Sequence of pAcGFP1

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Sequence of pAcGFP1

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Sequence of pAcGFP1 : 3354 BP; 828 A; 873 C; 883 G; 770 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... ......... HindIII(234)

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gc caagcttg 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggttcgaac 240

....... M13_pUC_rev_pri .... M13_reverse_pri ORF 1 .......

PstI,SalI BamHI264) SmaI(271),AgeI(276),KpnI(277)

241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcaag 300

241 gtacggacgt ccagctgaga tctcctaggg gcccatggcc agcggtggta ccactcgttc 300

.......... .......... ORF 1 .......... ......... EGFP(0-717)

.......... .......... .......... .......... .......... ..........

301 ggcgccgagc tgttcaccgg catcgtgccc atcctgatcg agctgaatgg cgatgtgaat 360

301 ccgcggctcg acaagtggcc gtagcacggg taggactagc tcgacttacc gctacactta 360

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

MscI(362) .......... .......... .......... .......... ..........

361 ggccacaagt tcagcgtgag cggcgagggc gagggcgatg ccacctacgg caagctgacc 420

361 ccggtgttca agtcgcactc gccgctcccg ctcccgctac ggtggatgcc gttcgactgg 420

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctgaagttca tctgcaccac cggcaagctg cctgtgccct ggcccaccct ggtgaccacc 480

421 gacttcaagt agacgtggtg gccgttcgac ggacacggga ccgggtggga ccactggtgg 480

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctgagctacg gcgtgcagtg cttctcacgc taccccgatc acatgaagca gcacgacttc 540

481 gactcgatgc cgcacgtcac gaagagtgcg atggggctag tgtacttcgt cgtgctgaag 540

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttcaagagcg ccatgcctga gggctacatc caggagcgca ccatcttctt cgaggatgac 600

541 aagttctcgc ggtacggact cccgatgtag gtcctcgcgt ggtagaagaa gctcctactg 600

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggcaactaca agtcgcgcgc cgaggtgaag ttcgagggcg ataccctggt gaatcgcatc 660

601 ccgttgatgt tcagcgcgcg gctccacttc aagctcccgc tatgggacca cttagcgtag 660

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gagctgaccg gcaccgattt caaggaggat ggcaacatcc tgggcaataa gatggagtac 720

661 ctcgactggc cgtggctaaa gttcctccta ccgttgtagg acccgttatt ctacctcatg 720

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aactacaacg cccacaatgt gtacatcatg accgacaagg ccaagaatgg catcaaggtg 780

721 ttgatgttgc gggtgttaca catgtagtac tggctgttcc ggttcttacc gtagttccac 780

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aacttcaaga tccgccacaa catcgaggat ggcagcgtgc agctggccga ccactaccag 840

781 ttgaagttct aggcggtgtt gtagctccta ccgtcgcacg tcgaccggct ggtgatggtc 840

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cagaataccc ccatcggcga tggccctgtg ctgctgcccg ataaccacta cctgtccacc 900

841 gtcttatggg ggtagccgct accgggacac gacgacgggc tattggtgat ggacaggtgg 900

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cagagcgccc tgtccaagga ccccaacgag aagcgcgatc acatgatcta cttcggcttc 960

901 gtctcgcggg acaggttcct ggggttgctc ttcgcgctag tgtactagat gaagccgaag 960

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtgaccgccg ccgccatcac ccacggcatg gatgagctgt acaagtgagc ggccgcgact 1020

961 cactggcggc ggcggtagtg ggtgccgtac ctactcgaca tgttcactcg ccggcgctga 1020

.......... ...... ORF 1 EGFP(0-717) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctagaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa 1080

1021 gatcttaagg ttgactcgcg gccagcgatg gtaatggttg aacagaccac agtttttatt 1080

.......... .......... .......... .......... .......... ..........

StuI(1084),SpeI(1088) ApaI(1107) ........ .......... ..........

1081 taggcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga 1140

1081 atccggatga tcagccggca tgcccgggaa agcagagcgc gcaaagccac tactgccact 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 1200

1141 tttggagact gtgtacgtcg agggcctctg ccagtgtcga acagacattc gcctacggcc 1200

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 1260

1201 ctcgtctgtt cgggcagtcc cgcgcagtcg cccacaaccg cccacagccc cgaccgaatt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NdeI(1299) .......... ..........

1261 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 1320

1261 gatacgccgt agtctcgtct aacatgactc tcacgtggta tacgccacac tttatggcgt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... ......... AflII(1356) ......... ..........

