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pAdEasy-1
Gene/insert name: none
Insert size (bp): Unknown
GenBank/Entrez ID of insert: AY370909
Vector backbone: pAdEasy-1 (Search Vector Database)
Type of vector: Mammalian expression,Adenoviral
Backbone size (bp): 33441
5' Sequencing primer: n/a (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH10B
Principal Investigator: Bert Vogelstein
Comments: Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy-1.
Plasmids recombined in pAdEasy-1 should be packaged in either 293 or 911 cells to make virus.
Article: A simplified system for generating recombinant adenoviruses. He TC et al. (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. Pubmed)