Sequence of pAcGFP1-N1

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Sequence of pAcGFP1-N1

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Sequence of pAcGFP1-N1 : 4726 BP; 1138 A; 1268 C; 1240 G; 1080 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

BglII,XhoI SacI,HindIII EcoRI PstI(638),SalI(639)

601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 660

601 ggcctgagtc tagagctcga gttcgaagct taagacgtca gctgccatgg cgcccgggcc 660

.......... .......... .......... .......... .......... ..........

AgeI(666) .......... .......... .......... .......... ..........

661 gatccaccgg tcatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg 720

661 ctaggtggcc agtaccactc gttcccgcgg ctcgacaagt ggccgtagca cgggtaggac 720

.......... .......... ...... EGFP(0-717) ORF 1 ...... ..........

.......... .......... .......... .......... .......... ..........

721 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc 780

721 tagctcgact taccgctaca cttaccggtg ttcaagtcgc actcgccgct cccgctcccg 780

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg 840

781 ctacggtgga tgccgttcga ctgggacttc aagtagacgt ggtggccgtt cgacggacac 840

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc 900

841 gggaccgggt gggaccactg gtgggactcg atgccgcacg tcacgaagag tgcgatgggg 900

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag 960

901 ctagtgtact tcgtcgtgct gaagaagttc tcgcggtacg gactcccgat gtaggtcctc 960

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag 1020

961 gcgtggtaga agaagctcct actgccgttg atgttcagcg cgcggctcca cttcaagctc 1020

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac 1080

1021 ccgctatggg accacttagc gtagctcgac tggccgtggc taaagttcct cctaccgttg 1080

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac 1140

1081 taggacccgt tattctacct catgttgatg ttgcgggtgt tacacatgta gtactggctg 1140

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc 1200

1141 ttccggttct taccgtagtt ccacttgaag ttctaggcgg tgttgtagct cctaccgtcg 1200

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg 1260

1201 cacgtcgacc ggctggtgat ggtcgtctta tgggggtagc cgctaccggg acacgacgac 1260

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc 1320

1261 gggctattgg tgatggacag gtgggtctcg cgggacaggt tcctggggtt gctcttcgcg 1320

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag 1380

1321 ctagtgtact agatgaagcc gaagcactgg cggcggcggt agtgggtgcc gtacctactc 1380

.......... ...... EGFP(0-717) ORF 1 ...... .......... ..........

.......... NotI(1394) .......... .......... .......... ..........

1381 ctgtacaagt gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt 1440

1381 gacatgttca ctcgccggcg ctgagatcta gtattagtcg gtatggtgta aacatctcca 1440

EGFP(0-717) ORF 1 .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 1500

1441 aaatgaacga aattttttgg agggtgtgga gggggacttg gactttgtat tttacttacg 1500

.......... .......... .......... .......... .......... ..........

.......... HpaI(1513) .......... .......... .......... ..........

1501 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 1560

1501 ttaacaacaa caattgaaca aataacgtcg aatattacca atgtttattt cgttatcgta 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 1620

1561 gtgtttaaag tgtttatttc gtaaaaaaag tgacgtaaga tcaacaccaa acaggtttga 1620

.......... .......... .......... .......... ......... EBV_rev_pri

......... AflII(1632) ......... .......... .......... ..........

1621 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 1680

1621 gtagttacat agaattccgc atttaacatt cgcaattata aaacaatttt aagcgcaatt 1680

EBV_rev_pri ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 1740

1681 taaaaacaat ttagtcgagt aaaaaattgg ttatccggct ttagccgttt tagggaatat 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 1800

1741 ttagttttct tatctggctc tatcccaact cacaacaagg tcaaaccttg ttctcaggtg 1800

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

1801 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 1860

1801 ataatttctt gcacctgagg ttgcagtttc ccgctttttg gcagatagtc ccgctaccgg 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 1920

1861 gtgatgcact tggtagtggg attagttcaa aaaaccccag ctccacggca tttcgtgatt 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 1980

1921 tagccttggg atttccctcg ggggctaaat ctcgaactgc ccctttcggc cgcttgcacc 1980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 2040

1981 gctctttcct tcccttcttt cgctttcctc gcccgcgatc ccgcgaccgt tcacatcgcc 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 2100

2041 agtgcgacgc gcattggtgg tgtgggcggc gcgaattacg cggcgatgtc ccgcgcagtc 2100

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 2160

2101 caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 2220

2161 gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt 2220

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 2280

2221 ccttctcagg actccgcctt tcttggtcga caccttacac acagtcaatc ccacaccttt 2280

.......... .......... .......... ..... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

2281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 2340

2281 caggggtccg aggggtcgtc cgtcttcata cgtttcgtac gtagagttaa tcagtcgttg 2340

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 2400

2341 gtccacacct ttcaggggtc cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt 2400

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa c tccgcccag 2460

2401 aatcagtcgt tggtatcagg gcggggattg aggcgggtag ggcggggatt gaggcgggtc 2460

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

2461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 2520

2461 aaggcgggta agaggcgggg taccgactga ttaaaaaaaa taaatacgtc tccggctccg 2520

SV40_enh ..... SV40_pro SV40_origin ... SV40pro_F_pri ........

.......... .......... .......... .......... .......... ..........