1321 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat 1380

1321 gtctacgcat tcctctttta tggcgtagtc cgccggaatt cccggagcac tatgcggata 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AatII(1422) .........

1381 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 1440

1381 aaaatatcca attacagtac tattattacc aaagaatctg cagtccaccg tgaaaagccc 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 1500

1441 ctttacacgc gccttgggga taaacaaata aaaagattta tgtaagttta tacataggcg 1500

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

1501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 1560

1501 agtactctgt tattgggact atttacgaag ttattataac tttttccttc tcatactcat 1560

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 1620

1561 aagttgtaaa ggcacagcgg gaataaggga aaaaacgccg taaaacggaa ggacaaaaac 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 1680

1621 gagtgggtct ttgcgaccac tttcattttc tacgacttct agtcaaccca cgtgctcacc 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 1740

1681 caatgtagct tgacctagag ttgtcgccat tctaggaact ctcaaaagcg gggcttcttg 1740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 1800

1741 caaaaggtta ctactcgtga aaatttcaag acgatacacc gcgccataat agggcataac 1800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 1860

1801 tgcggcccgt tctcgttgag ccagcggcgt atgtgataag agtcttactg aaccaactca 1860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 1920

1861 tgagtggtca gtgtcttttc gtagaatgcc taccgtactg tcattctctt aatacgtcac 1920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 1980

1921 gacggtattg gtactcacta ttgtgacgcc ggttgaatga agactgttgc tagcctcctg 1980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 2040

1981 gcttcctcga ttggcgaaaa aacgtgttgt accccctagt acattgagcg gaactagcaa 2040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 2100

2041 cccttggcct cgacttactt cggtatggtt tgctgctcgc actgtggtgc tacggacatc 2100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... FspI(2118) .......... .......... .......... ..........

2101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 2160

2101 gttaccgttg ttgcaacgcg tttgataatt gaccgcttga tgaatgagat cgaagggccg 2160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 2220

2161 ttgttaatta tctgacctac ctccgcctat ttcaacgtcc tggtgaagac gcgagccggg 2220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 2280

2221 aaggccgacc gaccaaataa cgactattta gacctcggcc actcgcaccc agagcgccat 2280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 2340

2281 agtaacgtcg tgaccccggt ctaccattcg ggagggcata gcatcaatag atgtgctgcc 2340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 2400

2341 cctcagtccg ttgataccta cttgctttat ctgtctagcg actctatcca cggagtgact 2400

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 2460

2401 aattcgtaac cattgacagt ctggttcaaa tgagtatata tgaaatctaa ctaaattttg 2460

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 2520

2461 aagtaaaaat taaattttcc tagatccact tctaggaaaa actattagag tactggtttt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 2580

2521 agggaattgc actcaaaagc aaggtgactc gcagtctggg gcatcttttc tagtttccta 2580

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

2581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 2640

2581 gaagaactct aggaaaaaaa gacgcgcatt agacgacgaa cgtttgtttt tttggtggcg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 2700

2641 atggtcgcca ccaaacaaac ggcctagttc tcgatggttg agaaaaaggc ttccattgac 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 2760

2701 cgaagtcgtc tcgcgtctat ggtttatgac aggaagatca catcggcatc aatccggtgg 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 2820

2761 tgaagttctt gagacatcgt ggcggatgta tggagcgaga cgattaggac aatggtcacc 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 2880

2821 gacgacggtc accgctattc agcacagaat ggcccaacct gagttctgct atcaatggcc 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 2940

2881 tattccgcgt cgccagcccg acttgccccc caagcacgtg tgtcgggtcg aacctcgctt 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 3000

2941 gctggatgtg gcttgactct atggatgtcg cactcgatac tctttcgcgg tgcgaagggc 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 3060

3001 ttccctcttt ccgcctgtcc ataggccatt cgccgtccca gccttgtcct ctcgcgtgct 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 3120

3061 ccctcgaagg tccccctttg cggaccatag aaatatcagg acagcccaaa gcggtggaga 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 3180

3121 ctgaactcgc agctaaaaac actacgagca gtccccccgc ctcggatacc tttttgcggt 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 3240

3181 cgttgcgccg gaaaaatgcc aaggaccgga aaacgaccgg aaaacgagtg tacaagaaag 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 3300

3241 gacgcaatag gggactaaga cacctattgg cataatggcg gaaactcact cgactatggc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag...... 3354

3301 gagcggcgtc ggcttgctgg ctcgcgtcgc tcagtcactc gctccttcgc cttc...... 3354

.......... .......... .......... .......... .......... ..........