(_(_LOCUS pAcGFP1 3354 bp DNA SYN 06-Sep-2007 DEFINITION pAcGFP1 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3354 /organism="pAcGFP1" /mol_type="other DNA" promoter 143..172 /label="lac_promoter" misc_feature 186..208 /label="M13_pUC_rev_primer" misc_feature 207..225 /label="M13_reverse_primer" CDS 217..1008 /label="EGFP(0-717)" /gene="EGFP(0-717)" /note="ORF frame 1" /translation="MTMITPSLHACRSTLEDPRVPVATMVSKGAELFTGIVPILIELN GDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDH MKQHDFFKSAMPEGYIQERTIFFEDDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGN ILGNKMEYNYNAHNVYIMTDKAKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPV LLPDNHYLSTQSALSKDPNEKRDHMIYFGFVTAAAITHGMDELYK*" gene 289..1005 /label="EGFP(0-717)" /gene="EGFP(0-717)" misc_feature complement(1144..1166) /label="pGEX_3_primer" promoter 1484..1512 /label="AmpR_promoter" CDS 1554..2414 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 1554..2414 /label="Ampicillin" /gene="Ampicillin" rep_origin 2569..3188 /label="pBR322_origin" ORIGIN 1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTTG 241 CATGCCTGCA GGTCGACTCT AGAGGATCCC CGGGTACCGG TCGCCACCAT GGTGAGCAAG 301 GGCGCCGAGC TGTTCACCGG CATCGTGCCC ATCCTGATCG AGCTGAATGG CGATGTGAAT 361 GGCCACAAGT TCAGCGTGAG CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 421 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCTGTGCCCT GGCCCACCCT GGTGACCACC 481 CTGAGCTACG GCGTGCAGTG CTTCTCACGC TACCCCGATC ACATGAAGCA GCACGACTTC 541 TTCAAGAGCG CCATGCCTGA GGGCTACATC CAGGAGCGCA CCATCTTCTT CGAGGATGAC 601 GGCAACTACA AGTCGCGCGC CGAGGTGAAG TTCGAGGGCG ATACCCTGGT GAATCGCATC 661 GAGCTGACCG GCACCGATTT CAAGGAGGAT GGCAACATCC TGGGCAATAA GATGGAGTAC 721 AACTACAACG CCCACAATGT GTACATCATG ACCGACAAGG CCAAGAATGG CATCAAGGTG 781 AACTTCAAGA TCCGCCACAA CATCGAGGAT GGCAGCGTGC AGCTGGCCGA CCACTACCAG 841 CAGAATACCC CCATCGGCGA TGGCCCTGTG CTGCTGCCCG ATAACCACTA CCTGTCCACC 901 CAGAGCGCCC TGTCCAAGGA CCCCAACGAG AAGCGCGATC ACATGATCTA CTTCGGCTTC 961 GTGACCGCCG CCGCCATCAC CCACGGCATG GATGAGCTGT ACAAGTGAGC GGCCGCGACT 1021 CTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 1081 TAGGCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 1141 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 1201 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 1261 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 1321 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT 1381 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 1441 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 1501 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 1561 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 1621 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 1681 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 1741 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 1801 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 1861 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 1921 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 1981 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 2041 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 2101 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 2161 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 2221 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 2281 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 2341 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 2401 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 2461 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 2521 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 2581 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 2641 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 2701 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC 2761 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 2821 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 2881 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 2941 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG 3001 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 3061 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 3121 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 3181 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 3241 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 3301 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAG // pAcGFP1 "Times New Roman(_C lac_pro"Times New Roman(_C M13_pUC_rev_pri"Times New Roman(_CɆ M13_reverse_pri"Times New Roman(_ ORF 1"Times New Roman((_C ! EGFP(0-717)"Times New Roman(_C x pGEX_3_pri"Times New Roman(_CɆ AmpR_pro"Times New Roman(_ n ORF 3"Times New Roman(_C n  Ampicillin"Times New Roman(_C t  pBR322_origin"Times New Roman (_HindIII"Times New Roman CqK_;D5;D (_PstI(250), SalI(252)"Times New RomanT ItRbREC aE (_+BamHI(264), SmaI(271), AgeI(276), KpnI(277)"Times New Roman Ry\hK-i (_NcoI(287), NarI(302), MscI(362)"Times New Roman.  X}oti (_;NotI(1010), EcoRI(1024), StuI(1084), SpeI(1088), ApaI(1107)"Times New Roman< p  (_NdeI"Times New Roman  lx (_AflII"Times New RomanL [_dd (_AatII"Times New Roman  CWii  (_FspI"Times New RomanF  TD44 (_test"Times New Roman d:){)d