctgat tatgatcagt cgaccgatgc ccttgagagc 3060

3001 attttggaga tgtttacacc ataccgacta atactagtca gctggctacg ggaactctcg 3060

.......... .......... ORF 2 ........ ORF 2 tet(1032-576)

.......... .......... .......... .......... .......... ..........

3061 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 3120

3061 gaagttgggt cagtcgagga aggccacccg cgccccgtac tgatagcagc ggcgtgaata 3120

.......... .. ORF 2 ORF 2 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt 3180

3121 ctgacagaag aaatagtacg ttgagcatcc tgtccacggc cgtcgcgaga cccagtaaaa 3180

.......... .. ORF 2 ORF 2 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg 3240

3181 gccgctcctg gcgaaagcga cctcgcgctg ctactagccg gacagcgaac gccataagcc 3240

.......... .. ORF 2 ORF 2 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga 3300

3241 ttagaacgtg cgggagcgag ttcggaagca gtgaccaggg cggtggtttg caaagccgct 3300

.......... .. ORF 2 ORF 2 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt 3360

3301 cttcgtccgg taatagcggc cgtaccgccg gctgcgcgac ccgatgcaga acgaccgcaa 3360

.......... .. ORF 2 ORF 2 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg 3420

3361 gcgctgcgct ccgacctacc ggaaggggta atactaagaa gagcgaaggc cgccgtagcc 3420

.......... .. ORF 2 ORF 2 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct 3480

3421 ctacgggcgc aacgtccggt acgacaggtc cgtccatcta ctgctggtag tccctgtcga 3480

.......... .. ORF 2 ORF 2 tet(1032-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcaaggatcg ctcgcggctc ttaccagcca gcaaaaggcc aggaaccgta aaaaggccgc 3540

3481 agttcctagc gagcgccgag aatggtcggt cgttttccgg tccttggcat ttttccggcg 3540

....... tet(1032-576) ORF 2 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 3600

3541 caacgaccgc aaaaaggtat ccgaggcggg gggactgctc gtagtgtttt tagctgcgag 3600

.......... .. ORF 2 ORF 2 pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 3660

3601 ttcagtctcc accgctttgg gctgtcctga tatttctatg gtccgcaaag ggggaccttc 3660

........ ORF 2 pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 3720

3661 gagggagcac gcgagaggac aaggctggga cggcgaatgg cctatggaca ggcggaaaga 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta 3780

3721 gggaagccct tcgcaccgcg aaagagttac gagtgcgaca tccatagagt caagccacat 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 3840

3781 ccagcaagcg aggttcgacc cgacacacgt gcttgggggg caagtcgggc tggcgacgcg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 3900

3841 gaataggcca ttgatagcag aactcaggtt gggccattct gtgctgaata gcggtgaccg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 3960

3901 tcgtcggtga ccattgtcct aatcgtctcg ctccatacat ccgccacgat gtctcaagaa 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 4020

3961 cttcaccacc ggattgatgc cgatgtgatc ttcctgtcat aaaccataga cgcgagacga 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4080

4021 cttcggtcaa tggaagcctt tttctcaacc atcgagaact aggccgtttg tttggtggcg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 4140

4081 accatcgcca ccaaaaaaac aaacgttcgt cgtctaatgc gcgtcttttt ttcctagagt 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 4200

4141 tcttctagga aactagaaaa gatgccccag actgcgagtc accttgcttt tgagtgcaat 4200

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 4260

4201 tccctaaaac cagtactcta atagtttttc ctagaagtgg atctaggaaa atttaatttt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 4320

4261 tacttcaaaa tttagttaga tttcatatat actcatttga accagactgt caatggttac 4320

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

4321 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 4380

4321 gaattagtca ctccgtggat agagtcgcta gacagataaa gcaagtaggt atcaacggac 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 4440

4381 tgaggggcag cacatctatt gatgctatgc cctcccgaat ggtagaccgg ggtcacgacg 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4500

4441 ttactatggc gctctgggtg cgagtggccg aggtctaaat agtcgttatt tggtcggtcg 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4560

4501 gccttcccgg ctcgcgtctt caccaggacg ttgaaatagg cggaggtagg tcagataatt 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 4620

4561 aacaacggcc cttcgatctc attcatcaag cggtcaatta tcaaacgcgt tgcaacaacg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4680

4621 gtaacgacgt ccgtagcacc acagtgcgag cagcaaacca taccgaagta agtcgaggcc 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 4740

4681 aagggttgct agttccgctc aatgtactag ggggtacaac acgttttttc gccaatcgag 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 4800

4741 gaagccagga ggctagcaac agtcttcatt caaccggcgt cacaatagtg agtaccaata 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4860

4801 ccgtcgtgac gtattaagag aatgacagta cggtaggcat tctacgaaaa gacactgacc 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 4920

4861 actcatgagt tggttcagta agactcttat cacatacgcc gctggctcaa cgagaacggg 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 4980

4921 ccgcagttgt gccctattat ggcgcggtgt atcgtcttga aattttcacg agtagtaacc 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 5040

4981 ttttgcaaga agccccgctt ttgagagttc ctagaatggc gacaactcta ggtcaagcta 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 5100

5041 cattgggtga gcacgtgggt tgactagaag tcgtagaaaa tgaaagtggt cgcaaagacc 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 5160

5101 cactcgtttt tgtccttccg ttttacggcg ttttttccct tattcccgct gtgcctttac 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 5220

5161 aacttatgag tatgagaagg aaaaagttat aataacttcg taaatagtcc caataacaga 5220

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 5280

5221 gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc aaggcgcgtg 5280

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 5340

5281 taaaggggct tttcacggtg gactgcagat tctttggtaa taatagtact gtaattggat 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 taaaaatagg cgtatcacga ggccctttcg t......... .......... .......... 5371

5341 atttttatcc gcatagtgct ccgggaaagc a......... .......... .......... 5371

.......... .......... .......... .......... .......... ..........