2521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 2580

2521 gcggagccgg agactcgata aggtcttcat cactcctccg aaaaaacctc cggatccgaa 2580

SV40_pro .......... .......... .......... .......... ..........

ClaI(2590) .......... .......... .......... .......... ..........

2581 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 2640

2581 aacgtttcta gctagttctc tgtcctactc ctagcaaagc gtactaactt gttctaccta 2640

.......... .......... .......... .......... ........ ORF 3 Neomycin

.......... .......... .......... .......... .......... ..........

2641 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 2700

2641 acgtgcgtcc aagaggccgg cgaacccacc tctccgataa gccgatactg acccgtgttg 2700

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 2760

2701 tctgttagcc gacgagacta cggcggcaca aggccgacag tcgcgtcccc gcgggccaag 2760

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 2820

2761 aaaaacagtt ctggctggac aggccacggg acttacttga cgttctgctc cgtcgcgccg 2820

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... FspI(2852) .......... ..........

2821 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 2880

2821 atagcaccga ccggtgctgc ccgcaaggaa cgcgtcgaca cgagctgcaa cagtgacttc 2880

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 2940

2881 gcccttccct gaccgacgat aacccgcttc acggccccgt cctagaggac agtagagtgg 2940

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 3000

2941 aacgaggacg gctctttcat aggtagtacc gactacgtta cgccgccgac gtatgcgaac 3000

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 3060

3001 taggccgatg gacgggtaag ctggtggttc gctttgtagc gtagctcgct cgtgcatgag 3060

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 3120

3061 cctaccttcg gccagaacag ctagtcctac tagacctgct tctcgtagtc cccgagcgcg 3120

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 3180

3121 gtcggcttga caagcggtcc gagttccgct cgtacgggct gccgctccta gagcagcact 3180

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 3240

3181 gggtaccgct acggacgaac ggcttatagt accacctttt accggcgaaa agacctaagt 3240

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 3300

3241 agctgacacc ggccgaccca caccgcctgg cgatagtcct gtatcgcaac cgatgggcac 3300

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 3360

3301 tataacgact tctcgaaccg ccgcttaccc gactggcgaa ggagcacgaa atgccatagc 3360

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 3420

3361 ggcgagggct aagcgtcgcg tagcggaaga tagcggaaga actgctcaag aagactcgcc 3420

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 3480

3421 ctgagacccc aagctttact ggctggttcg ctgcgggttg gacggtagtg ctctaaagct 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 3540

3481 aaggtggcgg cggaagatac tttccaaccc gaagccttag caaaaggccc tgcggccgac 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 3600

3541 ctactaggag gtcgcgcccc tagagtacga cctcaagaag cgggtgggat ccccctccga 3600

.......... .......... .......... .......... .......... TK_PA_term

.......... .......... .......... .......... .......... ..........

3601 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 3660

3601 ttgactttgt gccttcctct gttatggcct tccttgggcg cgatactgcc gttatttttc 3660

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 3720

3661 tgtcttattt tgcgtgccac aacccagcaa acaagtattt gcgccccaag ccagggtccc 3720

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 3780

3721 gaccgtgaga cagctatggg gtggctctgg ggtaaccccg gttatgcggg cgcaaagaag 3780

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 3840

3781 gaaaaggggt ggggtggggg gttcaagccc acttccgggt cccgagcgtc ggttgcagcc 3840

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 3900

3841 ccgccgtccg ggacggtatc ggagtccaat gagtatatat gaaatctaac taaattttga 3900

TK_PA_term .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 3960

3901 agtaaaaatt aaattttcct agatccactt ctaggaaaaa ctattagagt actggtttta 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4020

3961 gggaattgca ctcaaaagca aggtgactcg cagtctgggg catcttttct agtttcctag 4020

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4080

4021 aagaactcta ggaaaaaaag acgcgcatta gacgacgaac gtttgttttt ttggtggcga 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4140

4081 tggtcgccac caaacaaacg gcctagttct cgatggttga gaaaaaggct tccattgacc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 4200

4141 gaagtcgtct cgcgtctatg gtttatgaca ggaagatcac atcggcatca atccggtggt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4260

4201 gaagttcttg agacatcgtg gcggatgtat ggagcgagac gattaggaca atggtcaccg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4320

4261 acgacggtca ccgctattca gcacagaatg gcccaacctg agttctgcta tcaatggcct 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... ......... ApaLI(4354) ......... ..........

4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4380

4321 attccgcgtc gccagcccga cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4440

4381 ctggatgtgg cttgactcta tggatgtcgc actcgatact ctttcgcggt gcgaagggct 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4500

4441 tccctctttc cgcctgtcca taggccattc gccgtcccag ccttgtcctc tcgcgtgctc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4560

4501 cctcgaaggt ccccctttgc ggaccataga aatatcagga cagcccaaag cggtggagac 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4620

4561 tgaactcgca gctaaaaaca ctacgagcag tccccccgcc tcggatacct ttttgcggtc 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 4680

4621 gttgcgccgg aaaaatgcca aggaccggaa aacgaccgga aaacgagtgt acaagaaagg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat.... .......... 4726

4681 acgcaatagg ggactaagac acctattggc ataatggcgg tacgta.... .......... 4726

.......... .......... .......... .......... .......... ..........